CA2286349A1 - Elaboration de proteines fonctionnelles : equilibre entre contrainte tangentielle et gravite - Google Patents

Elaboration de proteines fonctionnelles : equilibre entre contrainte tangentielle et gravite Download PDF

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Publication number
CA2286349A1
CA2286349A1 CA002286349A CA2286349A CA2286349A1 CA 2286349 A1 CA2286349 A1 CA 2286349A1 CA 002286349 A CA002286349 A CA 002286349A CA 2286349 A CA2286349 A CA 2286349A CA 2286349 A1 CA2286349 A1 CA 2286349A1
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CA
Canada
Prior art keywords
cell
cells
shear stress
culture
renal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002286349A
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English (en)
Inventor
Thomas John Goodwin
Timothy Grant Hammond
James Howard Kaysen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tulane University
National Aeronautics and Space Administration NASA
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Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2286349A1 publication Critical patent/CA2286349A1/fr
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/13—Decoys
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/31—Chemical structure of the backbone
    • C12N2310/315—Phosphorothioates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé relatif à l'élaboration de protéines fonctionnelles, y compris les hormones issues des cellules rénales, qui consiste à établir un processus de co-culture à trois dimensions réagissant à la contrainte tangentielle dans un récipient à parois tournantes. Le mélange naturel des cellules rénales exprime l'enzyme 1-a-hydroxylase, susceptible d'être utilisée pour engendrer la forme active de la vitamine D: 1,25-diOH vitamine D3. Les cultures de fibroblastes et la co-culture de cellules rénales corticales expriment le gène correspondant à l'érythropoïétine et secrètent l'érythropoïétine dans le surnageant de culture. D'autres gènes réagissant à la contrainte tangentielle sont également modulés sous l'effet de ladite contrainte : par exemple, récepteurs de toxines comme la mégaline et la cubuline (gp280). L'invention concerne aussi un procédé conçu pour traiter les individus nécessitant un traitement à base de protéines fonctionnelles, élaborées en processus de co-culture à trois dimensions réagissant à la contrainte tangentielle et établi dans un récipient à parois tournantes.
CA002286349A 1997-04-08 1998-04-07 Elaboration de proteines fonctionnelles : equilibre entre contrainte tangentielle et gravite Abandoned CA2286349A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US4320597P 1997-04-08 1997-04-08
US60/043,205 1997-04-08
PCT/US1998/006826 WO1998045468A1 (fr) 1997-04-08 1998-04-07 Elaboration de proteines fonctionnelles : equilibre entre contrainte tangentielle et gravite

Publications (1)

Publication Number Publication Date
CA2286349A1 true CA2286349A1 (fr) 1998-10-15

Family

ID=21926026

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002286349A Abandoned CA2286349A1 (fr) 1997-04-08 1998-04-07 Elaboration de proteines fonctionnelles : equilibre entre contrainte tangentielle et gravite

Country Status (7)

Country Link
EP (1) EP0972069A1 (fr)
JP (1) JP2001517081A (fr)
AU (1) AU6953498A (fr)
BR (1) BR9808510A (fr)
CA (1) CA2286349A1 (fr)
IL (1) IL132264A0 (fr)
WO (1) WO1998045468A1 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU1795400A (en) * 1998-12-24 2000-07-31 Florence Medical Ltd. Expression vectors comprising multiple shear stress responsive elements (ssre) and a gene of interest and methods of use thereof
CA2572326A1 (fr) * 2004-08-04 2006-02-16 Applied Research Systems Ars Holding N.V. Capsules contenant des cellules transitoirement transfectees, procede servant a preparer celles-ci et utilisations de celles-ci
JP5350364B2 (ja) * 2007-04-28 2013-11-27 ヒョンジン ヤン， 粉末状の足場を用いた細胞間の信号調節による細胞培養方法
MX2012000705A (es) * 2009-07-15 2012-03-07 Abbott Lab Mejoramiento de produccion celular a traves de mecanotransduccion.

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4988623A (en) * 1988-06-30 1991-01-29 The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration Rotating bio-reactor cell culture apparatus
WO1991011535A1 (fr) * 1990-01-30 1991-08-08 Childrens Hospital Of Los Angeles Inhibition de transcription a l'aide d'oligonucleotides bicatenaires

Also Published As

Publication number Publication date
EP0972069A1 (fr) 2000-01-19
AU6953498A (en) 1998-10-30
WO1998045468A1 (fr) 1998-10-15
BR9808510A (pt) 2000-05-23
JP2001517081A (ja) 2001-10-02
IL132264A0 (en) 2001-03-19

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