BR9808510A - Produção de proteìnas funcionais: balanço de cisalhamento e gravidade - Google Patents

Produção de proteìnas funcionais: balanço de cisalhamento e gravidade

Info

Publication number
BR9808510A
BR9808510A BR9808510-7A BR9808510A BR9808510A BR 9808510 A BR9808510 A BR 9808510A BR 9808510 A BR9808510 A BR 9808510A BR 9808510 A BR9808510 A BR 9808510A
Authority
BR
Brazil
Prior art keywords
shear
functional proteins
production
gravity
cultured
Prior art date
Application number
BR9808510-7A
Other languages
English (en)
Inventor
Thomas John Goodwin
Timothy Grant Hammond
James Howard Kaysen
Original Assignee
Nasa
Univ Tulane
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nasa, Univ Tulane filed Critical Nasa
Publication of BR9808510A publication Critical patent/BR9808510A/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/13—Decoys
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/31—Chemical structure of the backbone
    • C12N2310/315—Phosphorothioates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Patente de Invenção:<B>"PRODUçãO DE PROTEìNAS FUNCIONAIS: BALANçO DE CISALHAMENTO E GRAVIDADE"<D>. A presente invenção proporciona um método para a produção de proteínas funcionais, incluindo os hormónios, por células renais, em um processo de cultivo em conjunto, tridimensional, sensível ao cisalhamento, usando um vaso com parede rotativo. A mistura natural de células renais expressa a enzima 1-a-hidroxilase, a qual pode ser usada para gerar a forma ativa da vitamina D: a 1,25-diOH vitamina D3. As culturas de fibroblastos e o cultivo em conjunto de células corticais renais expressam o gene para a eritropoetina e secretam a eritropoetina no sobrenadante da cultura. Outros genes de resposta ao cisalhamento são também modulados por cisalhamento, tais como os receptores para toxina megalina e cubulina (gp280). Também proporcionado é um método de tratar indivíduo necessitado com as proteínas funcionais produzidas em um processo de cultivo em conjunto, tridimensional, sensível ao cisalhamento, usando um vaso com parede rotativo.
BR9808510-7A 1997-04-08 1998-04-07 Produção de proteìnas funcionais: balanço de cisalhamento e gravidade BR9808510A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US4320597P 1997-04-08 1997-04-08
PCT/US1998/006826 WO1998045468A1 (en) 1997-04-08 1998-04-07 Production of functional proteins: balance of shear stress and gravity

Publications (1)

Publication Number Publication Date
BR9808510A true BR9808510A (pt) 2000-05-23

Family

ID=21926026

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9808510-7A BR9808510A (pt) 1997-04-08 1998-04-07 Produção de proteìnas funcionais: balanço de cisalhamento e gravidade

Country Status (7)

Country Link
EP (1) EP0972069A1 (pt)
JP (1) JP2001517081A (pt)
AU (1) AU6953498A (pt)
BR (1) BR9808510A (pt)
CA (1) CA2286349A1 (pt)
IL (1) IL132264A0 (pt)
WO (1) WO1998045468A1 (pt)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1141266A4 (en) * 1998-12-24 2002-09-25 Florence Medical Ltd EXPRESSION VECTORS, WHICH MULTIPLE, SHEAR RESPECTING ELEMENTS (SSRE) INCLUDE AND A GENE AND METHOD FOR THEIR APPLICATION
AU2005270968A1 (en) * 2004-08-04 2006-02-16 Merck Serono Sa Capsules containing transfected cells, method for preparing the same and uses thereof for immunization and vaccination
JP5350364B2 (ja) * 2007-04-28 2013-11-27 ヒョンジン ヤン， 粉末状の足場を用いた細胞間の信号調節による細胞培養方法
EP2454373B1 (en) * 2009-07-15 2014-11-19 AbbVie Inc. Enhancement of cellular production through mechanotransduction

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4988623A (en) * 1988-06-30 1991-01-29 The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration Rotating bio-reactor cell culture apparatus
WO1991011535A1 (en) * 1990-01-30 1991-08-08 Childrens Hospital Of Los Angeles Inhibition of transcription by double-stranded oligonucleotides

Also Published As

Publication number Publication date
AU6953498A (en) 1998-10-30
CA2286349A1 (en) 1998-10-15
IL132264A0 (en) 2001-03-19
WO1998045468A1 (en) 1998-10-15
JP2001517081A (ja) 2001-10-02
EP0972069A1 (en) 2000-01-19

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Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

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B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO PUBLICADO NA RPI 1942 DE 25 - 03 - 2008.