CA2284481A1 - Procede de realisation d'echantillotheque soustractive - Google Patents

Procede de realisation d'echantillotheque soustractive Download PDF

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Publication number
CA2284481A1
CA2284481A1 CA002284481A CA2284481A CA2284481A1 CA 2284481 A1 CA2284481 A1 CA 2284481A1 CA 002284481 A CA002284481 A CA 002284481A CA 2284481 A CA2284481 A CA 2284481A CA 2284481 A1 CA2284481 A1 CA 2284481A1
Authority
CA
Canada
Prior art keywords
library
sequences
dna
subtraction
isolated
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002284481A
Other languages
English (en)
Inventor
Paul S. Kayne
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SmithKline Beecham Corp
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2284481A1 publication Critical patent/CA2284481A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Abstract

La présente invention concerne un procédé de réalisation d'échantillothèque soustractive par utilisation d'une collection de séquences définies. Ce procédé consiste à disposer d'une surface contenant une collection constituée de séquences connues d'acides nucléiques qui sont ensuite mise en contact avec une échantillothèque contenant des séquences non définies dans des conditions d'hybridation appropriée. Le procédé consiste ensuite à récupérer et séquencer tout ADN non hybridé. L'ADN séquencé résultant non hybridé vient ainsi former une échantillothèque soustractive contenant des séquences qui étaient présentes dans l'échantillothèque, mais qui sont différentes des séquences de la collection. L'invention concerne également une échantillothèque soustractive obtenue selon le procédé de l'invention. L'invention concerne enfin des procédés de réalisation et d'utilisation des sondes obtenues par soustraction.
CA002284481A 1997-03-24 1998-03-24 Procede de realisation d'echantillotheque soustractive Abandoned CA2284481A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US4168897P 1997-03-24 1997-03-24
US60/041,688 1997-03-24
PCT/US1998/005644 WO1998043088A1 (fr) 1997-03-24 1998-03-24 Procede de realisation d'echantillotheque soustractive

Publications (1)

Publication Number Publication Date
CA2284481A1 true CA2284481A1 (fr) 1998-10-01

Family

ID=21917812

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002284481A Abandoned CA2284481A1 (fr) 1997-03-24 1998-03-24 Procede de realisation d'echantillotheque soustractive

Country Status (4)

Country Link
EP (1) EP1005653A4 (fr)
JP (1) JP2002507275A (fr)
CA (1) CA2284481A1 (fr)
WO (1) WO1998043088A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6638717B2 (en) 1999-05-19 2003-10-28 Aventis Pharmaceuticals, Inc. Microarray-based subtractive hybridzation

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04502107A (ja) * 1988-12-07 1992-04-16 ザ・ジェネラル・ホスピタル・コーポレーション 挿入を有するかまたは欠失に相当するｄｎａの豊富化およびクローニングの方法
EP0533952A4 (en) * 1991-04-15 1993-07-07 Sumitomo Electric Industries, Ltd. Process for separating or extracting nucleic acid from specimen containing the same
JP3645903B2 (ja) * 1992-03-04 2005-05-11 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 比較ゲノムハイブリダイゼーション（ｃｇｈ）
US5591575A (en) * 1993-04-07 1997-01-07 Amersham International Plc Subtraction hybridization employing aziridinylbenoquinone cross-linking agents

Also Published As

Publication number Publication date
JP2002507275A (ja) 2002-03-05
WO1998043088A1 (fr) 1998-10-01
EP1005653A4 (fr) 2004-05-06
EP1005653A1 (fr) 2000-06-07

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