EP1005653A4 - Procede de realisation d'echantillotheque soustractive - Google Patents

Procede de realisation d'echantillotheque soustractive

Info

Publication number
EP1005653A4
EP1005653A4 EP98913043A EP98913043A EP1005653A4 EP 1005653 A4 EP1005653 A4 EP 1005653A4 EP 98913043 A EP98913043 A EP 98913043A EP 98913043 A EP98913043 A EP 98913043A EP 1005653 A4 EP1005653 A4 EP 1005653A4
Authority
EP
European Patent Office
Prior art keywords
producing
subtraction library
subtraction
library
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP98913043A
Other languages
German (de)
English (en)
Other versions
EP1005653A1 (fr
Inventor
Paul S Kayne
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SmithKline Beecham Corp
Original Assignee
SmithKline Beecham Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SmithKline Beecham Corp filed Critical SmithKline Beecham Corp
Publication of EP1005653A1 publication Critical patent/EP1005653A1/fr
Publication of EP1005653A4 publication Critical patent/EP1005653A4/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809Methods for determination or identification of nucleic acids involving differential detection
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
EP98913043A 1997-03-24 1998-03-24 Procede de realisation d'echantillotheque soustractive Withdrawn EP1005653A4 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US4168897P 1997-03-24 1997-03-24
US41688P 1997-03-24
PCT/US1998/005644 WO1998043088A1 (fr) 1997-03-24 1998-03-24 Procede de realisation d'echantillotheque soustractive

Publications (2)

Publication Number Publication Date
EP1005653A1 EP1005653A1 (fr) 2000-06-07
EP1005653A4 true EP1005653A4 (fr) 2004-05-06

Family

ID=21917812

Family Applications (1)

Application Number Title Priority Date Filing Date
EP98913043A Withdrawn EP1005653A4 (fr) 1997-03-24 1998-03-24 Procede de realisation d'echantillotheque soustractive

Country Status (4)

Country Link
EP (1) EP1005653A4 (fr)
JP (1) JP2002507275A (fr)
CA (1) CA2284481A1 (fr)
WO (1) WO1998043088A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6638717B2 (en) 1999-05-19 2003-10-28 Aventis Pharmaceuticals, Inc. Microarray-based subtractive hybridzation

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0372524A2 (fr) * 1988-12-07 1990-06-13 The General Hospital Corporation Procédé pour l'enrichissement et le clonage d'ADN équivalent à une insertion ou une délétion
EP0533952A1 (fr) * 1991-04-15 1993-03-31 Sumitomo Electric Industries, Ltd. Procede de separation ou d'extraction d'acide nucleique a partir d'un echantillon contenant celui-ci
WO1993018186A1 (fr) * 1992-03-04 1993-09-16 The Regents Of The University Of California Hybridation genomique comparative

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5591575A (en) * 1993-04-07 1997-01-07 Amersham International Plc Subtraction hybridization employing aziridinylbenoquinone cross-linking agents

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0372524A2 (fr) * 1988-12-07 1990-06-13 The General Hospital Corporation Procédé pour l'enrichissement et le clonage d'ADN équivalent à une insertion ou une délétion
EP0533952A1 (fr) * 1991-04-15 1993-03-31 Sumitomo Electric Industries, Ltd. Procede de separation ou d'extraction d'acide nucleique a partir d'un echantillon contenant celui-ci
WO1993018186A1 (fr) * 1992-03-04 1993-09-16 The Regents Of The University Of California Hybridation genomique comparative

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
HARA E ET AL: "Subtractive cDNA cloning using oligo(dT)30-latex and PCR: isolation of cDNA clones specific to undifferentiated human embryonal carcinoma cells", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 19, no. 25, December 1991 (1991-12-01), pages 7097 - 7104, XP002209142, ISSN: 0305-1048 *
LOPEZ-FERNANDEZ L A ET AL: "CONSTRUCTION OF SUBTRACTIVE CDNA LIBRARIES FROM LIMITED AMOUNTS OF MRNA AND MULTIPLE CYCLES OF SUBTRACTION", BIOTECHNIQUES, EATON PUBLISHING, NATICK, US, vol. 15, no. 4, 1 October 1993 (1993-10-01), pages 654,656,658 - 659, XP000402907, ISSN: 0736-6205 *
ROBERTSON N G ET AL: "ISOLATION OF NOVEL AND KNOWN GENES FROM A HUMAN FETAL COCHLEAR CDNA LIBRARY USING SUBSTRACTIVE HYBRIDIZATION AND DIFFERENTIAL SCREENING", GENOMICS, ACADEMIC PRESS, SAN DIEGO, US, vol. 23, no. 1, 1 September 1994 (1994-09-01), pages 42 - 50, XP001012412, ISSN: 0888-7543 *
See also references of WO9843088A1 *

Also Published As

Publication number Publication date
JP2002507275A (ja) 2002-03-05
EP1005653A1 (fr) 2000-06-07
WO1998043088A1 (fr) 1998-10-01
CA2284481A1 (fr) 1998-10-01

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Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

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17P Request for examination filed

Effective date: 19991001

AK Designated contracting states

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Designated state(s): BE CH DE ES FR GB IT LI NL

A4 Supplementary search report drawn up and despatched

Effective date: 20040318

RIC1 Information provided on ipc code assigned before grant

Ipc: 7C 12N 15/10 B

Ipc: 7C 12Q 1/68 B

Ipc: 7G 01N 33/566 A

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20040504