EP1005653A4 - Method of producing a subtraction library - Google Patents

Method of producing a subtraction library

Info

Publication number
EP1005653A4
EP1005653A4 EP98913043A EP98913043A EP1005653A4 EP 1005653 A4 EP1005653 A4 EP 1005653A4 EP 98913043 A EP98913043 A EP 98913043A EP 98913043 A EP98913043 A EP 98913043A EP 1005653 A4 EP1005653 A4 EP 1005653A4
Authority
EP
European Patent Office
Prior art keywords
producing
subtraction library
subtraction
library
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP98913043A
Other languages
German (de)
French (fr)
Other versions
EP1005653A1 (en
Inventor
Paul S Kayne
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SmithKline Beecham Corp
Original Assignee
SmithKline Beecham Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SmithKline Beecham Corp filed Critical SmithKline Beecham Corp
Publication of EP1005653A1 publication Critical patent/EP1005653A1/en
Publication of EP1005653A4 publication Critical patent/EP1005653A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809Methods for determination or identification of nucleic acids involving differential detection
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
EP98913043A 1997-03-24 1998-03-24 Method of producing a subtraction library Withdrawn EP1005653A4 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US4168897P 1997-03-24 1997-03-24
US41688P 1997-03-24
PCT/US1998/005644 WO1998043088A1 (en) 1997-03-24 1998-03-24 Method of producing a subtraction library

Publications (2)

Publication Number Publication Date
EP1005653A1 EP1005653A1 (en) 2000-06-07
EP1005653A4 true EP1005653A4 (en) 2004-05-06

Family

ID=21917812

Family Applications (1)

Application Number Title Priority Date Filing Date
EP98913043A Withdrawn EP1005653A4 (en) 1997-03-24 1998-03-24 Method of producing a subtraction library

Country Status (4)

Country Link
EP (1) EP1005653A4 (en)
JP (1) JP2002507275A (en)
CA (1) CA2284481A1 (en)
WO (1) WO1998043088A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6638717B2 (en) 1999-05-19 2003-10-28 Aventis Pharmaceuticals, Inc. Microarray-based subtractive hybridzation

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0372524A2 (en) * 1988-12-07 1990-06-13 The General Hospital Corporation Method of enrichment and cloning for DNA containing an insertion or corresponding to a deletion
EP0533952A1 (en) * 1991-04-15 1993-03-31 Sumitomo Electric Industries, Ltd. Process for separating or extracting nucleic acid from specimen containing the same
WO1993018186A1 (en) * 1992-03-04 1993-09-16 The Regents Of The University Of California Comparative genomic hybridization (cgh)

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5591575A (en) * 1993-04-07 1997-01-07 Amersham International Plc Subtraction hybridization employing aziridinylbenoquinone cross-linking agents

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0372524A2 (en) * 1988-12-07 1990-06-13 The General Hospital Corporation Method of enrichment and cloning for DNA containing an insertion or corresponding to a deletion
EP0533952A1 (en) * 1991-04-15 1993-03-31 Sumitomo Electric Industries, Ltd. Process for separating or extracting nucleic acid from specimen containing the same
WO1993018186A1 (en) * 1992-03-04 1993-09-16 The Regents Of The University Of California Comparative genomic hybridization (cgh)

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
HARA E ET AL: "Subtractive cDNA cloning using oligo(dT)30-latex and PCR: isolation of cDNA clones specific to undifferentiated human embryonal carcinoma cells", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 19, no. 25, December 1991 (1991-12-01), pages 7097 - 7104, XP002209142, ISSN: 0305-1048 *
LOPEZ-FERNANDEZ L A ET AL: "CONSTRUCTION OF SUBTRACTIVE CDNA LIBRARIES FROM LIMITED AMOUNTS OF MRNA AND MULTIPLE CYCLES OF SUBTRACTION", BIOTECHNIQUES, EATON PUBLISHING, NATICK, US, vol. 15, no. 4, 1 October 1993 (1993-10-01), pages 654,656,658 - 659, XP000402907, ISSN: 0736-6205 *
ROBERTSON N G ET AL: "ISOLATION OF NOVEL AND KNOWN GENES FROM A HUMAN FETAL COCHLEAR CDNA LIBRARY USING SUBSTRACTIVE HYBRIDIZATION AND DIFFERENTIAL SCREENING", GENOMICS, ACADEMIC PRESS, SAN DIEGO, US, vol. 23, no. 1, 1 September 1994 (1994-09-01), pages 42 - 50, XP001012412, ISSN: 0888-7543 *
See also references of WO9843088A1 *

Also Published As

Publication number Publication date
JP2002507275A (en) 2002-03-05
WO1998043088A1 (en) 1998-10-01
EP1005653A1 (en) 2000-06-07
CA2284481A1 (en) 1998-10-01

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Legal Events

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PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

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Effective date: 20040504