CA2277545A1 - Bioconjugaison d'oligonucleotides - Google Patents

Bioconjugaison d'oligonucleotides Download PDF

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Publication number
CA2277545A1
CA2277545A1 CA002277545A CA2277545A CA2277545A1 CA 2277545 A1 CA2277545 A1 CA 2277545A1 CA 002277545 A CA002277545 A CA 002277545A CA 2277545 A CA2277545 A CA 2277545A CA 2277545 A1 CA2277545 A1 CA 2277545A1
Authority
CA
Canada
Prior art keywords
gap
bioconjugates
group
teg
rna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002277545A
Other languages
English (en)
Inventor
Andrew W. Stephens
C. Michael Willis
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Proligo LLC
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2277545A1 publication Critical patent/CA2277545A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Saccharide Compounds (AREA)

Abstract

Cette invention concerne un nouveau procédé permettant de conjuguer des oligonucléotides d'ARN avec d'autres entités moléculaires exclusivement en position 5' de l'oligonucléotide. Cette invention concerne particulièrement un procédé permettant de conjuguer ou de former un dérivé d'oligonucléotides d'ARN par transcription, en présence d'une guanosine modifiée en 5'. L'invention concerne enfin les nouveaux oligonucléotides bioconjugués pouvant être préparés conformément au procédé de cette invention.
CA002277545A 1997-01-08 1998-01-07 Bioconjugaison d'oligonucleotides Abandoned CA2277545A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US3465197P 1997-01-08 1997-01-08
US60/034,651 1997-01-08
US5820697P 1997-09-08 1997-09-08
US60/058,206 1997-09-08
PCT/US1998/000589 WO1998030720A1 (fr) 1997-01-08 1998-01-07 Bioconjugaison d'oligonucleotides

Publications (1)

Publication Number Publication Date
CA2277545A1 true CA2277545A1 (fr) 1998-07-16

Family

ID=67296859

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002277545A Abandoned CA2277545A1 (fr) 1997-01-08 1998-01-07 Bioconjugaison d'oligonucleotides

Country Status (5)

Country Link
EP (1) EP1015629A1 (fr)
JP (1) JP2001508657A (fr)
AU (1) AU6022798A (fr)
CA (1) CA2277545A1 (fr)
WO (1) WO1998030720A1 (fr)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6346611B1 (en) 1990-06-11 2002-02-12 Gilead Sciences, Inc. High affinity TGfβ nucleic acid ligands and inhibitors
AU2001296645A1 (en) 2000-10-06 2002-04-15 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding dna and rna
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
DK1401853T3 (da) 2001-05-25 2010-11-01 Univ Duke Modulatorer af farmakologiske midler
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
GB0129012D0 (en) 2001-12-04 2002-01-23 Solexa Ltd Labelled nucleotides
US7414116B2 (en) 2002-08-23 2008-08-19 Illumina Cambridge Limited Labelled nucleotides
EP3002289B1 (fr) 2002-08-23 2018-02-28 Illumina Cambridge Limited Nucleotides modifies pour le sequençage de polynucleotide
US11008359B2 (en) 2002-08-23 2021-05-18 Illumina Cambridge Limited Labelled nucleotides
US20040197804A1 (en) * 2002-12-03 2004-10-07 Keefe Anthony D. Method for in vitro selection of 2'-substituted nucleic acids
CA2551495A1 (fr) * 2004-01-07 2005-07-28 Ambit Biosciences Corporation Petites molecules conjuguees
FR2889700B1 (fr) * 2005-08-11 2012-11-23 Synthinnove Lab Marqueurs, leur procede de fabrication et leurs applications
GB0517097D0 (en) 2005-08-19 2005-09-28 Solexa Ltd Modified nucleosides and nucleotides and uses thereof
EP2071927A2 (fr) 2006-09-28 2009-06-24 Illumina, Inc. Compositions et procédés de séquencage nucléotidique
GB2457402B (en) 2006-12-01 2011-10-19 Univ Columbia Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
EP3431615A3 (fr) 2007-10-19 2019-02-20 The Trustees of Columbia University in the City of New York Séquençage d'adn avec des terminateurs nucléotidiques réversibles non fluorescents et terminateurs nucléotidiques modifiées à étiquette clivable
US20110014611A1 (en) 2007-10-19 2011-01-20 Jingyue Ju Design and synthesis of cleavable fluorescent nucleotides as reversible terminators for dna sequences by synthesis
LT2852602T (lt) * 2012-05-21 2020-10-26 Agilent Technologies, Inc. Oligonukleotidų konjugavimo būdas
JP2016512846A (ja) * 2013-03-13 2016-05-09 ダナ−ファーバー キャンサー インスティテュート， インコーポレイテッド Ｒａｓ阻害剤およびその使用
BR112015022448B1 (pt) 2013-03-15 2020-12-08 Illumina Cambridge Limited molécula de nucleotídeo ou nucleosídeo modificado, métodos para preparar o crescimento de polinucleotídeo complementar a polinucleotídeo alvo de fita simples em reação de sequenciação e para determinar a sequência de polinucleotídeo alvo de fita simples e kit
CN114249786A (zh) * 2021-12-29 2022-03-29 上海彩迩文生化科技有限公司 含n,n-二酰基结构核苷中间体的制备和应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5688935A (en) * 1990-06-11 1997-11-18 Nexstar Pharmaceuticals, Inc. Nucleic acid ligands of tissue target
US5712375A (en) * 1990-06-11 1998-01-27 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: tissue selex

Also Published As

Publication number Publication date
EP1015629A1 (fr) 2000-07-05
JP2001508657A (ja) 2001-07-03
AU6022798A (en) 1998-08-03
WO1998030720A1 (fr) 1998-07-16

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