CA2264487A1 - Polypeptides solubles a activite de serine protease ns3 du virus de l'hepatite c et leur procede de preparation et d'isolement - Google Patents
Polypeptides solubles a activite de serine protease ns3 du virus de l'hepatite c et leur procede de preparation et d'isolement Download PDFInfo
- Publication number
- CA2264487A1 CA2264487A1 CA002264487A CA2264487A CA2264487A1 CA 2264487 A1 CA2264487 A1 CA 2264487A1 CA 002264487 A CA002264487 A CA 002264487A CA 2264487 A CA2264487 A CA 2264487A CA 2264487 A1 CA2264487 A1 CA 2264487A1
- Authority
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- Canada
- Prior art keywords
- polypeptides
- hcv
- protease
- serine protease
- protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/503—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
- C12N9/506—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses derived from RNA viruses
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- Enzymes And Modification Thereof (AREA)
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
La présente invention concerne la sérine protéase NS3 du virus de l'hépatite C et notamment l'observation que le domaine de la sérine protéase NS3, dans sa conformation native, lie un ion Zn?2+¿ et que des ions métalliques bivalents sont nécessaires à l'intégrité structurelle de la protéine et à l'activité de l'enzyme. La présente invention concerne également des polypeptides recombinés comprenant des séquences de la protéase SN3 et caractérisés par une queue d'au moins trois lysines à leur extrémité C-terminale afin d'en augmenter la solubilité. Un autre sujet de l'invention àtrait à un nouveau procédé permettant l'expression desdits polypeptides, sous la forme de métalloprotéines, avec l'activité protéolyptique de la protéase SN3 du virus de l'hépatite C en une forme soluble et en une quantité suffisante pour permettre de rechercher à identifier des inhibiteurs et déterminer la structure tridimensionnelle de la protéase SN3. La figure 4 illustre les effets de l'ion zinc sur la production de la protéase SN3 du virus de l'hépatite C en tant que protéine soluble dans E.Coli dans un milieu de culture minimum.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ITRM96A000632 | 1996-09-17 | ||
| ITRM960632 IT1285158B1 (it) | 1996-09-17 | 1996-09-17 | Polipeptidi solubili con l'attivita' di serino-proteasi di ns3 del virus dell'epatite c, e procedimento per la loro preparazione e il |
| PCT/IT1997/000228 WO1998012308A1 (fr) | 1996-09-17 | 1997-09-17 | Polypeptides solubles a activite de serine protease ns3 du virus de l'hepatite c et leur procede de preparation et d'isolement |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2264487A1 true CA2264487A1 (fr) | 1998-03-26 |
Family
ID=11404423
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002264487A Abandoned CA2264487A1 (fr) | 1996-09-17 | 1997-09-17 | Polypeptides solubles a activite de serine protease ns3 du virus de l'hepatite c et leur procede de preparation et d'isolement |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP0950094A1 (fr) |
| JP (1) | JP2001500735A (fr) |
| AU (1) | AU4397097A (fr) |
| CA (1) | CA2264487A1 (fr) |
| IT (1) | IT1285158B1 (fr) |
| WO (1) | WO1998012308A1 (fr) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9806815D0 (en) * | 1998-03-30 | 1998-05-27 | Hoffmann La Roche | Amino acid derivatives |
| EP1141255A4 (fr) | 1999-01-08 | 2002-09-11 | Bristol Myers Squibb Co | Formes modifiees de la protease ns3 du virus de l'hepatite c |
| CZ2003195A3 (cs) | 2000-07-21 | 2003-04-16 | Schering Corporation | Peptidové inhibitory serinové proteázy NS3 a farmaceutický prostředek |
| BR0112540A (pt) | 2000-07-21 | 2003-06-24 | Schering Corp | Peptìdios como inibidores da ns-3-serina protease do vìrus da hepatite c |
| US7244721B2 (en) | 2000-07-21 | 2007-07-17 | Schering Corporation | Peptides as NS3-serine protease inhibitors of hepatitis C virus |
| US7494660B2 (en) | 2003-10-27 | 2009-02-24 | Vertex Pharmaceuticals Incorporated | HCV NS3-NS4A protease resistance mutants |
| US7705138B2 (en) | 2005-11-11 | 2010-04-27 | Vertex Pharmaceuticals Incorporated | Hepatitis C virus variants |
| HUE029110T2 (en) | 2009-07-31 | 2017-02-28 | Baxalta GmbH | Cell culture medium for ADAMTS protein expression |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0571554A1 (fr) * | 1991-01-14 | 1993-12-01 | James N. Gamble Institute Of Medical Research | Polypeptides immunogeniques structuraux de base ayant des epitopes pour vhc, anticorps, sequences de polynucleotides, vaccins et methodes |
| US5843752A (en) * | 1995-05-12 | 1998-12-01 | Schering Corporation | Soluble active hepatitis C virus protease |
| IT1277914B1 (it) * | 1995-08-22 | 1997-11-12 | Angeletti P Ist Richerche Bio | Procedimento per produrre - in forma pura e in quantita' elevate - polipeptidi con l'attivita' proteolitica della proteasi ns3 di hcv, e |
-
1996
- 1996-09-17 IT ITRM960632 patent/IT1285158B1/it active IP Right Grant
-
1997
- 1997-09-17 EP EP97942190A patent/EP0950094A1/fr not_active Withdrawn
- 1997-09-17 CA CA002264487A patent/CA2264487A1/fr not_active Abandoned
- 1997-09-17 JP JP10514467A patent/JP2001500735A/ja active Pending
- 1997-09-17 WO PCT/IT1997/000228 patent/WO1998012308A1/fr not_active Application Discontinuation
- 1997-09-17 AU AU43970/97A patent/AU4397097A/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| WO1998012308A1 (fr) | 1998-03-26 |
| ITRM960632A1 (it) | 1998-03-17 |
| JP2001500735A (ja) | 2001-01-23 |
| AU4397097A (en) | 1998-04-14 |
| IT1285158B1 (it) | 1998-06-03 |
| EP0950094A1 (fr) | 1999-10-20 |
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| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| FZDE | Discontinued |