CA2259983A1 - Transformation of zygote, egg or sperm cells, and regeneration of transformed plants from isolated embryo sacs - Google Patents

Transformation of zygote, egg or sperm cells, and regeneration of transformed plants from isolated embryo sacs Download PDF

Info

Publication number
CA2259983A1
CA2259983A1 CA002259983A CA2259983A CA2259983A1 CA 2259983 A1 CA2259983 A1 CA 2259983A1 CA 002259983 A CA002259983 A CA 002259983A CA 2259983 A CA2259983 A CA 2259983A CA 2259983 A1 CA2259983 A1 CA 2259983A1
Authority
CA
Canada
Prior art keywords
plant
transformed
embryo
isolated
gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002259983A
Other languages
English (en)
French (fr)
Inventor
David D. Cass
William J. Gordon-Kamm
Guichang Zhang
Locksley Mcgann
Jerome P. Ranch
John D. Laurie
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Alberta
Pioneer Hi Bred International Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2259983A1 publication Critical patent/CA2259983A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA002259983A 1996-07-08 1997-07-08 Transformation of zygote, egg or sperm cells, and regeneration of transformed plants from isolated embryo sacs Abandoned CA2259983A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US2132596P 1996-07-08 1996-07-08
US60/021,325 1996-07-08
US60/021,325(CON) 1996-07-08

Publications (1)

Publication Number Publication Date
CA2259983A1 true CA2259983A1 (en) 1998-01-15

Family

ID=21803574

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002259983A Abandoned CA2259983A1 (en) 1996-07-08 1997-07-08 Transformation of zygote, egg or sperm cells, and regeneration of transformed plants from isolated embryo sacs

Country Status (9)

Country Link
US (1) US6300543B1 (en:Method)
EP (1) EP0914455B1 (en:Method)
AR (1) AR007731A1 (en:Method)
AT (1) ATE299188T1 (en:Method)
AU (2) AU3495297A (en:Method)
CA (1) CA2259983A1 (en:Method)
DE (1) DE69733688T2 (en:Method)
WO (2) WO1998001575A1 (en:Method)
ZA (1) ZA976074B (en:Method)

Families Citing this family (85)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7135608B1 (en) * 1997-08-28 2006-11-14 The Salk Institute For Biological Studies Site-specific recombination in eukaryotes and constructs useful therefor
HUP9900603A1 (hu) * 1999-03-12 2000-12-28 MTA Mezőgazdasági Kutatóintézete Eljárás és berendezés magasabbrendű növények polarizálható sejtjeinek immobilizálására nagy frekvenciájú váltóáram alkalmazásával
WO2002079495A2 (en) * 2001-03-27 2002-10-10 University Of Delaware Genomics applications for modified oligonucleotides
JP2006509726A (ja) * 2002-08-07 2006-03-23 ユニバーシティー、オブ、デラウェア タンパク質のミスアセンブリおよび凝集を示す、疾患の処置のための、組成物および方法
EP1502955A1 (en) * 2003-08-01 2005-02-02 Institut für Pflanzengenetik und Kulturpflanzenforschung Methods for the production of stably transformed, fertile gramineae employing agrobacterium-mediated transformation of isolated gramineae zygotes
US7560611B2 (en) 2003-08-05 2009-07-14 Monsanto Technology Llc Method and apparatus for substantially isolating plant tissues
CA2541914C (en) 2003-10-09 2012-07-24 E.I. Du Pont De Nemours And Company Gene silencing by using micro-rna molecules
US20060123518A1 (en) * 2004-12-01 2006-06-08 Pioneer Hi-Bred International, Inc. Methods for culturing cereal endosperm
EP1896595A2 (en) 2005-05-25 2008-03-12 Pioneer-Hi-Bred International, Inc. Methods for improving crop plant architecture and yield
BRPI0612862A2 (pt) 2005-07-18 2010-11-30 Pioneer Hi Bred Int polinucleotìdeo isolado, método para obtenção de uma célula transformada, método para obtenção de uma planta transformada, método de produção de recombinação de dna de sìtio especìfico, biblioteca isolada, método de geração de biblioteca de moléculas e método para selecionar sìtio de recombinação frt modificado
US8859846B2 (en) 2005-09-21 2014-10-14 E. I. Du Pont De Nemours And Company Doubling of chromosomes in haploid embryos
US20070143881A1 (en) * 2005-12-16 2007-06-21 Pioneer Hi-Bred International, Inc. Methods and Compositions for Improving the Efficiency of Site-Specific Polynucleotide Exchange
US20070143880A1 (en) * 2005-12-16 2007-06-21 Pioneer Hi-Bred International, Inc. Methods for Introducing Into a Plant a Polynucleotide of Interest
EP2730587A3 (en) 2006-02-09 2014-08-27 Pioneer Hi-Bred International, Inc. Genes for enhancing nitrogen utilization efficiency in crop plants
BRPI0712398A2 (pt) 2006-05-17 2012-10-16 Pioneer Hi-Bred International minicromossomo artificial de planta, planta de milho, polinucleotìdeo isolado, construção recombinante, planta transgênica de milho e método para produzir uma planta transgênica de milho compreendendo um minicromossomo artificial de planta tendo centrÈmero funcional
WO2008045460A2 (en) 2006-10-05 2008-04-17 E. I. Du Pont De Nemours And Company Maize microrna sequences
WO2008112645A2 (en) 2007-03-09 2008-09-18 Monsanto Technology Llc Methods for plant transformation using spectinomycin selection
US8912392B2 (en) 2007-06-29 2014-12-16 Pioneer Hi-Bred International, Inc. Methods for altering the genome of a monocot plant cell
US8115055B2 (en) 2007-12-18 2012-02-14 E.I. Du Pont De Nemours And Company Down-regulation of gene expression using artificial microRNAs
US8937217B2 (en) 2007-12-18 2015-01-20 E. I. Du Pont De Nemours And Company Down-regulation of gene expression using artificial microRNAs
CA2741006A1 (en) 2008-10-30 2010-05-20 Pioneer Hi-Bred International, Inc. Manipulation of glutamine synthetases (gs) to improve nitrogen use efficiency and grain yield in higher plants
CN102395674B (zh) 2009-04-14 2015-07-29 先锋国际良种公司 调节acc合酶改善低氮条件下的植物产量
MX2012001080A (es) 2009-07-24 2012-03-26 Pionner Hi Bred International Inc Uso de agrupaciones de componentes del dominio de dimerizacion para modular la arquitectura de las plantas.
IN2012DN01315A (en:Method) 2009-08-20 2015-06-05 Pioneer Hi Bred Int
EP2467395A1 (en) 2009-08-20 2012-06-27 Pioneer Hi-Bred International Inc. Functional expression of shuffled yeast nitrate transporter (ynti) in maize to improve nitrate uptake under low nitrate environment
MX2012003972A (es) 2009-10-02 2012-05-08 Pionner Hi Bred International Inc Regulacion a la baja de acc sintasa para mejorar el rendimiento de las plantas.
CN102906267A (zh) 2010-01-06 2013-01-30 先锋国际良种公司 玉米光合作用组织和非光合作用组织昼夜节律的鉴定及其在改良作物方面的用途
BR112012027150A2 (pt) 2010-04-23 2015-09-15 Du Pont método para regular a expressão em uma célula vegetal, interruptor gênico, planta transgênica, célula vegetal transgênica ou semente transgênica e polinucleotídeo recombinante
US8903660B2 (en) 2010-06-24 2014-12-02 The Invention Science Fund I, Llc Rejuvenation or preservation of germ cells
US8338178B2 (en) 2010-10-28 2012-12-25 The Invention Science Fund I, Llc Mitochondrial enhancement of cells
MX2013010911A (es) 2011-03-23 2015-03-03 Pioneer Hi Bred Int Metodos para producir un locus de rasgo transgenico complejo.
BR112013027860A2 (pt) 2011-04-29 2016-11-29 Pioneer Hi Bred Int ácido nucleico isolado, planta ou célula vegetal, método para aumentar a capacidade do dreno, acelerar a remobilização e/ou a senescência e a secagem de grão em uma planta, método para aumentar a tolerância a seca na ausência de uma penalidade de produtividade sob condições sem seca, cassete de expressão e método para reduzir a expressão de zmme293 ou a atividade de zmme293 em uma planta
US9499837B2 (en) 2011-06-21 2016-11-22 E.I. Du Pont De Nemours And Company Methods and compositions for producing male sterile plants
WO2013066805A1 (en) 2011-10-31 2013-05-10 Pioneer Hi-Bred International, Inc. Improving plant drought tolerance, nitrogen use efficiency and yield
US20150089689A1 (en) 2012-01-23 2015-03-26 E I Du Pont Nemours And Company Down-regulation of gene expression using artificial micrornas for silencing fatty acid biosynthetic genes
WO2013138309A1 (en) 2012-03-13 2013-09-19 Pioneer Hi-Bred International, Inc. Genetic reduction of male fertility in plants
CN104703998B (zh) 2012-03-13 2020-08-21 先锋国际良种公司 植物中雄性育性的遗传减少
WO2013158966A1 (en) 2012-04-20 2013-10-24 Futuragene Israel Ltd. Bronze bug control agents
WO2013163085A2 (en) 2012-04-23 2013-10-31 Futuragene Israel Ltd. Glycaspis brimblecombei control agents
US20150240253A1 (en) 2012-08-30 2015-08-27 E. I. Du Pont De Nemours And Company Long intergenic non-coding rnas in maize
UY35064A (es) 2012-10-03 2014-04-30 Futuragene Israel Ltd Agentes para el control de la avispa de las agallas
US20150299718A1 (en) 2012-11-20 2015-10-22 Rajeev Gupta Engineering Plants for Efficient Uptake and Utilization of Urea to Improve
WO2014164014A1 (en) 2013-03-11 2014-10-09 Pioneer Hi-Bred International, Inc. Genes for improving nutrient uptake and abiotic stress tolerance in plants
US20160010101A1 (en) 2013-03-13 2016-01-14 Pioneer Hi-Bred International, Inc. Enhanced nitrate uptake and nitrate translocation by over- expressing maize functional low-affinity nitrate transporters in transgenic maize
US20160017360A1 (en) 2013-03-13 2016-01-21 Pioneer Hi-Bred International, Inc. Functional expression of bacterial major facilitator superfamily mfs gene in maize to improve agronomic traits and grain yield
WO2014160122A1 (en) 2013-03-14 2014-10-02 Pioneer Hi-Bred International, Inc. Maize stress related transcription factor 18 and uses thereof
US20160017350A1 (en) 2013-03-15 2016-01-21 Pioneer Hi-Bred International, Inc. Compositions and methods of use of acc oxidase polynucleotides and polypeptides
US20150024497A1 (en) * 2013-07-19 2015-01-22 Elwha Llc Methods and Systems for Utilizing Sperm for Molecular Delivery
BR112016003561B8 (pt) 2013-08-22 2022-11-01 Du Pont Método para a produção de uma modificação genética, método para a introdução de um polinucleotídeo de interesse no genoma de uma planta, método para a edição de um segundo gene em um genoma de planta e método para gerar uma planta de milho resistente ao glifosato
CA2954626A1 (en) 2014-07-11 2016-01-14 E. I. Du Pont De Nemours And Company Compositions and methods for producing plants resistant to glyphosate herbicide
MX2017010746A (es) 2015-02-25 2017-11-28 Pioneer Hi Bred Int Composicion y metodos para la expresion regulada de un complejo de arn guia/endonucleasa cas.
CA2985506A1 (en) 2015-05-15 2016-11-24 Pioneer Hi-Bred International, Inc. Guide rna/cas endonuclease systems
CA2999050C (en) 2015-10-12 2024-05-28 E. I. Du Pont De Nemours And Company Protected dna templates for gene modification and increased homologous recombination in cells and methods of use
BR112018008109A2 (pt) 2015-10-20 2018-11-06 Pioneer Hi Bred Int métodos para modificar uma sequência de nucleotídeos no genoma de uma célula vegetal, produzir uma planta, produzir tecido de calo de planta que tem uma sequência de nucleotídeos modificada em seu genoma sem o uso de um marcador selecionável, e planta de progênie da planta
BR112018007796A2 (pt) 2015-11-06 2018-10-30 Du Pont plantas de soja, partes de plantas de soja ou sementes de soja, método para selecionar uma célula de soja, métodos de seleção de uma célula de soja e de produção de um locus e molécula de ácido nucleico
DK3387134T3 (da) 2015-12-11 2020-12-21 Danisco Us Inc Fremgangsmåder og sammensætninger til øget nukleasemedieret genommodifikation og reducerede virkninger uden for målstedet
EP3708665A1 (en) 2015-12-18 2020-09-16 Danisco US Inc. Methods and compositions for t-rna based guide rna expression
FI3390632T3 (fi) 2015-12-18 2025-11-25 Danisco Us Inc Menetelmät ja koostumukset polymeraasi ii (pol-ii) -pohjaisen opas-rna:n ilmentämiseen
US20190161742A1 (en) 2016-03-11 2019-05-30 Pioneer Hi-Bred International, Inc. Novel cas9 systems and methods of use
EP3699280A3 (en) 2016-03-11 2020-11-18 Pioneer Hi-Bred International, Inc. Novel cas9 systems and methods of use
WO2017155715A1 (en) 2016-03-11 2017-09-14 Pioneer Hi-Bred International, Inc. Novel cas9 systems and methods of use
US12133495B2 (en) 2016-03-18 2024-11-05 Pioneer Hi-Bred International, Inc. Methods and compositions for producing clonal, non-reduced, non-recombined gametes
EP3228187A1 (en) 2016-04-06 2017-10-11 Kws Saat Se Novel means and methods for cereal pathogen resistance
WO2017184500A1 (en) 2016-04-18 2017-10-26 Bloomsburg University of Pennsylvania Compositions and methods of delivering molecules to plants
CA3022854A1 (en) 2016-06-14 2017-12-21 Pioneer Hi-Bred International, Inc. Use of cpf1 endonuclease for plant genome modifications
US20190100745A1 (en) 2016-06-20 2019-04-04 Pioneer Hi-Bred International, Inc. Novel cas systems and methods of use
WO2018060881A1 (en) 2016-09-27 2018-04-05 University Of Florida Research Foundation, Inc. Insect toxin delivery mediated by a densovirus coat protein
JP2020072645A (ja) * 2017-01-31 2020-05-14 日本たばこ産業株式会社 植物に物質を導入する方法
WO2018202800A1 (en) 2017-05-03 2018-11-08 Kws Saat Se Use of crispr-cas endonucleases for plant genome engineering
CA3074154A1 (en) 2017-08-29 2019-03-07 KWS SAAT SE & Co. KGaA Improved blue aleurone and other segregation systems
WO2019053725A1 (en) 2017-09-18 2019-03-21 Futuragene Israel Ltd. TISSUE-SPECIFIC EXPRESSION CONTROL OF DELLA POLYPEPTIDES
CN111465689B (zh) 2017-12-15 2024-07-12 丹尼斯科美国公司 Cas9变体和使用方法
US11926835B1 (en) 2018-01-29 2024-03-12 Inari Agriculture Technology, Inc. Methods for efficient tomato genome editing
US20220010293A1 (en) 2018-02-23 2022-01-13 Pioneer Hi-Bred International, Inc. Novel cas9 orthologs
US11866719B1 (en) 2018-06-04 2024-01-09 Inari Agriculture Technology, Inc. Heterologous integration of regulatory elements to alter gene expression in wheat cells and wheat plants
WO2020005667A1 (en) 2018-06-29 2020-01-02 Pioneer Hi-Bred International, Inc. Compositions and methods for editing an endogenous nac gene in plants
US12365888B2 (en) 2018-12-14 2025-07-22 Pioneer Hi-Bred International, Inc. CRISPR-Cas systems for genome editing
JP2022526414A (ja) 2019-04-05 2022-05-24 ダニスコ・ユーエス・インク 二重環状組換えDNAコンストラクトを使用するバチルス(Bacillus)のゲノムへのポリヌクレオチド組込みのための方法及びその組成物
MX2021012158A (es) 2019-04-05 2022-01-06 Danisco Us Inc Métodos para la integración de una secuencia de adn donante en el genoma de bacillus usando constructos de adn recombinante lineal y composiciones de los mismos.
EP4110905A4 (en) 2020-02-24 2024-03-27 Pioneer Hi-Bred International, Inc. Intra-genomic homologous recombination
CN118742647A (zh) 2022-02-15 2024-10-01 富优基尼以色列股份有限公司 对植物保护有用的苏云金芽孢杆菌杀虫蛋白(Bt PP)组合
WO2024118882A1 (en) 2022-12-01 2024-06-06 Genencor International Bv Iterative multiplex genome engineering in microbial cells using a selection marker swapping system
WO2024118876A1 (en) 2022-12-01 2024-06-06 Genencor International Bv Iterative multiplex genome engineering in microbial cells using a recombinant self-excisable selection marker system
EP4627078A1 (en) 2022-12-01 2025-10-08 Danisco US Inc. Iterative muliplex genome engineering in microbial cells using a bidirectional selection marker system
AU2024239266A1 (en) 2023-03-20 2025-09-25 Pioneer Hi-Bred International, Inc. Cas polypeptides with altered pam recognition

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0275069A3 (en) * 1987-01-13 1990-04-25 DNA PLANT TECHNOLOGY CORPORATION (under the laws of the state of Delaware) Pollen-mediated gene transformation in plants
FI890917L (fi) * 1988-03-02 1989-09-03 Eidgenoess Tech Hochschule Foerfarande foer framstaellning av transgena vaexter.
US5225341A (en) 1990-07-19 1993-07-06 The Regents Of The University Of California Biologically safe plant transformation system using a ds transposon
AU4539593A (en) * 1992-06-23 1994-01-24 South Dakota State University Transformation of plants by direct injection of dna
DK94892A (da) * 1992-07-23 1994-01-24 Carlsberg Forskningscenter Fremgangsmåde til at regenerere protoplaster, celler eller cellevæv fra planter til hele planter, medium til brug for regenerering, fremgangsmåder til at isolere ægprotoplaster og synergideprotoplaster fra frøanlæg, de ved fremgangsmåderne isolerede ægprotoplaster og synergideprotoplaster samt fremgangsmåde til transformering af befrugtede ægcelle

Also Published As

Publication number Publication date
EP0914455A1 (en) 1999-05-12
US6300543B1 (en) 2001-10-09
AU3495297A (en) 1998-02-02
EP0914455B1 (en) 2005-07-06
AU3718697A (en) 1998-02-02
WO1998001576A1 (en) 1998-01-15
AU731406C (en) 2002-03-21
DE69733688D1 (de) 2005-08-11
WO1998001575A1 (en) 1998-01-15
AR007731A1 (es) 1999-11-10
AU731406B2 (en) 2001-03-29
DE69733688T2 (de) 2006-04-27
ATE299188T1 (de) 2005-07-15
ZA976074B (en) 1999-04-14

Similar Documents

Publication Publication Date Title
US6300543B1 (en) Transformation of zygote, egg or sperm cells and recovery of transformed plants from isolated embryo sacs
WO1998001576A9 (en) Transformation of zygote, egg or sperm cells and recovery of transformed plants from isolated embryo sacs
US20250115924A1 (en) Systems and methods for cellular reprogramming of a plant cell
AU730927B2 (en) Use of the green fluorescent protein as a screenable marker for plant transformation
AU747636B2 (en) Novel nucleic acid sequence encoding FLP recombinase
AU2020259484A1 (en) Embryogenesis factors for cellular reprogramming of a plant cell
US7148402B2 (en) Promotion of somatic embryogenesis in plants by PGA37 gene expression
JP2013074892A (ja) 半数体植物胚および倍加半数体植物胚を作出する方法
CA3197681A1 (en) Parthenogenesis factors and methods of using same
US20230407324A1 (en) Doubled haploid inducer
US20020004939A1 (en) Methods for identifying transgenic plants using morphological markers
Roeckel et al. Plant transformation using the sexual route
CN120738280A (zh) 基于单倍体诱导系的植物遗传转化方法
HK40034402A (en) Systems and methods for cellular reprogramming of a plant cell

Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued