ATE299188T1 - Transformation der zygote und regeneration der transformierten maispflanzen aus isolierten embryosäcken - Google Patents

Transformation der zygote und regeneration der transformierten maispflanzen aus isolierten embryosäcken

Info

Publication number
ATE299188T1
ATE299188T1 AT97934024T AT97934024T ATE299188T1 AT E299188 T1 ATE299188 T1 AT E299188T1 AT 97934024 T AT97934024 T AT 97934024T AT 97934024 T AT97934024 T AT 97934024T AT E299188 T1 ATE299188 T1 AT E299188T1
Authority
AT
Austria
Prior art keywords
embryosacks
zygote
regeneration
transformation
isolated
Prior art date
Application number
AT97934024T
Other languages
English (en)
Inventor
David D Cass
Locksley E Mcgann
Guichang Zhang
John D Laurie
Jerome P Ranch
William J Gordon-Kamm
Original Assignee
Pioneer Hi Bred Int
Univ Alberta
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi Bred Int, Univ Alberta filed Critical Pioneer Hi Bred Int
Application granted granted Critical
Publication of ATE299188T1 publication Critical patent/ATE299188T1/de

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
AT97934024T 1996-07-08 1997-07-08 Transformation der zygote und regeneration der transformierten maispflanzen aus isolierten embryosäcken ATE299188T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US2132596P 1996-07-08 1996-07-08
PCT/US1997/011184 WO1998001576A1 (en) 1996-07-08 1997-07-08 Transformation of zygote, egg or sperm cells and recovery of transformed plants from isolated embryo sacs

Publications (1)

Publication Number Publication Date
ATE299188T1 true ATE299188T1 (de) 2005-07-15

Family

ID=21803574

Family Applications (1)

Application Number Title Priority Date Filing Date
AT97934024T ATE299188T1 (de) 1996-07-08 1997-07-08 Transformation der zygote und regeneration der transformierten maispflanzen aus isolierten embryosäcken

Country Status (9)

Country Link
US (1) US6300543B1 (de)
EP (1) EP0914455B1 (de)
AR (1) AR007731A1 (de)
AT (1) ATE299188T1 (de)
AU (2) AU3495297A (de)
CA (1) CA2259983A1 (de)
DE (1) DE69733688T2 (de)
WO (2) WO1998001575A1 (de)
ZA (1) ZA976074B (de)

Families Citing this family (78)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7135608B1 (en) * 1997-08-28 2006-11-14 The Salk Institute For Biological Studies Site-specific recombination in eukaryotes and constructs useful therefor
HUP9900603A1 (hu) * 1999-03-12 2000-12-28 MTA Mezőgazdasági Kutatóintézete Eljárás és berendezés magasabbrendű növények polarizálható sejtjeinek immobilizálására nagy frekvenciájú váltóáram alkalmazásával
WO2002079495A2 (en) * 2001-03-27 2002-10-10 University Of Delaware Genomics applications for modified oligonucleotides
JP2006509726A (ja) * 2002-08-07 2006-03-23 ユニバーシティー、オブ、デラウェア タンパク質のミスアセンブリおよび凝集を示す、疾患の処置のための、組成物および方法
EP1502955A1 (de) * 2003-08-01 2005-02-02 Institut für Pflanzengenetik und Kulturpflanzenforschung Verfahren zur Herstellung von stabil transformierten, fertilen Gramineae durch Agrobakterium-vermittelten Gentransfer von isolierten Zygoten
US7560611B2 (en) 2003-08-05 2009-07-14 Monsanto Technology Llc Method and apparatus for substantially isolating plant tissues
AU2004280634A1 (en) 2003-10-09 2005-04-21 E. I. Du Pont De Nemours And Company Gene silencing by using micro-RNA molecules
US20060123518A1 (en) * 2004-12-01 2006-06-08 Pioneer Hi-Bred International, Inc. Methods for culturing cereal endosperm
EP1896595A2 (de) 2005-05-25 2008-03-12 Pioneer-Hi-Bred International, Inc. Verfahren zur verbesserung des aufbaus und des ertrags von kulturpflanzen
US7736897B2 (en) 2005-07-18 2010-06-15 Pioneer Hi-Bred International, Inc. FRT recombination sites and methods of use
US8859846B2 (en) 2005-09-21 2014-10-14 E. I. Du Pont De Nemours And Company Doubling of chromosomes in haploid embryos
US20070143880A1 (en) * 2005-12-16 2007-06-21 Pioneer Hi-Bred International, Inc. Methods for Introducing Into a Plant a Polynucleotide of Interest
US20070143881A1 (en) * 2005-12-16 2007-06-21 Pioneer Hi-Bred International, Inc. Methods and Compositions for Improving the Efficiency of Site-Specific Polynucleotide Exchange
AU2007212168A1 (en) 2006-02-09 2007-08-16 Pioneer Hi-Bred International, Inc. Genes for enhancing nitrogen utilization efficiency in crop plants
CN101490267B (zh) 2006-05-17 2013-04-17 先锋高级育种国际公司 人工植物微染色体
CN101522901A (zh) 2006-10-05 2009-09-02 纳幕尔杜邦公司 玉米微rna序列
EP3290520B1 (de) 2007-03-09 2021-07-14 Monsanto Technology LLC Zubereitung und verwendung von embryoexplantaten aus pflanzen für die transformation
WO2009006297A2 (en) 2007-06-29 2009-01-08 Pioneer Hi-Bred International, Inc. Methods for altering the genome of a monocot plant cell
US8937217B2 (en) 2007-12-18 2015-01-20 E. I. Du Pont De Nemours And Company Down-regulation of gene expression using artificial microRNAs
US8115055B2 (en) 2007-12-18 2012-02-14 E.I. Du Pont De Nemours And Company Down-regulation of gene expression using artificial microRNAs
EP2344640A1 (de) 2008-10-30 2011-07-20 Pioneer Hi-Bred International Inc. Manipulation von glutamin-synthetasen (gs) zur verbesserung der stickstoffnutzungseffizienz und kornausbeute bei höheren pflanzen
UA108071C2 (uk) 2009-04-14 2015-03-25 Піонер Хай-Бред Інтернешнл, Інк. Спосіб поліпшення витривалості до нестачі азоту у рослини
IN2012DN00531A (de) 2009-07-24 2015-08-21 Pioneer Hi Bred Int
EP2467472A2 (de) 2009-08-20 2012-06-27 Pioneer Hi-Bred International Inc. Funktionale expression eines hefenitrattransporters (ynt1) bei mais zur verbesserung der nitrataufnahme
CA2770854A1 (en) 2009-08-20 2011-02-24 Pioneer Hi-Bred International, Inc. Functional expression of shuffled yeast nitrate transporter (ynt1) in maize to improve nitrate uptake under low nitrate environment
EP2483404A1 (de) 2009-10-02 2012-08-08 Pioneer Hi-Bred International Inc. Herunterregulierung von acc-synthase für erhöhte pflanzenleistung
CN102906267A (zh) 2010-01-06 2013-01-30 先锋国际良种公司 玉米光合作用组织和非光合作用组织昼夜节律的鉴定及其在改良作物方面的用途
AU2011243085A1 (en) 2010-04-23 2012-10-11 E. I. Du Pont De Nemours And Company Gene switch compositions and methods of use
US8527209B2 (en) 2010-06-24 2013-09-03 The Invention Science Fund I, Llc Rejuvenation or preservation of germ cells
US8846028B2 (en) 2010-10-28 2014-09-30 The Invention Science Fund I, Llc Mitochondrial enhancement of cells
CN103842511A (zh) 2011-03-23 2014-06-04 先锋国际良种公司 产生复合转基因性状基因座的方法
CA2833876A1 (en) 2011-04-29 2012-11-01 Pioneer Hi-Bred International, Inc. Down-regulation of a homeodomain-leucine zipper i-class homeobox gene for improved plant performance
US9499837B2 (en) 2011-06-21 2016-11-22 E.I. Du Pont De Nemours And Company Methods and compositions for producing male sterile plants
WO2013066805A1 (en) 2011-10-31 2013-05-10 Pioneer Hi-Bred International, Inc. Improving plant drought tolerance, nitrogen use efficiency and yield
US9598701B2 (en) 2012-01-23 2017-03-21 E I Du Pont De Nemours And Company Down-regulation of gene expression using artificial MicroRNAs for silencing fatty acid biosynthetic genes
CN104703998B (zh) 2012-03-13 2020-08-21 先锋国际良种公司 植物中雄性育性的遗传减少
WO2013138309A1 (en) 2012-03-13 2013-09-19 Pioneer Hi-Bred International, Inc. Genetic reduction of male fertility in plants
CN104395474A (zh) 2012-04-20 2015-03-04 富优基尼以色列股份有限公司 青铜蝽防治剂
BR112014026363A2 (pt) 2012-04-23 2017-06-27 Futuragene Israel Ltd molécula de ácido ribonucleico, dsrna, vetor, célula hospedeira, tecido vegetal, ácido nucleico isolado, método de produção de planta resistente a pragas e método de inibição de infestação de pragas
US20150240253A1 (en) 2012-08-30 2015-08-27 E. I. Du Pont De Nemours And Company Long intergenic non-coding rnas in maize
CN104903449A (zh) 2012-10-03 2015-09-09 富优基尼以色列股份有限公司 瘿蜂防治剂
CA2889557A1 (en) 2012-11-20 2014-05-30 Pioneer Hi-Bred International., Inc. Engineering plants for efficient uptake and utilization of urea to improve crop production
WO2014164014A1 (en) 2013-03-11 2014-10-09 Pioneer Hi-Bred International, Inc. Genes for improving nutrient uptake and abiotic stress tolerance in plants
WO2014164116A1 (en) 2013-03-13 2014-10-09 Pioneer Hi-Bred International, Inc. Functional expression of bacterial major facilitator superfamily (sfm) gene in maize to improve agronomic traits and grain yield
WO2014164074A1 (en) 2013-03-13 2014-10-09 Pioneer Hi-Bred International, Inc. Enhanced nitrate uptake and nitrate translocation by over-expressing maize functional low-affinity nitrate transporters in transgenic maize
BR112015023304A2 (pt) 2013-03-14 2017-07-18 Pioneer Hi Bred Int método para melhorar a tolerância ao estresse abiótico de uma planta, planta e semente
BR112015023700A2 (pt) 2013-03-15 2017-07-18 Pioneer Hi Bred Int método de aprimoramento da tolerância ao estresse abiótico em uma planta de cultura agrícola , planta de milho transgênica, célula vegetal, semente, método de regulação negativa de um gene de acc oxidase endógeno em uma planta de milho.
US20150024497A1 (en) * 2013-07-19 2015-01-22 Elwha Llc Methods and Systems for Utilizing Sperm for Molecular Delivery
LT3036327T (lt) 2013-08-22 2019-06-25 Pioneer Hi-Bred International, Inc. Genomo modifikacijos, panaudojant nukreipiančias polinukleotido/cas endonukleazės sistemas, ir panaudojimo būdai
WO2016007347A1 (en) 2014-07-11 2016-01-14 E. I. Du Pont De Nemours And Company Compositions and methods for producing plants resistant to glyphosate herbicide
MX2017010746A (es) 2015-02-25 2017-11-28 Pioneer Hi Bred Int Composicion y metodos para la expresion regulada de un complejo de arn guia/endonucleasa cas.
CN107709562A (zh) 2015-05-15 2018-02-16 先锋国际良种公司 指导rna/cas内切核酸酶系统
DK3362560T3 (da) 2015-10-12 2022-11-07 Dupont Us Holding Llc Beskyttede dna-templater til genmodificering og forøget homolog rekombination i celler og fremgangsmåder til anvendelse
AU2016341041A1 (en) 2015-10-20 2018-03-15 Pioneer Hi-Bred International, Inc. Methods and compositions for marker-free genome modification
BR112018007796A2 (pt) 2015-11-06 2018-10-30 Du Pont plantas de soja, partes de plantas de soja ou sementes de soja, método para selecionar uma célula de soja, métodos de seleção de uma célula de soja e de produção de um locus e molécula de ácido nucleico
US20180362961A1 (en) 2015-12-11 2018-12-20 Danisco Us Inc. Methods and compositions for enhanced nuclease-mediated genome modification and reduced off-target site effects
EP3390632A1 (de) 2015-12-18 2018-10-24 Danisco US Inc. Verfahren und zusammensetzungen für polymerase ii (pol-ii)-basierte guide-rna-expression
WO2017105991A1 (en) 2015-12-18 2017-06-22 Danisco Us Inc. Methods and compositions for t-rna based guide rna expression
WO2017155715A1 (en) 2016-03-11 2017-09-14 Pioneer Hi-Bred International, Inc. Novel cas9 systems and methods of use
EP3426780A1 (de) 2016-03-11 2019-01-16 Pioneer Hi-Bred International, Inc. Neuartige cas9-systeme und verfahren zur verwendung
CA3010628A1 (en) 2016-03-11 2017-09-14 Pioneer Hi-Bred International, Inc. Novel cas9 systems and methods of use
AU2017234920B2 (en) 2016-03-18 2023-08-17 E. I. Du Pont De Nemours And Company Methods and compositions for producing clonal, non-reduced, non-recombined gametes
EP3228187A1 (de) 2016-04-06 2017-10-11 Kws Saat Se Neuartige mittel und verfahren für getreidekrankheitserregerresistenz
US10563216B2 (en) 2016-04-18 2020-02-18 Bloomsburg University of Pennsylvania Compositions and methods of delivering molecules to plants
BR112018076027A2 (pt) 2016-06-14 2019-03-26 Pioneer Hi-Bred International, Inc. método para modificar uma sequência-alvo no genoma de uma célula vegetal; método para editar uma sequência de nucleotídeos no genoma de uma célula vegetal; método para modificar simultaneamente múltiplas sequências-alvo no genoma de uma célula vegetal; método para modificar uma sequênciaalvo de dna no genoma de uma célula vegetal e modelo de modificação de polinucleotídeo
WO2017222773A1 (en) 2016-06-20 2017-12-28 Pioneer Hi-Bred International, Inc. Novel cas systems and methods of use
US11140902B2 (en) 2016-09-27 2021-10-12 University Of Florida Research Foundation, Inc. Insect toxin delivery mediated by a densovirus coat protein
JP2020072645A (ja) * 2017-01-31 2020-05-14 日本たばこ産業株式会社 植物に物質を導入する方法
EP3619305A1 (de) 2017-05-03 2020-03-11 KWS SAAT SE & Co. KGaA Verwendung von crispr-cas-endonuklease zum pflanzengenom-engineering
US11697822B2 (en) 2017-08-29 2023-07-11 KWS SAAT SE & Co. KGaA Blue aleurone and other segregation systems
BR112020004268A2 (pt) 2017-09-18 2020-09-29 Futuragene Israel Ltd. construto, célula hospedeira, tecido vegetal, método para produzir uma planta, planta transgênica, método para engenheirar uma planta, planta, e, célula vegetal
KR20200098578A (ko) 2017-12-15 2020-08-20 다니스코 유에스 인크. Cas9 변이체 및 사용 방법
US11926835B1 (en) 2018-01-29 2024-03-12 Inari Agriculture Technology, Inc. Methods for efficient tomato genome editing
US11866719B1 (en) 2018-06-04 2024-01-09 Inari Agriculture Technology, Inc. Heterologous integration of regulatory elements to alter gene expression in wheat cells and wheat plants
WO2020005667A1 (en) 2018-06-29 2020-01-02 Pioneer Hi-Bred International, Inc. Compositions and methods for editing an endogenous nac gene in plants
EP3947662A1 (de) 2019-04-05 2022-02-09 Danisco US Inc. Verfahren zur integration einer donor-dna-sequenz in das genom von bacillus unter verwendung linearer rekombinanter dna-konstrukte und zusammensetzungen davon
US20220162621A1 (en) 2019-04-05 2022-05-26 Danisco Us Inc Methods For Polynucleotide Integration Into The Genome Of Bacillus Using Dual Circular Recombinant DNA Constructs And Compositions Thereof
WO2023156906A1 (en) 2022-02-15 2023-08-24 Futuragene Israel Ltd A bacillus thuringiensis pesticidal protein (bt pp) combination useful for plant protection

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0275069A3 (de) * 1987-01-13 1990-04-25 DNA PLANT TECHNOLOGY CORPORATION (under the laws of the state of Delaware) Gentransformation in Pflanzen mittels Pollen
FI890917A (fi) * 1988-03-02 1989-09-03 Eidgenoess Tech Hochschule Foerfarande foer framstaellning av transgena vaexter.
US5225341A (en) 1990-07-19 1993-07-06 The Regents Of The University Of California Biologically safe plant transformation system using a ds transposon
WO1994000583A1 (en) * 1992-06-23 1994-01-06 South Dakota State University Transformation of plants by direct injection of dna
DK94892A (da) * 1992-07-23 1994-01-24 Carlsberg Forskningscenter Fremgangsmåde til at regenerere protoplaster, celler eller cellevæv fra planter til hele planter, medium til brug for regenerering, fremgangsmåder til at isolere ægprotoplaster og synergideprotoplaster fra frøanlæg, de ved fremgangsmåderne isolerede ægprotoplaster og synergideprotoplaster samt fremgangsmåde til transformering af befrugtede ægcelle

Also Published As

Publication number Publication date
AU3718697A (en) 1998-02-02
EP0914455B1 (de) 2005-07-06
CA2259983A1 (en) 1998-01-15
ZA976074B (en) 1999-04-14
AU3495297A (en) 1998-02-02
WO1998001575A1 (en) 1998-01-15
EP0914455A1 (de) 1999-05-12
US6300543B1 (en) 2001-10-09
DE69733688D1 (de) 2005-08-11
AU731406C (en) 2002-03-21
AR007731A1 (es) 1999-11-10
DE69733688T2 (de) 2006-04-27
AU731406B2 (en) 2001-03-29
WO1998001576A1 (en) 1998-01-15

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