CA2247980C - Transformations des plastides chez arabidopsis thaliana - Google Patents

Transformations des plastides chez arabidopsis thaliana Download PDF

Info

Publication number
CA2247980C
CA2247980C CA002247980A CA2247980A CA2247980C CA 2247980 C CA2247980 C CA 2247980C CA 002247980 A CA002247980 A CA 002247980A CA 2247980 A CA2247980 A CA 2247980A CA 2247980 C CA2247980 C CA 2247980C
Authority
CA
Canada
Prior art keywords
plastid
cells
plant
dna
medium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA002247980A
Other languages
English (en)
Other versions
CA2247980A1 (fr
Inventor
Pal Maliga
Samir R. Sikdar
Siva Vanga Reddy
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Rutgers State University of New Jersey
Original Assignee
Rutgers State University of New Jersey
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rutgers State University of New Jersey filed Critical Rutgers State University of New Jersey
Publication of CA2247980A1 publication Critical patent/CA2247980A1/fr
Application granted granted Critical
Publication of CA2247980C publication Critical patent/CA2247980C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Steroid Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Fertilizers (AREA)

Abstract

L'invention concerne des méthodes et des compositions permettant d'obtenir des plants d'Arabidopsis et de Brassica transplastomiques. Spécifiquement, la méthode permet d'obtenir des protocoles de culture et des compositions qui facilitent la régénération de plants transformés après administration de molécules d'ADN exogènes bénéfiques.
CA002247980A 1996-03-06 1997-03-06 Transformations des plastides chez arabidopsis thaliana Expired - Fee Related CA2247980C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US1291696P 1996-03-06 1996-03-06
US60/012,916 1996-03-06
PCT/US1997/003444 WO1997032977A1 (fr) 1996-03-06 1997-03-06 Transformations des plastides chez arabidopsis thaliana

Publications (2)

Publication Number Publication Date
CA2247980A1 CA2247980A1 (fr) 1997-09-12
CA2247980C true CA2247980C (fr) 2005-05-31

Family

ID=21757358

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002247980A Expired - Fee Related CA2247980C (fr) 1996-03-06 1997-03-06 Transformations des plastides chez arabidopsis thaliana

Country Status (10)

Country Link
US (1) US6376744B1 (fr)
EP (1) EP0885298B1 (fr)
JP (1) JP2000506019A (fr)
CN (1) CN1114692C (fr)
AT (1) ATE301187T1 (fr)
AU (1) AU732479B2 (fr)
BR (1) BR9707948A (fr)
CA (1) CA2247980C (fr)
DE (1) DE69733881T2 (fr)
WO (1) WO1997032977A1 (fr)

Families Citing this family (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5767373A (en) 1994-06-16 1998-06-16 Novartis Finance Corporation Manipulation of protoporphyrinogen oxidase enzyme activity in eukaryotic organisms
US6084155A (en) * 1995-06-06 2000-07-04 Novartis Ag Herbicide-tolerant protoporphyrinogen oxidase ("protox") genes
WO1999005265A2 (fr) * 1997-07-23 1999-02-04 Sanford Scientific, Inc. Transformation amelioree de plastes de plantes superieures et production de plantes transgeniques resistantes aux herbicides
US20040045056A1 (en) * 1998-02-26 2004-03-04 Zhizheng Chen Transformation of Brassica
CN1222621C (zh) * 1998-03-11 2005-10-12 辛根塔参与股份公司 新的植物质体启动子序列
US6362398B1 (en) 1998-03-11 2002-03-26 Syngenta Participations Ag ClpP plastid promoter sequence
JP5414136B2 (ja) * 1998-08-03 2014-02-12 ラトガーズ、ザ・ステイト・ユニバーシテイ・オブ・ニユー・ジヤージー 高等植物のプラスチド中にて高レベルの蛋白を発現するための翻訳調節エレメントおよびその使用法
IL142041A0 (en) * 1998-10-07 2002-03-10 Syngenta Participations Ag Therapeutically active proteins in plants
US6515206B1 (en) 1998-12-23 2003-02-04 Calgene Llc Plastid transformation of Brassica
DE60037200T2 (de) 1999-09-21 2008-09-25 Rutgers, The State University Of New Jersey Orts-spezifisches rekombinationssystem zur manipulation des plastidengenoms in höheren pflanzen
US7186560B2 (en) * 1999-09-21 2007-03-06 Rutgers, The State University Of New Jersey High level expression of immunogenic proteins in the plastids of higher plants
US20040253586A1 (en) * 1999-12-08 2004-12-16 Siva Reddy Plastid transformation
ES2304381T3 (es) 2000-03-22 2008-10-16 Icon Genetics, Inc. Procedimiento de transformacion de plastidios de plantas y procedimiento de obtencion de plantas transplastomicas.
US20050198704A1 (en) * 2000-10-20 2005-09-08 Board Of Trustees Of Michigan State University Chloroplast transgenesis of monocots: bioconfined genetically engineered monocot crops that will eliminate transgene flow
AU2002252525A1 (en) * 2001-03-29 2002-10-15 Rutgers, The University Of New Jersey Integrases for the insertion of heterologous nucleic acids into the plastid genome
AU2002360986A1 (en) 2001-12-20 2003-07-09 Sungene Gmbh And Co. Kgaa Methods for the transformation of vegetal plastids
WO2004016793A2 (fr) * 2002-08-08 2004-02-26 Rijk Zwaan Zaadteelt En Zaadhandel B.V. Procede de transformation de plastes dans l'espece asteraceae, vecteur utilise a cet effet et vegetaux ainsi obtenus
ES2381667T3 (es) 2002-10-15 2012-05-30 Syngenta Participations Ag Transformación de plastos
US7176355B2 (en) * 2002-12-13 2007-02-13 Rutgers, The State University Of New Jersey Plastid rRNA operon promoter elements for construction of chimeric promoters for transgene expression
WO2004078935A2 (fr) * 2003-03-03 2004-09-16 Rutgers, The State University Of New Jersey Elimination de sequences de plastides a l'aide de recombinases specifiques de sites, a expression transitoire
US20050044593A1 (en) * 2003-07-01 2005-02-24 Biolex, Inc. Chloroplast transformation of duckweed
CN1322131C (zh) * 2003-09-27 2007-06-20 中国科学院植物研究所 禾谷类植物基因敲除操作平台质粒及其构建方法与应用
ATE495264T1 (de) 2003-10-06 2011-01-15 Syngenta Participations Ag In pflanzenplastiden funktionsfähiger promotor
WO2005070066A2 (fr) 2004-01-08 2005-08-04 Dow Agrosciences Llc Cultures cellulaires totipotentes chlorophylliennes
BRPI0608829A2 (pt) 2005-04-19 2011-03-15 Basf Plant Science Gmbh método para a expressão transgênica com especificidade intensificada em uma planta, uso de um construto de ácido nucleico quimérico, seqüência de ribonucleotìdeo quimérica, construto de expressão, vetor de expressão, organismo não-humano ou célula transformada, semente transformada, e, preparação farmacêutica
US7842856B2 (en) 2005-08-25 2010-11-30 The Board Of Trustees Of The University Of Illinois Herbicide resistance gene, compositions and methods
US7671254B2 (en) 2005-08-25 2010-03-02 The Board Of Trustees Of The University Of Illinois Herbicide resistance gene, compositions and methods
WO2007039454A1 (fr) 2005-09-20 2007-04-12 Basf Plant Science Gmbh Methodes de regulation de l'expression genique utilisant ta-siarn
KR100740694B1 (ko) * 2006-02-09 2007-07-18 한국생명공학연구원 색소체 게놈 내에 삽입된 외래유전자의 이차 재조합을방지하기 위한 벡터개발
US8143474B2 (en) * 2006-04-18 2012-03-27 Rutgers, The State University Of New Jersey Compositions and methods for increasing transgene expression in the plastids of higher plants
ES2382408T3 (es) 2007-02-06 2012-06-08 Basf Plant Science Gmbh Uso de genes de la alanina racemasa para conferir a las plantas resistencia a los nemátodos
BRPI0808389A2 (pt) 2007-03-15 2014-07-08 Basf Plant Science Gmbh Planta transgênica, semente, vetor de expressão, e, método para aumentar resistência a nematódeo em uma planta.
CA2684923C (fr) 2007-05-02 2015-01-06 Merial Limited Plasmides a adn a expression et stabilite ameliorees
WO2010023186A1 (fr) 2008-08-27 2010-03-04 Basf Plant Science Gmbh Plantes transgéniques résistant au nématode
EP2527450A1 (fr) 2008-12-11 2012-11-28 BASF Plant Science GmbH Résistance de nématode spécifique aux racines de plante
AR074941A1 (es) 2009-01-07 2011-02-23 Bayer Cropscience Sa Plantas transplastomicas exentas de marcador de seleccion
CA2770572A1 (fr) 2009-08-25 2011-03-03 Basf Plant Science Company Gmbh Plantes transgeniques resistantes aux nematodes
CN102051376B (zh) * 2010-01-27 2013-03-20 华中农业大学 基于油菜子叶叶绿体转化的组织培养体系及获得转化植株的方法
CA2822004A1 (fr) 2010-12-20 2012-06-28 Basf Plant Science Company Gmbh Plantes transgeniques resistantes aux nematodes
US11377663B1 (en) * 2011-08-30 2022-07-05 Monsanto Technology Llc Genetic regulatory elements
WO2013050593A1 (fr) 2011-10-07 2013-04-11 Basf Plant Science Company Gmbh Procédé de production de plantes présentant une résistance accrue à des pathogènes
WO2013050611A1 (fr) 2011-10-07 2013-04-11 Basf Plant Science Company Gmbh Procédé de production de plantes présentant une résistance accrue à des pathogènes
WO2013050318A1 (fr) 2011-10-07 2013-04-11 Basf Plant Science Company Gmbh Procédé de production de plantes ayant une résistance accrue à des pathogènes
WO2013053711A1 (fr) 2011-10-10 2013-04-18 Basf Plant Science Company Gmbh Procédé de production de plantes ayant une résistance accrue aux pathogènes
WO2013053686A1 (fr) 2011-10-10 2013-04-18 Basf Plant Science Company Gmbh Procédé de production de plantes ayant une résistance accrue à des pathogènes
CA2888596A1 (fr) * 2012-10-24 2014-05-01 Arnold Jay Bendich Transformation de plastide au moyen de vecteurs d'adn lineaire
CN103966255A (zh) * 2014-05-27 2014-08-06 南开大学 黄瓜离体叶绿体电转化方法
MA41180A (fr) 2014-12-17 2017-10-24 Bayer Cropscience Nv Plantes caractérisées par une capacité améliorée de fixation du carbone photosynthétique
WO2018129564A1 (fr) * 2017-01-09 2018-07-12 Rutgers, The State University Of New Jersey Compositions et procédés permettant d'améliorer l'efficacité de transformation de plastes dans des plantes supérieures

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR8404834A (pt) 1983-09-26 1985-08-13 Agrigenetics Res Ass Metodo para modificar geneticamente uma celula vegetal
FI864720A (fi) * 1985-11-22 1987-05-23 Ciba Geigy Ag Direkt gentransmission i plasticider och mitokondrier.
US5451513A (en) 1990-05-01 1995-09-19 The State University of New Jersey Rutgers Method for stably transforming plastids of multicellular plants
US5877402A (en) 1990-05-01 1999-03-02 Rutgers, The State University Of New Jersey DNA constructs and methods for stably transforming plastids of multicellular plants and expressing recombinant proteins therein
US5409823A (en) 1992-09-24 1995-04-25 Ciba-Geigy Corporation Methods for the production of hybrid seed
US5576198A (en) 1993-12-14 1996-11-19 Calgene, Inc. Controlled expression of transgenic constructs in plant plastids
US5545817A (en) * 1994-03-11 1996-08-13 Calgene, Inc. Enhanced expression in a plant plastid
US5545818A (en) 1994-03-11 1996-08-13 Calgene Inc. Expression of Bacillus thuringiensis cry proteins in plant plastids

Also Published As

Publication number Publication date
AU2068497A (en) 1997-09-22
CN1114692C (zh) 2003-07-16
DE69733881D1 (en) 2005-09-08
ATE301187T1 (de) 2005-08-15
EP0885298B1 (fr) 2005-08-03
AU732479B2 (en) 2001-04-26
US6376744B1 (en) 2002-04-23
DE69733881T2 (de) 2006-05-24
JP2000506019A (ja) 2000-05-23
BR9707948A (pt) 1999-07-27
WO1997032977A1 (fr) 1997-09-12
EP0885298A1 (fr) 1998-12-23
CN1222934A (zh) 1999-07-14
CA2247980A1 (fr) 1997-09-12
EP0885298A4 (fr) 2002-10-30

Similar Documents

Publication Publication Date Title
CA2247980C (fr) Transformations des plastides chez arabidopsis thaliana
Sikdar et al. Plastid transformation in Arabidopsis thaliana
Zoubenko et al. Efficient targeting of foreign genes into the tobacco plastid genome
Staub et al. Long regions of homologous DNA are incorporated into the tobacco plastid genome by transformation.
Svab et al. Stable transformation of plastids in higher plants.
US6919494B2 (en) Methods for tissue culturing and transforming elite inbreds of Zea mays L.
US5981840A (en) Methods for agrobacterium-mediated transformation
AU703838B2 (en) Nuclear-encoded transcription system in plastids of higher plants
US20140223607A1 (en) Transformation system in camelina sativa
JPH10511261A (ja) 植物色素体内でのバチルス・チューリンゲンシスｃｒｙタンパク質の発現
US20090151023A1 (en) Transformation system for Camelina sativa
JP4217482B2 (ja) 高等植物のプラスチド形質転換の方法及びベクター
Damgaard et al. Agrobacterium tumefaciens-mediated transformation of Brassica napus winter cultivars
JP2002521072A (ja) 高等植物のプラスチド中にて高レベルの蛋白を発現するための翻訳調節エレメントおよびその使用法
US20030188337A1 (en) Transgenic plants
US20060212973A1 (en) Agrobacterium-mediated transformation of turfgrass
Sarria et al. Agrobacterium-mediated transformation of Stylosanthes guianensis and production of transgenic plants
US7285700B2 (en) Plastid directed DNA constructs comprising chimeric plastid targeting regions, vectors containing same and methods of use thereof
JP2004533266A (ja) 複製ベクターに基づく色素体内での遺伝子発現
Lambe et al. Transgenic pearl millet (Pennisetum glaucum)
WO2000071704A1 (fr) Promoteur exerçant une activite specifique sur les tissus du pistil
EP1100876B1 (fr) Transformation de gazon fondee sur agrobacterium
KR20060023809A (ko) 식물의 색소체 형질전환 방법
by Transformation Long Regions of Homologous DNA Are lncorporated into the Tobacco Plastid Genome by Transformation
Chen Genetic Transformation of Perennial Ryegrass for Chemical Induced Elimination

Legal Events

Date Code Title Description
EEER Examination request
MKLA Lapsed

Effective date: 20150306