CA2240570C - Mutant chimeric dna polymerase - Google Patents

Mutant chimeric dna polymerase Download PDF

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Publication number
CA2240570C
CA2240570C CA002240570A CA2240570A CA2240570C CA 2240570 C CA2240570 C CA 2240570C CA 002240570 A CA002240570 A CA 002240570A CA 2240570 A CA2240570 A CA 2240570A CA 2240570 C CA2240570 C CA 2240570C
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CA
Canada
Prior art keywords
dna polymerase
leu
seq
glu
ala
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA002240570A
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English (en)
French (fr)
Other versions
CA2240570A1 (en
Inventor
David H. Gelfand
Fred L. Reichert
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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Publication of CA2240570A1 publication Critical patent/CA2240570A1/en
Application granted granted Critical
Publication of CA2240570C publication Critical patent/CA2240570C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA002240570A 1997-07-09 1998-07-07 Mutant chimeric dna polymerase Expired - Fee Related CA2240570C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US5206597P 1997-07-09 1997-07-09
US60/052,065 1997-07-09

Publications (2)

Publication Number Publication Date
CA2240570A1 CA2240570A1 (en) 1999-01-09
CA2240570C true CA2240570C (en) 2009-12-15

Family

ID=21975226

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002240570A Expired - Fee Related CA2240570C (en) 1997-07-09 1998-07-07 Mutant chimeric dna polymerase

Country Status (7)

Country Link
US (1) US6228628B1 (https=)
EP (1) EP0892058B1 (https=)
JP (1) JP4350815B2 (https=)
AT (1) ATE325877T1 (https=)
CA (1) CA2240570C (https=)
DE (1) DE69836779T2 (https=)
ES (1) ES2263188T3 (https=)

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US20060292578A1 (en) * 2005-06-28 2006-12-28 Weidong Zheng DNA polymerase blends and mutant DNA polymerases
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US9428737B1 (en) * 2010-01-11 2016-08-30 Eric Gaucher Taq polymerase sequences useful for incorporating analogs of nucleoside triphosphates
JP5926248B2 (ja) 2010-06-18 2016-05-25 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 増大した３’末端ミスマッチ識別能を有するｄｎａポリメラーゼ
WO2011157436A1 (en) 2010-06-18 2011-12-22 Roche Diagnostics Gmbh Dna polymerases with increased 3'-mismatch discrimination
ES2536252T3 (es) 2010-06-18 2015-05-21 F. Hoffmann-La Roche Ag ADN polimerasas con diferenciación de desapareamientos 3' aumentada
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US8759062B2 (en) 2010-06-18 2014-06-24 Roche Molecular Systems, Inc. DNA polymerases with increased 3′- mismatch discrimination
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US9567628B2 (en) 2011-06-08 2017-02-14 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
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EP2971095B1 (en) 2013-03-12 2019-11-20 Life Technologies Corporation Universal reporter-based genotyping methods, reaction mixture and kit
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EP3768832B1 (en) 2018-03-21 2023-11-29 F. Hoffmann-La Roche AG Dna polymerases for efficient and effective incorporation of methylated-dntps
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JP7209980B2 (ja) * 2020-12-11 2023-01-23 東洋紡株式会社 Ｄｎａポリメラーゼの５’→３’エキソヌクレアーゼ活性ドメインに特異的に結合する抗体
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Also Published As

Publication number Publication date
US6228628B1 (en) 2001-05-08
EP0892058B1 (en) 2006-05-10
JP4350815B2 (ja) 2009-10-21
ATE325877T1 (de) 2006-06-15
ES2263188T3 (es) 2006-12-01
CA2240570A1 (en) 1999-01-09
DE69836779D1 (de) 2007-02-15
EP0892058A3 (en) 2001-09-26
JPH1189588A (ja) 1999-04-06
DE69836779T2 (de) 2007-04-26
EP0892058A2 (en) 1999-01-20

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Effective date: 20180709