CA2210354C - Mutant luciferases - Google Patents

Mutant luciferases Download PDF

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Publication number
CA2210354C
CA2210354C CA002210354A CA2210354A CA2210354C CA 2210354 C CA2210354 C CA 2210354C CA 002210354 A CA002210354 A CA 002210354A CA 2210354 A CA2210354 A CA 2210354A CA 2210354 C CA2210354 C CA 2210354C
Authority
CA
Canada
Prior art keywords
amino acid
luciola
luciferase
protein
residue
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA002210354A
Other languages
English (en)
French (fr)
Other versions
CA2210354A1 (en
Inventor
David James Squirrell
Christopher Robin Lowe
Peter John White
James Augustus Henry Murray
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Promega Corp
Original Assignee
UK Secretary of State for Defence
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9501172.2A external-priority patent/GB9501172D0/en
Priority claimed from GBGB9508301.0A external-priority patent/GB9508301D0/en
Application filed by UK Secretary of State for Defence filed Critical UK Secretary of State for Defence
Publication of CA2210354A1 publication Critical patent/CA2210354A1/en
Application granted granted Critical
Publication of CA2210354C publication Critical patent/CA2210354C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
CA002210354A 1995-01-20 1996-01-19 Mutant luciferases Expired - Fee Related CA2210354C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GBGB9501172.2A GB9501172D0 (en) 1995-01-20 1995-01-20 Luciferases
GB9501172.2 1995-01-20
GB9508301.0 1995-04-24
GBGB9508301.0A GB9508301D0 (en) 1995-04-24 1995-04-24 Luciferases
PCT/GB1996/000099 WO1996022376A1 (en) 1995-01-20 1996-01-19 Mutant luciferases

Publications (2)

Publication Number Publication Date
CA2210354A1 CA2210354A1 (en) 1996-07-25
CA2210354C true CA2210354C (en) 2008-01-08

Family

ID=26306352

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002210354A Expired - Fee Related CA2210354C (en) 1995-01-20 1996-01-19 Mutant luciferases

Country Status (10)

Country Link
US (1) US6171808B1 (he)
EP (1) EP0804587B1 (he)
JP (1) JP4342608B2 (he)
AU (1) AU707243B2 (he)
CA (1) CA2210354C (he)
DE (1) DE69633171T2 (he)
DK (1) DK0804587T3 (he)
IN (1) IN186115B (he)
NO (1) NO322134B1 (he)
WO (1) WO1996022376A1 (he)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9707486D0 (en) * 1997-04-11 1997-05-28 Secr Defence Enzyme assays
EP1707621B1 (en) 1997-07-08 2009-11-18 Kikkoman Corporation Mutant bioluminescent protein and process for producing the mutant bioluminescent protein
DK1020524T3 (da) * 1997-07-08 2007-09-03 Kikkoman Corp Mutant bioluminescerende protein og fremgangsmåde til fremstilling af det mutante bioluminescerende protein
US6602677B1 (en) 1997-09-19 2003-08-05 Promega Corporation Thermostable luciferases and methods of production
CA2301828A1 (en) * 1997-09-19 1999-03-25 Promega Corporation Thermostable luciferases and methods of production
GB9722481D0 (en) * 1997-10-25 1997-12-24 Secr Defence Expression system
GB9823468D0 (en) 1998-10-28 1998-12-23 Secr Defence Novel enzyme
ES2292479T3 (es) * 1999-10-26 2008-03-16 The Secretary Of State For Defence Luciferasa mutante.
GB9925161D0 (en) 1999-10-26 1999-12-22 Secr Defence Novel enzyme
US7879540B1 (en) 2000-08-24 2011-02-01 Promega Corporation Synthetic nucleic acid molecule compositions and methods of preparation
GB0030727D0 (en) 2000-12-15 2001-01-31 Lumitech Uk Ltd Methods and kits for detecting kinase activity
GB0103622D0 (en) * 2001-02-14 2001-03-28 Univ Cambridge Tech Methods for detecting DNA polymerisation
CN1304426C (zh) * 2001-11-14 2007-03-14 英国国防部 信号系统及其使用的元件
US7413874B2 (en) 2002-12-09 2008-08-19 University Of Miami Nucleic acid encoding fluorescent proteins from aquatic species
GB2400659B8 (en) 2003-04-17 2007-07-09 Cambrex Bio Science Nottingham Assay methods and materials
GB0416944D0 (en) 2004-07-29 2004-09-01 Lumora Ltd Method for determining the amount of template nucleic acid present in a sample
US7728118B2 (en) 2004-09-17 2010-06-01 Promega Corporation Synthetic nucleic acid molecule compositions and methods of preparation
JP5188771B2 (ja) 2007-09-26 2013-04-24 株式会社日立製作所 変異型ホタルルシフェラーゼ
US7989190B2 (en) 2007-11-20 2011-08-02 Kikkoman Corporation Composition for analyzing nucleic acid
JP5753083B2 (ja) 2008-07-22 2015-07-22 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ Ａｄｐ検出に基づく発光ホスホトランスフェラーゼまたはａｔｐヒドロラーゼのアッセイ法
JP2011120559A (ja) 2008-12-26 2011-06-23 Kikkoman Corp ホタルルシフェラーゼ、その遺伝子、およびホタルルシフェラーゼの製造法
JP5889910B2 (ja) 2010-11-02 2016-03-22 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 新規セレンテラジン基質及び使用法
PT2635583E (pt) 2010-11-02 2015-09-11 Promega Corp Derivados da coelenterazina e métodos de utilização da mesma
US9487814B2 (en) 2013-02-22 2016-11-08 Promega Corporation Stabilized formulation for luminescent detection of luciferase and nucleoside phosphates
US9927430B2 (en) 2014-01-29 2018-03-27 Promega Corporation Pro-substrates for live cell applications
JP6703484B2 (ja) 2014-01-29 2020-06-03 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 細胞による取り込み測定のための、標識用試薬としての、キノンでマスクされたプローブ
JP6811173B2 (ja) 2014-09-11 2021-01-13 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 増強された発光を産生するために赤外発光基質を活用するルシフェラーゼ配列
JP6510041B2 (ja) 2014-10-08 2019-05-08 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 生物発光サクシネート検出アッセイ
DE102015215569A1 (de) 2015-08-14 2017-02-16 Robert Bosch Gmbh Verfahren zur Generierung eines Geheimnisses zwischen Teilnehmern eines Netzwerkes sowie dazu eingerichtete Teilnehmer des Netzwerks
WO2021162123A1 (ja) 2020-02-14 2021-08-19 キッコーマン株式会社 試料中のａｔｐ、並びにａｍｐ及び／又はａｄｐを測定するための液体組成物

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5219737A (en) 1990-03-27 1993-06-15 Kikkoman Corporation Mutant luciferase of a firefly, mutant luciferase genes, recombinant dnas containing the genes and a method of producing mutant luciferase
US5229285A (en) 1991-06-27 1993-07-20 Kikkoman Corporation Thermostable luciferase of firefly, thermostable luciferase gene of firefly, novel recombinant dna, and process for the preparation of thermostable luciferase of firefly
DE4123672A1 (de) 1991-07-17 1993-01-21 Basf Ag Siegelfaehige, peelbare kunststoffolie
EP0689587B1 (en) 1994-01-03 2008-04-09 Promega Corporation Mutant luciferases
GB9501170D0 (en) * 1994-03-23 1995-03-08 Secr Defence Luciferases

Also Published As

Publication number Publication date
US6171808B1 (en) 2001-01-09
EP0804587A1 (en) 1997-11-05
AU707243B2 (en) 1999-07-08
NO973349L (no) 1997-09-19
DE69633171T2 (de) 2005-08-18
NO973349D0 (no) 1997-07-18
WO1996022376A1 (en) 1996-07-25
DK0804587T3 (da) 2004-10-11
AU4397396A (en) 1996-08-07
EP0804587B1 (en) 2004-08-18
CA2210354A1 (en) 1996-07-25
NO322134B1 (no) 2006-08-21
IN186115B (he) 2001-06-23
DE69633171D1 (de) 2004-09-23
JPH10512750A (ja) 1998-12-08
JP4342608B2 (ja) 2009-10-14

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