CA2145750A1 - Applications de n-nucleosides fluorescents et d'analogues structuraux fluorescents de n-nucleosides - Google Patents

Applications de n-nucleosides fluorescents et d'analogues structuraux fluorescents de n-nucleosides

Info

Publication number
CA2145750A1
CA2145750A1 CA 2145750 CA2145750A CA2145750A1 CA 2145750 A1 CA2145750 A1 CA 2145750A1 CA 2145750 CA2145750 CA 2145750 CA 2145750 A CA2145750 A CA 2145750A CA 2145750 A1 CA2145750 A1 CA 2145750A1
Authority
CA
Canada
Prior art keywords
dna
probe
nucleic acid
sequence
rna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA 2145750
Other languages
English (en)
Inventor
Michael J. Conrad
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Chromagen Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2145750A1 publication Critical patent/CA2145750A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/173—Purine radicals with 2-deoxyribosyl as the saccharide radical

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

On a identifié des analogues structurels des six N-nucléosides non fluorescents que l'on trouve habituellement dans l'ARN et dans l'ADN, fluorescents de façon inhérente dans des conditions physiologiques et on a mis au point des procédés servant à les préparer. Ces analogues peuvent être incorporés dans des oligonucléotides d'ADN et/ou d'ARN par l'intermédiaire d'une synthèse enzymatique ou chimique, de manière à produire des oligonucléotides fluorescents possédant des séquences prescrites. Ces séquences analogues peuvent être identiques à des séquences d'ADN ou d'ARN gabarit ou cible, ou représenter le complément analogue desdites séquences, auxquelles peuvent s'hybrider les oligonucléotides fluorescents. L'invention concerne également des procédés de préparation de sondes d'oligonucléotides d'ARN ou d'ADN de l'invention, des intermédiaires utilisés dans lesdits procédés et des procédés d'utilisation des sondes décrites dans des méthodes d'amplification, de détection, d'identification et/ou d'hybridation d'oligonucléotides.
CA 2145750 1993-08-18 1994-08-18 Applications de n-nucleosides fluorescents et d'analogues structuraux fluorescents de n-nucleosides Abandoned CA2145750A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10845793A 1993-08-18 1993-08-18
US108,457 1993-08-18

Publications (1)

Publication Number Publication Date
CA2145750A1 true CA2145750A1 (fr) 1995-02-23

Family

ID=22322340

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2145750 Abandoned CA2145750A1 (fr) 1993-08-18 1994-08-18 Applications de n-nucleosides fluorescents et d'analogues structuraux fluorescents de n-nucleosides

Country Status (4)

Country Link
EP (1) EP0669928A1 (fr)
JP (1) JPH09505556A (fr)
CA (1) CA2145750A1 (fr)
WO (1) WO1995005391A1 (fr)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19509038A1 (de) * 1995-03-14 1996-09-19 Boehringer Mannheim Gmbh C-Nukleosid-Derivate und deren Verwendung in der Detektion von Nukleinsäuren
US5912340A (en) * 1995-10-04 1999-06-15 Epoch Pharmaceuticals, Inc. Selective binding complementary oligonucleotides
US5808035A (en) * 1995-12-08 1998-09-15 Usher; David A. Protected nucleoside and method for its synthesis
ATE275573T1 (de) * 1996-05-15 2004-09-15 Univ Rochester Neue nukleosid-analoge die polyzyklische aromatische gruppen als substituenten haben, verfahren zu ihrer herstellung und ihre anwendungen
US6218108B1 (en) * 1997-05-16 2001-04-17 Research Corporation Technologies, Inc. Nucleoside analogs with polycyclic aromatic groups attached, methods of synthesis and uses therefor
US6289229B1 (en) 1998-01-20 2001-09-11 Scimed Life Systems, Inc. Readable probe array for in vivo use
ES2294862T5 (es) 1998-11-09 2011-04-12 Eiken Kagaku Kabushiki Kaisha Procedimiento de síntesis de ácido nucleico.
US8017357B2 (en) 2000-04-07 2011-09-13 Eiken Kagaku Kabushiki Kaisha Method of amplifying nucleic acid by using double-stranded nucleic acid as template
GB0016836D0 (en) * 2000-07-07 2000-08-30 Lee Helen Improved dipstick assays (1)
JP2004313181A (ja) 2003-04-02 2004-11-11 Canon Inc 感染症起炎菌検出用プローブ及びプローブセット、ならびに担体及び遺伝子検査方法
WO2019051732A1 (fr) 2017-09-14 2019-03-21 中科芯瑞(苏州)生物科技有限公司 Procédé et kit de synthèse d'acide nucléique dans des conditions de température constantes
EP4105327A4 (fr) 2021-04-29 2023-01-18 Ningbo Institute of Life and Health Industry University of Chinese Academy of Sciences Procédé de synthèse d'acide nucléique dans des conditions de température constante, kit et application

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3960840A (en) * 1972-12-29 1976-06-01 University Of Illinois Foundaton Fluorescent derivatives of adenine-containing compounds
WO1987001373A1 (fr) * 1985-09-09 1987-03-12 Teijin Limited Derives de nucleotides de pyridopyrimidine
JPS6259293A (ja) * 1985-09-09 1987-03-14 Teijin Ltd 螢光性ヌクレオシド又はヌクレオチド
JPS62255499A (ja) * 1986-04-28 1987-11-07 Teijin Ltd 螢光性ヌクレオシド又はヌクレオチド
JPH07504087A (ja) * 1992-02-12 1995-05-11 クロマジェン　インク 蛍光性ｎ−ヌクレオシド及び蛍光性ｎ−ヌクレオシド構造類似体の応用

Also Published As

Publication number Publication date
JPH09505556A (ja) 1997-06-03
EP0669928A1 (fr) 1995-09-06
WO1995005391A1 (fr) 1995-02-23

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Legal Events

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