CA2090701A1 - Solubilisation de proteines sous des formes actives - Google Patents
Solubilisation de proteines sous des formes activesInfo
- Publication number
- CA2090701A1 CA2090701A1 CA2090701A CA2090701A CA2090701A1 CA 2090701 A1 CA2090701 A1 CA 2090701A1 CA 2090701 A CA2090701 A CA 2090701A CA 2090701 A CA2090701 A CA 2090701A CA 2090701 A1 CA2090701 A1 CA 2090701A1
- Authority
- CA
- Canada
- Prior art keywords
- protein
- solubilization
- proteins
- active forms
- refolding
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000004169 proteins and genes Human genes 0.000 title abstract 4
- 108090000623 proteins and genes Proteins 0.000 title abstract 4
- 230000007928 solubilization Effects 0.000 title 1
- 238000005063 solubilization Methods 0.000 title 1
- YTPLMLYBLZKORZ-UHFFFAOYSA-N Divinylene sulfide Natural products C=1C=CSC=1 YTPLMLYBLZKORZ-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
- C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
- C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Endocrinology (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Toxicology (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Procédé de préparation d'une protéine sous une forme physiologiquement active, ledit procédé comprenant l'utilisation d'une source de protéine sous une forme au moins partiellement solubilisée; et d'un agent de réplication comprenant une quantité efficace d'un monothiol de faible masse moléculaire ou d'un dérivé de ce dernier. Dans ce procédé, on soumet la protéine solubilisée à une étape de réplication, en présence de l'agent de réplication.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AUPK2159 | 1990-09-05 | ||
AUPK215990 | 1990-09-05 | ||
PCT/AU1991/000395 WO1992004382A1 (fr) | 1990-09-05 | 1991-08-29 | Solubilisation de proteines sous des formes actives |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2090701A1 true CA2090701A1 (fr) | 1992-03-10 |
CA2090701C CA2090701C (fr) | 2000-05-16 |
Family
ID=3774938
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002090701A Expired - Fee Related CA2090701C (fr) | 1990-09-05 | 1991-08-29 | Solubilisation de proteines sous des formes actives |
Country Status (8)
Country | Link |
---|---|
EP (1) | EP0547102B1 (fr) |
AT (1) | ATE168115T1 (fr) |
AU (1) | AU664021B2 (fr) |
CA (1) | CA2090701C (fr) |
DE (1) | DE69129747T2 (fr) |
DK (1) | DK0547102T3 (fr) |
ES (1) | ES2119779T3 (fr) |
WO (1) | WO1992004382A1 (fr) |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU4665193A (en) * | 1992-07-02 | 1994-01-31 | Pitman-Moore, Inc. | A process for recovering a recombinant protein, in biologically active form, from a solution containing inactive protein |
DK44593D0 (da) * | 1993-04-20 | 1993-04-20 | Novo Nordisk As | Fremgangsmaade til fremstilling af et polypeptid |
CN1057775C (zh) * | 1993-09-22 | 2000-10-25 | 味之素株式会社 | 具有抗血栓活性的肽及其制法 |
ZA955789B (en) * | 1994-07-15 | 1996-03-11 | Novo Nordisk As | A method of converting a hydrophobic derivative of a polypeptide into the native form |
DE10309691B4 (de) * | 2003-02-28 | 2018-10-04 | Hans-Knöll-Institut für Naturstoff-Forschung e.V. | Gewinnung von löslichen Proteinen aus der Biomasse rekombinanter Mikroorganismen |
BRPI1011940B8 (pt) | 2009-06-22 | 2021-08-03 | Amgen Inc | método de redobramento de uma proteína expressa em um sistema de expressão de não mamífero |
EP2445924B2 (fr) | 2009-06-25 | 2023-12-13 | Amgen Inc. | Procédés de purification par capture de protéines exprimées dans un système non mammifère |
EP3617220B1 (fr) | 2013-02-12 | 2021-03-24 | Bristol-Myers Squibb Company | Procédés de repliement de protéine à ph élevé |
EP2956468B1 (fr) | 2013-02-12 | 2020-06-10 | Bristol-Myers Squibb Company | Procédés de repliement de protéine utilisant la filtration tangentielle |
EP2978770B1 (fr) | 2013-03-29 | 2019-11-06 | Dr. Reddy's Laboratories Limited | Repliement de protéines |
US10745457B2 (en) | 2015-09-02 | 2020-08-18 | Merck Sharp & Dohme Corp. | Process for obtaining insulin with correctly formed disulfide bonds |
Family Cites Families (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS6079000A (ja) * | 1983-10-04 | 1985-05-04 | Takeda Chem Ind Ltd | ヒトγ型インタ−フエロン単量体の製造法 |
ATE92935T1 (de) * | 1984-12-06 | 1993-08-15 | Synergen Biolog Inc | Serinproteaseinhibitoren und isolierungsverfahren dazu. |
DE3537708A1 (de) * | 1985-10-23 | 1987-04-23 | Boehringer Mannheim Gmbh | Verfahren zur aktivierung von t-pa nach expression in prokaryonten |
US4766205A (en) * | 1985-11-13 | 1988-08-23 | Beatrice Companies, Inc. | Method for isolation of recombinant polypeptides in biologically active forms |
DE3611817A1 (de) * | 1986-04-08 | 1987-10-15 | Boehringer Mannheim Gmbh | Verfahren zur renaturierung von proteinen |
AU612133B2 (en) * | 1987-02-20 | 1991-07-04 | Natinco Nv | Production of proteins in active forms |
IL86090A (en) * | 1987-04-16 | 1993-03-15 | Cetus Oncology Corp | Production of purified, biologically active, bacterially produced recombinant human csf-1 |
EP0295859B1 (fr) * | 1987-06-15 | 1994-11-17 | Southern Cross Biotech Pty.Ltd. | Préparation de protéines dans les formes actives |
WO1989001046A1 (fr) * | 1987-07-29 | 1989-02-09 | Schering Biotech Corporation | Purification d'interleukine-4 humaines exprimee dans escherichia coli |
CA1340586C (fr) * | 1988-09-23 | 1999-06-08 | Cetus Corporation | Methode pour recuperer l'interferon beta produit par voie microbienne |
DE3832898A1 (de) * | 1988-09-28 | 1990-04-12 | Boehringer Mannheim Gmbh | Praeparat von in prokaryonten exprimiertem plasminogenaktivator |
DE3901719A1 (de) * | 1989-01-21 | 1990-07-26 | Hoechst Ag | Verfahren zur herstellung eines insulinvorlaeufers |
DE3901718A1 (de) * | 1989-01-21 | 1990-07-26 | Hoechst Ag | Verfahren zur renaturierung falscher rekombinanten von insulinvorlaeufern |
GB8927546D0 (en) * | 1989-12-06 | 1990-02-07 | Ciba Geigy | Process for the production of biologically active tgf-beta |
US5235043A (en) * | 1990-04-06 | 1993-08-10 | Synergen, Inc. | Production of biologically active, recombinant members of the ngf/bdnf family of neurotrophic proteins |
DE4113750A1 (de) * | 1991-04-26 | 1992-10-29 | Boehringer Mannheim Gmbh | Verbesserung der renaturierung bei der sekretion von disulfidverbrueckten proteinen |
-
1991
- 1991-08-29 AU AU84035/91A patent/AU664021B2/en not_active Ceased
- 1991-08-29 AT AT91915661T patent/ATE168115T1/de not_active IP Right Cessation
- 1991-08-29 CA CA002090701A patent/CA2090701C/fr not_active Expired - Fee Related
- 1991-08-29 DE DE69129747T patent/DE69129747T2/de not_active Expired - Fee Related
- 1991-08-29 DK DK91915661T patent/DK0547102T3/da active
- 1991-08-29 EP EP91915661A patent/EP0547102B1/fr not_active Expired - Lifetime
- 1991-08-29 ES ES91915661T patent/ES2119779T3/es not_active Expired - Lifetime
- 1991-08-29 WO PCT/AU1991/000395 patent/WO1992004382A1/fr active IP Right Grant
Also Published As
Publication number | Publication date |
---|---|
CA2090701C (fr) | 2000-05-16 |
DK0547102T3 (da) | 1999-04-19 |
EP0547102B1 (fr) | 1998-07-08 |
EP0547102A4 (fr) | 1994-04-27 |
DE69129747D1 (de) | 1998-08-13 |
AU664021B2 (en) | 1995-11-02 |
ATE168115T1 (de) | 1998-07-15 |
WO1992004382A1 (fr) | 1992-03-19 |
ES2119779T3 (es) | 1998-10-16 |
DE69129747T2 (de) | 1998-11-12 |
EP0547102A1 (fr) | 1993-06-23 |
AU8403591A (en) | 1992-03-30 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKLA | Lapsed |