CA2087730C - Use of crosslinked crystals as a novel form of enzyme immobilization - Google Patents

Use of crosslinked crystals as a novel form of enzyme immobilization

Info

Publication number
CA2087730C
CA2087730C CA002087730A CA2087730A CA2087730C CA 2087730 C CA2087730 C CA 2087730C CA 002087730 A CA002087730 A CA 002087730A CA 2087730 A CA2087730 A CA 2087730A CA 2087730 C CA2087730 C CA 2087730C
Authority
CA
Canada
Prior art keywords
crosslinked
crystal
enzyme
protein
thermolysin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA002087730A
Other languages
English (en)
French (fr)
Other versions
CA2087730A1 (en
Inventor
Manuel A. Navia
Nancy L. St. Clair
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Vertex Pharmaceuticals Inc
Original Assignee
Vertex Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Vertex Pharmaceuticals Inc filed Critical Vertex Pharmaceuticals Inc
Publication of CA2087730A1 publication Critical patent/CA2087730A1/en
Application granted granted Critical
Publication of CA2087730C publication Critical patent/CA2087730C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/06—Dipeptides
    • C07K5/06104—Dipeptides with the first amino acid being acidic
    • C07K5/06113—Asp- or Asn-amino acid
    • C07K5/06121—Asp- or Asn-amino acid the second amino acid being aromatic or cycloaliphatic
    • C07K5/0613—Aspartame
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2462—Lysozyme (3.2.1.17)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6448—Elastases, e.g. pancreatic elastase (3.4.21.36); leukocyte elastase (3.4.31.37)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C12N9/82—Asparaginase (3.5.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/002—Electrode membranes
    • C12Q1/003—Functionalisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Liquid Crystal Substances (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Crystals, And After-Treatments Of Crystals (AREA)
  • Piezo-Electric Or Mechanical Vibrators, Or Delay Or Filter Circuits (AREA)
CA002087730A 1990-08-03 1991-07-30 Use of crosslinked crystals as a novel form of enzyme immobilization Expired - Lifetime CA2087730C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US56228090A 1990-08-03 1990-08-03
US562,280 1990-08-03

Publications (2)

Publication Number Publication Date
CA2087730A1 CA2087730A1 (en) 1992-02-04
CA2087730C true CA2087730C (en) 1999-07-20

Family

ID=24245600

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002087730A Expired - Lifetime CA2087730C (en) 1990-08-03 1991-07-30 Use of crosslinked crystals as a novel form of enzyme immobilization

Country Status (26)

Country Link
EP (2) EP0861888B1 (ro)
JP (2) JPH05508549A (ro)
AT (2) ATE240391T1 (ro)
AU (1) AU662104B2 (ro)
BR (1) BR9106732A (ro)
CA (1) CA2087730C (ro)
CZ (1) CZ12393A3 (ro)
DE (2) DE69133521T2 (ro)
DK (2) DK0861888T3 (ro)
ES (2) ES2199933T3 (ro)
FI (1) FI120099B (ro)
HK (2) HK1023362A1 (ro)
HU (2) HUT65904A (ro)
IE (1) IE912767A1 (ro)
IL (1) IL99046A (ro)
LT (1) LT3366B (ro)
LV (1) LV10304B (ro)
MD (1) MD1592B2 (ro)
NO (1) NO930360L (ro)
PT (1) PT98564B (ro)
RU (1) RU2124052C1 (ro)
SG (1) SG70966A1 (ro)
SK (1) SK283185B6 (ro)
TJ (1) TJ210R3 (ro)
UA (1) UA27035C2 (ro)
WO (1) WO1992002617A1 (ro)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5932212A (en) * 1996-05-24 1999-08-03 Altus Biologics, Inc. Crosslinked protein crystal formulations and their use as catalysts in organic solvents
WO1998013119A1 (en) * 1996-09-24 1998-04-02 Altus Biologics Inc. Crosslinked protein crystals as universal separation media
US6140475A (en) * 1997-04-11 2000-10-31 Altus Biologics Inc. Controlled dissolution crosslinked protein crystals
US6500933B1 (en) 1997-09-05 2002-12-31 Altus Biologics Inc. Methods of preparing carbohydrate crosslinked glycoprotein crystals
ES2306477T3 (es) * 1997-09-05 2008-11-01 Altus Pharmaceuticals Inc. Cristales de glicoproteina reticulados por carbohidratos.
US6979561B1 (en) 1998-10-09 2005-12-27 Gilead Sciences, Inc. Non-homogeneous systems for the resolution of enantiomeric mixtures
GB9823334D0 (en) 1998-10-23 1998-12-23 Darwin Discovery Ltd Enzyme coupling
EP1372703A2 (en) * 2001-02-16 2004-01-02 Ibex Technologies Matrix stabilized enzyme crystals and methods of use
US7241272B2 (en) 2001-11-13 2007-07-10 Baxter International Inc. Method and composition for removing uremic toxins in dialysis processes
US20090035828A1 (en) * 2004-01-01 2009-02-05 Tholath Emilia Abraham Method For the Preparation of Cross Linked Protein Crystals
PT1729797E (pt) 2004-03-22 2008-12-17 Solvay Pharm Gmbh Composições farmacêuticas orais de produtos contendo lipase, em particular de pancreatina, contendo tensioactivos
EP1809320B1 (en) 2004-10-14 2010-07-21 Altus Pharmaceuticals Inc. Compositions containing lipase; protease and amylase for treating pancreatic insufficiency
EP1913138B1 (en) 2005-07-29 2016-08-24 Abbott Laboratories GmbH Processes for the manufacture of pancreatin powder with low virus content
US11266607B2 (en) 2005-08-15 2022-03-08 AbbVie Pharmaceuticals GmbH Process for the manufacture and use of pancreatin micropellet cores
US9198871B2 (en) 2005-08-15 2015-12-01 Abbott Products Gmbh Delayed release pancreatin compositions
US10072256B2 (en) 2006-05-22 2018-09-11 Abbott Products Gmbh Process for separating and determining the viral load in a pancreatin sample
JP5248676B2 (ja) 2009-05-22 2013-07-31 サンヨー食品株式会社 不斉酸化反応を有する蛋白質複合体およびその製造方法
CN102753570B (zh) * 2009-09-15 2016-01-20 阿尔泰亚科技公司 A蛋白晶体和交联晶体及其使用方法
US9303255B2 (en) 2010-08-26 2016-04-05 Aisin Seiki Kabushiki Kaisha Electrode having enzyme crystals immobilized thereon, method for producing electrode having enzyme crystals immobilized thereon, and biological fuel cell and biosensor provided with electrode having enzyme crystals immobilized thereon
EP3003358B1 (en) 2013-06-07 2021-03-24 Allena Pharmaceuticals Inc. Compositions and devices for dialysis
US20180028454A1 (en) 2015-02-04 2018-02-01 Abbvie Inc. Pharmaceutical compositions and methods of use thereof to treat pancreatic enzyme insufficiency
ES2962985T3 (es) * 2017-06-15 2024-03-22 Univ Chicago Composiciones para el tratamiento del cáncer
WO2023194615A1 (en) * 2022-04-07 2023-10-12 Need4Health B.V. Method for determining insulin dosage

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3664926A (en) 1970-02-26 1972-05-23 Merck & Co Inc Crystallization of l-asparaginase
DE2857828C2 (ro) 1977-01-27 1990-06-07 Toyo Soda Mfg. Co., Ltd., Shinnanyo, Yamaguchi, Jp
US4373023A (en) * 1980-10-14 1983-02-08 Massachusetts Institute Of Technology Process for neutralizing heparin
EP0367302A3 (en) * 1982-04-23 1991-06-19 Wako Pure Chemical Industries, Ltd. Process for semi-synthesis of human insulin, water-soluble cross-linked achromobacter protease i for use therein and a process for preparing the same
US4411996A (en) * 1982-06-30 1983-10-25 Nabisco Brands, Inc. Process for isomerizing glucose
WO1985003247A1 (en) * 1984-01-20 1985-08-01 Eye Products Limited Partnership Method for cleaning contact lenses
FR2566798B1 (fr) * 1984-06-27 1986-12-12 Centre Nat Rech Scient Membrane porteuse de la luciferase pour le dosage de l'atp et son procede de production
US4650758A (en) * 1984-09-21 1987-03-17 Cetus Corporation Stabilization of enzymes useful in the production of glucosone and other enzymatic processes
JPS6317691A (ja) * 1986-07-09 1988-01-25 Agency Of Ind Science & Technol アスパルテ−ム製造用固定化酵素
FI85285C (fi) * 1988-05-13 1992-03-25 Stabra Ag Tvaerbundet, vattenoloesligt glukosisomeras och foerfarande foer framstaellning daerav.

Also Published As

Publication number Publication date
UA27035C2 (uk) 2000-02-28
HK1012411A1 (en) 1999-07-30
FI930454A0 (fi) 1993-02-02
ATE323158T1 (de) 2006-04-15
DE69133258T2 (de) 2004-03-18
ATE240391T1 (de) 2003-05-15
IL99046A0 (en) 1992-07-15
HUT65904A (en) 1994-07-28
TJ210R3 (en) 1998-11-14
LV10304B (en) 1995-06-20
ES2199933T3 (es) 2004-03-01
ES2263187T3 (es) 2006-12-01
IE912767A1 (en) 1992-02-12
HK1023362A1 (en) 2000-09-08
CZ12393A3 (en) 1993-10-13
SK283185B6 (sk) 2003-03-04
RU2124052C1 (ru) 1998-12-27
PT98564B (pt) 1999-01-29
LT3366B (en) 1995-07-25
LTIP1077A (en) 1995-04-25
EP0550450B1 (en) 2003-05-14
DE69133521D1 (de) 2006-05-24
FI930454A (fi) 1993-02-02
AU662104B2 (en) 1995-08-24
AU8315491A (en) 1992-03-02
EP0550450A1 (en) 1993-07-14
CA2087730A1 (en) 1992-02-04
DE69133521T2 (de) 2006-12-07
EP0861888B1 (en) 2006-04-12
DE69133258D1 (de) 2003-06-18
WO1992002617A1 (en) 1992-02-20
JP2004000230A (ja) 2004-01-08
NO930360L (no) 1993-04-02
HU211521A9 (en) 1995-11-28
MD1592B2 (ro) 2001-01-31
JPH05508549A (ja) 1993-12-02
SK5993A3 (en) 1993-07-07
BR9106732A (pt) 1993-08-17
LV10304A (lv) 1994-10-20
IL99046A (en) 1998-09-24
HU9300275D0 (en) 1993-04-28
EP0861888A1 (en) 1998-09-02
IE20040113A1 (en) 2004-05-05
DK0550450T3 (da) 2003-09-08
PT98564A (pt) 1992-06-30
NO930360D0 (no) 1993-02-02
FI120099B (fi) 2009-06-30
SG70966A1 (en) 2000-03-21
DK0861888T3 (da) 2006-08-14

Similar Documents

Publication Publication Date Title
CA2087730C (en) Use of crosslinked crystals as a novel form of enzyme immobilization
US5618710A (en) Crosslinked enzyme crystals
IE83874B1 (en) Use of crosslinked crystals as a novel form of enzyme immobilization
US3907644A (en) Creatinine amidohydrolase composition and process for the determination of creatinine
Bednarski et al. Membrane-enclosed enzymic catalysis (MEEC): a useful, practical new method for the manipulation of enzymes in organic synthesis
US5801022A (en) Method of producing a product with crosslinked crystals of thermolysin
Marconi Immobilized enzymes: their catalytic behaviour and their industrial and analytical applications
Everse et al. Immobilized enzymes in biochemical analysis
Rai et al. Production of D-amino acids using immobilized D-hydantoinase from lentil, Lens esculenta, seeds
NZ264042A (en) Crosslinked crystalline immobilized protein, biosensor containing the crosslinked protein crystal
IE85134B1 (en) Use of Crosslinked Crystals as a Novel Form of Enzyme Immobilization
EP0367302A2 (en) Process for semi-synthesis of human insulin, water-soluble cross-linked Achromobacter protease I for use therein and a process for preparing the same
ES2323572T3 (es) Metodo de preparacion de agregados enzimaticos reticulados.
JPS6214272B2 (ro)
Hagiwara et al. Fibrin membrane endowed with biological function VII. An approach to an artificial liver; Conversion of ammonia to urea invitro
JPS5995886A (ja) Ｌ−トリプトフアンアミノペプチダ−ゼ及びその製造方法
LÁSZLÓ SUPERVISOR: MÁRIA L. SIMON
Limhardt et al. Patents and literature
JPH0428353B2 (ro)

Legal Events

Date Code Title Description
EEER Examination request
MKEX Expiry