CA2048696A1 - Plant chitinase gene and use thereof - Google Patents

Plant chitinase gene and use thereof

Info

Publication number
CA2048696A1
CA2048696A1 CA002048696A CA2048696A CA2048696A1 CA 2048696 A1 CA2048696 A1 CA 2048696A1 CA 002048696 A CA002048696 A CA 002048696A CA 2048696 A CA2048696 A CA 2048696A CA 2048696 A1 CA2048696 A1 CA 2048696A1
Authority
CA
Canada
Prior art keywords
chitinase
dna sequence
sequence
plant
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002048696A
Other languages
English (en)
French (fr)
Inventor
Jorn D. Mikkelsen
Kirsten Bojsen
Klaus K. Nielsen
Lars Berglund
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sandoz AG
DuPont Nutrition Biosciences ApS
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2048696A1 publication Critical patent/CA2048696A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50Isolated enzymes; Isolated proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2434Glucanases acting on beta-1,4-glucosidic bonds
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2434Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2442Chitinase (3.2.1.14)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01014Chitinase (3.2.1.14)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01039Glucan endo-1,3-beta-D-glucosidase (3.2.1.39)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01058Glucan 1,3-beta-glucosidase (3.2.1.58)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Pest Control & Pesticides (AREA)
  • Environmental Sciences (AREA)
  • Dentistry (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Cosmetics (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
CA002048696A 1991-04-08 1991-08-06 Plant chitinase gene and use thereof Abandoned CA2048696A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK91616A DK61691D0 (da) 1991-04-08 1991-04-08 Genetiske konstruktioner
DK616/91 1991-04-08

Publications (1)

Publication Number Publication Date
CA2048696A1 true CA2048696A1 (en) 1992-10-09

Family

ID=8095589

Family Applications (2)

Application Number Title Priority Date Filing Date
CA002048696A Abandoned CA2048696A1 (en) 1991-04-08 1991-08-06 Plant chitinase gene and use thereof
CA002048477A Abandoned CA2048477A1 (en) 1991-04-08 1991-08-08 Plant chitinase gene and use thereof

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA002048477A Abandoned CA2048477A1 (en) 1991-04-08 1991-08-08 Plant chitinase gene and use thereof

Country Status (11)

Country Link
EP (1) EP0579709A1 (ja)
JP (1) JPH06507070A (ja)
AU (1) AU659455B2 (ja)
CA (2) CA2048696A1 (ja)
CZ (1) CZ209293A3 (ja)
DK (1) DK61691D0 (ja)
HU (1) HUT67059A (ja)
IE (1) IE921104A1 (ja)
NZ (1) NZ242270A (ja)
SK (1) SK108193A3 (ja)
WO (1) WO1992017591A1 (ja)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2145984A1 (en) * 1992-10-05 1994-04-14 Leo Sjoerd Melchers Antifungal chitin binding proteins and dna coding therefor
DE4234131C2 (de) * 1992-10-09 1995-08-24 Max Planck Gesellschaft Transgener pathogen-resistenter Organismus
USRE39238E1 (en) 1992-10-09 2006-08-15 Max-Planck-Gesellschaft zür Forderung der Wissenschaften e.V. Transgenic pathogen-resistant organism
GB9303725D0 (en) * 1993-02-24 1993-04-14 Sandoz Ltd Improvements in or relating to organic compounds
US5530187A (en) * 1993-07-16 1996-06-25 The Salk Institute For Biological Studies Transgenic plants containing multiple disease resistance genes
GB9321714D0 (en) * 1993-10-21 1993-12-15 Sandoz Ltd Improvements in or relating to organic compounds
PL327499A1 (en) * 1995-11-03 1998-12-21 Unilever Nv Composition of natural origin for fighting against fungi
GB9526238D0 (en) 1995-12-21 1996-02-21 Sandoz Ltd Improvements in or relating to organic compounds
US5773696A (en) 1996-03-29 1998-06-30 Monsanto Company Antifungal polypeptide and methods for controlling plant pathogenic fungi
US6121436A (en) * 1996-12-13 2000-09-19 Monsanto Company Antifungal polypeptide and methods for controlling plant pathogenic fungi
GB9804368D0 (en) * 1998-03-03 1998-04-22 Agrevo Uk Ltd Transgenics & fungicides
US6114603A (en) * 1998-03-27 2000-09-05 John Innes Center Genetic engineering of sugarbeet plants
US6489542B1 (en) 1998-11-04 2002-12-03 Monsanto Technology Llc Methods for transforming plants to express Cry2Ab δ-endotoxins targeted to the plastids
WO2002061043A2 (en) * 2001-01-29 2002-08-08 Cargill, Incorporated Fungal resistant transgenic plants
DE102008014041A1 (de) 2008-03-13 2009-09-17 Leibniz-Institut für Pflanzengenetik Und Kulturpflanzenforschung (IPK) Verfahren zur Erzeugung einer Breitband-Resistenz gegenüber Pilzen in transgenen Pflanzen
EP3058826A1 (en) 2011-09-08 2016-08-24 Novozymes BioAG A/S Seed treatment methods and compositions
CN104093680B (zh) 2011-09-14 2016-08-24 诺维信生物农业公司 组合使用脂壳寡糖和/或壳寡糖与解磷微生物促植物生长
AU2012312003B2 (en) 2011-09-23 2015-10-22 Novozymes Bioag A/S Chitooligosaccharides and methods for use in enhancing plant growth
MX2014003411A (es) 2011-09-23 2015-03-19 Novozymes Bioag As Quitooligosacaridos y metodos para el uso en el mejoramiento del crecimiento del maiz.
IN2014CN02912A (ja) 2011-09-23 2015-07-03 Novozymes Bioag As
EP2747567B1 (en) 2011-09-23 2016-11-16 Novozymes Bioag A/S Chitooligosaccharides and methods for use in enhancing soybean growth
WO2013050611A1 (en) 2011-10-07 2013-04-11 Basf Plant Science Company Gmbh Method of producing plants having increased resistance to pathogens
WO2013050318A1 (en) 2011-10-07 2013-04-11 Basf Plant Science Company Gmbh Method of producing plants having increased resistance to pathogens
WO2013050593A1 (en) 2011-10-07 2013-04-11 Basf Plant Science Company Gmbh Method of producing plants having increased resistance to pathogens
WO2013053686A1 (en) 2011-10-10 2013-04-18 Basf Plant Science Company Gmbh Method of producing plants having increased resistance to pathogens
WO2013053711A1 (en) 2011-10-10 2013-04-18 Basf Plant Science Company Gmbh Method of producing plants having increased resistance to pathogens
WO2016130020A1 (en) 2015-02-13 2016-08-18 Bioforsk - Norwegian Institute For Agricultural And Environmental Research Plant resistance genes
CA3116832A1 (en) * 2018-10-19 2020-04-23 Universiteit Antwerpen Anti-pathogenic activity of a bifunctional peptidoglycan/chitin hydrolase

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR910700346A (ko) * 1988-12-16 1991-03-14 제임스 제이. 플라인 트랜스제닉(transgenic)식물내에서 키티나제를 과발현시키는 방법
DE69034081T2 (de) * 1989-03-24 2004-02-12 Syngenta Participations Ag Krankheitsresistente transgene Pflanze
IL97020A (en) * 1990-01-30 2000-12-06 Mogen Int Recombinant polynucleotides comprising a chitinase gene and a glucanase gene

Also Published As

Publication number Publication date
CA2048477A1 (en) 1992-10-09
HU9302829D0 (en) 1994-01-28
EP0579709A1 (en) 1994-01-26
WO1992017591A1 (en) 1992-10-15
SK108193A3 (en) 1994-04-06
AU1659992A (en) 1992-11-02
CZ209293A3 (en) 1994-04-13
IE921104A1 (en) 1992-10-21
JPH06507070A (ja) 1994-08-11
DK61691D0 (da) 1991-04-08
NZ242270A (en) 1994-07-26
AU659455B2 (en) 1995-05-18
HUT67059A (en) 1995-01-30

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Legal Events

Date Code Title Description
FZDE Discontinued