KR910700346A - 트랜스제닉(transgenic)식물내에서 키티나제를 과발현시키는 방법 - Google Patents

트랜스제닉(transgenic)식물내에서 키티나제를 과발현시키는 방법 Download PDF

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KR910700346A
KR910700346A KR1019900701797A KR900701797A KR910700346A KR 910700346 A KR910700346 A KR 910700346A KR 1019900701797 A KR1019900701797 A KR 1019900701797A KR 900701797 A KR900701797 A KR 900701797A KR 910700346 A KR910700346 A KR 910700346A
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recombinant dna
dna construct
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엘리자베스 브로글리 커렌
마틴 브로글리 리챠드
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제임스 제이. 플라인
이. 아이. 듀 퐁 드 네모아 앤드 캄파니
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
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    • C12N15/09—Recombinant DNA-technology
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2442—Chitinase (3.2.1.14)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01014—Chitinase (3.2.1.14)

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Abstract

내용 없음.

Description

트랜스제닉(transgenic)식물내에서 키티나제를 과발현시키는 방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 콩 제놈성 클론 λ초5B 및 콩 키타나제 유전자를 함유하는 4.7kb Hind III-EcoRI 단편의 제한지도이다.
제2도는 콩 키티나제 유전자를 함유하는 4.7kb Hind III-EcoRI 단편의 뉴클레오타이드 서열도이다.
제3도는 pK35 CHN의 작제에 포함된 단계의 요약도이다.

Claims (26)

  1. 식물 키티나제 유전자(b)에 작동적으로 연결되어 식물 키티나제 폴리펩타이드의 과발현을 유도함으로써 식물 병원성 진균에 대한 내성을 부여하는 고수준 프로모터(a)를 함유함을 특징으로 하는, 식물을 형질전환시킬 수 있는 재조합 DNA 작제물.
  2. 제1항에 있어서, 고수준 프로모터가 바이러스 제놈으로부터 유도된 재조합 DNA 작제물.
  3. 제1항에 있어서, 고수준 프로모터가 아그로박테리움(Agrobacterium)의 오파인 신타제 유전자로부터 유도된 재조합 DNA 작제물.
  4. 제1항에 있어서, 고수준 프로모터가 콜리플라우어 모자이크 바이러스의 35S 및 19S 성분, 아그로박테리움의 오파인 신티제 유전자의 NOS 및 OCS 프로모터, RUBISCO 소형 아단위의 프로모터 및 클로로필 A/B 결합 단백질 유전자의 프로모터로 이루어진 그룹중에서 선택된 재조합 DNA 작제물.
  5. 제1항에 있어서, 고수준 프로모터가 또한 전사 및 발현을 더욱 증가시키기 위한 인핸서도 함유하는 재조합 DNA 작제물.
  6. 제5항에 있어서, 인핸서가 바이러스 제놈으로부터 재조합 DNA 작제물.
  7. 제1항에 있어서, 인핸서가 콜리플라우어 모자이크 바이러스의 35S 프로모터로부터 유도된 재조합 DNA 작제물.
  8. 제5항에 있어서, 인핸서가 아그로박테리움의 오파인 신타제 유전자로부터 유도된 재조합 DNA 작제물.
  9. 제1항에 있어서, 고수준 프로모터가 조직 특이성 프로모터인 재조합 DNA 작제물.
  10. 제9항에 있어서, 조직특이성 프로모터가 뿌리 특이성인 재조합 DNA 작제물.
  11. 제9항에 있어서, 조직특이성 프로모터가 잎 특이성인 재조합 DNA 작제물.
  12. 제9항에 있어서, 조직특이성 프로모터가 줄기 특이성인 재조합 DNA 작제물.
  13. 제9항에 있어서, 조직특이성 프로모터가 종자 특이성인 재조합 DNA 작제물.
  14. 제9항에 있어서, 조직특이성 프로모터가 꽃잎 특이성인 재조합 DNA 작제물.
  15. 제1항에 있어서, 고수준 프로모터가 콜리플라우어 모자이크 바이러스의 35S성분이고 식물 키티나제 유전자가 콩에서 유도된 재조합 DNA 작제물.
  16. 제1항에 있어서, pK35CHN641 및 pK35CHN695로 이루어진 그룹중에서 선택된 플라스미드를 함유하는 재조합 DNA 작제물.
  17. 제1항 내지 16항중 어느 한 항에 따른 재조합 DNA 작제물을 함유하는 트랜스제닉(transgenic)식물.
  18. 식물이 옥수수, 알팔파, 귀리, 기장, 밀, 벼, 보리 및 사탕수수로 이루어진 그룹중에서 선택된 단자엽 식물인 제1항에 따른 재조합 DNA 작제물을 함유하는 트랜스제닉 식물.
  19. 식물이 대두, 담배, 페튜니아, 목화, 사탕무우, 해바라기, 당근, 셀러리, 아마, 양배추, 오이, 후추, 토마토, 감자, 브라시카, 콩, 딸기 및 상치로 이루어진 그룹중에서 선택된 상가엽 식물인 제1항에 따른 재조합 DNA 작제물을 함유하는 트랜스제닉 식물.
  20. 제15항에 다른 재조합 DNA 작제물을 함유하는 트랜스제닉 담배.
  21. 제15항에 다른 재조합 DNA 작제물을 함유하는 트랜스제닉 벼.
  22. 제15항에 따른 재조합 DNA 작제물을 함유하는 트랜스제닉 캐놀라.
  23. 제15항에 따른 재조합 DNA 작제물을 함유하는 트랜스제닉 토마토.
  24. 제17항 내지 23항중 어느 한 항에 따른 트랜스제닉 식물을 재배하여 수득한 종자.
  25. 제1항에 있어서, 식물 병원성 진균이 토양 병원균인 재조합 DNA 작제물.
  26. 제20항 내지 23항중 어느 한 항에 있어서, 식물이 토양 병원성 진균에 내성이 있는 트랜스제닉 식물.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019900701797A 1988-12-16 1989-12-13 트랜스제닉(transgenic)식물내에서 키티나제를 과발현시키는 방법 KR910700346A (ko)

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Application Number Priority Date Filing Date Title
US28525288A 1988-12-16 1988-12-16
US285252 1988-12-16
PCT/US1989/005501 WO1990007001A1 (en) 1988-12-16 1989-12-13 Overexpression of chitinase in transgenic plants

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