CA1305681C - Fluidized bioreactor and cell cultivation process - Google Patents
Fluidized bioreactor and cell cultivation processInfo
- Publication number
- CA1305681C CA1305681C CA000502887A CA502887A CA1305681C CA 1305681 C CA1305681 C CA 1305681C CA 000502887 A CA000502887 A CA 000502887A CA 502887 A CA502887 A CA 502887A CA 1305681 C CA1305681 C CA 1305681C
- Authority
- CA
- Canada
- Prior art keywords
- nutrient medium
- beads
- liquid nutrient
- reaction zone
- liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000004113 cell culture Methods 0.000 title abstract description 18
- 238000006243 chemical reaction Methods 0.000 claims abstract description 82
- 238000011282 treatment Methods 0.000 claims abstract description 17
- 230000004151 fermentation Effects 0.000 claims abstract description 11
- 238000000855 fermentation Methods 0.000 claims abstract description 10
- 239000000203 mixture Substances 0.000 claims abstract description 7
- 238000006213 oxygenation reaction Methods 0.000 claims abstract description 7
- 238000012258 culturing Methods 0.000 claims abstract description 4
- 239000007788 liquid Substances 0.000 claims description 123
- 239000011324 bead Substances 0.000 claims description 110
- 235000015097 nutrients Nutrition 0.000 claims description 79
- 210000004027 cell Anatomy 0.000 claims description 57
- 238000000034 method Methods 0.000 claims description 54
- 102000004190 Enzymes Human genes 0.000 claims description 53
- 108090000790 Enzymes Proteins 0.000 claims description 53
- 239000011942 biocatalyst Substances 0.000 claims description 48
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 32
- 229910052760 oxygen Inorganic materials 0.000 claims description 32
- 239000001301 oxygen Substances 0.000 claims description 32
- 238000009826 distribution Methods 0.000 claims description 24
- 230000003134 recirculating effect Effects 0.000 claims description 23
- 239000000463 material Substances 0.000 claims description 22
- 244000005700 microbiome Species 0.000 claims description 20
- 239000007789 gas Substances 0.000 claims description 17
- 230000005484 gravity Effects 0.000 claims description 13
- 239000012528 membrane Substances 0.000 claims description 12
- 238000005086 pumping Methods 0.000 claims description 10
- 230000000087 stabilizing effect Effects 0.000 claims description 9
- 210000004962 mammalian cell Anatomy 0.000 claims description 8
- 238000010790 dilution Methods 0.000 claims description 7
- 239000012895 dilution Substances 0.000 claims description 7
- 102000008186 Collagen Human genes 0.000 claims description 4
- 108010035532 Collagen Proteins 0.000 claims description 4
- 229920001436 collagen Polymers 0.000 claims description 4
- 229920001282 polysaccharide Polymers 0.000 claims description 4
- 238000011084 recovery Methods 0.000 claims description 4
- 229920001059 synthetic polymer Polymers 0.000 claims description 4
- 150000004676 glycans Chemical class 0.000 claims description 3
- 229910052500 inorganic mineral Inorganic materials 0.000 claims description 3
- 239000002184 metal Substances 0.000 claims description 3
- 229910052751 metal Inorganic materials 0.000 claims description 3
- 239000011707 mineral Substances 0.000 claims description 3
- 239000005017 polysaccharide Substances 0.000 claims description 3
- 150000002739 metals Chemical class 0.000 claims description 2
- 229920005615 natural polymer Polymers 0.000 claims 4
- 239000012530 fluid Substances 0.000 abstract description 25
- 238000005243 fluidization Methods 0.000 abstract description 10
- 239000002609 medium Substances 0.000 description 50
- 239000007787 solid Substances 0.000 description 34
- 239000000047 product Substances 0.000 description 24
- 230000008569 process Effects 0.000 description 21
- 238000013461 design Methods 0.000 description 18
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 12
- 230000000975 bioactive effect Effects 0.000 description 11
- 210000004379 membrane Anatomy 0.000 description 10
- 238000012546 transfer Methods 0.000 description 7
- 239000011800 void material Substances 0.000 description 7
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 6
- 229910002092 carbon dioxide Inorganic materials 0.000 description 6
- 239000001963 growth medium Substances 0.000 description 6
- 239000001569 carbon dioxide Substances 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 230000002503 metabolic effect Effects 0.000 description 4
- 239000002245 particle Substances 0.000 description 4
- 229920000642 polymer Polymers 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 239000000499 gel Substances 0.000 description 3
- 210000004408 hybridoma Anatomy 0.000 description 3
- 230000035699 permeability Effects 0.000 description 3
- 229920001296 polysiloxane Polymers 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 239000000523 sample Substances 0.000 description 3
- 239000000377 silicon dioxide Substances 0.000 description 3
- 238000003860 storage Methods 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- 239000002028 Biomass Substances 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 210000000712 G cell Anatomy 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 238000005276 aerator Methods 0.000 description 2
- 229940057324 biore Drugs 0.000 description 2
- 230000000903 blocking effect Effects 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000006227 byproduct Substances 0.000 description 2
- 229920001525 carrageenan Polymers 0.000 description 2
- 239000000679 carrageenan Substances 0.000 description 2
- 229940113118 carrageenan Drugs 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 239000000919 ceramic Substances 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- 239000000416 hydrocolloid Substances 0.000 description 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 239000002002 slurry Substances 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- HFGHRUCCKVYFKL-UHFFFAOYSA-N 4-ethoxy-2-piperazin-1-yl-7-pyridin-4-yl-5h-pyrimido[5,4-b]indole Chemical compound C1=C2NC=3C(OCC)=NC(N4CCNCC4)=NC=3C2=CC=C1C1=CC=NC=C1 HFGHRUCCKVYFKL-UHFFFAOYSA-N 0.000 description 1
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
- 241000726103 Atta Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 1
- 241000206602 Eukaryota Species 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- LFVLUOAHQIVABZ-UHFFFAOYSA-N Iodofenphos Chemical compound COP(=S)(OC)OC1=CC(Cl)=C(I)C=C1Cl LFVLUOAHQIVABZ-UHFFFAOYSA-N 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 102000003800 Selectins Human genes 0.000 description 1
- 108090000184 Selectins Proteins 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 230000001594 aberrant effect Effects 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 241001148470 aerobic bacillus Species 0.000 description 1
- 238000010564 aerobic fermentation Methods 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- 235000010418 carrageenan Nutrition 0.000 description 1
- 230000005779 cell damage Effects 0.000 description 1
- 230000005465 channeling Effects 0.000 description 1
- 238000001311 chemical methods and process Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000010924 continuous production Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 229910001882 dioxygen Inorganic materials 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 102000034240 fibrous proteins Human genes 0.000 description 1
- 108091005899 fibrous proteins Proteins 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 238000005187 foaming Methods 0.000 description 1
- -1 i.e. Substances 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 229940125396 insulin Drugs 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000012263 liquid product Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000013379 molasses Nutrition 0.000 description 1
- 238000011328 necessary treatment Methods 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 230000001706 oxygenating effect Effects 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 229920000728 polyester Polymers 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- AAEVYOVXGOFMJO-UHFFFAOYSA-N prometryn Chemical compound CSC1=NC(NC(C)C)=NC(NC(C)C)=N1 AAEVYOVXGOFMJO-UHFFFAOYSA-N 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 229960001866 silicon dioxide Drugs 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- 238000009281 ultraviolet germicidal irradiation Methods 0.000 description 1
- 239000002912 waste gas Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
- UHVMMEOXYDMDKI-JKYCWFKZSA-L zinc;1-(5-cyanopyridin-2-yl)-3-[(1s,2s)-2-(6-fluoro-2-hydroxy-3-propanoylphenyl)cyclopropyl]urea;diacetate Chemical compound [Zn+2].CC([O-])=O.CC([O-])=O.CCC(=O)C1=CC=C(F)C([C@H]2[C@H](C2)NC(=O)NC=2N=CC(=CC=2)C#N)=C1O UHVMMEOXYDMDKI-JKYCWFKZSA-L 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/59—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g.hCG [human chorionic gonadotropin]; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/58—Reaction vessels connected in series or in parallel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/16—Particles; Beads; Granular material; Encapsulation
- C12M25/20—Fluidized bed
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2531/00—Microcarriers
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Sustainable Development (AREA)
- Biomedical Technology (AREA)
- Endocrinology (AREA)
- Toxicology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Reproductive Health (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Immunology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US70687285A | 1985-02-28 | 1985-02-28 | |
| US706,872 | 1985-02-28 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1305681C true CA1305681C (en) | 1992-07-28 |
Family
ID=24839418
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000502887A Expired - Fee Related CA1305681C (en) | 1985-02-28 | 1986-02-27 | Fluidized bioreactor and cell cultivation process |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP0215820A1 (de) |
| AU (1) | AU5452986A (de) |
| CA (1) | CA1305681C (de) |
| WO (1) | WO1986005202A1 (de) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140024090A1 (en) * | 2011-12-19 | 2014-01-23 | Shell Oil Company | Method and apparatus for converting a carbon source into a hydrocarbon or hydrocarbon precursor |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1307225C (en) * | 1988-07-19 | 1992-09-08 | David W. Armstrong | Cell culture bioreactor |
| US5102790A (en) * | 1988-12-19 | 1992-04-07 | Baxter International Inc. | Method for culturing animal cells |
| US5187095A (en) * | 1988-12-19 | 1993-02-16 | Baxter International Inc. | Apparatus for culturing animal cells |
| CA2011653A1 (en) * | 1989-03-08 | 1990-09-08 | Hiroshi Deno | Method for culturing plant tissue, apparatus therefor and method for producing metabolite |
| AT394576B (de) * | 1991-01-16 | 1992-05-11 | Vogelbusch Gmbh | Reaktor zur durchfuehrung biologischer reaktionen mittels biokatalysatoren |
| JP3604715B2 (ja) * | 1993-09-07 | 2004-12-22 | サッポロホールディングス株式会社 | 酒類の製造法 |
| DE19528871C2 (de) * | 1994-08-24 | 1997-08-14 | Forschungszentrum Juelich Gmbh | Begasungs-Rohrmodul mit selektiv gasdurchlässiger Schlauchmembranen und damit versehene Reaktoren für Zellkulturtechnik sowie Wirbelschicht-Reaktoren für Zellkultivierung |
| EP0727480A3 (de) * | 1995-02-16 | 2000-04-12 | Forschungszentrum Jülich Gmbh | Verfahren zur Bestimmung von Pharmakokinetik bzw. Toxikokinetik von Testsubstanzen mit Hilfe von in-vitro-Zellkultursystemen und dafür geeignete Vorrichtungen |
| DE59610339D1 (de) * | 1995-02-16 | 2003-05-22 | Forschungszentrum Juelich Gmbh | Verfahren zur Kultivierung von Organfunktionszellen |
| WO1997007228A1 (fr) * | 1995-08-21 | 1997-02-27 | Kyowa Hakko Kogyo Co., Ltd. | Procede de reaction enzymatique immobilisee |
| JP4231563B2 (ja) * | 1997-09-02 | 2009-03-04 | サッポロビール株式会社 | 発酵生産物の製造法 |
| EP2290049B1 (de) * | 1999-09-08 | 2012-08-15 | Levitronix Technologies, LLC | Bioreaktor |
| DE10016877A1 (de) | 2000-04-05 | 2001-10-18 | Scintec Diagnostics Gmbh Zug | (Glyko-)Proteine mit hoher Immunreaktivität sowie ein Verfahren zu ihrer Herstellung |
| DE10127869A1 (de) * | 2001-06-08 | 2003-01-02 | Hoefer Bioreact Gmbh | Bioreaktor mit wenigstens zwei Reaktionskammern |
| DE102007020671A1 (de) * | 2007-05-01 | 2008-11-06 | Justus-Liebig-Universität Giessen | Verfahren und Vorrichtung zum kontaktfreien Materialwachstum in einer strömenden Lösung (FLow suspended solution growth (FSSG)) |
| GB0921836D0 (en) * | 2009-12-14 | 2010-01-27 | Prebble Andrew | Anaerobic reactor |
| EP2576758B1 (de) | 2010-06-07 | 2017-08-02 | Jean-Louis Roux Dit Buisson | Fotobioreaktor mit kontinuierlichem oder halbkontinuierlichem strom und anwendungsverfahren |
| FR3027678B1 (fr) * | 2014-10-24 | 2016-12-30 | Viovy Jean Louis | Procede de detection d'organismes dans un echantillon dilue |
| CN109843417B (zh) | 2016-08-21 | 2022-09-02 | 艾德瓦生物技术股份有限公司 | 生物反应器及其使用方法 |
| CN117844610A (zh) * | 2023-12-31 | 2024-04-09 | 枣庄市杰诺生物酶有限公司 | 一种高纯氨基丁酸的制备装置及其方法 |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2705136A (en) * | 1951-07-09 | 1955-03-29 | Fritz W Glitsch & Sons Inc | Bubble cap riser |
| NL84921C (de) * | 1953-07-07 | |||
| GB1165328A (en) * | 1966-04-15 | 1969-09-24 | Brewing Patents Ltd | Continuous Fermentation Apparatus and Process |
| US4016044A (en) * | 1973-01-10 | 1977-04-05 | Battelle Memorial Institute | Method and apparatus for governing the reaction rate of enzymatic reactions |
| US4048018A (en) * | 1974-08-05 | 1977-09-13 | Coughlin Robert W | Method of carrying out enzyme catalyzed reactions |
| US4032407A (en) * | 1976-02-24 | 1977-06-28 | The United States Of America As Represented By The United States Energy Research & Development Administration | Tapered bed bioreactor |
| US4138290A (en) * | 1976-04-22 | 1979-02-06 | Novo Laboratories, Incorporated | Glucose isomerization under expanded bed conditions |
| GB1584103A (en) * | 1977-06-01 | 1981-02-04 | Ranks Hovis Mcdougall Ltd | Method and apparatus for promoting fermentation |
| NL7709179A (nl) * | 1977-08-19 | 1979-02-21 | Stamicarbon | Werkwijze voor het uitvoeren van enzymatische omzettingen. |
| DE2940446C2 (de) * | 1979-10-05 | 1982-07-08 | B. Braun Melsungen Ag, 3508 Melsungen | Züchtung von tierischen Zellen in Suspensions- und Monolayerkulturen in Fermentationsgefäßen |
| US4397953A (en) * | 1980-02-26 | 1983-08-09 | Process Engineering Company S A | Method and apparatus for anaerobic fermentation |
| GB2073243B (en) * | 1980-03-04 | 1984-12-05 | Tokyo Rikakikai Kk | Continuous fermentor or reactor |
| NZ197992A (en) * | 1980-08-18 | 1984-07-31 | Unisearch Ltd | Anaerobic bacterial degradation of organic materials |
| US4519959A (en) * | 1982-04-14 | 1985-05-28 | Tatsuro Takeuchi | Gas-liquid contacting apparatus |
-
1986
- 1986-02-06 AU AU54529/86A patent/AU5452986A/en not_active Abandoned
- 1986-02-06 EP EP86901270A patent/EP0215820A1/de not_active Withdrawn
- 1986-02-06 WO PCT/US1986/000260 patent/WO1986005202A1/en unknown
- 1986-02-27 CA CA000502887A patent/CA1305681C/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140024090A1 (en) * | 2011-12-19 | 2014-01-23 | Shell Oil Company | Method and apparatus for converting a carbon source into a hydrocarbon or hydrocarbon precursor |
Also Published As
| Publication number | Publication date |
|---|---|
| WO1986005202A1 (en) | 1986-09-12 |
| EP0215820A1 (de) | 1987-04-01 |
| AU5452986A (en) | 1986-09-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA1305681C (en) | Fluidized bioreactor and cell cultivation process | |
| US4978616A (en) | Fluidized cell cultivation process | |
| Feder et al. | The large-scale cultivation of mammalian cells | |
| EP0422149B1 (de) | Statischer oxygenator zur suspensionskultur von tierischen zellen | |
| Zhong | Recent advances in bioreactor engineering | |
| CN110241023B (zh) | 一种用于高密度大规模动物细胞培养的生物反应器及应用 | |
| JP3244701B2 (ja) | バイオマス粒子の生育方法および装置 | |
| Margaritis et al. | Novel bioreactor systems and their applications | |
| US6133019A (en) | Centrifugal fermentation process | |
| US6703217B2 (en) | Methods and devices for remediation and fermentation | |
| JPH0584063A (ja) | 生体触媒により生体的反応を実施する反応装置 | |
| GB1575417A (en) | Processes for promoting interactions in liquid media | |
| US6916652B2 (en) | Biocatalyst chamber encapsulation system for bioremediation and fermentation | |
| Fenge et al. | Cell culture bioreactors | |
| WO1986007605A1 (en) | Fermenter | |
| US6660509B1 (en) | Methods and devices for remediation and fermentation | |
| US20050266548A1 (en) | Biocatalyst chamber encapsulation system for bioremediation and fermentation with improved rotor | |
| US4983517A (en) | Reacting materials | |
| Poulsen et al. | Membrane sparger in bubble column, airlift, and combined membrane–ring sparger bioreactors | |
| WO1999060093A2 (en) | Methods and devices for remediation and fermentation | |
| JPH0343070A (ja) | 固相生体触媒を伴なう生体触媒過程の実施のための装置 | |
| Yamaji et al. | Long-term cultivation of anchorage-independent animal cells immobilized within reticulated biomass support particles in a circulating bed fermentor | |
| WO1993003135A1 (en) | Culture and fermentation method and apparatus | |
| JPH0659210B2 (ja) | 生物反応装置 | |
| JPS63164879A (ja) | 気泡塔型潅流培養方法及びその装置 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MKLA | Lapsed |