CA1297635C - Purification de proteines - Google Patents

Purification de proteines

Info

Publication number
CA1297635C
CA1297635C CA000511855A CA511855A CA1297635C CA 1297635 C CA1297635 C CA 1297635C CA 000511855 A CA000511855 A CA 000511855A CA 511855 A CA511855 A CA 511855A CA 1297635 C CA1297635 C CA 1297635C
Authority
CA
Canada
Prior art keywords
erythropoietin
resin
fluid
recovery
treatment
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA000511855A
Other languages
English (en)
Inventor
Por-Hsiung Lai
Thomas Wayne Strickland
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Amgen K A Inc
Original Assignee
Kirin Amgen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US06/747,119 external-priority patent/US4667016A/en
Application filed by Kirin Amgen Inc filed Critical Kirin Amgen Inc
Priority to CA000616009A priority Critical patent/CA1312994C/fr
Application granted granted Critical
Publication of CA1297635C publication Critical patent/CA1297635C/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/505—Erythropoietin [EPO]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Detergent Compositions (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Cephalosporin Compounds (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
CA000511855A 1985-06-20 1986-06-18 Purification de proteines Expired - Lifetime CA1297635C (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA000616009A CA1312994C (fr) 1985-06-20 1991-02-21 Protein purification

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US06/747,119 US4667016A (en) 1985-06-20 1985-06-20 Erythropoietin purification
US747,119 1985-06-20
US87215286A 1986-06-13 1986-06-13
US872,152 1986-06-13

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA000616009A Division CA1312994C (fr) 1985-06-20 1991-02-21 Protein purification

Publications (1)

Publication Number Publication Date
CA1297635C true CA1297635C (fr) 1992-03-17

Family

ID=27114696

Family Applications (1)

Application Number Title Priority Date Filing Date
CA000511855A Expired - Lifetime CA1297635C (fr) 1985-06-20 1986-06-18 Purification de proteines

Country Status (14)

Country Link
EP (1) EP0228452B1 (fr)
JP (1) JPH0698019B2 (fr)
KR (1) KR930003665B1 (fr)
AT (1) ATE120208T1 (fr)
AU (1) AU606578B2 (fr)
CA (1) CA1297635C (fr)
DE (1) DE3650276T2 (fr)
DK (1) DK175251B1 (fr)
ES (1) ES8801381A1 (fr)
GR (1) GR861624B (fr)
IE (1) IE69343B1 (fr)
IL (1) IL79176A (fr)
PT (1) PT82811B (fr)
WO (1) WO1986007594A1 (fr)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5179199A (en) * 1986-10-20 1993-01-12 Genzyme Corporation Protein purification
IL118201A (en) * 1995-05-11 2004-12-15 Roche Diagnostics Gmbh Preparation comprising a protein with human erythropoietin activity which is free of serum and non-recombinant mammalian protein and process for the preparation thereof
KR100374308B1 (ko) * 1995-07-27 2003-12-18 주식회사 엘지생명과학 재조합차이니스햄스터오바리세포로부터발현된수두바이러스의지피i당단백질의정제방법
ATE489461T1 (de) * 1997-10-16 2010-12-15 Univ Georgia Transgene vögel und proteinproduktion
US6210924B1 (en) 1998-08-11 2001-04-03 Amgen Inc. Overexpressing cyclin D 1 in a eukaryotic cell line
BR9917606A (pt) * 1998-11-06 2002-12-31 Bio Sidus S A Procedimento para a purificação de eritropoetina humana recombinante a partir de sobrenadantes de cultivo de células e eritropoetina humana recombinante obtida com tal procedimento
DK1428878T3 (da) * 2002-12-13 2008-12-08 Bioceuticals Arzneimittel Ag Fremgangsmåde til produktion og oprensning af erythropoietin
US7423139B2 (en) 2004-01-20 2008-09-09 Insight Biopharmaceuticals Ltd. High level expression of recombinant human erythropoietin having a modified 5′-UTR
DE102004027816A1 (de) 2004-06-08 2006-01-05 Bioceuticals Arzneimittel Ag Verfahren zur Reinigung von Erythropoietin
IL165484A0 (en) 2004-11-30 2006-01-15 Applied Research Systems Production of proteins
EP1966232A1 (fr) * 2005-12-23 2008-09-10 Novo Nordisk A/S Purification de proteines par chromatographie preparative en phase inverse (rpc)
KR100900033B1 (ko) * 2007-11-21 2009-06-01 한국생명공학연구원 인간 에리스로포이에틴의 정제방법
DE102008002209A1 (de) * 2008-06-04 2009-12-10 Evonik Degussa Gmbh Verfahren zur Aufreinigung von Erythropoietin
EP2334699B1 (fr) 2008-09-23 2013-09-11 F. Hoffmann-La Roche AG Purification de l'érythropoïétine
DE102008054716A1 (de) 2008-12-16 2010-06-17 Evonik Degussa Gmbh Inprozesskontrolle in einem Verfahren zur Herstellung von EPO
CA2759276A1 (fr) * 2009-04-22 2010-10-28 Bayer Bioscience N.V. Production de structures de n-glycane a antennes multiples dans des plantes
EA022396B1 (ru) 2009-09-23 2015-12-30 Ратиофарм Гмбх Способ очистки рекомбинантного эритропоэтина человека (epo), эритропоэтин, очищенный данным способом, и фармацевтические композиции, содержащие такой эритропоэтин
SG187834A1 (en) 2010-08-20 2013-03-28 Boehringer Ingelheim Int Method for inactivating proteases by ph change in a liquid obtained from a cell culture
WO2013002330A1 (fr) * 2011-06-29 2013-01-03 協和発酵キリン株式会社 Procédé de purification d'une protéine
EP3153522A1 (fr) 2015-10-09 2017-04-12 Hetero Drugs Limited Procédé de purification de l'érythropoïétine et de l'arbépoétine alfa

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IN150740B (fr) * 1978-11-24 1982-12-04 Hoffmann La Roche
JPS5616496A (en) * 1979-07-20 1981-02-17 Sumitomo Chem Co Ltd Recovery of erythropoietin
JPS5653696A (en) * 1979-10-09 1981-05-13 Ajinomoto Co Inc Isolation of erythropoietin by adsorption
JPS57149228A (en) * 1981-03-11 1982-09-14 Ajinomoto Co Inc Novel erythropoietin and its preparation
US4558005A (en) * 1982-09-13 1985-12-10 University Patents, Inc. Monoclonal anti-erythropoietin
JPH0686480B2 (ja) * 1983-02-21 1994-11-02 雪印乳業株式会社 エリスロポエチン製造用モノクローナル抗体
NZ210501A (en) * 1983-12-13 1991-08-27 Kirin Amgen Inc Erythropoietin produced by procaryotic or eucaryotic expression of an exogenous dna sequence
US4568488A (en) * 1984-01-11 1986-02-04 Lee Huang Sylvia Reverse immunoaffinity chromatography purification method
US4677195A (en) * 1985-01-11 1987-06-30 Genetics Institute, Inc. Method for the purification of erythropoietin and erythropoietin compositions

Also Published As

Publication number Publication date
WO1986007594A1 (fr) 1986-12-31
AU606578B2 (en) 1991-02-14
KR930003665B1 (ko) 1993-05-08
DK81387A (da) 1987-02-18
JPH0698019B2 (ja) 1994-12-07
PT82811A (en) 1986-07-01
IL79176A0 (en) 1986-09-30
PT82811B (pt) 1988-04-21
EP0228452A4 (fr) 1989-05-16
KR880000464A (ko) 1988-03-26
AU6123086A (en) 1987-01-13
ES556257A0 (es) 1988-01-01
DK81387D0 (da) 1987-02-18
DE3650276D1 (de) 1995-04-27
ATE120208T1 (de) 1995-04-15
DE3650276T2 (de) 1995-07-27
IE69343B1 (en) 1996-09-04
JPS63503352A (ja) 1988-12-08
EP0228452A1 (fr) 1987-07-15
IE861628L (en) 1986-12-20
GR861624B (en) 1986-10-21
IL79176A (en) 1992-06-21
EP0228452B1 (fr) 1995-03-22
DK175251B1 (da) 2004-07-19
ES8801381A1 (es) 1988-01-01

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