CA1238753A - Method and apparatus for preparing and embedding tissue samples for histological examination - Google Patents
Method and apparatus for preparing and embedding tissue samples for histological examinationInfo
- Publication number
- CA1238753A CA1238753A CA000462768A CA462768A CA1238753A CA 1238753 A CA1238753 A CA 1238753A CA 000462768 A CA000462768 A CA 000462768A CA 462768 A CA462768 A CA 462768A CA 1238753 A CA1238753 A CA 1238753A
- Authority
- CA
- Canada
- Prior art keywords
- tissue
- capsules
- embedding
- mold
- cover
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000010562 histological examination Methods 0.000 title claims abstract description 6
- 238000000034 method Methods 0.000 title abstract description 10
- 239000002775 capsule Substances 0.000 claims abstract description 48
- 239000000463 material Substances 0.000 claims abstract description 37
- 239000011148 porous material Substances 0.000 claims abstract description 25
- 238000007789 sealing Methods 0.000 claims abstract description 9
- 230000000295 complement effect Effects 0.000 claims abstract 2
- 239000002904 solvent Substances 0.000 claims description 16
- 239000012530 fluid Substances 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 238000002156 mixing Methods 0.000 claims description 9
- 238000002360 preparation method Methods 0.000 claims description 4
- 230000006835 compression Effects 0.000 claims description 3
- 238000007906 compression Methods 0.000 claims description 3
- 239000004677 Nylon Substances 0.000 claims description 2
- 229920001778 nylon Polymers 0.000 claims description 2
- 239000011877 solvent mixture Substances 0.000 claims 2
- 239000012188 paraffin wax Substances 0.000 abstract description 29
- 239000007788 liquid Substances 0.000 abstract description 23
- 238000012545 processing Methods 0.000 abstract description 22
- 230000002093 peripheral effect Effects 0.000 abstract description 12
- 238000007667 floating Methods 0.000 abstract description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 60
- 239000000203 mixture Substances 0.000 description 9
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 8
- 238000004821 distillation Methods 0.000 description 8
- 239000008096 xylene Substances 0.000 description 8
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 7
- 230000018044 dehydration Effects 0.000 description 5
- 238000006297 dehydration reaction Methods 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 239000002699 waste material Substances 0.000 description 4
- 230000008859 change Effects 0.000 description 3
- 238000001816 cooling Methods 0.000 description 3
- 238000012864 cross contamination Methods 0.000 description 3
- 238000005520 cutting process Methods 0.000 description 3
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 description 3
- 239000004744 fabric Substances 0.000 description 3
- 239000000834 fixative Substances 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 2
- 108010010803 Gelatin Proteins 0.000 description 2
- 208000034699 Vitreous floaters Diseases 0.000 description 2
- 238000001574 biopsy Methods 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 229920000159 gelatin Polymers 0.000 description 2
- 239000008273 gelatin Substances 0.000 description 2
- 235000019322 gelatine Nutrition 0.000 description 2
- 235000011852 gelatine desserts Nutrition 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 238000000465 moulding Methods 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 229920004943 Delrin® Polymers 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- DHKHKXVYLBGOIT-UHFFFAOYSA-N acetaldehyde Diethyl Acetal Natural products CCOC(C)OCC DHKHKXVYLBGOIT-UHFFFAOYSA-N 0.000 description 1
- 125000002777 acetyl group Chemical class [H]C([H])([H])C(*)=O 0.000 description 1
- 238000004026 adhesive bonding Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- -1 eOg. Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 238000009432 framing Methods 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 238000007373 indentation Methods 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 229920001684 low density polyethylene Polymers 0.000 description 1
- 239000004702 low-density polyethylene Substances 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 229920002635 polyurethane Polymers 0.000 description 1
- 239000004814 polyurethane Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000009877 rendering Methods 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 239000011800 void material Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 238000003466 welding Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
- G01N2001/315—Basket-type carriers for tissues
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US533,192 | 1983-09-16 | ||
| US06/533,192 US4569647A (en) | 1983-09-16 | 1983-09-16 | Apparatus for preparing and embedding tissue samples for histological examination |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1238753A true CA1238753A (en) | 1988-07-05 |
Family
ID=24124889
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000462768A Expired CA1238753A (en) | 1983-09-16 | 1984-09-10 | Method and apparatus for preparing and embedding tissue samples for histological examination |
Country Status (6)
Families Citing this family (59)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4960330A (en) * | 1985-07-16 | 1990-10-02 | Kerschmann Russell L | Image recording apparatus |
| EP0209369A3 (en) * | 1985-07-16 | 1988-07-06 | Russell Lee Kerschmann | Recording visual images |
| JPH0640054B2 (ja) * | 1985-09-20 | 1994-05-25 | 株式会社千代田製作所 | 電子顕微鏡標本作成用包埋装置 |
| US4820504A (en) * | 1986-02-12 | 1989-04-11 | City Of Hope | Multi-specimen tissue blocks and slides |
| JPS631514U (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) * | 1986-06-23 | 1988-01-07 | ||
| US4743556A (en) * | 1987-05-15 | 1988-05-10 | Curtin Matheson Scientific, Inc. | Petri dish |
| GB8915759D0 (en) * | 1989-07-10 | 1989-08-31 | Shandon Scient Ltd | Cell block preparation |
| FI83668C (fi) * | 1989-09-08 | 1991-08-12 | Johan Eric Walter Ahlqvist | Foerfarande och medel foer behandling av cytologiska preparat. |
| JPH0426340U (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) * | 1990-06-28 | 1992-03-02 | ||
| US5080869A (en) * | 1990-08-14 | 1992-01-14 | Mccormick James B | Apparatus and method for preparing tissue samples for histological examination |
| US5612218A (en) * | 1991-12-03 | 1997-03-18 | Busch; Christer | Method of handling biologic matter and means for carrying out the method |
| US5304350A (en) * | 1992-01-16 | 1994-04-19 | Kloehn Instrument Inc. | Slide type analytic device, method, and preparation apparatus |
| US5269671A (en) * | 1992-02-11 | 1993-12-14 | Mccormick James B | Apparatus for embedding tissue samples |
| US5424040A (en) * | 1992-11-09 | 1995-06-13 | Bjornsson; Bjorn L. | Tissue specimen collection kit |
| DE4306233A1 (de) * | 1993-02-27 | 1994-09-08 | Guenter Jahn | Kassette für Gewebeproben |
| DE4306310C2 (de) * | 1993-03-01 | 1996-08-29 | Karel Chaloupek | Gewebeeinbettbehälter mit Einsatz |
| GB2278441B (en) * | 1993-04-06 | 1997-03-05 | Cellpath Plc | Adaptor, holder or container for processing small tissue samples |
| US5427742A (en) * | 1994-04-26 | 1995-06-27 | Holland; Wayne | Tissue processing cassette |
| US5550033A (en) * | 1994-09-26 | 1996-08-27 | Krumdieck; Carlos | Mold plunger and method for embedding tissue samples |
| US5665398A (en) * | 1995-11-20 | 1997-09-09 | Mccormick; James B. | Apparatus for embedding tissue samples |
| US5776298A (en) * | 1996-07-26 | 1998-07-07 | Franks; James W. | Tissue preparation apparatus and method |
| US6017476A (en) * | 1996-09-19 | 2000-01-25 | Renshaw; Anthony A. | Method for embedding and sectioning specimen |
| US20070166834A1 (en) * | 1998-10-05 | 2007-07-19 | Biopath Automation, L.L.C. | Apparatus and method for harvesting and handling tissue samples for biopsy analysis |
| DE10001136C2 (de) | 2000-01-13 | 2003-09-04 | Michael Mengel | Verfahren zur Herstellung von Materialblöcken mit multiplen Untersuchungsproben |
| DE10041228B4 (de) * | 2000-08-22 | 2019-02-14 | Leica Biosystems Nussloch Gmbh | Vorrichtung zur Behandlung von zytologischen oder histologischen Präparaten |
| CA2422232C (en) * | 2000-09-13 | 2011-10-18 | Shandon Inc. | Method and device for preventing chatter in histological samples |
| DE10048724B4 (de) * | 2000-09-29 | 2008-12-04 | Leica Biosystems Nussloch Gmbh | Einrichtung zum strecken von Kryostatschnitten |
| NO315063B1 (no) * | 2001-10-19 | 2003-06-30 | Total Biopsy As | Anordning og fremgangsmåte ved kassett for preparering av biologiske pröver |
| US6767405B2 (en) * | 2002-07-10 | 2004-07-27 | Carmeda Ab | Apparatus and process for coating articles |
| US7179424B2 (en) * | 2002-09-26 | 2007-02-20 | Biopath Automation, L.L.C. | Cassette for handling and holding tissue samples during processing, embedding and microtome procedures, and methods therefor |
| ATE471211T1 (de) * | 2002-09-26 | 2010-07-15 | Biopath Automation Llc | Kassette und einbettanordnung, stufungsvorrichtungen und verfahren zur handhabung von gewebeproben |
| DK1552266T3 (en) * | 2002-09-26 | 2016-02-08 | Biopath Automation Llc | Apparatus and methods for automatic handling and embedding of tissue samples |
| US6913921B2 (en) * | 2002-10-31 | 2005-07-05 | University Of Massachusetts | Rapid cell block embedding method and apparatus |
| US7541161B2 (en) * | 2002-10-31 | 2009-06-02 | University Of Massachusetts | Method and apparatus for preparing cells for microtome sectioning and archiving nucleic acids and proteins |
| US8518348B2 (en) * | 2003-11-26 | 2013-08-27 | Leica Biosystems Richmond, Inc. | Histological specimen retaining device |
| US7521021B2 (en) * | 2003-11-26 | 2009-04-21 | Leica Biosvstems St. Louis Llc | System for in situ processing of a tissue specimen |
| US7238535B2 (en) * | 2004-09-01 | 2007-07-03 | World Properties, Inc. | Test cell for evaluating phosphor |
| US7273587B1 (en) | 2004-12-02 | 2007-09-25 | Collaborative Laboratory Services, Llc | Rapid tissue processing method and apparatus |
| US7273720B1 (en) * | 2004-12-02 | 2007-09-25 | Collaborative Laboratory Services, Llc | Rapid tissue processing method and apparatus |
| US20070116612A1 (en) * | 2005-11-02 | 2007-05-24 | Biopath Automation, L.L.C. | Prefix tissue cassette |
| US7769962B2 (en) * | 2005-12-12 | 2010-08-03 | Jeda Technologies, Inc. | System and method for thread creation and memory management in an object-oriented programming environment |
| US7975586B2 (en) * | 2006-02-06 | 2011-07-12 | Leica Biosystems Richmond, Inc. | Microtome and method of reversibly altering a microtome |
| DK2091440T3 (da) * | 2006-12-12 | 2020-06-15 | Biopath Automation Llc | Biopsibærer med gennemskærbart eftergiveligt cellemateriale |
| JP5230024B2 (ja) * | 2007-02-19 | 2013-07-10 | ディアブ インターナショナル アクチボラゲット | 動的型ツール |
| US7906076B2 (en) * | 2007-07-02 | 2011-03-15 | University Of Massachusetts | Method and apparatus for biopsy sample processing |
| US8118962B2 (en) * | 2007-12-27 | 2012-02-21 | Cytyc Corporation | Method and apparatus for spacing cellular matter in a cell block |
| US20090298172A1 (en) * | 2008-05-28 | 2009-12-03 | Steven Paul Wheeler | Histological specimen treatment apparatus and method |
| CA2748542A1 (en) * | 2008-12-30 | 2010-07-08 | Biopath Automation, L.L.C. | Systems and methods for processing tissue samples for histopathology |
| ES2652615T3 (es) * | 2009-01-22 | 2018-02-05 | Biopath Automation, L.L.C. | Soporte de biopsia seccionable de micrótomo para orientar muestras de tejido |
| DE102009061068B4 (de) * | 2009-04-17 | 2015-05-07 | Leica Biosystems Nussloch Gmbh | Verfahren zur Herstellung mikroskopierbarer Dünnschnitte von Gewebeproben |
| DE102010012546B4 (de) * | 2010-02-25 | 2018-09-13 | Institut für Pathologie Kaufbeuren-Ravensburg GbR (Vertretungsberechtigter Gesellschafter: Herr PD Dr. med. Stephan Schwarz-Furlan, 87600 Kaufbeuren) | Verfahren zur Aufarbeitung und Optimierung von Zell- oder Gewebematerial für die Zytologie bzw. Histologie |
| US9389154B2 (en) * | 2013-03-15 | 2016-07-12 | Leica Biosystems Nussloch Gmbh | Tissue cassette with biasing element |
| US10365189B2 (en) | 2015-05-07 | 2019-07-30 | Steven Wheeler | Histological specimen treatment |
| WO2018209446A1 (en) * | 2017-05-19 | 2018-11-22 | Ambartsoumian Gourgen | Method for identifying embedded samples for microscopy |
| DE102018122173A1 (de) * | 2018-09-11 | 2020-03-12 | Thomas Märsch | Verfahren zum Herstellen eines eine Gewebeprobe enthaltenden Blocks und Gewebeblock |
| CN211235173U (zh) * | 2019-11-26 | 2020-08-11 | 莱卡生物系统努斯洛赫有限责任公司 | 一种组织处理设备 |
| US11635352B2 (en) | 2020-09-08 | 2023-04-25 | Clarapath, Inc. | Enhanced hydration methods and systems for histology |
| FR3117915B1 (fr) * | 2020-12-17 | 2023-04-28 | Commissariat Energie Atomique | Procédé de découpe et de récupération de bâtonnet en cellule blindée |
| WO2022240307A1 (ru) * | 2021-05-13 | 2022-11-17 | Элеонора Ильинична ТЯН | Контейнер для обработки и заливки гистологических образцов |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3128902A (en) * | 1964-04-14 | Histologic tissue receptacle | ||
| US2996762A (en) * | 1958-12-05 | 1961-08-22 | James B Mccormick | Embedding structure and method |
| US3319289A (en) * | 1963-05-20 | 1967-05-16 | Miles Lab | Apparatus for mounting a specimen |
| US3456300A (en) * | 1967-06-05 | 1969-07-22 | John E P Pickett | Composite histologic tissue receptacle and embedding structure |
| US3536040A (en) * | 1967-08-03 | 1970-10-27 | John E P Pickett | Selectively permeable receptacle |
| US3526203A (en) * | 1968-04-08 | 1970-09-01 | Thomas D Kinney | Electron microscopy tissue processor |
| US3527837A (en) * | 1969-08-13 | 1970-09-08 | Petro Tex Chem Corp | Production of isobutylene |
| US3674396A (en) * | 1970-09-21 | 1972-07-04 | Miles Lab | Biological specimen processing and embedding apparatus |
| US3733768A (en) * | 1971-02-12 | 1973-05-22 | B Carls | Storage of histology specimens |
| US3772158A (en) * | 1971-03-29 | 1973-11-13 | Shell Oil Co | Butadiene recovery process |
| FR2176488B1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) * | 1972-03-20 | 1975-03-21 | Inst Francais Du Petrole | |
| FR2190782B1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) * | 1972-06-30 | 1974-12-27 | Rhone Progil | |
| US3982862A (en) * | 1975-04-03 | 1976-09-28 | Pickett John E P | Two-part composite device for histologic tissue processing and embedding |
| US4141312A (en) * | 1977-05-16 | 1979-02-27 | Fisher Scientific Company | Apparatus for histological tissue processing |
| US4224110A (en) * | 1977-12-05 | 1980-09-23 | Mccord James W | Cleaning device |
| US4321330A (en) * | 1980-04-04 | 1982-03-23 | Baker Fraser L | Tissue culture device |
| JPS6335412Y2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) * | 1981-03-05 | 1988-09-20 | ||
| US4352888A (en) * | 1981-12-23 | 1982-10-05 | Custom Plastics, Inc. | Container for holding organic matter during decomposition |
-
1983
- 1983-09-16 US US06/533,192 patent/US4569647A/en not_active Expired - Lifetime
-
1984
- 1984-08-30 EP EP84305951A patent/EP0139424B1/en not_active Expired
- 1984-08-30 AT AT84305951T patent/ATE40210T1/de not_active IP Right Cessation
- 1984-08-30 DE DE8484305951T patent/DE3476265D1/de not_active Expired
- 1984-09-10 CA CA000462768A patent/CA1238753A/en not_active Expired
- 1984-09-17 JP JP59194537A patent/JPS6088347A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6088347A (ja) | 1985-05-18 |
| EP0139424B1 (en) | 1989-01-18 |
| US4569647A (en) | 1986-02-11 |
| EP0139424A3 (en) | 1986-04-23 |
| ATE40210T1 (de) | 1989-02-15 |
| DE3476265D1 (en) | 1989-02-23 |
| JPH0552453B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1993-08-05 |
| EP0139424A2 (en) | 1985-05-02 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MKEX | Expiry |