CA1230300A - Isoenzyme de controle stabilise - Google Patents
Isoenzyme de controle stabiliseInfo
- Publication number
- CA1230300A CA1230300A CA000453361A CA453361A CA1230300A CA 1230300 A CA1230300 A CA 1230300A CA 000453361 A CA000453361 A CA 000453361A CA 453361 A CA453361 A CA 453361A CA 1230300 A CA1230300 A CA 1230300A
- Authority
- CA
- Canada
- Prior art keywords
- reagent
- stabilizing means
- lactose
- isoenzyme
- plexiform
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 108010044467 Isoenzymes Proteins 0.000 title claims abstract description 77
- 230000000087 stabilizing effect Effects 0.000 claims abstract description 73
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 70
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims abstract description 30
- 239000008101 lactose Substances 0.000 claims abstract description 30
- 235000000346 sugar Nutrition 0.000 claims abstract description 22
- 238000000034 method Methods 0.000 claims abstract description 11
- 239000002738 chelating agent Substances 0.000 claims abstract description 5
- 150000008163 sugars Chemical class 0.000 claims abstract description 3
- 102000004190 Enzymes Human genes 0.000 claims description 27
- 108090000790 Enzymes Proteins 0.000 claims description 27
- 230000000694 effects Effects 0.000 claims description 26
- 230000008707 rearrangement Effects 0.000 claims description 21
- -1 monosaccharide sugars Chemical class 0.000 claims description 19
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 claims description 17
- GUBGYTABKSRVRQ-CUHNMECISA-N D-Cellobiose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-CUHNMECISA-N 0.000 claims description 17
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 claims description 16
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 claims description 16
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 claims description 16
- 229930195725 Mannitol Natural products 0.000 claims description 16
- 239000000594 mannitol Substances 0.000 claims description 16
- 235000010355 mannitol Nutrition 0.000 claims description 16
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 claims description 15
- 239000007864 aqueous solution Substances 0.000 claims description 10
- 108090000623 proteins and genes Proteins 0.000 claims description 10
- 102000004169 proteins and genes Human genes 0.000 claims description 9
- 239000000243 solution Substances 0.000 claims description 8
- 150000001455 metallic ions Chemical class 0.000 claims description 6
- 229910019142 PO4 Inorganic materials 0.000 claims description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 4
- 239000010452 phosphate Substances 0.000 claims description 4
- 230000000845 anti-microbial effect Effects 0.000 claims 1
- 230000002401 inhibitory effect Effects 0.000 claims 1
- 230000000813 microbial effect Effects 0.000 claims 1
- 150000002016 disaccharides Chemical class 0.000 abstract description 2
- 150000002772 monosaccharides Chemical class 0.000 abstract description 2
- 102000003855 L-lactate dehydrogenase Human genes 0.000 description 12
- 108700023483 L-lactate dehydrogenases Proteins 0.000 description 12
- 210000001519 tissue Anatomy 0.000 description 12
- 239000000047 product Substances 0.000 description 9
- 238000007710 freezing Methods 0.000 description 7
- 230000008014 freezing Effects 0.000 description 7
- 235000018102 proteins Nutrition 0.000 description 5
- 239000000470 constituent Substances 0.000 description 4
- DDRJAANPRJIHGJ-UHFFFAOYSA-N creatinine Chemical compound CN1CC(=O)NC1=N DDRJAANPRJIHGJ-UHFFFAOYSA-N 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 102100036475 Alanine aminotransferase 1 Human genes 0.000 description 2
- 108010082126 Alanine transaminase Proteins 0.000 description 2
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 2
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 2
- 108700016171 Aspartate ammonia-lyases Proteins 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical class O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 108090000340 Transaminases Proteins 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 229940109239 creatinine Drugs 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 238000004108 freeze drying Methods 0.000 description 2
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 210000003205 muscle Anatomy 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 102000014898 transaminase activity proteins Human genes 0.000 description 2
- 229930024421 Adenine Natural products 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- 229910002012 Aerosil® Inorganic materials 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical group OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 101710107035 Gamma-glutamyltranspeptidase Proteins 0.000 description 1
- 108010024636 Glutathione Proteins 0.000 description 1
- 101710173228 Glutathione hydrolase proenzyme Proteins 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 108091000080 Phosphotransferase Proteins 0.000 description 1
- 102000001253 Protein Kinase Human genes 0.000 description 1
- LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 description 1
- 229960000643 adenine Drugs 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 230000000747 cardiac effect Effects 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 230000005496 eutectics Effects 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 102000006640 gamma-Glutamyltransferase Human genes 0.000 description 1
- 230000008571 general function Effects 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 229960003180 glutathione Drugs 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000002107 myocardial effect Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 102000020233 phosphotransferase Human genes 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 108060006633 protein kinase Proteins 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 125000003396 thiol group Chemical group [H]S* 0.000 description 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Fats And Perfumes (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US50121383A | 1983-06-06 | 1983-06-06 | |
| US501,213 | 1983-06-06 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1230300A true CA1230300A (fr) | 1987-12-15 |
Family
ID=23992572
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000453361A Expired CA1230300A (fr) | 1983-06-06 | 1984-05-02 | Isoenzyme de controle stabilise |
Country Status (6)
| Country | Link |
|---|---|
| JP (1) | JP2596911B2 (fr) |
| AU (1) | AU583474B2 (fr) |
| CA (1) | CA1230300A (fr) |
| DK (1) | DK275184A (fr) |
| IL (1) | IL72030A0 (fr) |
| NO (1) | NO842258L (fr) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1226795A (fr) * | 1983-06-06 | 1987-09-15 | Michael K. Hoskins | Produits de controle stabilises de la coagulation |
| US4663295A (en) * | 1983-06-29 | 1987-05-05 | Ciba Corning Diagnostics Corp. | Estrogen-progesterone control reagents and methods for making same |
| CA1226794A (fr) * | 1983-06-06 | 1987-09-15 | Michael K. Hoskins | Produit de controle stabilise multiparametre |
| JP2575648B2 (ja) * | 1986-04-24 | 1997-01-29 | 国際試薬株式会社 | クレアチンキナ−ゼの安定化方法 |
| IT1394539B1 (it) * | 2009-05-19 | 2012-07-05 | Sentinel Ch S P A | Uso di uno stabilizzante delle polimerasi per la liofilizzazione e la preparazione di kit pronti per l'uso |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS4834912A (fr) * | 1971-09-09 | 1973-05-23 | ||
| JPS589688A (ja) * | 1981-07-06 | 1983-01-20 | Toyobo Co Ltd | 安定な酵素組成物 |
| JPS5865218A (ja) * | 1981-10-13 | 1983-04-18 | Kowa Co | 組織プラスミノーゲンアクチベーターの製造方法 |
| JPS58107178A (ja) * | 1981-12-22 | 1983-06-25 | Mitsui Toatsu Chem Inc | ベ−タ−ガラクトシダ−ゼ又はその複合体の安定な凍結乾燥品 |
| JPS58134991A (ja) * | 1981-12-28 | 1983-08-11 | Takeda Chem Ind Ltd | セラチオペプチタ−ゼの安定化法 |
| CA1226795A (fr) * | 1983-06-06 | 1987-09-15 | Michael K. Hoskins | Produits de controle stabilises de la coagulation |
| US4663295A (en) * | 1983-06-29 | 1987-05-05 | Ciba Corning Diagnostics Corp. | Estrogen-progesterone control reagents and methods for making same |
| CA1226794A (fr) * | 1983-06-06 | 1987-09-15 | Michael K. Hoskins | Produit de controle stabilise multiparametre |
-
1984
- 1984-05-02 CA CA000453361A patent/CA1230300A/fr not_active Expired
- 1984-06-04 DK DK275184A patent/DK275184A/da not_active Application Discontinuation
- 1984-06-05 IL IL72030A patent/IL72030A0/xx unknown
- 1984-06-05 AU AU29085/84A patent/AU583474B2/en not_active Ceased
- 1984-06-05 NO NO842258A patent/NO842258L/no unknown
- 1984-06-06 JP JP59114690A patent/JP2596911B2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| AU583474B2 (en) | 1989-05-04 |
| DK275184A (da) | 1984-12-07 |
| NO842258L (no) | 1984-12-07 |
| DK275184D0 (da) | 1984-06-04 |
| IL72030A0 (en) | 1984-10-31 |
| JPS606192A (ja) | 1985-01-12 |
| JP2596911B2 (ja) | 1997-04-02 |
| AU2908584A (en) | 1984-12-13 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MKEX | Expiry |