CA1155041A - Fluorescent nucleic acid stains - Google Patents

Fluorescent nucleic acid stains

Info

Publication number
CA1155041A
CA1155041A CA000371297A CA371297A CA1155041A CA 1155041 A CA1155041 A CA 1155041A CA 000371297 A CA000371297 A CA 000371297A CA 371297 A CA371297 A CA 371297A CA 1155041 A CA1155041 A CA 1155041A
Authority
CA
Canada
Prior art keywords
nucleus
iodide
fluorescent dye
lower alkyl
dimethylaminopropyl
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
CA000371297A
Other languages
English (en)
French (fr)
Inventor
Michael E. Jolley
Chao-Huei J. Wang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Abbott Laboratories
Original Assignee
Abbott Laboratories
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Laboratories filed Critical Abbott Laboratories
Application granted granted Critical
Publication of CA1155041A publication Critical patent/CA1155041A/en
Expired legal-status Critical Current

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5094Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Ecology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA000371297A 1980-04-21 1981-02-19 Fluorescent nucleic acid stains Expired CA1155041A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14232180A 1980-04-21 1980-04-21
US142,321 1980-04-21

Publications (1)

Publication Number Publication Date
CA1155041A true CA1155041A (en) 1983-10-11

Family

ID=22499396

Family Applications (1)

Application Number Title Priority Date Filing Date
CA000371297A Expired CA1155041A (en) 1980-04-21 1981-02-19 Fluorescent nucleic acid stains

Country Status (6)

Country Link
JP (1) JPS56158944A (ru)
BE (1) BE888509A (ru)
CA (1) CA1155041A (ru)
DE (1) DE3115278C2 (ru)
FR (1) FR2480943A1 (ru)
GB (1) GB2074340B (ru)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4500509A (en) * 1981-03-11 1985-02-19 Lawrence Kass Metachromatic dye sorption and fluorescent light emmisive means for differential determination of developmental stages of neutrophilic granulocytic cells and other leukocytes
US4555396A (en) * 1982-12-22 1985-11-26 Eastman Kodak Company Use of pyrylium and thiapyrylium compounds as biological stains
US4840784A (en) * 1982-12-22 1989-06-20 Eastman Kodak Company Use of pyrylium and thiapyrylium compounds as biological stains
NZ207393A (en) * 1983-08-05 1987-03-31 Neal Lloyd First Staining dna in living cells
JPS61280565A (ja) * 1985-06-06 1986-12-11 Toa Medical Electronics Co Ltd フロ−サイトメトリ−用網状血球測定試薬
JPH076979B2 (ja) * 1986-09-10 1995-01-30 東亜医用電子株式会社 フロ−サイトメトリ−による白血球分類に使用される試薬
DE69424079T2 (de) * 1993-02-22 2000-09-07 Sysmex Corp., Kobe Ein Reagenz zum Nachweis von malariainfizierten Zellen und ein Nachweis-Verfahren für malariainfizierte Zellen unter Verwendung desselben
US5436134A (en) * 1993-04-13 1995-07-25 Molecular Probes, Inc. Cyclic-substituted unsymmetrical cyanine dyes
US5534416A (en) * 1993-04-13 1996-07-09 Molecular Probes, Inc. Fluorescent viability assay using cyclic-substituted unsymmetrical cyanine dyes
US5445946A (en) * 1993-04-13 1995-08-29 Molecular Probes, Inc. Intravacuolar stains for yeast and other fungi
US5545535A (en) * 1993-04-13 1996-08-13 Molecular Probes, Inc. Fluorescent assay for bacterial gram reaction
US5571727A (en) * 1993-10-07 1996-11-05 Matsushita Electric Industrial Co., Ltd. Labelling colors for detecting cocaine or methamphetamine, method of preparing the same and detector for cocaine or methamphetamine
CN1036612C (zh) * 1994-03-08 1997-12-03 中国人民解放军第304医院 透射电子显微镜生物样品超薄切片染色液
ATE280243T1 (de) * 1994-04-29 2004-11-15 Johnson & Johnson Clin Diag Homogenes verfahren zum nachweis von doppelstrang-nukleinsäuren mittels fluoreszierender farbstoffe und dafür nützliche kits
EP0708334B1 (en) * 1994-10-20 2001-05-23 Sysmex Corporation Reagent and method for analyzing solid components in urine
US7776529B2 (en) 2003-12-05 2010-08-17 Life Technologies Corporation Methine-substituted cyanine dye compounds
EP1885718B1 (en) 2005-05-11 2017-03-15 Life Technologies Corporation Fluorescent chemical compounds having high selectivity for double stranded dna, and methods for their use
US7781187B2 (en) * 2005-12-30 2010-08-24 Corning Incorporated Fluorescent dyes
JP2013040136A (ja) * 2011-08-17 2013-02-28 Tosoh Corp 4−アルコキシ−2−(4−アミノスチリル)ベンゾチアゾリウム塩、その製造方法およびそれを用いる核酸の検出方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1473945A (en) * 1973-05-24 1977-05-18 Gen Electric Method for preparing slides for blood evaluation
DE2515966C3 (de) * 1975-04-11 1979-11-22 Boehringer Mannheim Gmbh, 6800 Mannheim Vorgefärbte Objektträger für die Blutunte rsuchung

Also Published As

Publication number Publication date
GB2074340A (en) 1981-10-28
BE888509A (fr) 1981-10-21
DE3115278C2 (de) 1983-08-18
FR2480943B1 (ru) 1984-03-16
DE3115278A1 (de) 1982-03-18
GB2074340B (en) 1984-02-15
JPS56158944A (en) 1981-12-08
FR2480943A1 (fr) 1981-10-23
JPS6361622B2 (ru) 1988-11-29

Similar Documents

Publication Publication Date Title
CA1155041A (en) Fluorescent nucleic acid stains
US4544546A (en) Fluorescent nucleic acid stains
JP4831914B2 (ja) 未成熟赤血球細胞における核酸の検出用色素及び方法
Cesarone et al. Improved microfluorometric DNA determination in biological material using 33258 Hoechst
US4581223A (en) Individual leukocyte determination by means of differential metachromatic dye sorption
Novo et al. Accurate flow cytometric membrane potential measurement in bacteria using diethyloxacarbocyanine and a ratiometric technique
Determann et al. Ultraviolet fluorescence excitation and emission spectroscopy of marine algae and bacteria
US5314805A (en) Dual-fluorescence cell viability assay using ethidium homodimer and calcein AM
DE69330818T2 (de) Fluoreszenzfarbstoff, Pyryliumsalze oder ähnhliche Salze enthaltend, Nachweisverfahren von Nukleinsäure durch Verwendung desselben
ES2357524T3 (es) Procedimiento para la identificación y el recuento de células biológicas.
Otto High-resolution analysis of nuclear DNA employing the fluorochrome DAPI
CA1205365A (en) Metachromatic dye sorption and fluorescent light emissive means for determination of developmental stages of neutrophilic granulocytic cells and other leukocytes
CA2024166C (en) Compounds and reagent compositions and their use in the quantitative determination of reticulocytes in whole blood
US5437980A (en) Phenanthridium dye staining of nucleic acids in living cells
FI72611B (fi) Blandning som laempar sig foer testning av biologiska vaevnader och/eller vaetskor och foerfarande foer anvaendning av denna blandning.
CN102947266A (zh) 分析细胞或含有核酸的其它生物材料的方法
GB2095402A (en) Metachromatic dye sorption means for differential determination of developmental stages of neutrophilic granulocytic cells and other leukocytes
Cornelisse et al. A new type of two-color fluorescence staining for cytology specimens.
CA2273959C (en) Helium-neon excitable reticulocyte dyes derivable from halolepidines
US5470751A (en) Reagent for detecting malaria infected cells and a detecting method for malaria infected cells using the same
Ghali et al. Fluorescence of Pneumocystis carinii in Papanicolaou smears
Hull et al. Cytofluorometric determination of nuclear DNA in living and preserved algae
Cole Induced fluorescence in gametophytes of some Laminariales
Ortolani Fluorochromes That Bind Nucleic Acids
Shapiro Fluorescent probes

Legal Events

Date Code Title Description
MKEX Expiry