CA1055489A - Method of separating heme iron compounds from globin - Google Patents
Method of separating heme iron compounds from globinInfo
- Publication number
- CA1055489A CA1055489A CA229,318A CA229318A CA1055489A CA 1055489 A CA1055489 A CA 1055489A CA 229318 A CA229318 A CA 229318A CA 1055489 A CA1055489 A CA 1055489A
- Authority
- CA
- Canada
- Prior art keywords
- liquid
- globin
- iron component
- ethanol
- heme iron
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 102000018146 globin Human genes 0.000 title claims abstract description 21
- 108060003196 globin Proteins 0.000 title claims abstract description 21
- 238000000034 method Methods 0.000 title claims abstract description 18
- -1 heme iron compounds Chemical class 0.000 title 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 42
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims abstract description 42
- 239000007788 liquid Substances 0.000 claims abstract description 35
- 229910052742 iron Inorganic materials 0.000 claims abstract description 21
- 239000000126 substance Substances 0.000 claims abstract description 15
- 239000000203 mixture Substances 0.000 claims abstract description 11
- 239000003960 organic solvent Substances 0.000 claims abstract description 11
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims abstract description 6
- 239000008280 blood Substances 0.000 claims abstract description 4
- 210000004369 blood Anatomy 0.000 claims abstract description 4
- 150000003839 salts Chemical class 0.000 claims abstract description 4
- 239000001103 potassium chloride Substances 0.000 claims abstract description 3
- 235000011164 potassium chloride Nutrition 0.000 claims abstract description 3
- 239000001509 sodium citrate Substances 0.000 claims abstract description 3
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims abstract description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 12
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 12
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical group [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 12
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 9
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 9
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 9
- 235000002639 sodium chloride Nutrition 0.000 claims description 9
- 239000006228 supernatant Substances 0.000 claims description 9
- 150000003278 haem Chemical class 0.000 claims description 7
- 239000011780 sodium chloride Substances 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 5
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 claims description 3
- 238000005054 agglomeration Methods 0.000 claims description 3
- 230000002776 aggregation Effects 0.000 claims description 3
- 238000005119 centrifugation Methods 0.000 claims description 3
- 230000001376 precipitating effect Effects 0.000 claims description 3
- 102000004169 proteins and genes Human genes 0.000 claims description 3
- 108090000623 proteins and genes Proteins 0.000 claims description 3
- 238000000926 separation method Methods 0.000 claims description 3
- 238000001816 cooling Methods 0.000 claims description 2
- 230000000694 effects Effects 0.000 claims description 2
- 239000010695 polyglycol Substances 0.000 claims description 2
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 claims description 2
- 239000004299 sodium benzoate Substances 0.000 claims description 2
- 235000010234 sodium benzoate Nutrition 0.000 claims description 2
- 229910052938 sodium sulfate Inorganic materials 0.000 claims description 2
- 235000011152 sodium sulphate Nutrition 0.000 claims description 2
- 229960005150 glycerol Drugs 0.000 claims 2
- 235000011187 glycerol Nutrition 0.000 claims 2
- 239000001166 ammonium sulphate Substances 0.000 claims 1
- KSFOVUSSGSKXFI-GAQDCDSVSA-N CC1=C/2NC(\C=C3/N=C(/C=C4\N\C(=C/C5=N/C(=C\2)/C(C=C)=C5C)C(C=C)=C4C)C(C)=C3CCC(O)=O)=C1CCC(O)=O Chemical compound CC1=C/2NC(\C=C3/N=C(/C=C4\N\C(=C/C5=N/C(=C\2)/C(C=C)=C5C)C(C=C)=C4C)C(C)=C3CCC(O)=O)=C1CCC(O)=O KSFOVUSSGSKXFI-GAQDCDSVSA-N 0.000 abstract description 2
- 238000000354 decomposition reaction Methods 0.000 abstract description 2
- 229950003776 protoporphyrin Drugs 0.000 abstract description 2
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 abstract 2
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 19
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 9
- 102000001554 Hemoglobins Human genes 0.000 description 8
- 108010054147 Hemoglobins Proteins 0.000 description 8
- 239000002244 precipitate Substances 0.000 description 7
- 239000002904 solvent Substances 0.000 description 4
- ZWEHNKRNPOVVGH-UHFFFAOYSA-N 2-Butanone Chemical compound CCC(C)=O ZWEHNKRNPOVVGH-UHFFFAOYSA-N 0.000 description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 239000002253 acid Substances 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 2
- 229960004319 trichloroacetic acid Drugs 0.000 description 2
- 241000237074 Centris Species 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 229920000151 polyglycol Polymers 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D487/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00
- C07D487/22—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00 in which the condensed system contains four or more hetero rings
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/06—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from blood
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/795—Porphyrin- or corrin-ring-containing peptides
- C07K14/805—Haemoglobins; Myoglobins
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Health & Medical Sciences (AREA)
- Food Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Gastroenterology & Hepatology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Polymers & Plastics (AREA)
- Zoology (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Extraction Or Liquid Replacement (AREA)
- Fodder In General (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE7407882-5A SE389596B (sv) | 1974-06-14 | 1974-06-14 | Saett att ur en vaetska innehallande blodsubstanser separera jaernfoereningar fran globin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1055489A true CA1055489A (en) | 1979-05-29 |
Family
ID=20321445
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA229,318A Expired CA1055489A (en) | 1974-06-14 | 1975-06-13 | Method of separating heme iron compounds from globin |
Country Status (18)
| Country | Link |
|---|---|
| AR (1) | AR209770A1 (OSRAM) |
| AT (1) | AT346672B (OSRAM) |
| AU (1) | AU502954B2 (OSRAM) |
| BR (1) | BR7503722A (OSRAM) |
| CA (1) | CA1055489A (OSRAM) |
| DE (1) | DE2526596C2 (OSRAM) |
| DK (1) | DK150173B (OSRAM) |
| ES (1) | ES438483A1 (OSRAM) |
| FI (1) | FI59195C (OSRAM) |
| FR (1) | FR2274230A1 (OSRAM) |
| HU (1) | HU174189B (OSRAM) |
| IE (1) | IE49166B1 (OSRAM) |
| PL (1) | PL94776B1 (OSRAM) |
| RO (1) | RO63791A (OSRAM) |
| SE (1) | SE389596B (OSRAM) |
| SU (1) | SU910135A3 (OSRAM) |
| YU (1) | YU147475A (OSRAM) |
| ZA (1) | ZA753806B (OSRAM) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4761472A (en) * | 1986-03-12 | 1988-08-02 | Basf Aktiengesellschaft | Isolation and purification of hemin |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1126653A (en) * | 1978-12-22 | 1982-06-29 | Jan H. Luijerink | Process of preparing blood cell protein and heme from hemoglobin |
| SE440596B (sv) * | 1980-04-03 | 1985-08-12 | Paul Goran Sigvard Lindroos | Forfarande for framstellning av ett heme-koncentrat ur en blandning av heme och blodsubstans erhallen vid spaltning av hemoglobin |
| FR2535173A1 (fr) * | 1982-11-03 | 1984-05-04 | Protein Sa | Produits obtenus a partir de sang d'animaux d'abattoirs et leur procede d'obtention |
| JPS59128337A (ja) * | 1983-01-11 | 1984-07-24 | Riyoushiyoku Kenkyukai | 血液グロビン及びヘムの回収方法 |
| FR2548671B1 (fr) * | 1983-07-07 | 1986-05-02 | Merieux Inst | Procede de preparation de globine a partir d'hemoglobine et globine obtenue par ce procede |
| DE68918083T2 (de) * | 1988-11-18 | 1995-01-05 | Tosoh Corp | Verfahren zur reinigung von blutplasma. |
| ATE220678T1 (de) * | 1996-12-20 | 2002-08-15 | Fraunhofer Ges Forschung | Verfahren zur gewinnung von hämin aus schlachtblut |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE178902C (OSRAM) * | 1905-10-16 | |||
| SE348942B (OSRAM) * | 1970-06-02 | 1972-09-18 | Statens Bakteriologiska Labor |
-
1974
- 1974-06-14 SE SE7407882-5A patent/SE389596B/xx not_active IP Right Cessation
-
1975
- 1975-05-29 AU AU81668/75A patent/AU502954B2/en not_active Expired
- 1975-06-04 FI FI751643A patent/FI59195C/fi not_active IP Right Cessation
- 1975-06-04 AR AR259065A patent/AR209770A1/es active
- 1975-06-06 YU YU01474/75A patent/YU147475A/xx unknown
- 1975-06-12 BR BR4779/75D patent/BR7503722A/pt unknown
- 1975-06-12 ES ES438483A patent/ES438483A1/es not_active Expired
- 1975-06-12 HU HU75LI279A patent/HU174189B/hu unknown
- 1975-06-12 PL PL1975181157A patent/PL94776B1/pl unknown
- 1975-06-12 FR FR7518352A patent/FR2274230A1/fr active Granted
- 1975-06-13 DE DE2526596A patent/DE2526596C2/de not_active Expired
- 1975-06-13 ZA ZA3806A patent/ZA753806B/xx unknown
- 1975-06-13 DK DK266675AA patent/DK150173B/da not_active Application Discontinuation
- 1975-06-13 SU SU752143704A patent/SU910135A3/ru active
- 1975-06-13 AT AT456575A patent/AT346672B/de not_active IP Right Cessation
- 1975-06-13 IE IE1335/75A patent/IE49166B1/en unknown
- 1975-06-13 CA CA229,318A patent/CA1055489A/en not_active Expired
- 1975-06-14 RO RO7500082550A patent/RO63791A/ro unknown
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4761472A (en) * | 1986-03-12 | 1988-08-02 | Basf Aktiengesellschaft | Isolation and purification of hemin |
Also Published As
| Publication number | Publication date |
|---|---|
| AR209770A1 (es) | 1977-05-31 |
| AU8166875A (en) | 1976-12-02 |
| PL94776B1 (pl) | 1977-08-31 |
| IE49166B1 (en) | 1985-08-21 |
| ATA456575A (de) | 1978-03-15 |
| AT346672B (de) | 1978-11-27 |
| FI59195C (fi) | 1981-07-10 |
| FR2274230A1 (fr) | 1976-01-09 |
| YU147475A (en) | 1982-05-31 |
| DK266675A (da) | 1975-12-15 |
| DE2526596C2 (de) | 1984-11-29 |
| HU174189B (hu) | 1979-11-28 |
| AU502954B2 (en) | 1979-08-16 |
| DE2526596A1 (de) | 1976-01-02 |
| RO63791A (fr) | 1978-12-15 |
| FI751643A7 (OSRAM) | 1975-12-15 |
| FI59195B (fi) | 1981-03-31 |
| ZA753806B (en) | 1977-01-26 |
| SE389596B (sv) | 1976-11-15 |
| SU910135A3 (ru) | 1982-02-28 |
| ES438483A1 (es) | 1977-02-01 |
| DK150173B (da) | 1986-12-29 |
| BR7503722A (pt) | 1976-06-29 |
| SE7407882L (sv) | 1975-12-15 |
| FR2274230B1 (OSRAM) | 1981-03-27 |
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