CA1055489A - Method of separating heme iron compounds from globin - Google Patents
Method of separating heme iron compounds from globinInfo
- Publication number
- CA1055489A CA1055489A CA229,318A CA229318A CA1055489A CA 1055489 A CA1055489 A CA 1055489A CA 229318 A CA229318 A CA 229318A CA 1055489 A CA1055489 A CA 1055489A
- Authority
- CA
- Canada
- Prior art keywords
- liquid
- globin
- iron component
- ethanol
- heme iron
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D487/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00
- C07D487/22—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00 in which the condensed system contains four or more hetero rings
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/06—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from blood
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/795—Porphyrin- or corrin-ring-containing peptides
- C07K14/805—Haemoglobins; Myoglobins
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Food Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Gastroenterology & Hepatology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Polymers & Plastics (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Extraction Or Liquid Replacement (AREA)
- Fodder In General (AREA)
Abstract
ABSTRACT
There is provided an improved method, suitable for commercial use, for separating from a liquid containing blood substances the iron protopor-phyrin and dirivatives or decomposition products thereof, the so-called iron component, from globin, characterized in, that the liquid is cooled, preferably to a temperature of from 0° to 20°C, that the pH of the liquid is adjusted to a value lower then 4.5, preferably to a value of from 2,5 to 4.5, that an organic solvent with dehydrating properties is added to said liquid, for ex-ample ethanol or mixtures of ethanol with glycol, in an amount liquid, preferably at least 75 per cent of volume, and thereby maintaining the above indicated temperature, that the precipitated iron component is separated from said liquid, preferably by addition of salts, such as potassium chloride or sodium citrate, whereafter the globin is precipitated from the liquid.
There is provided an improved method, suitable for commercial use, for separating from a liquid containing blood substances the iron protopor-phyrin and dirivatives or decomposition products thereof, the so-called iron component, from globin, characterized in, that the liquid is cooled, preferably to a temperature of from 0° to 20°C, that the pH of the liquid is adjusted to a value lower then 4.5, preferably to a value of from 2,5 to 4.5, that an organic solvent with dehydrating properties is added to said liquid, for ex-ample ethanol or mixtures of ethanol with glycol, in an amount liquid, preferably at least 75 per cent of volume, and thereby maintaining the above indicated temperature, that the precipitated iron component is separated from said liquid, preferably by addition of salts, such as potassium chloride or sodium citrate, whereafter the globin is precipitated from the liquid.
Description
1~55489 The present invention relates to a way of treating liquids contain-ing blood substanc~s, particularly a way of separating from such liquids ~he globin contained in the liquid from ~he iron protoporphyrin or derivatives and/or cleavage products of it.
There are already known ways of separating, on a laboratory scale, the iron component and the globin chromatographically or by ex~raction, in which solvents like methylethyl ketone, acetone and dimethyl formamide (phor-mamide~ are used.
The chromatographic procedure is less suitable on a technical scale;
and the extraction with solvents yields a product which is unsuitable for use in feeds or foodstuffs.
In the present invention, the employment of a simple process tech-nique is made possible by uslng an organic solvent with dehydrating properties and preferably by adding a medium with an ion-strength-increasing and/or de-hydrating effect, for example, sodium chloride, sodium sulfate or ammonium sulfate, sodium benzoate or polyglycol with a possible addition of pH-regul-ating media like nonorganic acids, e.g. hydrochloric acid and sulphuric acid9 ~-or organic acids, e.g. acetic acid or citric acid. Consequently ~he invention constitutes a technically and economically practicable method for separating the iron component from the globin permitting the use of the globin in feeds and foodstuffs.
Thus, the invention provides a method for separating the heme iron component from the globin in a liquid containing blood substances, comprising cooling the liquid, adding to the liquid an organic solvent selected from the group consisting of ethanol, methanol, mixtures of ethanol and glycerol and mixtures of methanol ~nd glycerol in an amount of ~0 to 90 per cent by volume based on the total amount of khe liquid, adjusting the p~l to a value belo~
4.S, separating the heme iron component agglomerate, and precipitating the globin from the liquid. The liquid is preferably cooled to a temperature of from 0C to -20JC and ~he pH of the liquid is preferably adjusted ~o a value of from 2.5 to ~.5. ~e amount of organic solvent is preferably a~ least 75 per cent by volume, based on the total amount Oe liquid, while the iron component o~s precipitated and separated from said liquid preferably by addi-1055~39 tion of salts, such as potassium chloride or sodium citrate, whereafter theglobin is precipitated from the liquid.
According to a special form of execution of the way in accordance with the invention part of the iron component is first separated, e.g. exist-ing as an aggregate in concentrated solutions or adsorbed on protein material, preferably by centrifugation. The agglomeration can be effected by adding for instance sodium chloride. From the supernatant thus obtained the globin in then precipitated by adding ion-strength-increasing and/or dehydrating media while the iron component is retained in the liquid phase in a fluid and/or finely dispersed form.
Thus in a further embodi~ent, the method provided above is character-ized in that the organic solvent is added in two stages, a small amount of the solvent being added in a first stage preferably by adding sodium chloride or another substance that favours agglomeration and/or absorption of the iron component particles on the protein material, preferably denaturated, after which the agglomerate obtained is separated, e.g. by centrifugation, and then further organic solvent is added in a second stage for separation of the globin ~ ~ -from the remaining part of the iron component, which then is in a solved and/
or finely dispersed form.
The environment created by the high content of solvent, e.g. ethanol, and the low content of water with a certain ion strength and a low pH-value is evidently suitable for retaining the finely dispersed and the fluid form respectively of the iron component and the possible derivatives and decomposi-tion products of it in the liquid while the globin lets itself be centrifuged off.
The invention i5 in the following illustrated in more detail by ... . . .
.
~: ' . , ":
: ' . ' l~SS489 some examples of execution: -EXA~LE 1 To 5 g of a hemoglobin solution with a dry substance content of 15%
and containing approx. 33% ethanol at a temperature of -8C was added in drops while stirred and cooled a mixture of 1,1 ml 1 M hydrochloric acid and 5 ml water. After the addition the temperature was -7 C and the pH value 2~5.
80 ml 94% ethanol was added in drops while cooled to -10 C. The globin was precipitated by adding 0,3 ml ammonium sulfate in a 20% water solution and centrifuged off at 8000 x g for 10 minutes. The surface liquid was a vivid brown and the paste grey. The paste was washed in 85% ethanol at a temperature of -10G and a pH-value of 3,1 and was centrifuged off, at which was obtained approx. 2,3 g paste with a dry substance content of 23%.
To a solution of 36 ml 94% ethanol, 6 ml water and 0,55 ml 1 M
hydrochloric acid at a temperature of -12 C was while stirred and cooled added in drops 2~5 g of a hemoglobin solution (same data as in example 1).
After the addition the temperature was -12 C and the p~ value 3,0.
Then 45 ml 94% ethanol was added and the liquid was centri~u~d at 8000 x g for 10 minutes~ at which was obtained a paste of an amount less than 0~1 g.
The globin was precipitated by adding 0~4 ml of a 20% ammonium sulfate solution. The pH was adjusted to 3,0 with hydrochloric acid. The precipitate was centrifuged off in the way mentioned in example 1, at which was obtained 0~45 g paste with a dry substance content of 36%.
From the supernatant was precipitated further material by adding 0,4 ml ammonium sulfate, at which 0,40 g paste with a dry substanoe content of 42% was obtain~d.
The pastss were light grey in colour. The supernatant was a vivid brown colour and gave no precipitate with trichloracetic acid.
,, ,, . , ~
~L~)55~89 To a solution of 20 ml 94% ethanol and 1,1 ml 1 M hydrochloric aaid with a temperature of -15 C was while stirred and cooled added in drops 5 g of a hemoglobin solution (same data as in example 1). After the addition the temperature was -15C and the pH value 2,9.
The mixture was centrifuged at 27000 x g for 20 minutes, at which was obtained a black paste of an amount of 0,9 g with a dry substance content of 8.3%.
To the vivid brown supernatant was while stirred and cooled added `
50 ml 94% ethanol at a temperature of ~15C and the material was precipitated by adding 10 ml water and 1,2 ml 20% ammonium sulfate.
The precipitate was centrifuged at 8000 x g for 10 minutes and the paste obtained was sludged in 94% ekhanol with a temperature of -15 ~ and ~ -centrifuged off as before. This gave 1,6 g paste with a dry substance content of 37,6%. When dry the paste was light grey.
The supernatant from the precipi~ation gave no precipitate with tri-chloracetic acid.
I
To a solution of 40 ml 94% ethanol and 1,1 ml lM hydroahloric acid with a temperature of -15C was while stirred and cooled added in drops 5 g of a hemoglobin solution (same data as in example 1). After the addition the temperature was -15 C and the p~ value 2,9.
To the mixture was added in drops 5 ml 20% sodium chloride and the liquid was centrifuged at 27000 x g for 20 minutes, at which was obtained 1,4 g black paste with a dry substance content of 11%.
To the supernatant was added 12 ml water and 1 ml of a 20% ammonium sulfate solution, after which the liquid was centrifuged at 8000 x g for 10 minutes. A grey pa~te was then obtained of an amount of 3,8 g and with a dry substance content of 14,6%.
., :
~: , ~)S~i489 According to this example an extraction was performed of the iron component from a globin precipitate.
To S g of a hemoglobin solution~(same data as in example 1~ with a temperature of -8 C was while stirred and cooled added in drops a mixture of 11 ml water, 4 ml ethanol and 1 ml 1 M hydrochloric acid with a temperature of -6C. After the addition the temperature was -4 C and the pH value 2,9.
m e pH value was adjusted to 3,9 with 7 ml of a 0,1 ~ sodium hydroxide solution. The precipitation was done by adding 0,2 g ammoni~m sulfate and the vividly brown paste was centrifuged off at approx. 300 x g. The fluid paste was sludged repeatedly in small amcunts of 94% ethanol with the pH at 3 and a temperature of -12 C and was centrifuged off. ; ~ -~his gave 3,05~ g grey paste with a dry substance content of 18,8%.
E~UMPLE 6 From 5 g of a hemoglobin solution (same data as in example l) the hemoglobin was precipitated by adding 10 ml 94% ethanol while stirred and cooled to a temperature of -6 C. The pre~ipitate was centrifuged off at 2000 x g for 5 minutes~ at which was obtained 6 g red paste. In the bottom of the cen*rifuge tube there was a deposit of a small amount of black paste. ~
The red paste was added to a solution of 70 ml 94% ethanol and 1,1 - -ml 1 M hydrochloric acid with a temperature of -12 C while stirred and cooled.
After the addition the pH value was 2,9 and the temperature ~12 C. The mix-.
ture was centrifuged at 27000 x g for 10 minutes, at which a small amount of black paste was obtained.
To the supernatant was added 2,0 ml of a ~0% ammonium sulfate solu-tion and 10 ml water. The temperature was -8 C and the pH value 3~4.
The grey precipitate was centrifuged off at 8000 x g for 10 minutes, at which ~1as obtai~d 1,5 g grey paste with a dry substance contGnt of 93,3%.
~ 5 --~5~4~
EXA~PLE 7 To a solution of 20 ml 94% ethanol and l,l ml l M hydrochloric acid with a temperature of -12 C was while stirred and cooled added in drops 5 g of a hemoglobin solution (same data as in example 1). ~fter the addition the pH value was 2,9 and the temperature -12C. The mixture, which was brownish black in colour, was centrifuged at 27000 x g for lO minutes, at which was obtained 2~2 g black paste with a dry substance content of 2,7%
and a light brown surface liquid, which later assumed a gel-like consistence.
To the supernatant was while stirred and cooled added in drops 70 10 ml 94% cold ethanol and then o,8 ml of a 40% ammonium sulfate solution and 8 ml water. The temperature was -6C after the addition.
The precipitate was centrifuged off at 8000 x g for lO minutes~ at which was obtained 1,7 g white paste with a dry substance content of 36%.
,. ... .
... ... . . .. . . .
:, . . :.
, ' ' ,: ' , , ' .
. . . ..
There are already known ways of separating, on a laboratory scale, the iron component and the globin chromatographically or by ex~raction, in which solvents like methylethyl ketone, acetone and dimethyl formamide (phor-mamide~ are used.
The chromatographic procedure is less suitable on a technical scale;
and the extraction with solvents yields a product which is unsuitable for use in feeds or foodstuffs.
In the present invention, the employment of a simple process tech-nique is made possible by uslng an organic solvent with dehydrating properties and preferably by adding a medium with an ion-strength-increasing and/or de-hydrating effect, for example, sodium chloride, sodium sulfate or ammonium sulfate, sodium benzoate or polyglycol with a possible addition of pH-regul-ating media like nonorganic acids, e.g. hydrochloric acid and sulphuric acid9 ~-or organic acids, e.g. acetic acid or citric acid. Consequently ~he invention constitutes a technically and economically practicable method for separating the iron component from the globin permitting the use of the globin in feeds and foodstuffs.
Thus, the invention provides a method for separating the heme iron component from the globin in a liquid containing blood substances, comprising cooling the liquid, adding to the liquid an organic solvent selected from the group consisting of ethanol, methanol, mixtures of ethanol and glycerol and mixtures of methanol ~nd glycerol in an amount of ~0 to 90 per cent by volume based on the total amount of khe liquid, adjusting the p~l to a value belo~
4.S, separating the heme iron component agglomerate, and precipitating the globin from the liquid. The liquid is preferably cooled to a temperature of from 0C to -20JC and ~he pH of the liquid is preferably adjusted ~o a value of from 2.5 to ~.5. ~e amount of organic solvent is preferably a~ least 75 per cent by volume, based on the total amount Oe liquid, while the iron component o~s precipitated and separated from said liquid preferably by addi-1055~39 tion of salts, such as potassium chloride or sodium citrate, whereafter theglobin is precipitated from the liquid.
According to a special form of execution of the way in accordance with the invention part of the iron component is first separated, e.g. exist-ing as an aggregate in concentrated solutions or adsorbed on protein material, preferably by centrifugation. The agglomeration can be effected by adding for instance sodium chloride. From the supernatant thus obtained the globin in then precipitated by adding ion-strength-increasing and/or dehydrating media while the iron component is retained in the liquid phase in a fluid and/or finely dispersed form.
Thus in a further embodi~ent, the method provided above is character-ized in that the organic solvent is added in two stages, a small amount of the solvent being added in a first stage preferably by adding sodium chloride or another substance that favours agglomeration and/or absorption of the iron component particles on the protein material, preferably denaturated, after which the agglomerate obtained is separated, e.g. by centrifugation, and then further organic solvent is added in a second stage for separation of the globin ~ ~ -from the remaining part of the iron component, which then is in a solved and/
or finely dispersed form.
The environment created by the high content of solvent, e.g. ethanol, and the low content of water with a certain ion strength and a low pH-value is evidently suitable for retaining the finely dispersed and the fluid form respectively of the iron component and the possible derivatives and decomposi-tion products of it in the liquid while the globin lets itself be centrifuged off.
The invention i5 in the following illustrated in more detail by ... . . .
.
~: ' . , ":
: ' . ' l~SS489 some examples of execution: -EXA~LE 1 To 5 g of a hemoglobin solution with a dry substance content of 15%
and containing approx. 33% ethanol at a temperature of -8C was added in drops while stirred and cooled a mixture of 1,1 ml 1 M hydrochloric acid and 5 ml water. After the addition the temperature was -7 C and the pH value 2~5.
80 ml 94% ethanol was added in drops while cooled to -10 C. The globin was precipitated by adding 0,3 ml ammonium sulfate in a 20% water solution and centrifuged off at 8000 x g for 10 minutes. The surface liquid was a vivid brown and the paste grey. The paste was washed in 85% ethanol at a temperature of -10G and a pH-value of 3,1 and was centrifuged off, at which was obtained approx. 2,3 g paste with a dry substance content of 23%.
To a solution of 36 ml 94% ethanol, 6 ml water and 0,55 ml 1 M
hydrochloric acid at a temperature of -12 C was while stirred and cooled added in drops 2~5 g of a hemoglobin solution (same data as in example 1).
After the addition the temperature was -12 C and the p~ value 3,0.
Then 45 ml 94% ethanol was added and the liquid was centri~u~d at 8000 x g for 10 minutes~ at which was obtained a paste of an amount less than 0~1 g.
The globin was precipitated by adding 0~4 ml of a 20% ammonium sulfate solution. The pH was adjusted to 3,0 with hydrochloric acid. The precipitate was centrifuged off in the way mentioned in example 1, at which was obtained 0~45 g paste with a dry substance content of 36%.
From the supernatant was precipitated further material by adding 0,4 ml ammonium sulfate, at which 0,40 g paste with a dry substanoe content of 42% was obtain~d.
The pastss were light grey in colour. The supernatant was a vivid brown colour and gave no precipitate with trichloracetic acid.
,, ,, . , ~
~L~)55~89 To a solution of 20 ml 94% ethanol and 1,1 ml 1 M hydrochloric aaid with a temperature of -15 C was while stirred and cooled added in drops 5 g of a hemoglobin solution (same data as in example 1). After the addition the temperature was -15C and the pH value 2,9.
The mixture was centrifuged at 27000 x g for 20 minutes, at which was obtained a black paste of an amount of 0,9 g with a dry substance content of 8.3%.
To the vivid brown supernatant was while stirred and cooled added `
50 ml 94% ethanol at a temperature of ~15C and the material was precipitated by adding 10 ml water and 1,2 ml 20% ammonium sulfate.
The precipitate was centrifuged at 8000 x g for 10 minutes and the paste obtained was sludged in 94% ekhanol with a temperature of -15 ~ and ~ -centrifuged off as before. This gave 1,6 g paste with a dry substance content of 37,6%. When dry the paste was light grey.
The supernatant from the precipi~ation gave no precipitate with tri-chloracetic acid.
I
To a solution of 40 ml 94% ethanol and 1,1 ml lM hydroahloric acid with a temperature of -15C was while stirred and cooled added in drops 5 g of a hemoglobin solution (same data as in example 1). After the addition the temperature was -15 C and the p~ value 2,9.
To the mixture was added in drops 5 ml 20% sodium chloride and the liquid was centrifuged at 27000 x g for 20 minutes, at which was obtained 1,4 g black paste with a dry substance content of 11%.
To the supernatant was added 12 ml water and 1 ml of a 20% ammonium sulfate solution, after which the liquid was centrifuged at 8000 x g for 10 minutes. A grey pa~te was then obtained of an amount of 3,8 g and with a dry substance content of 14,6%.
., :
~: , ~)S~i489 According to this example an extraction was performed of the iron component from a globin precipitate.
To S g of a hemoglobin solution~(same data as in example 1~ with a temperature of -8 C was while stirred and cooled added in drops a mixture of 11 ml water, 4 ml ethanol and 1 ml 1 M hydrochloric acid with a temperature of -6C. After the addition the temperature was -4 C and the pH value 2,9.
m e pH value was adjusted to 3,9 with 7 ml of a 0,1 ~ sodium hydroxide solution. The precipitation was done by adding 0,2 g ammoni~m sulfate and the vividly brown paste was centrifuged off at approx. 300 x g. The fluid paste was sludged repeatedly in small amcunts of 94% ethanol with the pH at 3 and a temperature of -12 C and was centrifuged off. ; ~ -~his gave 3,05~ g grey paste with a dry substance content of 18,8%.
E~UMPLE 6 From 5 g of a hemoglobin solution (same data as in example l) the hemoglobin was precipitated by adding 10 ml 94% ethanol while stirred and cooled to a temperature of -6 C. The pre~ipitate was centrifuged off at 2000 x g for 5 minutes~ at which was obtained 6 g red paste. In the bottom of the cen*rifuge tube there was a deposit of a small amount of black paste. ~
The red paste was added to a solution of 70 ml 94% ethanol and 1,1 - -ml 1 M hydrochloric acid with a temperature of -12 C while stirred and cooled.
After the addition the pH value was 2,9 and the temperature ~12 C. The mix-.
ture was centrifuged at 27000 x g for 10 minutes, at which a small amount of black paste was obtained.
To the supernatant was added 2,0 ml of a ~0% ammonium sulfate solu-tion and 10 ml water. The temperature was -8 C and the pH value 3~4.
The grey precipitate was centrifuged off at 8000 x g for 10 minutes, at which ~1as obtai~d 1,5 g grey paste with a dry substance contGnt of 93,3%.
~ 5 --~5~4~
EXA~PLE 7 To a solution of 20 ml 94% ethanol and l,l ml l M hydrochloric acid with a temperature of -12 C was while stirred and cooled added in drops 5 g of a hemoglobin solution (same data as in example 1). ~fter the addition the pH value was 2,9 and the temperature -12C. The mixture, which was brownish black in colour, was centrifuged at 27000 x g for lO minutes, at which was obtained 2~2 g black paste with a dry substance content of 2,7%
and a light brown surface liquid, which later assumed a gel-like consistence.
To the supernatant was while stirred and cooled added in drops 70 10 ml 94% cold ethanol and then o,8 ml of a 40% ammonium sulfate solution and 8 ml water. The temperature was -6C after the addition.
The precipitate was centrifuged off at 8000 x g for lO minutes~ at which was obtained 1,7 g white paste with a dry substance content of 36%.
,. ... .
... ... . . .. . . .
:, . . :.
, ' ' ,: ' , , ' .
. . . ..
Claims (10)
PROPERTY OR PRIVILEGE IS CLAIMED ARE DEFINED AS FOLLOWS:
1. A method for separating the heme iron component from the globin in a liquid containing blood substances comprising cooling the liquid, adding to the liquid an organic solvent selected from the group consisting of ethanol, methanol, mixtures of ethanol and glycerol and mixtures of methanol and gly-cerol in an amount of 40 to 90 per cent by volume based on the total amount of the liquid, adjusting the pH to a value below 4.5, separating the heme iron component agglomerate, and precipitating the globin from the liquid.
2. A method according to claim 1 in which the iron component agglomer-ation is accentuated by the addition of a salt.
3. A method according to claim 2 in which the salt is sodium chloride, potassium chloride, or sodium citrate.
4. A method according to claim 1 wherein the heme iron component is adsorbed on a protein material and separated from the liquid prior to precipi-tating the globin.
5. A method according to claim 1, 2 or 3 in which the liquid is cooled to a temperature in the range 0°C to -20°C prior to the addition of the organic solvent.
6. A method according to claim 1, 2 or 3 in which the pH is adjusted to a value in the range of 2.5 to 4.5.
7. A method according to claim 1, 2 or 3 in which the organic solvent is added in an amount of at least 75 per cent by volume.
8. A method according to claim 1, 2 or 3, wherein the precipitated glo-bin is separated from the liquid by centrifugation.
9. A method according to claim 1, wherein a further organic solvent or mixture of organic solvents is added after the separation of the heme iron component agglomerate, to effect separation of the globin from the remaining part of the heme iron component in a dissolved and/or finely dispersed form.
10. A method according to claim 8 wherein the globin is precipitated by adding to the supernatant liquid an ion-strength increasing medium selected from sodium chloride, sodium sulphate, ammonium sulphate, sodium benzoate and poly-glycol.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE7407882-5A SE389596B (en) | 1974-06-14 | 1974-06-14 | SUGGESTED TO SEPARATE GLOBIN IRON COMPOUNDS FROM A VETICAL CONTAINING BLOOD SUBSTANCES |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1055489A true CA1055489A (en) | 1979-05-29 |
Family
ID=20321445
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA229,318A Expired CA1055489A (en) | 1974-06-14 | 1975-06-13 | Method of separating heme iron compounds from globin |
Country Status (18)
| Country | Link |
|---|---|
| AR (1) | AR209770A1 (en) |
| AT (1) | AT346672B (en) |
| AU (1) | AU502954B2 (en) |
| BR (1) | BR7503722A (en) |
| CA (1) | CA1055489A (en) |
| DE (1) | DE2526596C2 (en) |
| DK (1) | DK150173B (en) |
| ES (1) | ES438483A1 (en) |
| FI (1) | FI59195C (en) |
| FR (1) | FR2274230A1 (en) |
| HU (1) | HU174189B (en) |
| IE (1) | IE49166B1 (en) |
| PL (1) | PL94776B1 (en) |
| RO (1) | RO63791A (en) |
| SE (1) | SE389596B (en) |
| SU (1) | SU910135A3 (en) |
| YU (1) | YU147475A (en) |
| ZA (1) | ZA753806B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4761472A (en) * | 1986-03-12 | 1988-08-02 | Basf Aktiengesellschaft | Isolation and purification of hemin |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1126653A (en) * | 1978-12-22 | 1982-06-29 | Jan H. Luijerink | Process of preparing blood cell protein and heme from hemoglobin |
| SE440596B (en) * | 1980-04-03 | 1985-08-12 | Paul Goran Sigvard Lindroos | PROCEDURE FOR PREPARING A HOME CONCENTRATE FROM A MIXTURE OF HOME AND BLOOD SUBSTANCE RECOVERY BY DIVISION OF HEMOGLOBIN |
| FR2535173A1 (en) * | 1982-11-03 | 1984-05-04 | Protein Sa | Products obtained from the blood of abattoir animals and method for obtaining them. |
| JPS59128337A (en) * | 1983-01-11 | 1984-07-24 | Riyoushiyoku Kenkyukai | Recovery of blood globin and haem |
| FR2548671B1 (en) * | 1983-07-07 | 1986-05-02 | Merieux Inst | PROCESS FOR THE PREPARATION OF A GLOBIN FROM HEMOGLOBIN AND A GLOBIN OBTAINED BY THIS PROCESS |
| WO1990005461A1 (en) * | 1988-11-18 | 1990-05-31 | Tosoh Corporation | Process for purifying blood plasma |
| ES2181046T3 (en) * | 1996-12-20 | 2003-02-16 | Fraunhofer Ges Forschung | PROCEDURE FOR THE OBTAINING OF FEMALE FROM BLOOD OF KILLING. |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE178902C (en) * | 1905-10-16 | |||
| SE348942B (en) * | 1970-06-02 | 1972-09-18 | Statens Bakteriologiska Labor |
-
1974
- 1974-06-14 SE SE7407882-5A patent/SE389596B/en not_active IP Right Cessation
-
1975
- 1975-05-29 AU AU81668/75A patent/AU502954B2/en not_active Expired
- 1975-06-04 AR AR259065A patent/AR209770A1/en active
- 1975-06-04 FI FI751643A patent/FI59195C/en not_active IP Right Cessation
- 1975-06-06 YU YU01474/75A patent/YU147475A/en unknown
- 1975-06-12 HU HU75LI279A patent/HU174189B/en unknown
- 1975-06-12 FR FR7518352A patent/FR2274230A1/en active Granted
- 1975-06-12 ES ES438483A patent/ES438483A1/en not_active Expired
- 1975-06-12 BR BR4779/75D patent/BR7503722A/en unknown
- 1975-06-12 PL PL1975181157A patent/PL94776B1/en unknown
- 1975-06-13 DK DK266675AA patent/DK150173B/en not_active Application Discontinuation
- 1975-06-13 AT AT456575A patent/AT346672B/en not_active IP Right Cessation
- 1975-06-13 IE IE1335/75A patent/IE49166B1/en unknown
- 1975-06-13 SU SU752143704A patent/SU910135A3/en active
- 1975-06-13 DE DE2526596A patent/DE2526596C2/en not_active Expired
- 1975-06-13 CA CA229,318A patent/CA1055489A/en not_active Expired
- 1975-06-13 ZA ZA3806A patent/ZA753806B/en unknown
- 1975-06-14 RO RO7500082550A patent/RO63791A/en unknown
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4761472A (en) * | 1986-03-12 | 1988-08-02 | Basf Aktiengesellschaft | Isolation and purification of hemin |
Also Published As
| Publication number | Publication date |
|---|---|
| ZA753806B (en) | 1977-01-26 |
| AU502954B2 (en) | 1979-08-16 |
| IE49166B1 (en) | 1985-08-21 |
| DK150173B (en) | 1986-12-29 |
| FI751643A (en) | 1975-12-15 |
| BR7503722A (en) | 1976-06-29 |
| ATA456575A (en) | 1978-03-15 |
| SU910135A3 (en) | 1982-02-28 |
| FI59195C (en) | 1981-07-10 |
| AU8166875A (en) | 1976-12-02 |
| DE2526596C2 (en) | 1984-11-29 |
| PL94776B1 (en) | 1977-08-31 |
| FI59195B (en) | 1981-03-31 |
| DE2526596A1 (en) | 1976-01-02 |
| AT346672B (en) | 1978-11-27 |
| YU147475A (en) | 1982-05-31 |
| ES438483A1 (en) | 1977-02-01 |
| SE7407882L (en) | 1975-12-15 |
| FR2274230B1 (en) | 1981-03-27 |
| RO63791A (en) | 1978-12-15 |
| FR2274230A1 (en) | 1976-01-09 |
| DK266675A (en) | 1975-12-15 |
| HU174189B (en) | 1979-11-28 |
| AR209770A1 (en) | 1977-05-31 |
| SE389596B (en) | 1976-11-15 |
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