CA1054085A - Urokinase production - Google Patents
Urokinase productionInfo
- Publication number
- CA1054085A CA1054085A CA236,800A CA236800A CA1054085A CA 1054085 A CA1054085 A CA 1054085A CA 236800 A CA236800 A CA 236800A CA 1054085 A CA1054085 A CA 1054085A
- Authority
- CA
- Canada
- Prior art keywords
- urokinase
- days
- medium
- glycine
- production
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 102000003990 Urokinase-type plasminogen activator Human genes 0.000 title claims abstract description 34
- 108090000435 Urokinase-type plasminogen activator Proteins 0.000 title claims abstract description 34
- 229960005356 urokinase Drugs 0.000 title claims abstract description 34
- 238000004519 manufacturing process Methods 0.000 title abstract description 22
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims abstract description 62
- 239000004471 Glycine Substances 0.000 claims abstract description 24
- 235000015097 nutrients Nutrition 0.000 claims description 10
- 238000004113 cell culture Methods 0.000 claims description 8
- 210000003292 kidney cell Anatomy 0.000 claims description 8
- 238000012423 maintenance Methods 0.000 claims description 7
- 239000000654 additive Substances 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 4
- 239000013587 production medium Substances 0.000 abstract description 3
- 239000002609 medium Substances 0.000 description 30
- 210000004027 cell Anatomy 0.000 description 15
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 8
- 150000001413 amino acids Chemical class 0.000 description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 5
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 4
- 229910002092 carbon dioxide Inorganic materials 0.000 description 4
- 239000001569 carbon dioxide Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 208000007536 Thrombosis Diseases 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000010261 cell growth Effects 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 230000000644 propagated effect Effects 0.000 description 2
- 235000017557 sodium bicarbonate Nutrition 0.000 description 2
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- BVKZGUZCCUSVTD-UHFFFAOYSA-M Bicarbonate Chemical compound OC([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-M 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 101710196208 Fibrinolytic enzyme Proteins 0.000 description 1
- 108091006905 Human Serum Albumin Proteins 0.000 description 1
- 102000008100 Human Serum Albumin Human genes 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 102000013566 Plasminogen Human genes 0.000 description 1
- 108010051456 Plasminogen Proteins 0.000 description 1
- 108010009736 Protein Hydrolysates Proteins 0.000 description 1
- 241000220317 Rosa Species 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 235000005550 amino acid supplement Nutrition 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012894 fetal calf serum Substances 0.000 description 1
- JCYWCSGERIELPG-UHFFFAOYSA-N imes Chemical class CC1=CC(C)=CC(C)=C1N1C=CN(C=2C(=CC(C)=CC=2C)C)[C]1 JCYWCSGERIELPG-UHFFFAOYSA-N 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 239000010410 layer Substances 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 229940012957 plasmin Drugs 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 230000002485 urinary effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
- C12N9/6462—Plasminogen activators u-Plasminogen activator (3.4.21.73), i.e. urokinase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21073—Serine endopeptidases (3.4.21) u-Plasminogen activator (3.4.21.73), i.e. urokinase
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US05/564,966 US3930945A (en) | 1975-03-31 | 1975-03-31 | Urokinase production |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1054085A true CA1054085A (en) | 1979-05-08 |
Family
ID=24256647
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA236,800A Expired CA1054085A (en) | 1975-03-31 | 1975-10-01 | Urokinase production |
Country Status (15)
| Country | Link |
|---|---|
| US (1) | US3930945A (OSRAM) |
| JP (1) | JPS51128490A (OSRAM) |
| AR (1) | AR208001A1 (OSRAM) |
| BE (1) | BE835023A (OSRAM) |
| CA (1) | CA1054085A (OSRAM) |
| CH (1) | CH601477A5 (OSRAM) |
| DE (1) | DE2551017C3 (OSRAM) |
| DK (1) | DK140561B (OSRAM) |
| FR (1) | FR2306263A1 (OSRAM) |
| GB (1) | GB1491816A (OSRAM) |
| MX (1) | MX3022E (OSRAM) |
| NL (1) | NL164898C (OSRAM) |
| PH (1) | PH11173A (OSRAM) |
| SE (1) | SE415035B (OSRAM) |
| ZA (1) | ZA756194B (OSRAM) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4190708A (en) * | 1978-05-24 | 1980-02-26 | Monsanto Company | Production of urokinase |
| JPS5571496A (en) * | 1978-11-24 | 1980-05-29 | Asahi Chem Ind Co Ltd | Preparation of living substance |
| US4232124A (en) * | 1979-04-23 | 1980-11-04 | Mann George F | Method of producing plasminogen activator |
| DE3015699C2 (de) * | 1979-04-26 | 1982-07-15 | Asahi Kasei Kogyo K.K., Osaka | Herstellung eines Plasminogen-Aktivators |
| JPS5852634B2 (ja) * | 1980-12-05 | 1983-11-24 | 株式会社林原生物化学研究所 | ウロキナ−ゼの製造法 |
| DE3584902D1 (de) * | 1984-02-29 | 1992-01-30 | Asahi Chemical Ind | Waessrige loesung eines darin in erhoehter konzentration aufgeloesten gewebe-plasminogen-aktivators und herstellungsverfahren. |
| US4661453A (en) * | 1984-06-19 | 1987-04-28 | American Biogenetic Sciences, Inc. | Production of tissue plasminogen activator factor |
| JPS61207335A (ja) * | 1985-03-12 | 1986-09-13 | Kouchi Ika Univ | 悪性リンパ腫細胞株ｒｃ−ｋ８の産生するプラスミノゲン・アクチベ−タ− |
| US4920051A (en) * | 1988-02-03 | 1990-04-24 | Damon Biotech, Inc. | Recovery of urokinase compounds |
-
1975
- 1975-01-01 AR AR261024A patent/AR208001A1/es active
- 1975-03-31 US US05/564,966 patent/US3930945A/en not_active Expired - Lifetime
- 1975-09-30 ZA ZA00756194A patent/ZA756194B/xx unknown
- 1975-10-01 CA CA236,800A patent/CA1054085A/en not_active Expired
- 1975-10-02 MX MX006395U patent/MX3022E/es unknown
- 1975-10-06 GB GB40878/75A patent/GB1491816A/en not_active Expired
- 1975-10-09 NL NL7511872.A patent/NL164898C/xx not_active IP Right Cessation
- 1975-10-30 BE BE2054638A patent/BE835023A/xx not_active IP Right Cessation
- 1975-10-31 JP JP50130577A patent/JPS51128490A/ja active Granted
- 1975-11-10 CH CH1451175A patent/CH601477A5/xx not_active IP Right Cessation
- 1975-11-10 FR FR7534342A patent/FR2306263A1/fr active Granted
- 1975-11-11 PH PH17759A patent/PH11173A/en unknown
- 1975-11-13 SE SE7512782A patent/SE415035B/xx unknown
- 1975-11-13 DE DE2551017A patent/DE2551017C3/de not_active Expired
- 1975-11-14 DK DK515375AA patent/DK140561B/da not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| FR2306263B1 (OSRAM) | 1979-06-01 |
| BE835023A (fr) | 1976-04-30 |
| AU8545175A (en) | 1977-04-07 |
| JPS5414190B2 (OSRAM) | 1979-06-05 |
| DK140561C (OSRAM) | 1980-03-17 |
| DK140561B (da) | 1979-10-01 |
| NL7511872A (nl) | 1976-10-04 |
| US3930945A (en) | 1976-01-06 |
| DE2551017B2 (de) | 1978-01-19 |
| FR2306263A1 (fr) | 1976-10-29 |
| ZA756194B (en) | 1976-09-29 |
| DE2551017C3 (de) | 1978-09-21 |
| SE7512782L (sv) | 1976-10-01 |
| JPS51128490A (en) | 1976-11-09 |
| CH601477A5 (OSRAM) | 1978-07-14 |
| NL164898C (nl) | 1981-02-16 |
| SE415035B (sv) | 1980-09-01 |
| MX3022E (es) | 1980-02-13 |
| DK515375A (OSRAM) | 1976-10-01 |
| NL164898B (nl) | 1980-09-15 |
| DE2551017A1 (de) | 1976-10-07 |
| PH11173A (en) | 1977-10-28 |
| GB1491816A (en) | 1977-11-16 |
| AR208001A1 (es) | 1976-11-22 |
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