BRPI0910821A8 - Método para modificar uma proteína, alimento, preparação de enzima para modificar uma proteína, e, método para estabelecer uma relação ótima de proteína glutaminase para transglutaminase - Google Patents

Método para modificar uma proteína, alimento, preparação de enzima para modificar uma proteína, e, método para estabelecer uma relação ótima de proteína glutaminase para transglutaminase

Info

Publication number
BRPI0910821A8
BRPI0910821A8 BRPI0910821A BRPI0910821A BRPI0910821A8 BR PI0910821 A8 BRPI0910821 A8 BR PI0910821A8 BR PI0910821 A BRPI0910821 A BR PI0910821A BR PI0910821 A BRPI0910821 A BR PI0910821A BR PI0910821 A8 BRPI0910821 A8 BR PI0910821A8
Authority
BR
Brazil
Prior art keywords
protein
transglutaminase
glutaminase
modifying
establishing
Prior art date
Application number
BRPI0910821A
Other languages
English (en)
Inventor
Nakamura Mina
Suzuki Eiichiro
Yokoyama Keiichi
Nakagoshi Hiroyuki
Hirose Fumiyuki
Sato Hiroaki
Miwa Noriko
Shimba Nobuhisa
Original Assignee
Ajinomoto Kk
Amano Enzyme Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ajinomoto Kk, Amano Enzyme Inc filed Critical Ajinomoto Kk
Publication of BRPI0910821A2 publication Critical patent/BRPI0910821A2/pt
Publication of BRPI0910821A8 publication Critical patent/BRPI0910821A8/pt
Publication of BRPI0910821B1 publication Critical patent/BRPI0910821B1/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1096—Transferases (2.) transferring nitrogenous groups (2.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/52—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving transaminase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/91045—Acyltransferases (2.3)
    • G01N2333/91074—Aminoacyltransferases (general) (2.3.2)
    • G01N2333/9108—Aminoacyltransferases (general) (2.3.2) with definite EC number (2.3.2.-)
    • G01N2333/91085—Transglutaminases; Factor XIIIq (2.3.2.13)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Dairy Products (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • General Preparation And Processing Of Foods (AREA)

Abstract

MÉTODO PARA MODIFICAR UMA PROTEÍNA, ALIMENTO, PREPARAÇÃO DE ENZIMA PARA MODIFICAR UMA PROEÍNA, E, MÉTODO PARA ESTABELECER UMA RELAÇÃO ÓTIMA DE PROTEÍNA GLUTAMINASE PARA TRANSGLUTAMINASE. São providos: um método de desnaturar uma proteína por tratamento com tanto as proteínas glutaminase como transglutaminase; um alimento contendo uma proteína tendo sido desnaturada com estas enzimas; uma preparação de enzima para desnaturar uma proteína que compreende estas enzimas; e um método para estabelecer a relação quantitativa ótima de proteína glutaminase à transglutaminase todas as duas sendo adicionadas a uma proteína. Uma proteína é desnaturada por adição de proteína glutaminase e a transglutaminase à proteína substancialmente ao mesmo tempo, ou adição da proteína glutaminase à proteína antes que a transglutaminase atue sobre a proteína, ou controlando a relação quantitativa da proteína glutaminase à transglutaminase, pelo que uma proteína é tratada, em um nível definitivo. A relação quantitativa ótima de proteína glutaminase para transglutaminase, todas as duas sendo adicionadas a uma proteína, é determinada com base na intensidade do sinal de RMN da proteína rotulada com amônio rotulado por um isótopo ou semelhante.
BRPI0910821-1A 2008-03-14 2009-03-12 Método para modificar uma proteína, alimento, preparação de enzima para modificar uma proteína, e, método para estabelecer uma relação ótima de proteína glutaminase para transglutaminase BRPI0910821B1 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JP2008066765 2008-03-14
JP2008-066765 2008-03-14
JP2008-294890 2008-11-18
JP2008294890 2008-11-18
PCT/JP2009/054792 WO2009113628A1 (ja) 2008-03-14 2009-03-12 酵素によるタンパク質の改質方法

Publications (3)

Publication Number Publication Date
BRPI0910821A2 BRPI0910821A2 (pt) 2015-08-18
BRPI0910821A8 true BRPI0910821A8 (pt) 2017-08-08
BRPI0910821B1 BRPI0910821B1 (pt) 2018-07-10

Family

ID=41065288

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0910821-1A BRPI0910821B1 (pt) 2008-03-14 2009-03-12 Método para modificar uma proteína, alimento, preparação de enzima para modificar uma proteína, e, método para estabelecer uma relação ótima de proteína glutaminase para transglutaminase

Country Status (10)

Country Link
EP (1) EP2267146A4 (pt)
JP (1) JP5616216B2 (pt)
KR (1) KR101577601B1 (pt)
CN (1) CN102016057A (pt)
BR (1) BRPI0910821B1 (pt)
CA (1) CA2717340A1 (pt)
MX (1) MX2010010076A (pt)
MY (1) MY155718A (pt)
TW (1) TW200942616A (pt)
WO (1) WO2009113628A1 (pt)

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KR100846898B1 (ko) 2005-12-30 2008-07-17 엘지전자 주식회사 영상 디스플레이 기기의 스탠드
JP4856665B2 (ja) * 2008-03-14 2012-01-18 味の素株式会社 麺類の製造法
CN101861909B (zh) * 2010-05-05 2012-09-12 长沙理工大学 一种蛋白质谷氨酰胺酶对大米蛋白和米谷蛋白改性的方法
JP5719752B2 (ja) * 2011-10-28 2015-05-20 森永乳業株式会社 発酵乳の製造方法
CN104736135B (zh) * 2012-10-17 2019-07-09 味之素株式会社 化妆品组合物
CN103263688B (zh) * 2013-05-10 2014-07-30 华东师范大学 一种明胶组合物及其制备方法和应用
CN103478701B (zh) * 2013-09-24 2015-04-22 河南翔宇食品有限公司 一种无淀粉香菇素肠及其加工方法
CN104782878A (zh) * 2015-05-06 2015-07-22 东北农业大学 一种酶法改性乳清蛋白可溶性聚合物的制备方法
BR112018069455B1 (pt) 2016-03-30 2022-09-06 Ajinomoto Co., Inc. Método para produção de iogurte
CN106434799A (zh) * 2016-09-28 2017-02-22 华南理工大学 一种蛋白质纳米颗粒及其制备方法
CN106858587A (zh) * 2017-01-18 2017-06-20 华南理工大学 大豆分离蛋白‑姜黄素纳米颗粒结合物及其制备方法
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US20230183771A1 (en) * 2020-04-03 2023-06-15 Amano Enzyme Inc. Protein deamidation method
EP4162806A1 (en) * 2020-06-08 2023-04-12 Amano Enzyme Inc. Improving texture of protein
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US20220053786A1 (en) * 2020-08-19 2022-02-24 Ajinomoto Co., Inc. Methods for producing stirred yogurt
EP4201216A1 (en) * 2020-08-24 2023-06-28 Amano Enzyme Inc. Vegetable milk treated with protein deamidase
CN114304276A (zh) * 2020-09-30 2022-04-12 天野酶制品株式会社 加工植物性奶的制造方法
EP4249590A1 (en) 2020-11-20 2023-09-27 Amano Enzyme Inc. Thermotolerant protein glutaminase
WO2023085315A1 (ja) * 2021-11-09 2023-05-19 天野エンザイム株式会社 植物性タンパク質含有組成物の消化性向上剤
CN114403280B (zh) * 2022-01-30 2023-06-09 杭州佳嘉乐生物技术有限公司 一种具有多种功能的纳米级牡蛎肽粉的制备方法
CN114836403B (zh) * 2022-04-28 2023-08-25 江南大学 一种利用蛋白质谷氨酰胺酶改善糖酶催化性能的方法
WO2024053745A1 (ja) * 2022-09-09 2024-03-14 天野エンザイム株式会社 加工植物性タンパク質含有組成物の製造方法

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Also Published As

Publication number Publication date
JPWO2009113628A1 (ja) 2011-07-21
JP5616216B2 (ja) 2014-10-29
BRPI0910821B1 (pt) 2018-07-10
KR101577601B1 (ko) 2015-12-15
KR20100127824A (ko) 2010-12-06
CA2717340A1 (en) 2009-09-17
EP2267146A1 (en) 2010-12-29
BRPI0910821A2 (pt) 2015-08-18
MX2010010076A (es) 2010-10-20
EP2267146A4 (en) 2011-05-04
TW200942616A (en) 2009-10-16
MY155718A (en) 2015-11-30
CN102016057A (zh) 2011-04-13
WO2009113628A1 (ja) 2009-09-17

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B15K Others concerning applications: alteration of classification

Ipc: C12P 21/02 (2006.01), C12N 9/10 (2006.01), C12N 9/

B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]