BR9916627A - Processo para produzir transglutaminase tendoatividade enzimática, e, protéina - Google Patents

Processo para produzir transglutaminase tendoatividade enzimática, e, protéina

Info

Publication number
BR9916627A
BR9916627A BR9916627-5A BR9916627A BR9916627A BR 9916627 A BR9916627 A BR 9916627A BR 9916627 A BR9916627 A BR 9916627A BR 9916627 A BR9916627 A BR 9916627A
Authority
BR
Brazil
Prior art keywords
transglutaminase
enzymatic activity
activity
denatured
produced
Prior art date
Application number
BR9916627-5A
Other languages
English (en)
Other versions
BR9916627B1 (pt
Inventor
Keiichi Yokoyama
Kunio Ono
Daisuke Ejima
Original Assignee
Ajinomoto Kk
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ajinomoto Kk filed Critical Ajinomoto Kk
Publication of BR9916627A publication Critical patent/BR9916627A/pt
Publication of BR9916627B1 publication Critical patent/BR9916627B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/104—Aminoacyltransferases (2.3.2)
    • C12N9/1044—Protein-glutamine gamma-glutamyltransferase (2.3.2.13), i.e. transglutaminase or factor XIII
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/02—Aminoacyltransferases (2.3.2)
    • C12Y203/02013—Protein-glutamine gamma-glutamyltransferase (2.3.2.13), i.e. transglutaminase or factor XIII

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

"PROCESSO PARA PRODUZIR TRANSGLUTAMINASE TENDOATIVIDADE ENZIMáTICA, E, PROTEìNA".Transglutaminasetendo atividade enzimática é produzido ao submetertransglutaminase desnaturada a etapas contendo pelo menos asseguintes etapas (a) e (b): (a) uma etapa de formação de umaestrutura intermediária em construção estrutural para o estadotendo a atividade enzimática em que a enzima desnaturada édeixada reagir em um meio aquoso sob uma condição ácida, e (b)uma etapa para formar uma estrutura de ordem superior em que aenzima tendo a estrutura intermediária na construção estruturalacaba tendo a atividade enzimática em um meio aquoso sob umaregião de pH neutro. A transglutaminase desnaturada produzidacom um microorganismo recombinante pode ser reduplicada emboa eficiência e transglutaminase de alta pureza tendosubstancialmente a mesma atividade enzimática que atransglutaminase nativa, ou seja atividade de transglutaminase,pode ser produzida industrialmente. Um produto intermediário tendoa estrutura intermediária na construção estrutural é tambémprovido.
BRPI9916627-5A 1998-12-28 1999-12-24 processo para produzir transglutaminase tendo atividade enzimática. BR9916627B1 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP37313198 1998-12-28
PCT/JP1999/007250 WO2000040706A1 (fr) 1998-12-28 1999-12-24 Procede de production de transglutaminase

Publications (2)

Publication Number Publication Date
BR9916627A true BR9916627A (pt) 2001-10-30
BR9916627B1 BR9916627B1 (pt) 2011-05-31

Family

ID=18501629

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI9916627-5A BR9916627B1 (pt) 1998-12-28 1999-12-24 processo para produzir transglutaminase tendo atividade enzimática.

Country Status (10)

Country Link
US (1) US6833258B2 (pt)
EP (1) EP1142990B1 (pt)
JP (1) JP4239412B2 (pt)
CN (1) CN1203173C (pt)
AU (1) AU762951B2 (pt)
BR (1) BR9916627B1 (pt)
CA (1) CA2356597A1 (pt)
DE (1) DE69940891D1 (pt)
DK (1) DK1142990T3 (pt)
WO (1) WO2000040706A1 (pt)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7101695B2 (en) 2002-03-01 2006-09-05 Szu-Yi Chou Method of producing transglutaminase having broad substrate activity
US7485438B2 (en) 2002-03-01 2009-02-03 Szu-Yi Chou Method of producing polyvalent antigens
ES2223227B1 (es) * 2002-05-31 2006-04-16 Consejo Sup. Investig. Cientificas Secuencia de nucleotidos de maiz codificante de una proteina con actividad transglutaminasa, y su uso.
US20050190988A1 (en) * 2004-03-01 2005-09-01 Mass Institute Of Technology (Mit) Passive positioning sensors
WO2007014170A2 (en) * 2005-07-25 2007-02-01 Trubion Pharmaceuticals, Inc. Compositions and methods for protein deaggregation
CN101854960B (zh) 2006-12-15 2014-06-25 生命连结有限公司 明胶-转谷氨酰胺酶止血敷料和密封材料
EP2281827B1 (en) * 2008-05-08 2013-08-21 Ajinomoto Co., Inc. Protein refolding method
AU2009261559B2 (en) 2008-06-18 2014-01-16 Lifebond Ltd Improved cross-linked compositions
CN102711853B (zh) 2009-12-22 2015-05-20 生命连结有限公司 用于控制交联基质的性质的酶交联剂的改性
DK2600910T3 (en) 2010-08-05 2016-04-04 Lifebond Ltd Wound dressings and adhesives COMPREHENSIVE DRYING FORMATIONS
CN102146361B (zh) * 2011-02-01 2013-06-19 泰兴市一鸣生物制品有限公司 一种分离纯化谷氨酰胺转胺酶的方法
JP6086378B2 (ja) * 2012-11-30 2017-03-01 国立大学法人お茶の水女子大学 組み換えヒト膵臓リパーゼの調製方法
WO2014128726A2 (en) * 2013-02-22 2014-08-28 Biogenomics Limited Process for high efficiency refolding of recombinant proteins
WO2014167574A1 (en) * 2013-03-22 2014-10-16 Biogenomics Limited Process for isolation and stabilisation of key intermediates for high efficiency refolding of recombinant proteins
CN108103040B (zh) * 2018-02-02 2021-05-04 泰兴市东圣生物科技有限公司 一种耐酸性微生物转谷酰胺酶及其编码基因
US11998654B2 (en) 2018-07-12 2024-06-04 Bard Shannon Limited Securing implants and medical devices

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH01222780A (ja) * 1988-03-02 1989-09-06 Tosoh Corp ウロキナーゼ前駆体様蛋白質のリフォールディング方法
JPH01257491A (ja) * 1988-04-08 1989-10-13 Tosoh Corp 不溶性融合異種蛋白質の処理方法
JP3656277B2 (ja) 1995-05-17 2005-06-08 味の素株式会社 組換えｄｎａ法によるトランスグルタミナーゼの効率的製造法
DK0889133T3 (da) 1997-07-04 2004-04-13 Ajinomoto Kk Proces til frembringelse af mikrobiel transglutaminase

Also Published As

Publication number Publication date
EP1142990A1 (en) 2001-10-10
WO2000040706A1 (fr) 2000-07-13
EP1142990B1 (en) 2009-05-13
CN1203173C (zh) 2005-05-25
DK1142990T3 (da) 2009-07-27
JP4239412B2 (ja) 2009-03-18
CN1334867A (zh) 2002-02-06
AU762951B2 (en) 2003-07-10
US20020090675A1 (en) 2002-07-11
DE69940891D1 (de) 2009-06-25
EP1142990A4 (en) 2002-06-05
CA2356597A1 (en) 2000-07-13
BR9916627B1 (pt) 2011-05-31
US6833258B2 (en) 2004-12-21
AU1799100A (en) 2000-07-24

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Legal Events

Date Code Title Description
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 31/05/2011, OBSERVADAS AS CONDICOES LEGAIS.

B21A Patent or certificate of addition expired [chapter 21.1 patent gazette]

Free format text: PATENTE EXTINTA EM 31.05.2021