BR9911712A - Sistema para uso na produção de um policetìdeo, multi-enzima pks, ácido nucleico, vetor, organismo transformante, variante de um policetìdeo parental e processo para preparar um policetìdeo. - Google Patents

Sistema para uso na produção de um policetìdeo, multi-enzima pks, ácido nucleico, vetor, organismo transformante, variante de um policetìdeo parental e processo para preparar um policetìdeo.

Info

Publication number
BR9911712A
BR9911712A BR9911712-6A BR9911712A BR9911712A BR 9911712 A BR9911712 A BR 9911712A BR 9911712 A BR9911712 A BR 9911712A BR 9911712 A BR9911712 A BR 9911712A
Authority
BR
Brazil
Prior art keywords
polyketide
pks
enzyme
parental
polycetide
Prior art date
Application number
BR9911712-6A
Other languages
English (en)
Inventor
Peter Francis Leadlay
James Staunton
Jesus Cortes
Hamish Alastair Irvin Mcarthur
Original Assignee
Biotica Tech Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biotica Tech Ltd filed Critical Biotica Tech Ltd
Publication of BR9911712A publication Critical patent/BR9911712A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D313/00—Heterocyclic compounds containing rings of more than six members having one oxygen atom as the only ring hetero atom
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H17/00—Compounds containing heterocyclic radicals directly attached to hetero atoms of saccharide radicals
    • C07H17/04—Heterocyclic radicals containing only oxygen as ring hetero atoms
    • C07H17/08—Hetero rings containing eight or more ring members, e.g. erythromycins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02—Oxygen as only ring hetero atoms
    • C12P17/06—Oxygen as only ring hetero atoms containing a six-membered hetero ring, e.g. fluorescein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/60—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin
    • C12P19/62—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin the hetero ring having eight or more ring members and only oxygen as ring hetero atoms, e.g. erythromycin, spiramycin, nystatin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Communicable Diseases (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Oncology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Polymers With Sulfur, Phosphorus Or Metals In The Main Chain (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Polyesters Or Polycarbonates (AREA)
  • Plural Heterocyclic Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Polyoxymethylene Polymers And Polymers With Carbon-To-Carbon Bonds (AREA)
  • Catalysts (AREA)
  • Peptides Or Proteins (AREA)

Abstract

<B>''SISTEMA PARA USO NA PRODUçãO DE UM POLICETìDEO, MULTI-ENZIMA PKS, áCIDO NUCLEICO, VETOR, ORGANISMO TRANSFORMANTE, VARIANTE DE UM POLICETìDEO PARENTAL E PROCESSO PARA PREPARAR UM POLICETìDEO''<D> Uma policetídeo sintase (''PKS") de tipo I é uma multi-enzima complexa incluindo um domínio de carga ligado a uma multiplicidade de domínios de extensão. O primeiro módulo de extensão recebe uma unidade iniciadora de acila do domínio de carga e cada módulo de extensão adiciona uma outra unidade de cetídeo que pode sofrer processamento (por exemplo redução). Os requerentes verificaram que o domínio Ksq possuído por alguns dos PKS's tem atividade descarboxilante, por exemplo gerando acila (substituída) a partir de malonila (substituída). O domínio CLF de PKS's de tipo II tem atividade similar. Por inserção de módulos de carga incluindo estes domínios em PKS's não normalmente possuindo os mesmos, é possível controlar as unidades iniciadoras usadas.
BR9911712-6A 1998-06-29 1999-06-29 Sistema para uso na produção de um policetìdeo, multi-enzima pks, ácido nucleico, vetor, organismo transformante, variante de um policetìdeo parental e processo para preparar um policetìdeo. BR9911712A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9814006.4A GB9814006D0 (en) 1998-06-29 1998-06-29 Polyketides and their synthesis
PCT/GB1999/002044 WO2000000618A2 (en) 1998-06-29 1999-06-29 Polyketides and their synthesis

Publications (1)

Publication Number Publication Date
BR9911712A true BR9911712A (pt) 2001-03-20

Family

ID=10834580

Family Applications (2)

Application Number Title Priority Date Filing Date
BR9911710-0A BR9911710A (pt) 1998-06-29 1999-06-29 Macrolìdeo de 14 membros, composto, processo para produzir um composto, sistema para produzir macrolìdeo de 14 membros, multi-enzima pks, ácido nucleico, vetor, e, organismo transformante.
BR9911712-6A BR9911712A (pt) 1998-06-29 1999-06-29 Sistema para uso na produção de um policetìdeo, multi-enzima pks, ácido nucleico, vetor, organismo transformante, variante de um policetìdeo parental e processo para preparar um policetìdeo.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
BR9911710-0A BR9911710A (pt) 1998-06-29 1999-06-29 Macrolìdeo de 14 membros, composto, processo para produzir um composto, sistema para produzir macrolìdeo de 14 membros, multi-enzima pks, ácido nucleico, vetor, e, organismo transformante.

Country Status (23)

Country Link
US (3) US7148045B1 (pt)
EP (2) EP1091971B1 (pt)
JP (2) JP4489947B2 (pt)
KR (2) KR20010071684A (pt)
CN (2) CN1316001A (pt)
AT (2) ATE461289T1 (pt)
AU (2) AU763230B2 (pt)
BR (2) BR9911710A (pt)
CA (2) CA2335523A1 (pt)
CZ (2) CZ20004913A3 (pt)
DE (2) DE69942149D1 (pt)
ES (1) ES2341403T3 (pt)
GB (1) GB9814006D0 (pt)
HK (1) HK1034536A1 (pt)
HU (2) HUP0105179A2 (pt)
ID (2) ID27419A (pt)
IL (2) IL140440A0 (pt)
MX (2) MXPA00012885A (pt)
NZ (2) NZ509601A (pt)
PL (2) PL345575A1 (pt)
TR (2) TR200003771T2 (pt)
WO (2) WO2000000500A2 (pt)
YU (2) YU82500A (pt)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7738015B2 (en) * 1997-10-09 2010-06-15 Fotonation Vision Limited Red-eye filter method and apparatus
US7630006B2 (en) * 1997-10-09 2009-12-08 Fotonation Ireland Limited Detecting red eye filter and apparatus using meta-data
GB9814622D0 (en) * 1998-07-06 1998-09-02 Biotica Tech Ltd Polyketides,their preparation,and materials for use therein
US6590083B1 (en) 1999-04-16 2003-07-08 Ortho-Mcneil Pharmaceutical, Inc. Ketolide antibacterials
JP2002542197A (ja) 1999-04-16 2002-12-10 オーソ−マクニール・フアーマシユーチカル・インコーポレーテツド ケトライド抗菌剤
GB9912563D0 (en) * 1999-05-28 1999-07-28 Biotica Tech Ltd Polyketides and their synthesis
US6524841B1 (en) * 1999-10-08 2003-02-25 Kosan Biosciences, Inc. Recombinant megalomicin biosynthetic genes and uses thereof
US7033818B2 (en) 1999-10-08 2006-04-25 Kosan Biosciences, Inc. Recombinant polyketide synthase genes
ATE453719T1 (de) 2000-04-13 2010-01-15 Biotica Tech Ltd Glycosylierte hybrid-verbindungen, deren herstellung und verwendung
KR101016561B1 (ko) * 2002-02-19 2011-02-22 다우 아그로사이언시즈 엘엘씨 신규한 스피노신 생산 폴리케타이드 신타제
EP1967520A2 (en) 2002-07-16 2008-09-10 Biotica Technology Limited Production of polyketides and other natural products
GB0327720D0 (en) * 2003-11-28 2003-12-31 Biotica Tech Ltd Erythromycins and process for their preparation
GB0327721D0 (en) * 2003-11-28 2003-12-31 Biotica Tech Ltd Polyketides and their synthesis
GB0417852D0 (en) 2004-08-11 2004-09-15 Biotica Tech Ltd Production of polyketides and other natural products
BRPI0909839A2 (pt) * 2008-03-28 2017-06-13 Univ California produção de ácidos dicarboxílicos usando policetídeo sintases
US8420833B2 (en) * 2008-04-29 2013-04-16 The Regents Of The University Of California Producing biofuels using polyketide synthases
GB0904540D0 (en) 2009-03-17 2009-04-29 Biotica Tech Ltd Novel compounds and methods for their production
WO2011011689A2 (en) * 2009-07-23 2011-01-27 The Regents Of The University Of Michigan Method for enzymatic production of decarboxylated polyketides and fatty acids
EP2456777A2 (en) 2009-07-24 2012-05-30 Université Henri Poincaré - Nancy 1 Stambomycin and derivatives, their production and their use as drugs
GB0914589D0 (en) 2009-08-20 2009-09-30 Biotica Tech Ltd Novel compounds and methods for their production
US8852902B2 (en) 2010-11-22 2014-10-07 The Regents Of The University Of California Producing a trimethylpentanoic acid using hybrid polyketide synthases
MX2015014026A (es) 2013-04-04 2016-08-03 Harvard College Macrolidos y metodos para su preparacion y uso.
CN106998685A (zh) 2014-10-08 2017-08-01 哈佛大学的校长及成员们 14‑元酮内酯及其制备和使用方法
WO2016154591A1 (en) 2015-03-25 2016-09-29 President And Fellows Of Harvard College Macrolides with modified desosamine sugars and uses thereof
CN106591388A (zh) * 2016-10-13 2017-04-26 中国农业科学院生物技术研究所 具有聚酮类化合物合成功能的基因Dr2091
WO2018153960A1 (en) 2017-02-22 2018-08-30 Immune System Regulation Holding Ab Novel immune stimulating macrolides
US11059845B2 (en) 2017-02-22 2021-07-13 ISR Immune System Regulation Holding AB (publ) Immune stimulating macrolides
CN110914425B (zh) 2017-06-06 2024-06-25 齐默尔根公司 用于改良刺糖多孢菌的高通量(htp)基因组工程改造平台
WO2019180265A1 (en) 2018-03-23 2019-09-26 Immune System Regulation Holding Ab Combinations of macrolide compounds and immune checkpoint inhibitors

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US5141926A (en) * 1990-04-18 1992-08-25 Abbott Laboratories Erythromycin derivatives
US5712146A (en) * 1993-09-20 1998-01-27 The Leland Stanford Junior University Recombinant combinatorial genetic library for the production of novel polyketides
US5672491A (en) * 1993-09-20 1997-09-30 The Leland Stanford Junior University Recombinant production of novel polyketides
ATE297992T1 (de) * 1993-09-20 2005-07-15 Univ Leland Stanford Junior Recombinante herstellung aromatischer polyketiden
NZ313383A (en) * 1995-07-06 1999-08-30 Univ Leland Stanford Junior Cell-free synthesis of polyketides using pks (polyketide synthase)
PL331285A1 (en) * 1996-07-05 1999-07-05 Biotica Tech Ltd Erythromycins and method of preparing them
JPH10303641A (ja) * 1997-04-23 1998-11-13 Mitsubishi Electric Corp 発振器及び無線機
NZ500693A (en) * 1997-04-30 2004-01-30 Kosan Biosciences Inc Combinatorial polyketide libraries produced using a modular PKS gene cluster as scaffold
AP1060A (en) * 1998-01-02 2002-04-23 Pfizer Prod Inc Novel erythromycin derivatives.

Also Published As

Publication number Publication date
JP4489947B2 (ja) 2010-06-23
TR200003771T2 (tr) 2001-10-22
CA2331764C (en) 2008-03-11
WO2000000618A2 (en) 2000-01-06
WO2000000618A3 (en) 2000-04-27
ID28285A (id) 2001-05-10
PL345575A1 (en) 2001-12-17
KR20010071684A (ko) 2001-07-31
YU82500A (sh) 2004-03-12
KR20010071683A (ko) 2001-07-31
HK1034536A1 (en) 2001-10-26
CA2331764A1 (en) 2000-01-06
ATE321137T1 (de) 2006-04-15
US7198922B2 (en) 2007-04-03
AU763230B2 (en) 2003-07-17
JP2002519032A (ja) 2002-07-02
AU765257B2 (en) 2003-09-11
AU4524799A (en) 2000-01-17
ID27419A (id) 2001-04-05
CA2335523A1 (en) 2000-01-06
PL345578A1 (en) 2001-12-17
MXPA00012885A (es) 2002-07-02
EP1091971B1 (en) 2010-03-17
TR200003770T2 (tr) 2001-04-20
CN1316001A (zh) 2001-10-03
NZ509601A (en) 2004-04-30
CZ20004913A3 (cs) 2001-11-14
US7148045B1 (en) 2006-12-12
WO2000000500A2 (en) 2000-01-06
HUP0105179A2 (hu) 2002-05-29
YU82700A (sh) 2003-10-31
US20050090461A1 (en) 2005-04-28
JP2002519014A (ja) 2002-07-02
AU4524599A (en) 2000-01-17
BR9911710A (pt) 2001-03-20
US20060240528A1 (en) 2006-10-26
CZ20004912A3 (cs) 2001-08-15
MXPA00012936A (es) 2002-06-21
WO2000000500A3 (en) 2000-03-16
EP1091971A2 (en) 2001-04-18
IL140438A0 (en) 2002-02-10
EP1090123B1 (en) 2006-03-22
DE69930510T2 (de) 2006-12-07
DE69930510D1 (de) 2006-05-11
IL140440A0 (en) 2002-02-10
DE69942149D1 (en) 2010-04-29
GB9814006D0 (en) 1998-08-26
HUP0102945A2 (hu) 2001-12-28
ES2341403T3 (es) 2010-06-18
ATE461289T1 (de) 2010-04-15
EP1090123A2 (en) 2001-04-11
NZ509600A (en) 2004-03-26
CN1315956A (zh) 2001-10-03

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