BR9814526A - "processo para o preparo de pró-insulina humana" - Google Patents

"processo para o preparo de pró-insulina humana"

Info

Publication number
BR9814526A
BR9814526A BR9814526-6A BR9814526A BR9814526A BR 9814526 A BR9814526 A BR 9814526A BR 9814526 A BR9814526 A BR 9814526A BR 9814526 A BR9814526 A BR 9814526A
Authority
BR
Brazil
Prior art keywords
insulin
chain
preparation
human
region
Prior art date
Application number
BR9814526-6A
Other languages
English (en)
Inventor
Chung-Il Hong
Jung-Woo Kim
Wang-Sik Lee
Chang-Kyu Kim
Yong In Kim
Je-Nie Pheu
Jeong-Woo Shin
Sung-Jin Oh
Original Assignee
Chong Kun Dang Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chong Kun Dang Corp filed Critical Chong Kun Dang Corp
Publication of BR9814526A publication Critical patent/BR9814526A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Endocrinology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Toxicology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Diabetes (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Patente de Invenção: <B>"PROCESSO PARA O PREPARO DE PRó-INSULINA HUMANA "<D>. A presente invenção refere-se a um processo para o preparo de pró-insulina humana, a qual é representada como a seguinte fórmula química estrutural (I) em que R é um resíduo de aminoácido ou um peptídeo, o qual é degradável enzimática ou quimicamente; e X é uma ligação de um grupo amino de A-1 em uma cadeia A de insulina e um grupo carboxila de B-30 em uma cadeia B de insulina a qual pode ser separada enzimática ou quimicamente da cadeia A ou da cadeia B, contanto que a região de A-1 a A-21 seja a cadeia A de insulina e uma região de B-1 a B-30 seja a cadeia B de insulina. De acordo com a presente invenção, um precursor de insulina humana recombinante pode ser facilmente fabricado com uma boa reprodutibilidade, uma vez que dissolução, sulfonação, concentração, desalinização e puruficação são acentuadamente simplificadas, ao mesmo tempo em que se aumenta o rendimento de reação de reduplicação.
BR9814526-6A 1997-12-29 1998-12-24 "processo para o preparo de pró-insulina humana" BR9814526A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1019970077163A KR100253916B1 (ko) 1997-12-29 1997-12-29 사람 인슐린 전구체의 제조방법
PCT/KR1998/000464 WO1999033988A1 (en) 1997-12-29 1998-12-24 A process for preparing human proinsulin

Publications (1)

Publication Number Publication Date
BR9814526A true BR9814526A (pt) 2000-10-17

Family

ID=19529497

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9814526-6A BR9814526A (pt) 1997-12-29 1998-12-24 "processo para o preparo de pró-insulina humana"

Country Status (10)

Country Link
US (1) US5952461A (pt)
EP (1) EP1042479A1 (pt)
JP (1) JP2002500013A (pt)
KR (1) KR100253916B1 (pt)
CN (1) CN1163600C (pt)
AU (1) AU735480B2 (pt)
BR (1) BR9814526A (pt)
CA (1) CA2315750A1 (pt)
RU (1) RU2203949C2 (pt)
WO (1) WO1999033988A1 (pt)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2686899B1 (fr) * 1992-01-31 1995-09-01 Rhone Poulenc Rorer Sa Nouveaux polypeptides biologiquement actifs, leur preparation et compositions pharmaceutiques les contenant.
WO2001046453A1 (en) * 1999-12-22 2001-06-28 Novo Nordisk A/S Method for extractive refolding of scrambled single-chain polypeptides
EP1314739A1 (en) * 2001-11-22 2003-05-28 Bayer Ag Process for renaturation of recombinant, disulfide containing proteins at high protein concentrations in the presence of amines
ES2500918T3 (es) 2001-12-21 2014-10-01 Human Genome Sciences, Inc. Proteínas de fusión de albúmina e interferón beta
DE10235168A1 (de) * 2002-08-01 2004-02-12 Aventis Pharma Deutschland Gmbh Verfahren zur Reinigung von Preproinsulin
ATE423138T1 (de) * 2002-12-05 2009-03-15 Wockhardt Ltd Verfahren zur extraktion und isolierung von insulin aus recombinanten quellen
US20050026826A1 (en) * 2003-01-17 2005-02-03 Margarethe Hoenig Feline proinsulin, insulin and constituent peptides
DE602004014251D1 (de) * 2003-01-31 2008-07-17 Organon Nv Verfahren zur proteinisolierung unteranoxischen bedingungen
FR2860796B1 (fr) * 2003-10-14 2006-02-10 Centre Nat Rech Scient Procede de dissociation de la molecule d'hemoglobine extracellulaire d'arenicola marina, chaines proteiques constituant ladite molecule et sequences nucleotidiques codant pour lesdites chaines proteiques
CN105801686B (zh) 2004-07-19 2020-04-07 比奥孔有限公司 胰岛素-低聚物共轭物、制剂及其用途
CA2629306A1 (en) 2005-11-23 2007-05-31 Genentech, Inc. Methods and compositions related to b cell assays
CN101173006B (zh) * 2006-10-30 2011-12-21 江苏万邦生化医药股份有限公司 一种重组人胰岛素的制备方法
HUE037633T2 (hu) 2007-07-09 2018-09-28 Genentech Inc Diszulfidkötés redukciójának megelõzése polipeptidek rekombináns elõállítása során
EP2203181B1 (en) 2007-10-16 2018-02-14 Biocon Limited An orally administerable solid pharmaceutical composition and a process thereof
UA91281C2 (ru) * 2008-11-26 2010-07-12 Общество С Ограниченной Ответственностью «Мако» Способ получения рекомбинантного инсулина человека
RU2395296C1 (ru) * 2009-02-19 2010-07-27 Общество С Ограниченной Ответственностью "Концерн О3" Способ получения препарата проинсулина для перорального применения
DK2739646T3 (en) 2011-05-10 2018-02-26 Stefan Hermann COMPREHENSIVE BUFFER SYSTEM FOR RENATURING HUMAN PROINSULIN OR PROINSULIN DERIVATIVES
US10633414B2 (en) 2015-07-27 2020-04-28 Purdue Research Foundation Tandem folding methods to improve protein folding yield
CN105092860A (zh) * 2015-08-25 2015-11-25 江苏省中医药研究院 一种胰岛β细胞内胰岛素原正确折叠率的检测方法
EP3344651B1 (en) 2015-09-02 2022-03-02 Merck Sharp & Dohme Corp. A process for obtaining insulin with correctly formed disulfide bonds
KR20210016400A (ko) 2018-05-24 2021-02-15 지앙수 헨그루이 메디슨 컴퍼니 리미티드 재조합 인간 인슐린 또는 그 유사체의 전구체를 제조하는 방법
WO2020053875A1 (en) * 2018-09-14 2020-03-19 Indian Institute Of Technology, Delhi Process for producing recombinant peptides
KR20200080748A (ko) * 2018-12-27 2020-07-07 주식회사 폴루스 음이온 교환 크로마토그래피를 이용한 인슐린 전구체의 정제방법
CN111690064A (zh) * 2020-06-24 2020-09-22 合肥天麦生物科技发展有限公司 一种胰岛素原前体蛋白的检测方法及其ppi单克隆抗体的制备方法
CN112430252B (zh) * 2020-11-13 2022-09-02 宜昌东阳光长江药业股份有限公司 一种提高目标蛋白回收率的层析方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4430266A (en) * 1980-11-28 1984-02-07 Eli Lilly And Company Process for producing an insulin precursor
NZ199391A (en) * 1981-01-02 1985-12-13 Genentech Inc Chimeric polypeptides comprising a proinsulin sequence,and preparation by recombinant dna technique;production of human insulin
US4451396A (en) * 1983-08-01 1984-05-29 Eli Lilly And Company Process for inhibiting undesired thiol reactions during cyanogen bromide cleavage of peptides
DE3501641A1 (de) * 1985-01-19 1986-07-24 Hoechst Ag, 6230 Frankfurt Verfahren zur gewinnung von insulin-vorlaeufern aus reaktionsgemischen, die bei der faltung von insulin-vorlaeufern aus den entsprechenden s-sulfonaten anfallen
US4616078A (en) * 1985-04-08 1986-10-07 Eli Lilly And Company Process for purifying proinsulin-like materials
US5426036A (en) * 1987-05-05 1995-06-20 Hoechst Aktiengesellschaft Processes for the preparation of foreign proteins in streptomycetes
US4999422A (en) * 1988-04-15 1991-03-12 Biogen, N.V. Continuous method of refolding proteins
DK336188D0 (da) * 1988-06-20 1988-06-20 Nordisk Gentofte Propeptider
DE3901719A1 (de) * 1989-01-21 1990-07-26 Hoechst Ag Verfahren zur herstellung eines insulinvorlaeufers
US5460954A (en) * 1992-04-01 1995-10-24 Cheil Foods & Chemicals, Inc. Production of human proinsulin using a novel vector system
DK0600372T3 (da) * 1992-12-02 1997-08-11 Hoechst Ag Fremgangsmåde til fremstilling af proinsulin med korrekt forbundne cystinbroer.

Also Published As

Publication number Publication date
CN1163600C (zh) 2004-08-25
EP1042479A1 (en) 2000-10-11
RU2203949C2 (ru) 2003-05-10
KR100253916B1 (ko) 2000-05-01
CA2315750A1 (en) 1999-07-08
US5952461A (en) 1999-09-14
KR19990057119A (ko) 1999-07-15
JP2002500013A (ja) 2002-01-08
AU735480B2 (en) 2001-07-12
WO1999033988A1 (en) 1999-07-08
CN1290299A (zh) 2001-04-04
AU1694199A (en) 1999-07-19

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Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE AO NAO RECOLHIMENTO DA(S) 8 A, 9A E 10A ANUIDADE(S).

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO 8.6 DA RPI 1986 DE 27/03/2009.