BR9810192A - Método para proteção das plantas contra insetos e nematódeos - Google Patents
Método para proteção das plantas contra insetos e nematódeosInfo
- Publication number
- BR9810192A BR9810192A BR9810192-7A BR9810192A BR9810192A BR 9810192 A BR9810192 A BR 9810192A BR 9810192 A BR9810192 A BR 9810192A BR 9810192 A BR9810192 A BR 9810192A
- Authority
- BR
- Brazil
- Prior art keywords
- cysteine
- nematodes
- equistatin
- aspartic proteases
- insects
- Prior art date
Links
- 241000238631 Hexapoda Species 0.000 title abstract 4
- 241000244206 Nematoda Species 0.000 title abstract 3
- 102000005927 Cysteine Proteases Human genes 0.000 abstract 5
- 108010005843 Cysteine Proteases Proteins 0.000 abstract 5
- 102000035101 Aspartic proteases Human genes 0.000 abstract 4
- 108091005502 Aspartic proteases Proteins 0.000 abstract 4
- 241000196324 Embryophyta Species 0.000 abstract 3
- 108010062797 equistatin Proteins 0.000 abstract 3
- 241000258916 Leptinotarsa decemlineata Species 0.000 abstract 2
- 102000009843 Thyroglobulin Human genes 0.000 abstract 2
- 108010034949 Thyroglobulin Proteins 0.000 abstract 2
- 241001414989 Thysanoptera Species 0.000 abstract 2
- 241000607479 Yersinia pestis Species 0.000 abstract 2
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 abstract 2
- 235000018417 cysteine Nutrition 0.000 abstract 2
- 108010025752 echistatin Proteins 0.000 abstract 2
- 239000003112 inhibitor Substances 0.000 abstract 2
- 235000018102 proteins Nutrition 0.000 abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 229960002175 thyroglobulin Drugs 0.000 abstract 2
- XLBBKEHLEPNMMF-SSUNCQRMSA-N 129038-42-2 Chemical compound C([C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CS)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(N)=O)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC=1NC=NC=1)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)N1[C@@H](CCC1)C(=O)N[C@@H](C)C(=O)N[C@@H]([C@@H](C)O)C(O)=O)[C@@H](C)O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CS)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CS)NC(=O)[C@H](CS)NC(=O)[C@H]1N(CCC1)C(=O)CNC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CS)NC(=O)[C@@H](N)CCC(O)=O)C1=CC=CC=C1 XLBBKEHLEPNMMF-SSUNCQRMSA-N 0.000 abstract 1
- 241000242760 Actinia equina Species 0.000 abstract 1
- 241000254173 Coleoptera Species 0.000 abstract 1
- 241000255925 Diptera Species 0.000 abstract 1
- 241001517923 Douglasiidae Species 0.000 abstract 1
- 101710097834 Thiol protease Proteins 0.000 abstract 1
- 208000027418 Wounds and injury Diseases 0.000 abstract 1
- 240000008042 Zea mays Species 0.000 abstract 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 abstract 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 abstract 1
- 230000000295 complement effect Effects 0.000 abstract 1
- 235000005822 corn Nutrition 0.000 abstract 1
- 230000006378 damage Effects 0.000 abstract 1
- 102000038379 digestive enzymes Human genes 0.000 abstract 1
- 108091007734 digestive enzymes Proteins 0.000 abstract 1
- 230000035558 fertility Effects 0.000 abstract 1
- 239000012634 fragment Substances 0.000 abstract 1
- 230000002401 inhibitory effect Effects 0.000 abstract 1
- 208000014674 injury Diseases 0.000 abstract 1
- 230000000968 intestinal effect Effects 0.000 abstract 1
- 108010028930 invariant chain Proteins 0.000 abstract 1
- 230000000974 larvacidal effect Effects 0.000 abstract 1
- 150000003573 thiols Chemical class 0.000 abstract 1
- 239000013598 vector Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/50—Isolated enzymes; Isolated proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
- C07K14/8139—Cysteine protease (E.C. 3.4.22) inhibitors, e.g. cystatin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
- C07K14/8142—Aspartate protease (E.C. 3.4.23) inhibitors, e.g. HIV protease inhibitors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8285—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for nematode resistance
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Gastroenterology & Hepatology (AREA)
- Pest Control & Pesticides (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Agronomy & Crop Science (AREA)
- Insects & Arthropods (AREA)
- AIDS & HIV (AREA)
- Virology (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Peptides Or Proteins (AREA)
- Catching Or Destruction (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Patente de Invenção:<B>"MéTODO PARA PROTEçãO DAS PLANTAS CONTRA INSETOS E NEMATóDEOS"<D>. Foi constatado que a equistatina, pertencendo ao domínio tiroglobulina repetido tipo I, e conhecida por ser um inibidor das proteases cisteínas, também inibe proteases aspárticas com um domínio diferente da proteína. O DNA codificando equistatina, um inibidor ou proteases cisteínas e aspárticas, é isolado da anêmona do mar Actinia equina. Foi constatado que a proteína equistatina é particularmente ativa para proteases cisteínas e aspárticas do intestino de uma série de pestes de insetos comuns de colheitas agrícolas, tais como besouro de batata do Colorado, larva da raiz do milho, mosca de leafminer e tripse. Fragmento de cadeia invariante P41, outro membro desta família com somente atividade inibitória da protease cisteína foi igualmente ativo para o complemento da protease cisteína conforme foi encontrado para equistatina. Foi visto que equistatina plena recombinante é larvicida contra besouro de batata do Colorado e reduz fortemente a fecundidade de tripses adultas. DNA codificando equistatina e outras proteínas contendo domínios tiroglobulina repetido tipo I semelhantes foram clonados em vetores e usadas para transformar plantas conferindo deste modo reduzida suscetibilidade a lesão por pestes de plantas, as quais tenham tiol e/ou proteases aspárticas como enzimas digestivas inclusive insetos e nematódeos e particularmente insetos Coleópteros, Dípteros e Tisanópteros.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP97201777 | 1997-06-18 | ||
| PCT/NL1998/000352 WO1998058068A2 (en) | 1997-06-18 | 1998-06-18 | A method for plant protection against insects or nematodes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR9810192A true BR9810192A (pt) | 2000-08-08 |
Family
ID=8228430
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR9810192-7A BR9810192A (pt) | 1997-06-18 | 1998-06-18 | Método para proteção das plantas contra insetos e nematódeos |
Country Status (15)
| Country | Link |
|---|---|
| US (1) | US6861578B1 (pt) |
| EP (1) | EP0991769B1 (pt) |
| JP (1) | JP2002511755A (pt) |
| AT (1) | ATE419365T1 (pt) |
| AU (1) | AU752020B2 (pt) |
| BR (1) | BR9810192A (pt) |
| CA (1) | CA2294421C (pt) |
| CZ (1) | CZ300639B6 (pt) |
| DE (1) | DE69840404D1 (pt) |
| HU (1) | HU225427B1 (pt) |
| NZ (1) | NZ501872A (pt) |
| PL (1) | PL189808B1 (pt) |
| SK (1) | SK172499A3 (pt) |
| TR (1) | TR199903122T2 (pt) |
| WO (1) | WO1998058068A2 (pt) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AP3351A (en) * | 2009-05-22 | 2015-07-31 | Academia Sinica | Extracellular plant ferredoxin-like protein and uses thereof |
| US8424239B1 (en) | 2010-10-12 | 2013-04-23 | Jose A. Gallo | Codling moth trap |
| CN107312786B (zh) * | 2017-07-14 | 2019-10-29 | 内蒙古农业大学 | 一种重组斯氏副柔线虫半胱氨酸蛋白酶的制备方法及应用 |
| CN113403209B (zh) * | 2021-07-30 | 2022-08-26 | 西南大学 | 天冬氨酸蛋白酶基因在改良球孢白僵菌菌株中的应用 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE58909876D1 (de) * | 1988-06-20 | 2000-09-14 | Novartis Ag | Verfahren zur Bekämpfung von Pflanzenschädlingen mit nicht-pflanzlichen Proteinase-Inhibitoren |
| WO1992021753A1 (en) * | 1991-06-07 | 1992-12-10 | Dowelanco | Insecticidal proteins and method for plant protection |
| US5629469A (en) | 1994-03-10 | 1997-05-13 | Sandoz Ltd. | Thiol protease inhibitor |
-
1998
- 1998-06-18 JP JP50420999A patent/JP2002511755A/ja active Pending
- 1998-06-18 DE DE69840404T patent/DE69840404D1/de not_active Expired - Fee Related
- 1998-06-18 AU AU81324/98A patent/AU752020B2/en not_active Ceased
- 1998-06-18 NZ NZ501872A patent/NZ501872A/en unknown
- 1998-06-18 HU HU0001839A patent/HU225427B1/hu not_active IP Right Cessation
- 1998-06-18 PL PL98337826A patent/PL189808B1/pl not_active IP Right Cessation
- 1998-06-18 CZ CZ0450399A patent/CZ300639B6/cs not_active IP Right Cessation
- 1998-06-18 BR BR9810192-7A patent/BR9810192A/pt not_active Application Discontinuation
- 1998-06-18 CA CA002294421A patent/CA2294421C/en not_active Expired - Fee Related
- 1998-06-18 TR TR1999/03122T patent/TR199903122T2/xx unknown
- 1998-06-18 WO PCT/NL1998/000352 patent/WO1998058068A2/en active IP Right Grant
- 1998-06-18 US US09/445,480 patent/US6861578B1/en not_active Expired - Fee Related
- 1998-06-18 EP EP98931124A patent/EP0991769B1/en not_active Expired - Lifetime
- 1998-06-18 SK SK1724-99A patent/SK172499A3/sk unknown
- 1998-06-18 AT AT98931124T patent/ATE419365T1/de not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| EP0991769A2 (en) | 2000-04-12 |
| TR199903122T2 (xx) | 2000-04-21 |
| CZ450399A3 (cs) | 2000-06-14 |
| NZ501872A (en) | 2002-05-31 |
| ATE419365T1 (de) | 2009-01-15 |
| HUP0001839A2 (hu) | 2000-09-28 |
| HU225427B1 (en) | 2006-11-28 |
| AU752020B2 (en) | 2002-09-05 |
| CA2294421A1 (en) | 1998-12-23 |
| CA2294421C (en) | 2009-01-06 |
| US6861578B1 (en) | 2005-03-01 |
| CZ300639B6 (cs) | 2009-07-08 |
| WO1998058068A2 (en) | 1998-12-23 |
| WO1998058068A3 (en) | 1999-03-25 |
| EP0991769B1 (en) | 2008-12-31 |
| JP2002511755A (ja) | 2002-04-16 |
| AU8132498A (en) | 1999-01-04 |
| HUP0001839A3 (en) | 2002-04-29 |
| DE69840404D1 (de) | 2009-02-12 |
| PL189808B1 (pl) | 2005-09-30 |
| PL337826A1 (en) | 2000-09-11 |
| SK172499A3 (en) | 2000-09-12 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B25A | Requested transfer of rights approved |
Free format text: PLANT RESEARCH INTERNATIONAL B.V. (NL) |
|
| B07A | Application suspended after technical examination (opinion) [chapter 7.1 patent gazette] | ||
| B09B | Patent application refused [chapter 9.2 patent gazette] |
Free format text: DE ACORDO COM O ART. 36 DA LPI (LEI 9279/96) 2O, O PEDIDO VOLTA A EXAME SENDO CONSIDERADO NAO PATENTEAVEL PELAS RAZOES EXPOSTAS NO PARECER TECNICO ANTERIOR. PORTANTO, INDEFIRO O PRESENTE PEDIDO DE PATENTE COMO INVENCAO - DE ACORDO COM O ART. 8O DA LPI. |
|
| B09B | Patent application refused [chapter 9.2 patent gazette] |
Free format text: MANTIDO O INDEFERIMENTO UMA VEZ QUE NAO FOI APRESENTADO RECURSO DENTRO DO PRAZO LEGAL. |