BR9714887A - Processo de obtenção de plantas leguminosas (leguminosae) transgênicas contendo dna exógeno - Google Patents

Processo de obtenção de plantas leguminosas (leguminosae) transgênicas contendo dna exógeno

Info

Publication number
BR9714887A
BR9714887A BR9714887-3A BR9714887A BR9714887A BR 9714887 A BR9714887 A BR 9714887A BR 9714887 A BR9714887 A BR 9714887A BR 9714887 A BR9714887 A BR 9714887A
Authority
BR
Brazil
Prior art keywords
leguminous plants
leguminosae
embryonic axis
cells
exogenous dna
Prior art date
Application number
BR9714887-3A
Other languages
English (en)
Inventor
Filho Elibio Leopoldo Rech
Aragao Francisco Jose Lima
Original Assignee
Embrapa Pesquisa Agropecuaria
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Embrapa Pesquisa Agropecuaria filed Critical Embrapa Pesquisa Agropecuaria
Publication of BR9714887A publication Critical patent/BR9714887A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Medicinal Preparation (AREA)

Abstract

Patente de Invenção: <B>"PROCESSO DE OBTENçãO DE PLANTAS LEGUMINOSAS (LEGUMINOSAE) TRANSGêNICAS CONTENDO DNA EXóGENO"<D>. A presente invenção refere-se a um processo para produção de plantas leguminosas transgênicas contendo DNA exógeno compreendendo as etapas de introdução de genes exógenos em células do meristema apical de eixos embrionários de plantas leguminosas através do método de biobalística, indução de multibrotação das células na região meristemátia apical modificada na etapa anterior através do cultivo de seus eixos embrionários em um meio contendo um indutor de multibrotação e seleção das células meristemáticas da região apical transformadas através do cultivo ulterior dos referidos eixos embrionários em um meio contendo um agente de seleção.
BR9714887-3A 1997-10-07 1997-10-07 Processo de obtenção de plantas leguminosas (leguminosae) transgênicas contendo dna exógeno BR9714887A (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/BR1997/000053 WO1999018223A1 (en) 1997-10-07 1997-10-07 A process for obtaining transgenic leguminous plants (leguminosae) containing exogenous dna

Publications (1)

Publication Number Publication Date
BR9714887A true BR9714887A (pt) 2000-08-08

Family

ID=4066043

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9714887-3A BR9714887A (pt) 1997-10-07 1997-10-07 Processo de obtenção de plantas leguminosas (leguminosae) transgênicas contendo dna exógeno

Country Status (14)

Country Link
US (2) US6753458B1 (pt)
EP (1) EP1019516B1 (pt)
CN (1) CN1191364C (pt)
AT (1) ATE268821T1 (pt)
AU (1) AU4545497A (pt)
BR (1) BR9714887A (pt)
CA (1) CA2305576C (pt)
DE (1) DE69729489T2 (pt)
DK (1) DK1019516T3 (pt)
ES (1) ES2223082T3 (pt)
MX (1) MX229419B (pt)
PT (1) PT1019516E (pt)
WO (1) WO1999018223A1 (pt)
ZA (1) ZA992218B (pt)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5914451A (en) * 1998-04-06 1999-06-22 Monsanto Company Efficiency soybean transformation protocol
MXPA00011341A (es) * 1999-03-19 2003-08-06 Embrapa Pesquisa Agropecuaria Proceso para la seleccion de celulas transgenicas en la region meristematica de la planta de algodon, cafe, cacao, platano o uva.
US7098386B1 (en) * 2005-04-20 2006-08-29 The Board Of Trustees Of The University Of Arkansas, Na Cowpea cultivar 92-674
US7098387B1 (en) * 2005-04-20 2006-08-29 The Board Of Trustees Of The University Of Arkansas, Na Cowpea cultivar 95-104
NZ592464A (en) 2008-09-26 2013-05-31 Basf Agrochemical Products Bv Herbicide resistant acetohydroxyacid synthase large subunit (AHAS) mutants and methods of use thereof
UA112969C2 (uk) 2010-08-03 2016-11-25 Сібас Юс Ллс Рослина, стійка до одного або більше ррх-інгібуючих гербіцидів, яка містить мутантний ген протопорфіриноген ix оксидази (ррх)
US20120122223A1 (en) 2010-08-03 2012-05-17 Cibus Us Llc Mutated protoporphyrinogen ix oxidase (ppx) genes
CA2840550A1 (en) 2011-07-22 2013-01-31 Basf Plant Science Company Gmbh Plant transformation method
US9790515B2 (en) 2013-03-14 2017-10-17 Cibus Us Llc Mutated allene oxide synthase 2 (AOS2) genes
US9957515B2 (en) 2013-03-15 2018-05-01 Cibus Us Llc Methods and compositions for targeted gene modification
KR102451570B1 (ko) 2013-03-15 2022-10-06 시버스 유에스 엘엘씨 올리고뉴클레오타이드 매개 유전자 보수를 사용한 표적화된 유전자 변형의 효율을 증가시키기 위한 방법 및 조성물
RS65213B1 (sr) 2014-03-14 2024-03-29 Cibus Us Llc Postupci i kompozicije za povećanje efikasnosti ciljane modifikacije gena korišćenjem reparacije gena posredovane oligonukleotidima
BR112018016278A2 (pt) 2016-02-09 2019-01-02 Cibus Europe Bv métodos e composições para aumentar a eficiência da modificação do gene direcionada usando reparação de gene mediada por oligonucleotídeo
US11359208B2 (en) 2018-01-09 2022-06-14 Cibus Us Llc Shatterproof genes and mutations
CN113106118B (zh) * 2021-05-14 2023-05-12 浙江大学 一种利用草甘膦筛选豇豆遗传转化体的方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4940835A (en) * 1985-10-29 1990-07-10 Monsanto Company Glyphosate-resistant plants
US5378824A (en) * 1986-08-26 1995-01-03 E. I. Du Pont De Nemours And Company Nucleic acid fragment encoding herbicide resistant plant acetolactate synthase
US5015580A (en) 1987-07-29 1991-05-14 Agracetus Particle-mediated transformation of soybean plants and lines
US5565346A (en) * 1988-07-27 1996-10-15 Calgene, Inc. Transformation and regeneration system for legumes
ATE199098T1 (de) 1989-11-17 2001-02-15 Monsanto Co Gegen glutaminsynthetaseinhibitoren resistente sojabohnenpflanzen
US5589583A (en) * 1990-01-11 1996-12-31 Monsanto Company Plant promoter
CA2036935A1 (en) * 1990-02-26 1991-08-27 Paul Christou Plant transformation process with early identification of germ line transformation events
WO1992004449A1 (en) 1990-08-31 1992-03-19 Monsanto Company Glyphosate tolerant 5-enolpyruvylshikimate-3-phosphate synthases
US5633435A (en) * 1990-08-31 1997-05-27 Monsanto Company Glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthases
CA2071767A1 (en) 1991-06-20 1992-12-21 Praveen K. Saxena Hyperproduction of shoots during in vitro regeneration of plant
FR2736929B1 (fr) 1995-07-19 1997-08-22 Rhone Poulenc Agrochimie Sequence adn isolee pouvant servir de zone de regulation dans un gene chimere utilisable pour la transformation des plantes

Also Published As

Publication number Publication date
AU4545497A (en) 1999-04-27
MXPA00003380A (es) 2001-09-01
WO1999018223A1 (en) 1999-04-15
US6753458B1 (en) 2004-06-22
ZA992218B (en) 1999-12-29
DK1019516T3 (da) 2004-09-27
US20040268444A1 (en) 2004-12-30
CA2305576A1 (en) 1999-04-15
ATE268821T1 (de) 2004-06-15
EP1019516B1 (en) 2004-06-09
ES2223082T3 (es) 2005-02-16
DE69729489D1 (de) 2004-07-15
DE69729489T2 (de) 2005-06-30
CN1191364C (zh) 2005-03-02
PT1019516E (pt) 2004-09-30
CN1276015A (zh) 2000-12-06
CA2305576C (en) 2007-05-01
MX229419B (es) 2005-07-25
EP1019516A1 (en) 2000-07-19

Similar Documents

Publication Publication Date Title
BR9714887A (pt) Processo de obtenção de plantas leguminosas (leguminosae) transgênicas contendo dna exógeno
JP7047014B2 (ja) オリゴヌクレオチド仲介型遺伝子修復を使用した標的遺伝子修飾の効率を高めるための方法および組成物
Chawla Introduction to plant biotechnology (3/e)
Bonga Vegetative propagation in relation to juvenility, maturity, and rejuvenation
BR0209745A (pt) Cromossomas artificiais de plantas, usos e dos mesmos e método para preparação de cromossomas artificiais de plantas
Zhou et al. A circulatory system useful both for long-term somatic embryogenesis and genetic transformation in Vitis vinifera L. cv. Thompson Seedless
CN109072248A (zh) 采用寡核苷酸介导的基因修复提高靶向基因修饰的效率的方法和组合物
AR093045A2 (es) Un metodo de aplicar un herbicida a una planta transgenica para producir una semilla hibrida no natural transgenica, celulas no naturales y adn recombinante
BR9507385A (pt) Gene rps2 e usos do mesmo
EP3501268B1 (en) Regeneration of plants in the presence of histone deacetylase inhibitors
RU94046200A (ru) Последовательности днк, производные последовательностей днк, плазмиды, способ применения последовательностей, способ применения плазмид, трансгенные растения
Xie et al. Genetic transformation of grape varieties and rootstocks via organogenesis
US20190390207A1 (en) Method for introducing substance into plant
DK1173594T3 (da) Fremgangsmåder til afgivelse af inhibitorisk RNA til planter og anvendelse deraf
US20220056461A1 (en) Improving plant regeneration
ATE502111T1 (de) Auf replizierende vektoren basierende genexpression in plastiden
Noor et al. Optimization of callus induction and regeneration system for Pakistani wheat cultivars Kohsar and Khyber-87
MXPA02012475A (es) Captura de genes activados ires en plantas.
MXPA02012557A (es) Composiciones y metodos para la modificacion de la expresion del gen.
CN116368230A (zh) 基因组编辑过的植物的生产方法
Andronic et al. In vitro response of anthers from different barley genotypes in order to obtain haploid plants.
Garda Soybean androgenesis
US20070283455A1 (en) Genetic Transformation of Grapevines
DK0587791T3 (da) Forstærkelse af ekspression ved hjælp af gentargeting i endogene retroviruslignende sekvenser
Shekhawat et al. Role of tissue culture techniques in social forestry

Legal Events

Date Code Title Description
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 07/10/1997, OBSERVADAS AS CONDICOES LEGAIS.

B24C Patent annual fee: request for for restoration

Free format text: REFERENTE A(S) 14A ANUIDADE(S).

B24E Patent annual fee: resolution cancelled

Free format text: REFERENTE AO DESPACHO 24.3 PUBLICADO NA RPI 2146 DE 22/02/2012.

B21F Lapse acc. art. 78, item iv - on non-payment of the annual fees in time
B21H Decision of lapse of a patent or of a certificate of addition of invention cancelled [chapter 21.8 patent gazette]
B21A Patent or certificate of addition expired [chapter 21.1 patent gazette]