DK0587791T3 - Forstærkelse af ekspression ved hjælp af gentargeting i endogene retroviruslignende sekvenser - Google Patents

Forstærkelse af ekspression ved hjælp af gentargeting i endogene retroviruslignende sekvenser

Info

Publication number
DK0587791T3
DK0587791T3 DK92913951T DK92913951T DK0587791T3 DK 0587791 T3 DK0587791 T3 DK 0587791T3 DK 92913951 T DK92913951 T DK 92913951T DK 92913951 T DK92913951 T DK 92913951T DK 0587791 T3 DK0587791 T3 DK 0587791T3
Authority
DK
Denmark
Prior art keywords
pct
nucleic acid
sequences
amplification
expression
Prior art date
Application number
DK92913951T
Other languages
English (en)
Inventor
Florian M Wurm
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Application granted granted Critical
Publication of DK0587791T3 publication Critical patent/DK0587791T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
DK92913951T 1991-05-31 1992-05-29 Forstærkelse af ekspression ved hjælp af gentargeting i endogene retroviruslignende sekvenser DK0587791T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US70815591A 1991-05-31 1991-05-31
PCT/US1992/004469 WO1992021763A1 (en) 1991-05-31 1992-05-29 Enhancement of expression by gene targeting in endogenous retrovirus-like sequences

Publications (1)

Publication Number Publication Date
DK0587791T3 true DK0587791T3 (da) 2004-07-26

Family

ID=24844598

Family Applications (1)

Application Number Title Priority Date Filing Date
DK92913951T DK0587791T3 (da) 1991-05-31 1992-05-29 Forstærkelse af ekspression ved hjælp af gentargeting i endogene retroviruslignende sekvenser

Country Status (9)

Country Link
US (1) US5686263A (da)
EP (1) EP0587791B1 (da)
JP (1) JP3449418B2 (da)
AT (1) ATE263837T1 (da)
CA (1) CA2110266C (da)
DE (1) DE69233339T2 (da)
DK (1) DK0587791T3 (da)
ES (1) ES2214474T3 (da)
WO (1) WO1992021763A1 (da)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996037626A1 (en) * 1995-05-22 1996-11-28 Chiron Corporation Position-specific integration of vector constructs into eukaryotic genomes mediated by a chimeric integrase protein
US6509174B2 (en) 1998-06-16 2003-01-21 Ecole Polytechnique Federal De Lausanne (Epfl) Cells, genetically modified with optical marker
US6156514A (en) * 1998-12-03 2000-12-05 Sunol Molecular Corporation Methods for making recombinant cells
AU761136B2 (en) * 1998-12-23 2003-05-29 Genentech Inc. Transfectacons comprising calcium phosphate and a nucleic acid
US20110171729A1 (en) * 2006-05-04 2011-07-14 Ab Maxis Inc. Method for Producing Stable Mammalian Cell Lines Producing High Levels of Recombinant Proteins

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4396601A (en) * 1980-03-26 1983-08-02 The Regents Of The University Of Calif. Gene transfer in intact mammals
PT88556B (pt) * 1987-09-28 1992-11-30 Smithkline Biolog Processo de integracao genomica estavel e de expressao de uma sequencia de cadificacao estranha, em leveduras
EP0445227A4 (en) * 1988-11-23 1991-12-27 The Regents Of The University Of California Position-specific insertion vectors and method of using same
DE69031735T2 (de) * 1989-04-18 1998-03-12 Applied Biotechnology Inc Erzeugung von hybrid-genen und proteinen durch rekombination mittels viren
WO1990014092A1 (en) * 1989-05-18 1990-11-29 Cell Genesys, Inc. Single-strand site-directed modification of mammalian genes in vivo
KR100226158B1 (ko) * 1989-07-25 1999-10-15 스티븐 에이. 서윈. 엠.디. 만능공급세포 및 잡종 포유동물 숙주를 위한 동종 재조합
DK0452484T3 (da) * 1989-11-06 1996-10-14 Cell Genesys Inc Fremstilling af proteiner ved homolog rekombination
WO1992001800A1 (en) * 1990-07-20 1992-02-06 Chiron Corporation Method for integrative transformation of yeast using dispersed repetitive elements
FR2670502B1 (fr) * 1990-12-13 1994-09-16 Eurolysine Cassette d'integration "multisite" dans le genome d'une levure, levure transformee et procede de preparation d'un produit d'interet par une levure ainsi transformee.

Also Published As

Publication number Publication date
CA2110266C (en) 1996-10-08
JP3449418B2 (ja) 2003-09-22
US5686263A (en) 1997-11-11
ATE263837T1 (de) 2004-04-15
WO1992021763A1 (en) 1992-12-10
EP0587791A1 (en) 1994-03-23
CA2110266A1 (en) 1992-12-10
DE69233339D1 (de) 2004-05-13
EP0587791B1 (en) 2004-04-07
ES2214474T3 (es) 2004-09-16
JPH06508745A (ja) 1994-10-06
DE69233339T2 (de) 2005-05-04

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