BR112023003441A2 - Sistemas e métodos para transposição de sequências nucleotídicas de carga - Google Patents
Sistemas e métodos para transposição de sequências nucleotídicas de cargaInfo
- Publication number
- BR112023003441A2 BR112023003441A2 BR112023003441A BR112023003441A BR112023003441A2 BR 112023003441 A2 BR112023003441 A2 BR 112023003441A2 BR 112023003441 A BR112023003441 A BR 112023003441A BR 112023003441 A BR112023003441 A BR 112023003441A BR 112023003441 A2 BR112023003441 A2 BR 112023003441A2
- Authority
- BR
- Brazil
- Prior art keywords
- cargo
- methods
- systems
- nucleic acid
- transposing
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
- Enzymes And Modification Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202063069703P | 2020-08-24 | 2020-08-24 | |
| US202163186698P | 2021-05-10 | 2021-05-10 | |
| US202163232593P | 2021-08-12 | 2021-08-12 | |
| PCT/US2021/047195 WO2022046662A1 (en) | 2020-08-24 | 2021-08-23 | Systems and methods for transposing cargo nucleotide sequences |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112023003441A2 true BR112023003441A2 (pt) | 2023-05-02 |
Family
ID=80353933
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112023003441A BR112023003441A2 (pt) | 2020-08-24 | 2021-08-23 | Sistemas e métodos para transposição de sequências nucleotídicas de carga |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US20240301374A1 (https=) |
| EP (1) | EP4200422A4 (https=) |
| JP (1) | JP2023539237A (https=) |
| KR (1) | KR20230054457A (https=) |
| CN (1) | CN116438302A (https=) |
| AU (1) | AU2021333586A1 (https=) |
| BR (1) | BR112023003441A2 (https=) |
| CA (1) | CA3190758A1 (https=) |
| GB (1) | GB2615658A (https=) |
| MX (1) | MX2023002281A (https=) |
| WO (1) | WO2022046662A1 (https=) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2025508794A (ja) * | 2022-02-23 | 2025-04-10 | メタゲノミ，インク． | 融合タンパク質 |
| US20250327069A1 (en) * | 2022-05-09 | 2025-10-23 | Cornell University | Adaptations for high efficiency and altered pam usage with tn7-crispr-cas transposition systems |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3241902B1 (en) * | 2012-05-25 | 2018-02-28 | The Regents of The University of California | Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription |
| GB201514510D0 (en) * | 2015-08-14 | 2015-09-30 | Nemesis Bioscience Ltd | Delivery vehicle |
| WO2018152244A1 (en) * | 2017-02-14 | 2018-08-23 | Seqwell, Inc. | Compositions and methods for sequencing nucleic acids |
| WO2019090175A1 (en) * | 2017-11-02 | 2019-05-09 | Arbor Biotechnologies, Inc. | Novel crispr-associated transposon systems and components |
| WO2020102709A1 (en) * | 2018-11-16 | 2020-05-22 | The Regents Of The University Of California | Compositions and methods for delivering crispr/cas effector polypeptides |
| CA3124110A1 (en) * | 2018-12-17 | 2020-06-25 | The Broad Institute, Inc. | Crispr-associated transposase systems and methods of use thereof |
| US20240301445A1 (en) * | 2021-01-28 | 2024-09-12 | Arbor Biotechnologies, Inc. | Crispr-associated transposon systems and methods of using same |
-
2021
- 2021-08-23 BR BR112023003441A patent/BR112023003441A2/pt unknown
- 2021-08-23 CN CN202180072470.7A patent/CN116438302A/zh active Pending
- 2021-08-23 AU AU2021333586A patent/AU2021333586A1/en active Pending
- 2021-08-23 JP JP2023513262A patent/JP2023539237A/ja active Pending
- 2021-08-23 WO PCT/US2021/047195 patent/WO2022046662A1/en not_active Ceased
- 2021-08-23 MX MX2023002281A patent/MX2023002281A/es unknown
- 2021-08-23 EP EP21862499.7A patent/EP4200422A4/en active Pending
- 2021-08-23 GB GB2304018.1A patent/GB2615658A/en not_active Withdrawn
- 2021-08-23 KR KR1020237010115A patent/KR20230054457A/ko active Pending
- 2021-08-23 CA CA3190758A patent/CA3190758A1/en active Pending
-
2023
- 2023-10-05 US US18/481,769 patent/US20240301374A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| JP2023539237A (ja) | 2023-09-13 |
| CA3190758A1 (en) | 2022-03-03 |
| GB2615658A (en) | 2023-08-16 |
| MX2023002281A (es) | 2023-05-16 |
| WO2022046662A1 (en) | 2022-03-03 |
| KR20230054457A (ko) | 2023-04-24 |
| CN116438302A (zh) | 2023-07-14 |
| AU2021333586A9 (en) | 2024-02-08 |
| US20240301374A1 (en) | 2024-09-12 |
| AU2021333586A1 (en) | 2023-04-27 |
| EP4200422A1 (en) | 2023-06-28 |
| EP4200422A4 (en) | 2024-08-28 |
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