KR20230054457A - 카고 뉴클레오타이드 서열을 전위시키는 시스템 및 방법 - Google Patents

카고 뉴클레오타이드 서열을 전위시키는 시스템 및 방법 Download PDF

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KR20230054457A
KR20230054457A KR1020237010115A KR20237010115A KR20230054457A KR 20230054457 A KR20230054457 A KR 20230054457A KR 1020237010115 A KR1020237010115 A KR 1020237010115A KR 20237010115 A KR20237010115 A KR 20237010115A KR 20230054457 A KR20230054457 A KR 20230054457A
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sequence
leu
seq
lys
glu
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브라이언 토마스
크리스토퍼 브라운
다니엘라 에스.에이. 골츠만
크리스티나 버터필드
리사 알렉산더
제이슨 리우
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메타지노미, 인크.
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102Mutagenizing nucleic acids
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
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    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
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    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
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    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/10Type of nucleic acid
    • C12N2310/20Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

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  • Wood Science & Technology (AREA)
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  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
KR1020237010115A 2020-08-24 2021-08-23 카고 뉴클레오타이드 서열을 전위시키는 시스템 및 방법 Pending KR20230054457A (ko)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US202063069703P 2020-08-24 2020-08-24
US63/069,703 2020-08-24
US202163186698P 2021-05-10 2021-05-10
US63/186,698 2021-05-10
US202163232593P 2021-08-12 2021-08-12
US63/232,593 2021-08-12
PCT/US2021/047195 WO2022046662A1 (en) 2020-08-24 2021-08-23 Systems and methods for transposing cargo nucleotide sequences

Publications (1)

Publication Number Publication Date
KR20230054457A true KR20230054457A (ko) 2023-04-24

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KR1020237010115A Pending KR20230054457A (ko) 2020-08-24 2021-08-23 카고 뉴클레오타이드 서열을 전위시키는 시스템 및 방법

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Country Link
US (1) US20240301374A1 (https=)
EP (1) EP4200422A4 (https=)
JP (1) JP2023539237A (https=)
KR (1) KR20230054457A (https=)
CN (1) CN116438302A (https=)
AU (1) AU2021333586A1 (https=)
BR (1) BR112023003441A2 (https=)
CA (1) CA3190758A1 (https=)
GB (1) GB2615658A (https=)
MX (1) MX2023002281A (https=)
WO (1) WO2022046662A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2025508794A (ja) * 2022-02-23 2025-04-10 メタゲノミ,インク. 融合タンパク質
US20250327069A1 (en) * 2022-05-09 2025-10-23 Cornell University Adaptations for high efficiency and altered pam usage with tn7-crispr-cas transposition systems

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3241902B1 (en) * 2012-05-25 2018-02-28 The Regents of The University of California Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription
GB201514510D0 (en) * 2015-08-14 2015-09-30 Nemesis Bioscience Ltd Delivery vehicle
WO2018152244A1 (en) * 2017-02-14 2018-08-23 Seqwell, Inc. Compositions and methods for sequencing nucleic acids
WO2019090175A1 (en) * 2017-11-02 2019-05-09 Arbor Biotechnologies, Inc. Novel crispr-associated transposon systems and components
WO2020102709A1 (en) * 2018-11-16 2020-05-22 The Regents Of The University Of California Compositions and methods for delivering crispr/cas effector polypeptides
CA3124110A1 (en) * 2018-12-17 2020-06-25 The Broad Institute, Inc. Crispr-associated transposase systems and methods of use thereof
US20240301445A1 (en) * 2021-01-28 2024-09-12 Arbor Biotechnologies, Inc. Crispr-associated transposon systems and methods of using same

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Publication number Publication date
JP2023539237A (ja) 2023-09-13
CA3190758A1 (en) 2022-03-03
GB2615658A (en) 2023-08-16
MX2023002281A (es) 2023-05-16
WO2022046662A1 (en) 2022-03-03
CN116438302A (zh) 2023-07-14
AU2021333586A9 (en) 2024-02-08
US20240301374A1 (en) 2024-09-12
AU2021333586A1 (en) 2023-04-27
EP4200422A1 (en) 2023-06-28
EP4200422A4 (en) 2024-08-28
BR112023003441A2 (pt) 2023-05-02

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