BR112022008075A2 - Métodos de seqüênciamento de proteínas de célula única e de ácido nucleico - Google Patents
Métodos de seqüênciamento de proteínas de célula única e de ácido nucleicoInfo
- Publication number
- BR112022008075A2 BR112022008075A2 BR112022008075A BR112022008075A BR112022008075A2 BR 112022008075 A2 BR112022008075 A2 BR 112022008075A2 BR 112022008075 A BR112022008075 A BR 112022008075A BR 112022008075 A BR112022008075 A BR 112022008075A BR 112022008075 A2 BR112022008075 A2 BR 112022008075A2
- Authority
- BR
- Brazil
- Prior art keywords
- nucleic acid
- cell
- sequencing methods
- barcode
- acid proteins
- Prior art date
Links
- 238000012163 sequencing technique Methods 0.000 title abstract 4
- 102000039446 nucleic acids Human genes 0.000 title abstract 3
- 108020004707 nucleic acids Proteins 0.000 title abstract 3
- 150000007523 nucleic acids Chemical class 0.000 title abstract 3
- 102000004169 proteins and genes Human genes 0.000 title abstract 3
- 108090000623 proteins and genes Proteins 0.000 title abstract 3
- 230000003321 amplification Effects 0.000 abstract 1
- 239000002207 metabolite Substances 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
- 238000003203 nucleic acid sequencing method Methods 0.000 abstract 1
- 229920001184 polypeptide Polymers 0.000 abstract 1
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6818—Sequencing of polypeptides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/185—Nucleic acid dedicated to use as a hidden marker/bar code, e.g. inclusion of nucleic acids to mark art objects or animals
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Computational Biology (AREA)
- Genetics & Genomics (AREA)
- Food Science & Technology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201962926991P | 2019-10-28 | 2019-10-28 | |
| US202062991425P | 2020-03-18 | 2020-03-18 | |
| PCT/US2020/057656 WO2021086913A1 (en) | 2019-10-28 | 2020-10-28 | Methods of single-cell protein and nucleic acid sequencing |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112022008075A2 true BR112022008075A2 (pt) | 2022-07-12 |
Family
ID=73598182
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112022008075A BR112022008075A2 (pt) | 2019-10-28 | 2020-10-28 | Métodos de seqüênciamento de proteínas de célula única e de ácido nucleico |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US20210139973A1 (https=) |
| EP (1) | EP4041910A1 (https=) |
| JP (1) | JP2023500485A (https=) |
| KR (1) | KR20220108055A (https=) |
| CN (1) | CN114981448A (https=) |
| AU (1) | AU2020374885A1 (https=) |
| BR (1) | BR112022008075A2 (https=) |
| CA (1) | CA3159560A1 (https=) |
| MX (1) | MX2022005093A (https=) |
| WO (1) | WO2021086913A1 (https=) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201715684D0 (en) * | 2017-09-28 | 2017-11-15 | Univ Gent | Means and methods for single molecule peptide sequencing |
| AU2019380606B2 (en) | 2018-11-15 | 2026-04-23 | Quantum-Si Incorporated | Methods and compositions for protein sequencing |
| EP4041910A1 (en) * | 2019-10-28 | 2022-08-17 | Quantum-Si Incorporated | Methods of single-cell protein and nucleic acid sequencing |
| EP4143579A2 (en) | 2020-05-20 | 2023-03-08 | Quantum-si Incorporated | Methods and compositions for protein sequencing |
| KR20240099038A (ko) | 2022-12-21 | 2024-06-28 | 재단법인대구경북과학기술원 | 단일세포 프로테오믹스 분석용 조성물, 이를 이용한 시료 처리방법 및 초소량 펩타이드 표지 일원화 방법 |
| EP4669770A1 (en) * | 2023-02-24 | 2025-12-31 | The Board of Trustees of the Leland Stanford Junior University | SEQUENCE PROCESSES FOR POLYPEPTIDES AND ASSOCIATED COMPONENTS |
| US20250147035A1 (en) * | 2023-11-06 | 2025-05-08 | Quantum-Si Incorporated | Methods for detection of post-translational modifications |
| KR20250093178A (ko) | 2023-12-15 | 2025-06-24 | 재단법인대구경북과학기술원 | 초소량 생체 시료내 바이오 마커 단백질 검출하기 위한 초소량 생체시료 전처리 키트 및 이를 이용한 생체 시료 전처리 방법 |
| WO2026024768A1 (en) * | 2024-07-22 | 2026-01-29 | Quantum-Si Incorporated | Solid phase library preparation for polypeptide sequencing |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2496294A1 (en) | 2005-02-07 | 2006-08-07 | The University Of British Columbia | Apparatus and methods for concentrating and separating particles such as molecules |
| CA2745197A1 (en) * | 2008-12-01 | 2010-06-10 | Research Triangle Institute | Concurrent identification of multitudes of polypeptides |
| NL2009191C2 (en) * | 2012-07-16 | 2014-01-20 | Univ Delft Tech | Single molecule protein sequencing. |
| US9435810B2 (en) * | 2013-03-15 | 2016-09-06 | Washington University | Molecules and methods for iterative polypeptide analysis and processing |
| CA3208970A1 (en) * | 2014-09-15 | 2016-05-06 | Board Of Regents, The University Of Texas System | Improved single molecule peptide sequencing |
| EP3452591B1 (en) * | 2016-05-02 | 2023-08-16 | Encodia, Inc. | Macromolecule analysis employing nucleic acid encoding |
| CN118853848A (zh) * | 2016-08-31 | 2024-10-29 | 哈佛学院董事及会员团体 | 将生物分子的检测组合到使用荧光原位测序的单个试验的方法 |
| US11072816B2 (en) * | 2017-05-03 | 2021-07-27 | The Broad Institute, Inc. | Single-cell proteomic assay using aptamers |
| CA3081446A1 (en) * | 2017-10-31 | 2019-05-09 | Encodia, Inc. | Methods and compositions for polypeptide analysis |
| US11841371B2 (en) * | 2018-03-13 | 2023-12-12 | The Broad Institute, Inc. | Proteomics and spatial patterning using antenna networks |
| CA3117476A1 (en) * | 2018-10-05 | 2020-04-09 | Board Of Regents, The University Of Texas System | Solid-phase n-terminal peptide capture and release |
| EP3941631A4 (en) * | 2019-03-22 | 2022-12-14 | Augmenta Bioworks, Inc. | ISOLATION OF SINGLE CELLS AND THEIR USES |
| US20220221467A1 (en) * | 2019-05-31 | 2022-07-14 | President And Fellows Of Harvard College | Systems and methods for ms1-based mass identification including super-resolution techniques |
| CA3149852A1 (en) * | 2019-09-13 | 2021-03-18 | Lauren Schiff | Methods and compositions for protein and peptide sequencing |
| EP4041910A1 (en) * | 2019-10-28 | 2022-08-17 | Quantum-Si Incorporated | Methods of single-cell protein and nucleic acid sequencing |
-
2020
- 2020-10-28 EP EP20812464.4A patent/EP4041910A1/en not_active Withdrawn
- 2020-10-28 AU AU2020374885A patent/AU2020374885A1/en active Pending
- 2020-10-28 BR BR112022008075A patent/BR112022008075A2/pt not_active Application Discontinuation
- 2020-10-28 WO PCT/US2020/057656 patent/WO2021086913A1/en not_active Ceased
- 2020-10-28 JP JP2022525104A patent/JP2023500485A/ja active Pending
- 2020-10-28 MX MX2022005093A patent/MX2022005093A/es unknown
- 2020-10-28 US US17/082,918 patent/US20210139973A1/en not_active Abandoned
- 2020-10-28 CA CA3159560A patent/CA3159560A1/en active Pending
- 2020-10-28 CN CN202080091891.XA patent/CN114981448A/zh active Pending
- 2020-10-28 KR KR1020227017591A patent/KR20220108055A/ko not_active Ceased
-
2024
- 2024-03-07 US US18/599,113 patent/US20240344122A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| AU2020374885A1 (en) | 2022-06-02 |
| JP2023500485A (ja) | 2023-01-06 |
| MX2022005093A (es) | 2022-08-11 |
| EP4041910A1 (en) | 2022-08-17 |
| CN114981448A (zh) | 2022-08-30 |
| US20240344122A1 (en) | 2024-10-17 |
| CA3159560A1 (en) | 2021-05-06 |
| WO2021086913A1 (en) | 2021-05-06 |
| KR20220108055A (ko) | 2022-08-02 |
| US20210139973A1 (en) | 2021-05-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| BR112022008075A2 (pt) | Métodos de seqüênciamento de proteínas de célula única e de ácido nucleico | |
| BR112021025130A2 (pt) | Reagentes e métodos para replicação, transcrição e tradução em organismos semissintéticos | |
| MX2020004578A (es) | Composiciones de casz y metodos de uso. | |
| CL2021002740A1 (es) | Una proteína crispr de prevotella y francisella 1 (cpf1) aislada de acidaminococcus sp. bv3l6 (ascpf1); proteína de fusión que la comprende; ácido nucleico; vector; métodos para alterar el genoma de una célula o una molécula de adnbc in vitro que utilizan dichas proteínas (divisional de sol. 201903004) | |
| PE20181923A1 (es) | Neoantigenos y metodos de su uso | |
| BR112016015512A2 (pt) | proteínas quiméricas de fator viii e suas utilizações | |
| BR112022027096A2 (pt) | Composições e métodos relacionados a agentes terapêuticos ativáveis | |
| BR112014023869A2 (pt) | proteínas nutritivas carregadas e métodos | |
| MX2021015347A (es) | Técnicas para la identificación de proteínas utilizando el aprendizaje automático y sistemas y métodos afines. | |
| BR112022010153A2 (pt) | Peptídeos, ácido nucleico, polipeptídeo, composição, complexo bioluminescente, métodos, dipeptídeo, sistema e kit | |
| IL277264B2 (en) | Microchip capillary electrophoresis assays and reagents | |
| SA521421271B1 (ar) | تركيبة وطريقة لثبات الأحماض النووية في عينات بيولوجية | |
| AR095596A1 (es) | Moléculas de unión de cadena única comprendiendo n-terminal abp | |
| MX2017008817A (es) | Composiciones y metodos para la glicosilacion de proteinas. | |
| BR112017017025A2 (pt) | métodos e composições para analizar componentes celulares | |
| BR112019005144A2 (pt) | sistema regulador de expressão, composição, métodos para expressar uma proteína, para induzir/reativar a proliferação de cardiomiócitos neonatais ou adultos e para expressar um gene de interesse, vetor, kit regulador de expressão, e, uso. | |
| PL1966240T6 (pl) | Peptydy laktoferyny użyteczne jako peptydy penetrujące komórkę | |
| BR112023001045A2 (pt) | Métodos para clivar um anticorpo e analisar a sequência de um anticorpo, composição e kit para digestão de uma proteína | |
| BR112018074853A2 (pt) | métodos para detectar a presença de pelo menos um genogrupo de norovírus em uma amostra de fezes e para selecionar um paciente que sofre de gastroenterite aguda, e, kit | |
| MY208607A (en) | Cyclooctatetraene containing dyes and compositions | |
| BR112023025331A2 (pt) | Proteína multiespecífica, composição farmacêutica, célula recombinante, ácido nucleico ou conjunto de ácidos nucleicos, uso de uma proteína ou composição, métodos ou uso | |
| BR112017012484A2 (pt) | vírus isolado, cultura celular, gene que codifica uma proteína, proteína, fragmento de dna, vírus de vetor recombinante vivo, pseudo-partícula, vacina, anticorpo ou antissoro, método para a preparação de uma vacina, e, kit de teste diagnóstico. | |
| AR118536A1 (es) | Composiciones y métodos para estabilizar formulaciones que contienen proteína | |
| BR112019010034A2 (pt) | fator viii alvejado de hemácia e método de uso do mesmo | |
| BR112022006866A2 (pt) | Métodos para caracterizar proteínas de células hospedeiras |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B11A | Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing | ||
| B11Y | Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette] |