CA3159560A1 - Methods of single-cell protein and nucleic acid sequencing - Google Patents

Methods of single-cell protein and nucleic acid sequencing

Info

Publication number
CA3159560A1
CA3159560A1 CA3159560A CA3159560A CA3159560A1 CA 3159560 A1 CA3159560 A1 CA 3159560A1 CA 3159560 A CA3159560 A CA 3159560A CA 3159560 A CA3159560 A CA 3159560A CA 3159560 A1 CA3159560 A1 CA 3159560A1
Authority
CA
Canada
Prior art keywords
barcoded
sample
amino acid
molecules
polypeptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3159560A
Other languages
English (en)
French (fr)
Inventor
Matthew Dyer
Brian Reed
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Quantum Si Inc
Original Assignee
Quantum Si Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Quantum Si Inc filed Critical Quantum Si Inc
Publication of CA3159560A1 publication Critical patent/CA3159560A1/en
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6818—Sequencing of polypeptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/185—Nucleic acid dedicated to use as a hidden marker/bar code, e.g. inclusion of nucleic acids to mark art objects or animals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Genetics & Genomics (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA3159560A 2019-10-28 2020-10-28 Methods of single-cell protein and nucleic acid sequencing Pending CA3159560A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201962926991P 2019-10-28 2019-10-28
US62/926,991 2019-10-28
US202062991425P 2020-03-18 2020-03-18
US62/991,425 2020-03-18
PCT/US2020/057656 WO2021086913A1 (en) 2019-10-28 2020-10-28 Methods of single-cell protein and nucleic acid sequencing

Publications (1)

Publication Number Publication Date
CA3159560A1 true CA3159560A1 (en) 2021-05-06

Family

ID=73598182

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3159560A Pending CA3159560A1 (en) 2019-10-28 2020-10-28 Methods of single-cell protein and nucleic acid sequencing

Country Status (10)

Country Link
US (2) US20210139973A1 (https=)
EP (1) EP4041910A1 (https=)
JP (1) JP2023500485A (https=)
KR (1) KR20220108055A (https=)
CN (1) CN114981448A (https=)
AU (1) AU2020374885A1 (https=)
BR (1) BR112022008075A2 (https=)
CA (1) CA3159560A1 (https=)
MX (1) MX2022005093A (https=)
WO (1) WO2021086913A1 (https=)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201715684D0 (en) * 2017-09-28 2017-11-15 Univ Gent Means and methods for single molecule peptide sequencing
AU2019380606B2 (en) 2018-11-15 2026-04-23 Quantum-Si Incorporated Methods and compositions for protein sequencing
EP4041910A1 (en) * 2019-10-28 2022-08-17 Quantum-Si Incorporated Methods of single-cell protein and nucleic acid sequencing
EP4143579A2 (en) 2020-05-20 2023-03-08 Quantum-si Incorporated Methods and compositions for protein sequencing
KR20240099038A (ko) 2022-12-21 2024-06-28 재단법인대구경북과학기술원 단일세포 프로테오믹스 분석용 조성물, 이를 이용한 시료 처리방법 및 초소량 펩타이드 표지 일원화 방법
EP4669770A1 (en) * 2023-02-24 2025-12-31 The Board of Trustees of the Leland Stanford Junior University SEQUENCE PROCESSES FOR POLYPEPTIDES AND ASSOCIATED COMPONENTS
US20250147035A1 (en) * 2023-11-06 2025-05-08 Quantum-Si Incorporated Methods for detection of post-translational modifications
KR20250093178A (ko) 2023-12-15 2025-06-24 재단법인대구경북과학기술원 초소량 생체 시료내 바이오 마커 단백질 검출하기 위한 초소량 생체시료 전처리 키트 및 이를 이용한 생체 시료 전처리 방법
WO2026024768A1 (en) * 2024-07-22 2026-01-29 Quantum-Si Incorporated Solid phase library preparation for polypeptide sequencing

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2496294A1 (en) 2005-02-07 2006-08-07 The University Of British Columbia Apparatus and methods for concentrating and separating particles such as molecules
CA2745197A1 (en) * 2008-12-01 2010-06-10 Research Triangle Institute Concurrent identification of multitudes of polypeptides
NL2009191C2 (en) * 2012-07-16 2014-01-20 Univ Delft Tech Single molecule protein sequencing.
US9435810B2 (en) * 2013-03-15 2016-09-06 Washington University Molecules and methods for iterative polypeptide analysis and processing
CA3208970A1 (en) * 2014-09-15 2016-05-06 Board Of Regents, The University Of Texas System Improved single molecule peptide sequencing
EP3452591B1 (en) * 2016-05-02 2023-08-16 Encodia, Inc. Macromolecule analysis employing nucleic acid encoding
CN118853848A (zh) * 2016-08-31 2024-10-29 哈佛学院董事及会员团体 将生物分子的检测组合到使用荧光原位测序的单个试验的方法
US11072816B2 (en) * 2017-05-03 2021-07-27 The Broad Institute, Inc. Single-cell proteomic assay using aptamers
CA3081446A1 (en) * 2017-10-31 2019-05-09 Encodia, Inc. Methods and compositions for polypeptide analysis
US11841371B2 (en) * 2018-03-13 2023-12-12 The Broad Institute, Inc. Proteomics and spatial patterning using antenna networks
CA3117476A1 (en) * 2018-10-05 2020-04-09 Board Of Regents, The University Of Texas System Solid-phase n-terminal peptide capture and release
EP3941631A4 (en) * 2019-03-22 2022-12-14 Augmenta Bioworks, Inc. ISOLATION OF SINGLE CELLS AND THEIR USES
US20220221467A1 (en) * 2019-05-31 2022-07-14 President And Fellows Of Harvard College Systems and methods for ms1-based mass identification including super-resolution techniques
CA3149852A1 (en) * 2019-09-13 2021-03-18 Lauren Schiff Methods and compositions for protein and peptide sequencing
EP4041910A1 (en) * 2019-10-28 2022-08-17 Quantum-Si Incorporated Methods of single-cell protein and nucleic acid sequencing

Also Published As

Publication number Publication date
AU2020374885A1 (en) 2022-06-02
JP2023500485A (ja) 2023-01-06
BR112022008075A2 (pt) 2022-07-12
MX2022005093A (es) 2022-08-11
EP4041910A1 (en) 2022-08-17
CN114981448A (zh) 2022-08-30
US20240344122A1 (en) 2024-10-17
WO2021086913A1 (en) 2021-05-06
KR20220108055A (ko) 2022-08-02
US20210139973A1 (en) 2021-05-13

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