CN114981448A - 单细胞蛋白质和核酸测序的方法 - Google Patents

单细胞蛋白质和核酸测序的方法 Download PDF

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Publication number
CN114981448A
CN114981448A CN202080091891.XA CN202080091891A CN114981448A CN 114981448 A CN114981448 A CN 114981448A CN 202080091891 A CN202080091891 A CN 202080091891A CN 114981448 A CN114981448 A CN 114981448A
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barcoded
sample
amino acid
polypeptide
molecules
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CN202080091891.XA
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Chinese (zh)
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马修·戴尔
布莱恩·瑞德
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Quantum Si Inc
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Quantum Si Inc
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6818—Sequencing of polypeptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/185—Nucleic acid dedicated to use as a hidden marker/bar code, e.g. inclusion of nucleic acids to mark art objects or animals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Genetics & Genomics (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN202080091891.XA 2019-10-28 2020-10-28 单细胞蛋白质和核酸测序的方法 Pending CN114981448A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201962926991P 2019-10-28 2019-10-28
US62/926,991 2019-10-28
US202062991425P 2020-03-18 2020-03-18
US62/991,425 2020-03-18
PCT/US2020/057656 WO2021086913A1 (en) 2019-10-28 2020-10-28 Methods of single-cell protein and nucleic acid sequencing

Publications (1)

Publication Number Publication Date
CN114981448A true CN114981448A (zh) 2022-08-30

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CN202080091891.XA Pending CN114981448A (zh) 2019-10-28 2020-10-28 单细胞蛋白质和核酸测序的方法

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US (2) US20210139973A1 (https=)
EP (1) EP4041910A1 (https=)
JP (1) JP2023500485A (https=)
KR (1) KR20220108055A (https=)
CN (1) CN114981448A (https=)
AU (1) AU2020374885A1 (https=)
BR (1) BR112022008075A2 (https=)
CA (1) CA3159560A1 (https=)
MX (1) MX2022005093A (https=)
WO (1) WO2021086913A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI915946B (zh) 2023-09-08 2026-02-21 大陸商北京貝瑞和康生物技術有限公司 動態突變str和vntr基因位點的檢測探針組、試劑盒及方法

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201715684D0 (en) * 2017-09-28 2017-11-15 Univ Gent Means and methods for single molecule peptide sequencing
AU2019380606B2 (en) 2018-11-15 2026-04-23 Quantum-Si Incorporated Methods and compositions for protein sequencing
EP4041910A1 (en) * 2019-10-28 2022-08-17 Quantum-Si Incorporated Methods of single-cell protein and nucleic acid sequencing
EP4143579A2 (en) 2020-05-20 2023-03-08 Quantum-si Incorporated Methods and compositions for protein sequencing
KR20240099038A (ko) 2022-12-21 2024-06-28 재단법인대구경북과학기술원 단일세포 프로테오믹스 분석용 조성물, 이를 이용한 시료 처리방법 및 초소량 펩타이드 표지 일원화 방법
EP4669770A1 (en) * 2023-02-24 2025-12-31 The Board of Trustees of the Leland Stanford Junior University SEQUENCE PROCESSES FOR POLYPEPTIDES AND ASSOCIATED COMPONENTS
US20250147035A1 (en) * 2023-11-06 2025-05-08 Quantum-Si Incorporated Methods for detection of post-translational modifications
KR20250093178A (ko) 2023-12-15 2025-06-24 재단법인대구경북과학기술원 초소량 생체 시료내 바이오 마커 단백질 검출하기 위한 초소량 생체시료 전처리 키트 및 이를 이용한 생체 시료 전처리 방법
WO2026024768A1 (en) * 2024-07-22 2026-01-29 Quantum-Si Incorporated Solid phase library preparation for polypeptide sequencing

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20180320224A1 (en) * 2017-05-03 2018-11-08 The Broad Institute, Inc. Single-cell proteomic assay using aptamers
CN109923216A (zh) * 2016-08-31 2019-06-21 哈佛学院董事及会员团体 将生物分子的检测组合到使用荧光原位测序的单个试验的方法
CN110199019A (zh) * 2016-05-02 2019-09-03 Encodia有限公司 采用核酸编码的大分子分析

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CA2496294A1 (en) 2005-02-07 2006-08-07 The University Of British Columbia Apparatus and methods for concentrating and separating particles such as molecules
CA2745197A1 (en) * 2008-12-01 2010-06-10 Research Triangle Institute Concurrent identification of multitudes of polypeptides
NL2009191C2 (en) * 2012-07-16 2014-01-20 Univ Delft Tech Single molecule protein sequencing.
US9435810B2 (en) * 2013-03-15 2016-09-06 Washington University Molecules and methods for iterative polypeptide analysis and processing
CA3208970A1 (en) * 2014-09-15 2016-05-06 Board Of Regents, The University Of Texas System Improved single molecule peptide sequencing
CA3081446A1 (en) * 2017-10-31 2019-05-09 Encodia, Inc. Methods and compositions for polypeptide analysis
US11841371B2 (en) * 2018-03-13 2023-12-12 The Broad Institute, Inc. Proteomics and spatial patterning using antenna networks
CA3117476A1 (en) * 2018-10-05 2020-04-09 Board Of Regents, The University Of Texas System Solid-phase n-terminal peptide capture and release
EP3941631A4 (en) * 2019-03-22 2022-12-14 Augmenta Bioworks, Inc. ISOLATION OF SINGLE CELLS AND THEIR USES
US20220221467A1 (en) * 2019-05-31 2022-07-14 President And Fellows Of Harvard College Systems and methods for ms1-based mass identification including super-resolution techniques
CA3149852A1 (en) * 2019-09-13 2021-03-18 Lauren Schiff Methods and compositions for protein and peptide sequencing
EP4041910A1 (en) * 2019-10-28 2022-08-17 Quantum-Si Incorporated Methods of single-cell protein and nucleic acid sequencing

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110199019A (zh) * 2016-05-02 2019-09-03 Encodia有限公司 采用核酸编码的大分子分析
CN109923216A (zh) * 2016-08-31 2019-06-21 哈佛学院董事及会员团体 将生物分子的检测组合到使用荧光原位测序的单个试验的方法
US20180320224A1 (en) * 2017-05-03 2018-11-08 The Broad Institute, Inc. Single-cell proteomic assay using aptamers

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI915946B (zh) 2023-09-08 2026-02-21 大陸商北京貝瑞和康生物技術有限公司 動態突變str和vntr基因位點的檢測探針組、試劑盒及方法

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AU2020374885A1 (en) 2022-06-02
JP2023500485A (ja) 2023-01-06
BR112022008075A2 (pt) 2022-07-12
MX2022005093A (es) 2022-08-11
EP4041910A1 (en) 2022-08-17
US20240344122A1 (en) 2024-10-17
CA3159560A1 (en) 2021-05-06
WO2021086913A1 (en) 2021-05-06
KR20220108055A (ko) 2022-08-02
US20210139973A1 (en) 2021-05-13

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