BR112022007945A2 - Método para enumeração de bactérias em amostras líquidas, e portador de amostra usado para este método - Google Patents

Método para enumeração de bactérias em amostras líquidas, e portador de amostra usado para este método

Info

Publication number
BR112022007945A2
BR112022007945A2 BR112022007945A BR112022007945A BR112022007945A2 BR 112022007945 A2 BR112022007945 A2 BR 112022007945A2 BR 112022007945 A BR112022007945 A BR 112022007945A BR 112022007945 A BR112022007945 A BR 112022007945A BR 112022007945 A2 BR112022007945 A2 BR 112022007945A2
Authority
BR
Brazil
Prior art keywords
sample
liquid sample
sample carrier
enumeration
bacteria
Prior art date
Application number
BR112022007945A
Other languages
English (en)
Inventor
Poglajen Rok
Zupin Gregor
Ott Rutar Sabina
Original Assignee
Microbium D O O
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Microbium D O O filed Critical Microbium D O O
Publication of BR112022007945A2 publication Critical patent/BR112022007945A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/06—Quantitative determination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/34—Internal compartments or partitions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/10—Enterobacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/21—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Pseudomonadaceae (F)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/24—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • G01N2333/245—Escherichia (G)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/32—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Bacillus (G)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Clinical Laboratory Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

MÉTODO PARA ENUMERAÇÃO DE BACTÉRIAS EM AMOSTRAS LÍQUIDAS, E PORTADOR DE AMOSTRA USADO PARA ESTE MÉTODO. A presente invenção se refere a um método para detecção e/ou quantificação de micro-organismo em uma amostra líquida, em particular em uma amostra de água, o método compreendendo as etapas de: (a) distribuir a amostra líquida em inúmeras diferentes partes de volume discretas em um padrão de distribuição linear, ou diluir a amostra líquida em inúmeras amostras de diluição por um fator de diluição de um padrão de distribuição linear; (b) permitir que o micro-organismo cresça; e (c) aplicar o método do Número Mais Provável nas partes de volume linearmente distribuídas ou nas amostras de diluição linearmente diluídas para detectar e/ou quantificar o micro-organismo. A invenção também revela um portador de amostra para detecção e/ou quantificação de micro-organismo em uma amostra líquida, em que o portador de amostra é estruturado para portar a amostra líquida em inúmeros compartimentos diferentes, em que os compartimentos diferentes, respectivamente, definem uma distribuição de volume linear.
BR112022007945A 2019-11-04 2020-11-03 Método para enumeração de bactérias em amostras líquidas, e portador de amostra usado para este método BR112022007945A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SI201900207A SI25919A (sl) 2019-11-04 2019-11-04 Metoda za določitev najverjetnejšega števila bakterij v tekočih vzorcih in model za porazdelitev vzorcev, uporaben za to metodo
PCT/EP2020/080746 WO2021089504A1 (en) 2019-11-04 2020-11-03 Method for enumeration of bacteria in liquid samples, and sample holder useful for this method

Publications (1)

Publication Number Publication Date
BR112022007945A2 true BR112022007945A2 (pt) 2022-07-12

Family

ID=73172651

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112022007945A BR112022007945A2 (pt) 2019-11-04 2020-11-03 Método para enumeração de bactérias em amostras líquidas, e portador de amostra usado para este método

Country Status (6)

Country Link
US (1) US20220411845A1 (pt)
EP (1) EP4110937A1 (pt)
CN (1) CN114641579A (pt)
BR (1) BR112022007945A2 (pt)
SI (1) SI25919A (pt)
WO (1) WO2021089504A1 (pt)

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2106539B (en) 1981-09-12 1985-11-20 Simon Peter Halfacree Bacteriological mpn strip
US6365368B1 (en) 1985-05-10 2002-04-02 Igen International, Inc. Rapid method for the detection and quantification of microbes in water
US4868110A (en) 1986-10-06 1989-09-19 Biocontrol Systems, Inc. Methods and device for detection of microorganisms
US5518892A (en) 1994-02-23 1996-05-21 Idexx Laboratories, Inc. Apparatus and method for quantification of biological material in a liquid sample
US5985594A (en) 1995-11-14 1999-11-16 Idexx Laboratories, Inc. Method for quantification of biological material in a sample
US6696286B1 (en) 1997-04-09 2004-02-24 3M Innovative Properties Company Method and devices for detecting and enumerating microorganisms
EP1042499A2 (en) 1997-10-27 2000-10-11 Idexx Laboratories, Inc. Device and methods for determination of analyte in a solution
US6492133B1 (en) 2000-05-01 2002-12-10 3M Innovative Properties Company Reflective disc assay devices, systems and methods
US7582472B2 (en) 2003-08-26 2009-09-01 Smith Kenneth E Apparatus and method for liquid sample testing
US9012209B2 (en) 2008-01-17 2015-04-21 Neogen Corporation CO2 optical sensor for detection and enumeration of microorganisms
EP2350308A4 (en) 2008-10-20 2012-07-11 Photonic Biosystems Inc BIOLOGICAL ASSAY USING A NETWORK OF MULTIPLE FILTERS
CN202807433U (zh) 2012-01-20 2013-03-20 安泰凯生物技术有限公司 样品盛装装置
WO2016028834A1 (en) 2014-08-20 2016-02-25 3M Innovative Properties Company Devices and methods for sample partitioning and analysis
GB201417271D0 (en) 2014-09-30 2014-11-12 Brightwater Diagnostics Ltd Testing devices

Also Published As

Publication number Publication date
CN114641579A (zh) 2022-06-17
US20220411845A1 (en) 2022-12-29
WO2021089504A1 (en) 2021-05-14
EP4110937A1 (en) 2023-01-04
SI25919A (sl) 2021-05-31

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