BR112022003089A2 - Métodos de produção de células progenitoras hemogênicas a partir de células-tronco pluripotentes - Google Patents

Métodos de produção de células progenitoras hemogênicas a partir de células-tronco pluripotentes

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Publication number
BR112022003089A2
BR112022003089A2 BR112022003089A BR112022003089A BR112022003089A2 BR 112022003089 A2 BR112022003089 A2 BR 112022003089A2 BR 112022003089 A BR112022003089 A BR 112022003089A BR 112022003089 A BR112022003089 A BR 112022003089A BR 112022003089 A2 BR112022003089 A2 BR 112022003089A2
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BR
Brazil
Prior art keywords
cells
hemogenic
methods
pluripotent stem
producing
Prior art date
Application number
BR112022003089A
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English (en)
Portuguese (pt)
Inventor
Tao Yang Cheng
Lee Carpenter
Original Assignee
Adaptimmune Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Adaptimmune Ltd filed Critical Adaptimmune Ltd
Publication of BR112022003089A2 publication Critical patent/BR112022003089A2/pt

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/10—Cellular immunotherapy characterised by the cell type used
    • A61K40/11—T-cells, e.g. tumour infiltrating lymphocytes [TIL] or regulatory T [Treg] cells; Lymphokine-activated killer [LAK] cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/30—Cellular immunotherapy characterised by the recombinant expression of specific molecules in the cells of the immune system
    • A61K40/32—T-cell receptors [TCR]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/40—Cellular immunotherapy characterised by antigens that are targeted or presented by cells of the immune system
    • A61K40/41—Vertebrate antigens
    • A61K40/42—Cancer antigens
    • A—HUMAN NECESSITIES
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/40—Cellular immunotherapy characterised by antigens that are targeted or presented by cells of the immune system
    • A61K40/41—Vertebrate antigens
    • A61K40/42—Cancer antigens
    • A61K40/428—Undefined tumor antigens, e.g. tumor lysate or antigens targeted by cells isolated from tumor
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
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    • C12N5/0636—T lymphocytes
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2121/00—Preparations for use in therapy
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2300/00—Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/20—Cytokines; Chemokines
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    • C12N2501/20—Cytokines; Chemokines
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
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BR112022003089A 2019-08-20 2020-08-20 Métodos de produção de células progenitoras hemogênicas a partir de células-tronco pluripotentes BR112022003089A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1911957.7A GB201911957D0 (en) 2019-08-20 2019-08-20 Methods of producing haemogenic progenitor cells from pluripotent stem cells
PCT/EP2020/073396 WO2021032851A1 (en) 2019-08-20 2020-08-20 Methods of producing haemogenic progenitor cells from pluripotent stem cells

Publications (1)

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BR112022003089A2 true BR112022003089A2 (pt) 2022-05-17

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BR112022003089A BR112022003089A2 (pt) 2019-08-20 2020-08-20 Métodos de produção de células progenitoras hemogênicas a partir de células-tronco pluripotentes

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Country Link
US (1) US20220267733A1 (https=)
EP (1) EP4017967A1 (https=)
JP (1) JP2022545782A (https=)
KR (1) KR20220049032A (https=)
CN (1) CN114269906A (https=)
AU (1) AU2020332863A1 (https=)
BR (1) BR112022003089A2 (https=)
CA (1) CA3150427A1 (https=)
GB (1) GB201911957D0 (https=)
IL (1) IL290605A (https=)
MX (1) MX2022001960A (https=)
WO (1) WO2021032851A1 (https=)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11591381B2 (en) 2020-11-30 2023-02-28 Crispr Therapeutics Ag Gene-edited natural killer cells
WO2022144632A1 (en) 2020-12-30 2022-07-07 Crispr Therapeutics Ag Compositions and methods for differentiating stem cells into nk cells
GB202102297D0 (en) * 2021-02-18 2021-04-07 Adaptimmune Ltd Methods of Producing Haemogenic Endothelial Cells from Pluripotent Stem Cells
CN113046318B (zh) * 2021-04-13 2023-04-18 中国科学院深圳先进技术研究院 一种诱导多能干细胞向造血前体细胞分化的培养基以及方法
EP4326852A4 (en) * 2021-04-22 2025-04-23 Artec Biotech, Inc. Method for producing hematopoietic cells from stem cells using vascular organoids
CN113265377B (zh) * 2021-06-11 2023-01-24 中国医学科学院血液病医院(中国医学科学院血液学研究所) 一种动脉内皮促进功能性t细胞再生的方法
JP2024531626A (ja) * 2021-09-10 2024-08-29 スンクワン メディカル ファウンデーション 分化全能性幹細胞由来の造血幹細胞の製造方法、及び製造された造血幹細胞を利用したヒト化マウスモデル作製方法
CN116410927A (zh) * 2021-12-31 2023-07-11 士泽生物医药（苏州）有限公司 干细胞诱导分化为造血祖细胞的方法
CN116376822A (zh) * 2021-12-31 2023-07-04 北京大学 利用人多潜能干细胞体外制备t淋巴细胞
TW202340453A (zh) * 2022-01-28 2023-10-16 中國大陸商杭州啟函生物科技有限公司 用於基於細胞的免疫療法的方法和組合物
GB202205572D0 (en) 2022-04-14 2022-06-01 Adaptimmune Ltd Engineered T cells
GB202208545D0 (en) 2022-06-10 2022-07-27 Adaptimmune Ltd Production of immune cells
CN114958771B (zh) * 2022-06-27 2023-12-05 广东康盾创新产业集团股份公司 一种ipsc-car-nk细胞的制备方法
CN115247151B (zh) * 2022-09-21 2022-12-27 呈诺再生医学科技(北京)有限公司 制备造血内皮细胞的方法及制备造血干细胞或造血干祖细胞的方法
KR20240054468A (ko) * 2022-10-18 2024-04-26 의료법인 성광의료재단 줄기세포에서 대식세포로의 분화 유도용 배지 조성물 및 이를 이용한 분화 방법
US20240316196A1 (en) * 2022-12-02 2024-09-26 Nuwacell Biotechnologies Co., Ltd. Methods and compositions for differentiation of pluripotent stem cells and derived natural killer cells
CN117264888A (zh) * 2023-10-11 2023-12-22 苏州艾凯利元生物科技有限公司 一种制备造血干细胞和祖细胞的方法
GB202318950D0 (en) 2023-12-12 2024-01-24 Adaptimmune Ltd Production of immune cells

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008151390A1 (en) * 2007-06-15 2008-12-18 Australian Stem Cell Centre Ltd Differentiation of human embryonic stem cells
JP5777115B2 (ja) * 2010-03-18 2015-09-09 国立大学法人京都大学 多能性幹細胞から中胚葉細胞への分化誘導法
JP6677631B2 (ja) * 2013-03-13 2020-04-08 ウイスコンシン アラムナイ リサーチ ファウンデーシヨンＷｉｓｃｏｎｓｉｎ Ａｌｕｍｎｉ Ｒｅｓｅａｒｃｈ Ｆｏｕｎｄａｔｉｏｎ 定義された条件下におけるヒト多能性幹細胞の造血内皮分化のための方法及び材料
CA2925774A1 (en) * 2013-10-01 2015-04-09 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Human ipsc-derived vascular-related and hematopoetic cells for therapies and toxicology/drug screenings
AU2016211671B2 (en) * 2015-01-26 2022-05-26 Fate Therapeutics, Inc. Methods and compositions for inducing hematopoietic cell differentiation
CA2975268A1 (en) * 2015-02-20 2016-08-25 Wisconsin Alumni Research Foundation Generating arterial endothelial cell populations
JP6928604B2 (ja) * 2015-11-04 2021-09-01 フェイト セラピューティクス，インコーポレイテッド 万能性細胞のゲノム改変
CA3003152A1 (en) * 2015-11-04 2017-05-11 Fate Therapeutics, Inc. Methods and compositions for inducing hematopoietic cell differentiation
US11261430B2 (en) * 2016-05-03 2022-03-01 The Children's Medical Center Corporation Hematopoietic stem and progenitor cells derived from hemogenic endothelial cells
EP3621630A4 (en) * 2017-06-14 2021-03-10 The Children's Medical Center HEMATOPOIETIC STEM AND PRECURSOR CELLS FROM HEMOGENIC ENDOTHELIAL CELLS BY EPISOMAL PLASMID GENERAL TRANSFER
SG11202006731VA (en) * 2018-01-18 2020-08-28 Agency Science Tech & Res Method for differentiation of human pluripotent stem cell lines in suspension culture
GB202006903D0 (en) * 2020-05-11 2020-06-24 Adaptimmune Ltd Modified iPSCs

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CN114269906A (zh) 2022-04-01
MX2022001960A (es) 2022-03-11
EP4017967A1 (en) 2022-06-29
US20220267733A1 (en) 2022-08-25
GB201911957D0 (en) 2019-10-02
IL290605A (en) 2022-04-01
WO2021032851A1 (en) 2021-02-25
AU2020332863A1 (en) 2022-03-24
KR20220049032A (ko) 2022-04-20
JP2022545782A (ja) 2022-10-31
CA3150427A1 (en) 2021-02-25

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