BR112021020143A2 - Integração de construções de ácidos nucleicos com células eucarióticas com uma transposase de oryzias - Google Patents

Integração de construções de ácidos nucleicos com células eucarióticas com uma transposase de oryzias

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Publication number
BR112021020143A2
BR112021020143A2 BR112021020143A BR112021020143A BR112021020143A2 BR 112021020143 A2 BR112021020143 A2 BR 112021020143A2 BR 112021020143 A BR112021020143 A BR 112021020143A BR 112021020143 A BR112021020143 A BR 112021020143A BR 112021020143 A2 BR112021020143 A2 BR 112021020143A2
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Prior art keywords
oryzia
transposase
integration
nucleic acid
eukaryotic cells
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BR112021020143A
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English (en)
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Jeremy Minshull
Maggie Lee
Sridhar Govindarajan
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Dna Twopointo Inc
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Publication date
Application filed by Dna Twopointo Inc filed Critical Dna Twopointo Inc
Publication of BR112021020143A2 publication Critical patent/BR112021020143A2/pt

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Abstract

ntegração de construções de ácidos nucleicos com células eucarióticas com uma transposase de oryzia. a presente invenção fornece vetores polinucleotídicos para alta expressão de genes heterólogos. alguns vetores compreendem adicionalmente novos transposons e transposases que melhoram ainda mais a expressão. além disso, são divulgados vetores que podem ser usados em um sistema de transferência de genes para a introdução estável de ácidos nucleicos no dna de uma célula. os sistemas de transferência de genes podem ser usados em métodos, por exemplo, expressão gênica, bioprocessamento, terapia gênica, mutagênese por inserção ou descoberta de genes.
BR112021020143A 2019-04-08 2020-04-07 Integração de construções de ácidos nucleicos com células eucarióticas com uma transposase de oryzias BR112021020143A2 (pt)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201962831092P 2019-04-08 2019-04-08
US201962873338P 2019-07-12 2019-07-12
US202062982186P 2020-02-27 2020-02-27
PCT/US2020/027080 WO2020210239A1 (en) 2019-04-08 2020-04-07 Integration of nucleic acid constructs into eukaryotic cells with a transposase from oryzias

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BR112021020143A2 true BR112021020143A2 (pt) 2021-12-14

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US (3) US11060098B2 (pt)
EP (1) EP3953481A4 (pt)
JP (1) JP7418470B2 (pt)
KR (1) KR20210143897A (pt)
CN (1) CN112424362A (pt)
AU (1) AU2020272668B2 (pt)
BR (1) BR112021020143A2 (pt)
CA (1) CA3125047A1 (pt)
IL (2) IL301620A (pt)
SG (1) SG11202107416PA (pt)
WO (1) WO2020210239A1 (pt)

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