BR112021020143A2 - Integração de construções de ácidos nucleicos com células eucarióticas com uma transposase de oryzias - Google Patents

Integração de construções de ácidos nucleicos com células eucarióticas com uma transposase de oryzias

Info

Publication number
BR112021020143A2
BR112021020143A2 BR112021020143A BR112021020143A BR112021020143A2 BR 112021020143 A2 BR112021020143 A2 BR 112021020143A2 BR 112021020143 A BR112021020143 A BR 112021020143A BR 112021020143 A BR112021020143 A BR 112021020143A BR 112021020143 A2 BR112021020143 A2 BR 112021020143A2
Authority
BR
Brazil
Prior art keywords
oryzia
transposase
integration
nucleic acid
eukaryotic cells
Prior art date
Application number
BR112021020143A
Other languages
English (en)
Inventor
Jeremy Minshull
Maggie Lee
Sridhar Govindarajan
Original Assignee
Dna Twopointo Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dna Twopointo Inc filed Critical Dna Twopointo Inc
Publication of BR112021020143A2 publication Critical patent/BR112021020143A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
    • C12N9/0028—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with NAD or NADP as acceptor (1.5.1)
    • C12N9/003—Dihydrofolate reductase [DHFR] (1.5.1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y105/00—Oxidoreductases acting on the CH-NH group of donors (1.5)
    • C12Y105/01—Oxidoreductases acting on the CH-NH group of donors (1.5) with NAD+ or NADP+ as acceptor (1.5.1)
    • C12Y105/01003—Dihydrofolate reductase (1.5.1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01119—Hygromycin-B 7''-O-kinase (2.7.1.119)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
    • C12Y603/01—Acid-ammonia (or amine)ligases (amide synthases)(6.3.1)
    • C12Y603/01002—Glutamate-ammonia ligase (6.3.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/90—Vectors containing a transposable element
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/007—Vector systems having a special element relevant for transcription cell cycle specific enhancer/promoter combination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Virology (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

ntegração de construções de ácidos nucleicos com células eucarióticas com uma transposase de oryzia. a presente invenção fornece vetores polinucleotídicos para alta expressão de genes heterólogos. alguns vetores compreendem adicionalmente novos transposons e transposases que melhoram ainda mais a expressão. além disso, são divulgados vetores que podem ser usados em um sistema de transferência de genes para a introdução estável de ácidos nucleicos no dna de uma célula. os sistemas de transferência de genes podem ser usados em métodos, por exemplo, expressão gênica, bioprocessamento, terapia gênica, mutagênese por inserção ou descoberta de genes.
BR112021020143A 2019-04-08 2020-04-07 Integração de construções de ácidos nucleicos com células eucarióticas com uma transposase de oryzias BR112021020143A2 (pt)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201962831092P 2019-04-08 2019-04-08
US201962873338P 2019-07-12 2019-07-12
US202062982186P 2020-02-27 2020-02-27
PCT/US2020/027080 WO2020210239A1 (en) 2019-04-08 2020-04-07 Integration of nucleic acid constructs into eukaryotic cells with a transposase from oryzias

Publications (1)

Publication Number Publication Date
BR112021020143A2 true BR112021020143A2 (pt) 2021-12-14

Family

ID=72663493

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112021020143A BR112021020143A2 (pt) 2019-04-08 2020-04-07 Integração de construções de ácidos nucleicos com células eucarióticas com uma transposase de oryzias

Country Status (11)

Country Link
US (3) US11060098B2 (pt)
EP (1) EP3953481A4 (pt)
JP (1) JP7418470B2 (pt)
KR (1) KR20210143897A (pt)
CN (1) CN112424362A (pt)
AU (1) AU2020272668B2 (pt)
BR (1) BR112021020143A2 (pt)
CA (1) CA3125047A1 (pt)
IL (2) IL287089B2 (pt)
SG (1) SG11202107416PA (pt)
WO (1) WO2020210239A1 (pt)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2311490A3 (en) 1998-07-13 2011-05-04 Board of Regents, The University of Texas System Uses of antibodies to aminophospholipids for cancer treatment
IL301621A (en) * 2019-04-08 2023-05-01 Dna Twopointo Inc Transposition of nucleic acid constructs into eukaryotic genomes with amylase transposases
CN112424362A (zh) 2019-04-08 2021-02-26 Dna2.0股份有限公司 使用来自青鳉属的转座酶将核酸构建体整合入真核细胞
EP3953480A4 (en) 2019-04-08 2023-01-11 Dna Twopointo Inc. TRANSPOSITION OF NUCLEIC ACIDS INTO EUKARYOTIC GENOMES WITH A TRANSPOSASE FROM HELIOTHIS
WO2021226141A1 (en) 2020-05-04 2021-11-11 Saliogen Therapeutics, Inc. Transposition-based therapies
US11566262B2 (en) * 2021-03-24 2023-01-31 Dna Twopointo Inc. Tetracycline-inducible expression systems
WO2023060089A2 (en) * 2021-10-04 2023-04-13 Poseida Therapeutics, Inc. Transposases and uses thereof
CN114045305B (zh) * 2021-10-15 2023-03-24 深圳市深研生物科技有限公司 多转座子系统
CN116445542A (zh) * 2022-01-10 2023-07-18 佛山汉腾生物科技有限公司 表达载体及其应用
CN115521899A (zh) * 2022-05-06 2022-12-27 西南大学 一种高效的日本青鱂体外培养细胞CRISPR/Cas9基因组编辑方法及其应用
WO2024068995A1 (en) 2022-09-30 2024-04-04 Boehringer Ingelheim International Gmbh Novel transposase system

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7560542B2 (en) * 1999-05-07 2009-07-14 Monsanto Technology Llc Nucleic acid molecule SEQ ID NO. 68811 and other molecules associated with plants
JP4629162B2 (ja) * 1999-12-03 2011-02-09 独立行政法人科学技術振興機構 トランスポゾン転移酵素および遺伝子改変方法
US7932088B1 (en) 2005-04-25 2011-04-26 University Of Notre Dame Du Lac High efficiency transformation of Plasmodium falciparum by the lepidopteran transposon, piggyBac
WO2006122442A1 (en) * 2005-05-14 2006-11-23 Fudan University Piggybac as a tool for genetic manipulation and analysis in vertebrates
JP4588681B2 (ja) * 2006-09-15 2010-12-01 株式会社日立国際電気 無線基地局
JP5320546B2 (ja) * 2006-12-13 2013-10-23 国立大学法人名古屋大学 Ｔｏｌ１因子のトランスポザーゼ及びそれを用いたＤＮＡ導入システム
EP2160461B1 (en) * 2007-07-04 2012-08-08 Max-Delbrück-Centrum für Molekulare Medizin (MDC) Hyperactive variants of the transposase protein of the transposon system sleeping beauty
JP5897335B2 (ja) * 2009-01-23 2016-03-30 エヌエスジーン・アクティーゼルスカブＮｓＧｅｎｅ Ａ／Ｓ 改良された細胞株及びカプセル化細胞生体送達におけるその使用
SG174155A1 (en) 2009-02-25 2011-10-28 Univ Johns Hopkins Piggybac transposon variants and methods of use
CN106978441A (zh) * 2009-06-11 2017-07-25 大学共同利用机关法人情报·系统研究机构 生产蛋白质的方法
AU2013204327B2 (en) * 2012-04-20 2016-09-01 Aviagen Cell transfection method
JP2014230515A (ja) * 2013-05-29 2014-12-11 国立大学法人 東京大学 新規なトランスポゾン転移酵素
US10233454B2 (en) * 2014-04-09 2019-03-19 Dna2.0, Inc. DNA vectors, transposons and transposases for eukaryotic genome modification
US9428767B2 (en) * 2014-04-09 2016-08-30 Dna2.0, Inc. Enhanced nucleic acid constructs for eukaryotic gene expression
EP3359671B1 (en) * 2015-10-08 2021-08-18 Dna Twopointo Inc. Dna vectors, transposons and transposases for eukaryotic genome modification
IL301621A (en) * 2019-04-08 2023-05-01 Dna Twopointo Inc Transposition of nucleic acid constructs into eukaryotic genomes with amylase transposases
CN112424362A (zh) 2019-04-08 2021-02-26 Dna2.0股份有限公司 使用来自青鳉属的转座酶将核酸构建体整合入真核细胞
EP3953480A4 (en) 2019-04-08 2023-01-11 Dna Twopointo Inc. TRANSPOSITION OF NUCLEIC ACIDS INTO EUKARYOTIC GENOMES WITH A TRANSPOSASE FROM HELIOTHIS
EP3969462A4 (en) * 2019-05-13 2023-01-18 Dna Twopointo Inc. MODIFICATIONS OF MAMMALIAN CELLS USING ARTIFICIAL MICRORNA TO MODIFY THEIR PROPERTIES AND COMPOSITIONS OF THEIR PRODUCTS
CN117015600A (zh) * 2020-07-17 2023-11-07 普罗拜奥根股份公司 高活性转座子和转座酶

Also Published As

Publication number Publication date
JP2022527017A (ja) 2022-05-27
IL301620A (en) 2023-05-01
CA3125047A1 (en) 2020-10-15
IL287089B1 (en) 2023-05-01
US11060098B2 (en) 2021-07-13
WO2020210239A1 (en) 2020-10-15
AU2020272668A1 (en) 2021-07-15
IL287089B2 (en) 2023-09-01
EP3953481A4 (en) 2023-01-25
US20210292773A1 (en) 2021-09-23
KR20210143897A (ko) 2021-11-29
EP3953481A1 (en) 2022-02-16
US20230103199A1 (en) 2023-03-30
AU2020272668B2 (en) 2023-07-06
CN112424362A (zh) 2021-02-26
US20200318121A1 (en) 2020-10-08
IL287089A (en) 2021-12-01
US11401521B2 (en) 2022-08-02
JP7418470B2 (ja) 2024-01-19
SG11202107416PA (en) 2021-08-30

Similar Documents

Publication Publication Date Title
BR112021020143A2 (pt) Integração de construções de ácidos nucleicos com células eucarióticas com uma transposase de oryzias
BR112021020144A2 (pt) Transposição de construções de ácidos nucleicos em genomas eucarióticos com uma transposase de amyelois
US11913040B2 (en) Evolution of TALENs
BR112021012665A2 (pt) Polipeptídeos úteis para edição de genes e métodos de uso
WO2017062668A3 (en) Dna vectors, transposons and transposases for eukaryotic genome modification
US9863933B2 (en) Method for determining the repair activity of non-homologous end joining
JP2023002712A (ja) Ｓ．ピオゲネスｃａｓ９変異遺伝子及びこれによってコードされるポリペプチド
US11242542B2 (en) S. pyogenes Cas9 mutant genes and polypeptides encoded by same
EP4321617A3 (en) Crispr/cpf1 systems and methods
BR112020024863A2 (pt) Nucleases guiadas por rna, fragmentos ativos e variantes das mesmas e métodos de uso
CL2021002742A1 (es) Una proteína crispr de prevotella y francisella 1 (cpf1) aislada de francisella tularensis; proteína de fusión que la comprende; ácido nucleico; vector; métodos para alterar el genoma de una célula o una molécula de adn bicatenario in vitro que utilizan dichas proteínas (divisional de sol. 201903004)
BR112019012183A2 (pt) métodos de modificar o material genético de uma célula eucariótica e de um organismo, vetor de expressão de polinucleotídeo, e, célula procariótica.
US20170349905A1 (en) Genome editing with split cas9 expressed from two vectors
Chen et al. CRISPR-Cas9: from genome editing to cancer research
EA201892810A1 (ru) Гибридные последовательности нуклеиновых кислот для геномной инженерии
BR112019006388A2 (pt) enzimas modificadoras de ácido nucleico guiadas por rna e métodos de uso das mesmas
WO2018188571A1 (zh) 基因组编辑系统和方法
ES2690386T3 (es) Composición para escindir un ADN diana que comprende un ARN guía específico para el ADN diana y el ácido nucleico que codifica la proteína Cas o la propia proteína Cas, y sus usos
WO2010085699A3 (en) Mammalian piggybac transposon and methods of use
Zhang et al. Improving the specificity and efficacy of CRISPR/CAS9 and gRNA through target specific DNA reporter
BR112022002695A2 (pt) Nucleases guiadas por rna, fragmentos ativos e variantes das mesmas e métodos de uso
BR112018067536A2 (pt) sistema de transpóson e métodos de uso
MX2019014516A (es) Metodo para eliminar el gen objetivo en celulas t in vitro y arncr utilizado en el metodo.
Lee et al. Protein–DNA photo-crosslinking with a genetically encoded benzophenone-containing amino acid
BR112018016443A2 (pt) sistema de transposon replicativo