SG11202110935RA - Modifications of mammalian cells using artificial micro-rna to alter their properties and the compositions of their products - Google Patents

Modifications of mammalian cells using artificial micro-rna to alter their properties and the compositions of their products

Info

Publication number
SG11202110935RA
SG11202110935RA SG11202110935RA SG11202110935RA SG11202110935RA SG 11202110935R A SG11202110935R A SG 11202110935RA SG 11202110935R A SG11202110935R A SG 11202110935RA SG 11202110935R A SG11202110935R A SG 11202110935RA SG 11202110935R A SG11202110935R A SG 11202110935RA
Authority
SG
Singapore
Prior art keywords
rna
alter
compositions
properties
products
Prior art date
Application number
SG11202110935RA
Inventor
Jeremy Minshull
Maggie Lee
Varsha Sitaraman
Ferenc Boldog
Original Assignee
Dna Twopointo Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dna Twopointo Inc filed Critical Dna Twopointo Inc
Publication of SG11202110935RA publication Critical patent/SG11202110935RA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1247—DNA-directed RNA polymerase (2.7.7.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
    • C12Y603/01—Acid-ammonia (or amine)ligases (amide synthases)(6.3.1)
    • C12Y603/01002—Glutamate-ammonia ligase (6.3.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C12N2310/141—MicroRNAs, miRNAs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3519—Fusion with another nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/51—Physical structure in polymeric form, e.g. multimers, concatemers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/531—Stem-loop; Hairpin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/90—Vectors containing a transposable element
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07006—DNA-directed RNA polymerase (2.7.7.6)
SG11202110935RA 2019-05-13 2020-05-11 Modifications of mammalian cells using artificial micro-rna to alter their properties and the compositions of their products SG11202110935RA (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201962846847P 2019-05-13 2019-05-13
US201962870321P 2019-07-03 2019-07-03
US202062981417P 2020-02-25 2020-02-25
US202063019733P 2020-05-04 2020-05-04
PCT/US2020/032381 WO2020231943A1 (en) 2019-05-13 2020-05-11 Modifications of mammalian cells using artificial micro-rna to alter their properties and the compositions of their products

Publications (1)

Publication Number Publication Date
SG11202110935RA true SG11202110935RA (en) 2021-10-28

Family

ID=73231101

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202110935RA SG11202110935RA (en) 2019-05-13 2020-05-11 Modifications of mammalian cells using artificial micro-rna to alter their properties and the compositions of their products

Country Status (10)

Country Link
US (4) US11162102B2 (en)
EP (1) EP3969462A4 (en)
JP (1) JP7284833B2 (en)
KR (1) KR20220002609A (en)
CN (1) CN114127083A (en)
AU (1) AU2020274339C1 (en)
CA (1) CA3136470A1 (en)
IL (1) IL287969A (en)
SG (1) SG11202110935RA (en)
WO (1) WO2020231943A1 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL287089B2 (en) * 2019-04-08 2023-09-01 Dna Twopointo Inc Integration of nucleic acid constructs into eukaryotic cells with a transposase from oryzias
WO2020231943A1 (en) 2019-05-13 2020-11-19 Dna Twopointo Inc. Modifications of mammalian cells using artificial micro-rna to alter their properties and the compositions of their products
WO2021226141A1 (en) 2020-05-04 2021-11-11 Saliogen Therapeutics, Inc. Transposition-based therapies
KR102602485B1 (en) * 2021-07-09 2023-11-17 주식회사 네오젠티씨 Transposon system and its use
WO2023064928A2 (en) * 2021-10-14 2023-04-20 Arsenal Biosciences, Inc. Immune cells having co-expressed shrnas and logic gate systems
WO2023081814A2 (en) * 2021-11-04 2023-05-11 Saliogen Therapeutics, Inc. Mobile elements and chimeric constructs thereof
WO2023086882A1 (en) * 2021-11-11 2023-05-19 The Trustees Of The University Of Pennsylvania Compositions and methods comprising car t cells comprising prdm1 and/or nr4a3 knockout
WO2023152498A1 (en) * 2022-02-09 2023-08-17 Horizon Discovery Limited Polynucleotides with selection markers

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3184632B1 (en) * 2009-02-26 2024-04-03 Poseida Therapeutics, Inc. Hyperactive piggybac transposases
WO2010140862A2 (en) * 2009-06-05 2010-12-09 Seol Dai-Wu MULTI-CISTRONIC shRNA EXPRESSION CASSETTE FOR SUPPRESSING SINGLE OR MULTIPLE TARGET GENES
BR112012000421A2 (en) 2009-07-06 2019-09-24 Alnylam Pharmaceuticals Inc compositions and methods for enhancing the production of a biological product.
WO2011119901A1 (en) * 2010-03-26 2011-09-29 Alnylam Pharmaceuticals, Inc. Gene amplification and transfection methods and reagents related thereto
DK2694644T3 (en) * 2011-03-30 2018-04-16 Cellular Dynamics Int Inc Priming of pluripotent stem cells for neural differentiation
WO2013013013A2 (en) 2011-07-21 2013-01-24 Alnylam Pharmaceuticals, Inc. Compositions and methods for producing modified glycoproteins
US20150089689A1 (en) * 2012-01-23 2015-03-26 E I Du Pont Nemours And Company Down-regulation of gene expression using artificial micrornas for silencing fatty acid biosynthetic genes
AU2013216703A1 (en) * 2012-02-10 2014-09-25 Whitehead Institute For Biomedical Research Inhibition of the glycine cleavage system for treatment of cancer
WO2014072357A1 (en) 2012-11-06 2014-05-15 Interna Technologies B.V. Combination for use in treating diseases or conditions associated with melanoma, or treating diseases or conditions associated with activated b-raf pathway
US20140271926A1 (en) * 2013-03-12 2014-09-18 California Institute Of Technology Methods of use of glutamine synthetase inhibitors
US10927384B2 (en) * 2014-04-09 2021-02-23 Dna Twopointo Inc. DNA vectors, transposons and transposases for eukaryotic genome modification
HUE052552T2 (en) * 2014-04-09 2021-05-28 Dna Twopointo Inc Enhanced nucleic acid constructs for eukaryotic gene expression
CN107405357B (en) 2014-10-14 2021-12-31 德克萨斯科技大学系统 Multiple shRNAs and application thereof
JP2018509164A (en) 2015-03-10 2018-04-05 ザ・トラスティーズ・オブ・コロンビア・ユニバーシティ・イン・ザ・シティ・オブ・ニューヨーク Recombinant Glut1 adeno-associated virus vector construct and method for restoring Glut1 expression
CN107893073B (en) 2017-11-14 2020-06-02 深圳市深研生物科技有限公司 Method for screening glutamine synthetase defect type HEK293 cell strain
EP3569713A1 (en) 2018-05-16 2019-11-20 Jennewein Biotechnologie GmbH Use of glycosidases in the production of oligosaccharides
WO2020231943A1 (en) 2019-05-13 2020-11-19 Dna Twopointo Inc. Modifications of mammalian cells using artificial micro-rna to alter their properties and the compositions of their products

Also Published As

Publication number Publication date
JP7284833B2 (en) 2023-05-31
US11162102B2 (en) 2021-11-02
WO2020231943A1 (en) 2020-11-19
IL287969A (en) 2022-01-01
AU2020274339A1 (en) 2021-11-11
AU2020274339B2 (en) 2023-02-23
US11845936B2 (en) 2023-12-19
US20220112500A1 (en) 2022-04-14
AU2020274339C1 (en) 2023-08-31
CN114127083A (en) 2022-03-01
EP3969462A1 (en) 2022-03-23
US20200362344A1 (en) 2020-11-19
KR20220002609A (en) 2022-01-06
CA3136470A1 (en) 2020-11-19
US20240093196A1 (en) 2024-03-21
US20230295625A1 (en) 2023-09-21
JP2022532357A (en) 2022-07-14
EP3969462A4 (en) 2023-01-18

Similar Documents

Publication Publication Date Title
IL287969A (en) Modifications of mammalian cells using artificial micro-rna to alter their properties and the compositions of their products
EP3638777A4 (en) Compositions and methods for increasing the efficiency of cell cultures used for food production
ZA202000840B (en) Generation of water-soluble cannabinoid compounds in yeast and plant cell suspension cultures and compositions of matter
IL288159A (en) Devices, methods, and compositions useful in cryo-preservation, -storage, -transport, and application of therapeutic mammalian cells
EP3793579A4 (en) Methods and compositions for generating cells of endodermal lineage and beta cells and uses thereof
IL290862A (en) Compositions and methods for delivery of nucleic acids to cells
EP3538160A4 (en) Methods and compositions high scale therapeutic production of memory-like nk cells
EP3737424A4 (en) Compositions and methods for facilitating delivery of synthetic nucleic acids to cells
SG11202102431YA (en) Organoid compositions for the production of hematopoietic stem cells and derivatives thereof
DK3837288T1 (en) Activated pectin-containing biomass compositions, products, and methods of producing
MA49981A (en) PROCESSES AND COMPOSITIONS FOR THE PREPARATION OF GENETICALLY MODIFIED CELLS
EP4048296A4 (en) Method of producing natural killer cells and compositions thereof
EP3452578A4 (en) Methods for the in vitro manufacture of gastric fundus tissue and compositions related to same
IL262195A (en) Methods and compositions to enhance the anti-inflammatory effects of interleukin 10
IL290297A (en) Modified cytotoxic t cells and methods of use thereof
EP3853381A4 (en) Phenotypic and molecular characterisation of single cells
EP3976070A4 (en) Shaped organoid compositions and methods of making same
GB202111772D0 (en) Artificial honey composition and production process
IL286695A (en) Modulators of cell surface protein interactions and methods and compositions related to same
WO2016201133A3 (en) Hematopoietic cells and methods of using and generating the same
EP3551749A4 (en) Compositions and methods for in vitro cultivation and/or expansion of regulatory t cells
EP3880827A4 (en) Compositions and methods of inducing differentiation of a hair cell
GB201821286D0 (en) Compositions and methods for delivery of nucleic acid to plant cells
IL287010A (en) Compositions and methods for the cryopreservation of immune cells
WO2022051627A3 (en) Modifications of mammalian cells using artificial micro-rna to alter their properties and the compositions of their products