SG11202110935RA - Modifications of mammalian cells using artificial micro-rna to alter their properties and the compositions of their products - Google Patents

Modifications of mammalian cells using artificial micro-rna to alter their properties and the compositions of their products

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Publication number
SG11202110935RA
SG11202110935RA SG11202110935RA SG11202110935RA SG11202110935RA SG 11202110935R A SG11202110935R A SG 11202110935RA SG 11202110935R A SG11202110935R A SG 11202110935RA SG 11202110935R A SG11202110935R A SG 11202110935RA SG 11202110935R A SG11202110935R A SG 11202110935RA
Authority
SG
Singapore
Prior art keywords
rna
alter
compositions
properties
products
Prior art date
Application number
SG11202110935RA
Inventor
Jeremy Minshull
Maggie Lee
Varsha Sitaraman
Ferenc Boldog
Original Assignee
Dna Twopointo Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dna Twopointo Inc filed Critical Dna Twopointo Inc
Publication of SG11202110935RA publication Critical patent/SG11202110935RA/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1247—DNA-directed RNA polymerase (2.7.7.6)
    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
    • C12Y603/01—Acid-ammonia (or amine)ligases (amide synthases)(6.3.1)
    • C12Y603/01002—Glutamate-ammonia ligase (6.3.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C12N2310/141—MicroRNAs, miRNAs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3519—Fusion with another nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/51—Physical structure in polymeric form, e.g. multimers, concatemers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/531—Stem-loop; Hairpin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/90—Vectors containing a transposable element
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07006—DNA-directed RNA polymerase (2.7.7.6)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
SG11202110935RA 2019-05-13 2020-05-11 Modifications of mammalian cells using artificial micro-rna to alter their properties and the compositions of their products SG11202110935RA (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201962846847P 2019-05-13 2019-05-13
US201962870321P 2019-07-03 2019-07-03
US202062981417P 2020-02-25 2020-02-25
US202063019733P 2020-05-04 2020-05-04
PCT/US2020/032381 WO2020231943A1 (en) 2019-05-13 2020-05-11 Modifications of mammalian cells using artificial micro-rna to alter their properties and the compositions of their products

Publications (1)

Publication Number Publication Date
SG11202110935RA true SG11202110935RA (en) 2021-10-28

Family

ID=73231101

Family Applications (1)

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SG11202110935RA SG11202110935RA (en) 2019-05-13 2020-05-11 Modifications of mammalian cells using artificial micro-rna to alter their properties and the compositions of their products

Country Status (10)

Country Link
US (4) US11162102B2 (en)
EP (1) EP3969462A4 (en)
JP (1) JP7284833B2 (en)
KR (1) KR20220002609A (en)
CN (1) CN114127083A (en)
AU (1) AU2020274339C1 (en)
CA (1) CA3136470A1 (en)
IL (1) IL287969A (en)
SG (1) SG11202110935RA (en)
WO (1) WO2020231943A1 (en)

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KR20210143897A (en) * 2019-04-08 2021-11-29 디앤에이 투포인토 인크. Integration of Nucleic Acid Constructs into Eukaryotic Cells Using Transposase from Origias
SG11202110935RA (en) 2019-05-13 2021-10-28 Dna Twopointo Inc Modifications of mammalian cells using artificial micro-rna to alter their properties and the compositions of their products
CA3173889A1 (en) 2020-05-04 2021-11-11 Saliogen Therapeutics, Inc. Transposition-based therapies
EP4368719A1 (en) * 2021-07-09 2024-05-15 Neogentc Corp. Transposon system and uses thereof
AU2022366987A1 (en) * 2021-10-14 2024-05-16 Arsenal Biosciences, Inc. Immune cells having co-expressed shrnas and logic gate systems
CA3236684A1 (en) * 2021-11-04 2023-05-11 Joseph J. HIGGINS Mobile elements and chimeric constructs thereof
WO2023086882A1 (en) * 2021-11-11 2023-05-19 The Trustees Of The University Of Pennsylvania Compositions and methods comprising car t cells comprising prdm1 and/or nr4a3 knockout
WO2023152498A1 (en) * 2022-02-09 2023-08-17 Horizon Discovery Limited Polynucleotides with selection markers
WO2024123813A1 (en) * 2022-12-05 2024-06-13 The Trustees Of Columbia University In The City Of New York Viral vectors for increasing the specificity of gene expression

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WO2010099296A1 (en) * 2009-02-26 2010-09-02 Transposagen Biopharmaceuticals, Inc. Hyperactive piggybac transposases
US20120142764A1 (en) * 2009-06-05 2012-06-07 Dai-Wu Seol Multi-Cistronic shRNA Expression Cassette for Suppressing Single or Multiple Target Genes
US20140099666A1 (en) 2009-07-06 2014-04-10 Alnylam Pharmaceuticals, Inc. Compositions and methods for enhancing production of a biological product
WO2011119901A1 (en) * 2010-03-26 2011-09-29 Alnylam Pharmaceuticals, Inc. Gene amplification and transfection methods and reagents related thereto
WO2012135621A2 (en) * 2011-03-30 2012-10-04 Cellular Dynamics International. Inc Priming of pluripotent stem cells for neural differentiation
WO2013013013A2 (en) 2011-07-21 2013-01-24 Alnylam Pharmaceuticals, Inc. Compositions and methods for producing modified glycoproteins
ES2635115T3 (en) * 2012-01-23 2017-10-02 E. I. Du Pont De Nemours And Company Regulation of decreased gene expression using artificial micro RNAs to silence biosynthetic fatty acid genes
NZ630026A (en) * 2012-02-10 2016-06-24 Whitehead Biomedical Inst Inhibition of the glycine cleavage system for treatment of cancer
US10201556B2 (en) 2012-11-06 2019-02-12 Interna Technologies B.V. Combination for use in treating diseases or conditions associated with melanoma, or treating diseases or conditions associated with activated B-raf pathway
US20140271926A1 (en) * 2013-03-12 2014-09-18 California Institute Of Technology Methods of use of glutamine synthetase inhibitors
US10041077B2 (en) * 2014-04-09 2018-08-07 Dna2.0, Inc. DNA vectors, transposons and transposases for eukaryotic genome modification
DK3129487T3 (en) * 2014-04-09 2020-11-30 Dna Twopointo Inc IMPROVED NUCLEIC ACID CONSTRUCTIONS FOR EUKARYOT GENEPRESSION
EP3206696A4 (en) 2014-10-14 2018-08-08 Texas Tech University System Multiplexed shrnas and uses thereof
MX2017011615A (en) 2015-03-10 2018-04-11 Univ Columbia Recombinant glut1 adeno-associated viral vector constructs and related methods for restoring glut1 expression.
CN107893073B (en) * 2017-11-14 2020-06-02 深圳市深研生物科技有限公司 Method for screening glutamine synthetase defect type HEK293 cell strain
EP3569713A1 (en) 2018-05-16 2019-11-20 Jennewein Biotechnologie GmbH Use of glycosidases in the production of oligosaccharides
SG11202110935RA (en) 2019-05-13 2021-10-28 Dna Twopointo Inc Modifications of mammalian cells using artificial micro-rna to alter their properties and the compositions of their products

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CA3136470A1 (en) 2020-11-19
CN114127083A (en) 2022-03-01
US20200362344A1 (en) 2020-11-19
JP2022532357A (en) 2022-07-14
IL287969A (en) 2022-01-01
EP3969462A4 (en) 2023-01-18
AU2020274339A1 (en) 2021-11-11
KR20220002609A (en) 2022-01-06
JP7284833B2 (en) 2023-05-31
AU2020274339B2 (en) 2023-02-23
US20230295625A1 (en) 2023-09-21
US20240093196A1 (en) 2024-03-21
AU2020274339C1 (en) 2023-08-31
WO2020231943A1 (en) 2020-11-19
EP3969462A1 (en) 2022-03-23
US11845936B2 (en) 2023-12-19
US11162102B2 (en) 2021-11-02
US20220112500A1 (en) 2022-04-14

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