BR112021018819A2 - Multiplexar elementos regulatórios para identificar elementos regulatórios específicos do tipo de célula - Google Patents

Multiplexar elementos regulatórios para identificar elementos regulatórios específicos do tipo de célula

Info

Publication number
BR112021018819A2
BR112021018819A2 BR112021018819A BR112021018819A BR112021018819A2 BR 112021018819 A2 BR112021018819 A2 BR 112021018819A2 BR 112021018819 A BR112021018819 A BR 112021018819A BR 112021018819 A BR112021018819 A BR 112021018819A BR 112021018819 A2 BR112021018819 A2 BR 112021018819A2
Authority
BR
Brazil
Prior art keywords
regulatory elements
cell type
multiplex
identify cell
specific
Prior art date
Application number
BR112021018819A
Other languages
English (en)
Inventor
Anne Tanenhaus
S Chen Jerry
Kartik Ramamoorthi
Martin Moorhead
Raghavendra Hosur
Sirika Wood
Stephanie Tagliatela
Original Assignee
Encoded Therapeutics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Encoded Therapeutics Inc filed Critical Encoded Therapeutics Inc
Publication of BR112021018819A2 publication Critical patent/BR112021018819A2/pt

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5023—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on expression patterns
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

multiplexar elementos regulatórios para identificar elementos regulatórios específicos do tipo de célula. é fornecido aqui um método de alto rendimento para rastrear e identificar elementos reguladores que fornecem expressão seletiva em um tipo de célula de interesse particular. também são fornecidas composições de ácido nucleico usadas no método de triagem de alto rendimento.
BR112021018819A 2019-03-22 2020-03-20 Multiplexar elementos regulatórios para identificar elementos regulatórios específicos do tipo de célula BR112021018819A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962822528P 2019-03-22 2019-03-22
PCT/US2020/023881 WO2020198017A1 (en) 2019-03-22 2020-03-20 Multiplexing regulatory elements to identify cell-type specific regulatory elements

Publications (1)

Publication Number Publication Date
BR112021018819A2 true BR112021018819A2 (pt) 2021-11-23

Family

ID=72611757

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112021018819A BR112021018819A2 (pt) 2019-03-22 2020-03-20 Multiplexar elementos regulatórios para identificar elementos regulatórios específicos do tipo de célula

Country Status (17)

Country Link
US (1) US20220170910A1 (pt)
EP (1) EP3942055A4 (pt)
JP (1) JP2022525477A (pt)
KR (1) KR20210143855A (pt)
CN (1) CN113874515A (pt)
AU (1) AU2020245425A1 (pt)
BR (1) BR112021018819A2 (pt)
CA (1) CA3134501A1 (pt)
CL (1) CL2021002433A1 (pt)
CO (1) CO2021012576A2 (pt)
EA (1) EA202192580A1 (pt)
IL (1) IL286455A (pt)
MA (1) MA55386A (pt)
MX (1) MX2021011511A (pt)
SG (1) SG11202110298RA (pt)
TW (1) TW202102680A (pt)
WO (1) WO2020198017A1 (pt)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114085873A (zh) * 2021-11-16 2022-02-25 珠海中科先进技术研究院有限公司 一种癌细胞状态辨识基因电路组及其制备方法
WO2024005574A1 (ko) * 2022-06-29 2024-01-04 서울대학교산학협력단 Mrna 번역 증가용 조절 엘리먼트를 스크리닝하는 방법, 상기 방법에 따른 신규 조절 엘리먼트, 및 이의 용도

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9447411B2 (en) * 2013-01-14 2016-09-20 Cellecta, Inc. Methods and compositions for single cell expression profiling
AU2015364636B9 (en) * 2014-12-16 2021-12-02 Board Of Regents Of The University Of Nebraska Gene therapy for Juvenile Batten Disease
WO2016134337A1 (en) * 2015-02-19 2016-08-25 University Of Florida Research Foundation, Inc. Site-specific integrating recombinant aav vectors for gene therapy and improved production methods
MX2017014561A (es) * 2015-05-15 2018-03-02 Pioneer Hi Bred Int Nuevos sistemas de arn guia/endonucleasa cas.
WO2017070605A1 (en) * 2015-10-22 2017-04-27 The Broad Institute Inc. Type vi-b crispr enzymes and systems
SG11201909203WA (en) * 2017-04-03 2019-11-28 Encoded Therapeutics Inc Tissue selective transgene expression

Also Published As

Publication number Publication date
EP3942055A4 (en) 2022-12-28
TW202102680A (zh) 2021-01-16
IL286455A (en) 2021-12-01
CL2021002433A1 (es) 2022-09-20
US20220170910A1 (en) 2022-06-02
WO2020198017A1 (en) 2020-10-01
KR20210143855A (ko) 2021-11-29
EP3942055A1 (en) 2022-01-26
JP2022525477A (ja) 2022-05-16
MA55386A (fr) 2022-01-26
EA202192580A1 (ru) 2022-03-10
CA3134501A1 (en) 2020-10-01
MX2021011511A (es) 2022-01-31
CN113874515A (zh) 2021-12-31
SG11202110298RA (en) 2021-10-28
AU2020245425A1 (en) 2021-10-07
CO2021012576A2 (es) 2021-10-20

Similar Documents

Publication Publication Date Title
SG10201806890VA (en) Massively parallel single cell analysis
CO2018004763A2 (es) Edición genómica multiplexada
BR112017017025A2 (pt) métodos e composições para analizar componentes celulares
MX2016016904A (es) Analisis de secuencias de acidos nucleicos.
CO2017011957A2 (es) Variantes de alfa-amilasa y polinucleótidos que codifican las mismas
CL2018000490A1 (es) Anticuerpos anti-pd1 y sus métodos de uso
MX2016016902A (es) Metodos para analizar acidos nucleicos de celulas individuales o poblaciones de celulas.
CO2020003882A2 (es) Anticuerpo multiespecifico
CO7151523A2 (es) Método y composiciones para la modificación de adn objetivo dirigida por arn y para la modulación de la transcripción dirigida por arn
WO2014165267A3 (en) Systems and methods for prenatal genetic analysis
NZ740630A (en) Polymethine compounds and their use as fluorescent labels
BR112021018819A2 (pt) Multiplexar elementos regulatórios para identificar elementos regulatórios específicos do tipo de célula
AR093446A1 (es) Anticuerpos de antihemaglutinina y metodos de uso
BR122020018119A8 (pt) Método para aplicar um deslocamento de borda
CO2017011238A2 (es) Anticuerpos anti-c1s humanizados
BR112017008201A2 (pt) unidade de teste de gás, e, métodos para alaviar a adequabilidade e para determinar a possibilidade de um substrato.
WO2012151503A3 (en) Multiplexed genetic reporter assays and compositions
BR112016026136A2 (pt) composições e métodos para aumentar o desenvolvimento e o rendimento vegetal
BR112017018149A2 (pt) célula hospedeira recombinante, método para produzir uma proteína de interesse recombinante, sistema de expressão, e, processos para fabricar uma formulação de proteína estável e para reduzir a atividade de esterase em uma formulação de proteína.
BR112022009723A2 (pt) Anticorpos específicos para cd47, pd-l1, e usos dos mesmos
BR112017023624A2 (pt) método de cultivo em pequena escala para células em suspensão
BR112014004543A2 (pt) método de síntese de proteínas
BR112017027311A2 (pt) microorganismos de propagação & métodos relacionados & sistemas
BR112022006866A2 (pt) Métodos para caracterizar proteínas de células hospedeiras
WO2014052613A3 (en) Methods and compositions relating to next generation sequencing for genetic testing in alk related cancers

Legal Events

Date Code Title Description
B11A Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing
B11Y Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette]