CL2021002433A1 - Multiplexación de elementos reguladores para identificar elementos reguladores específicos al tipo de célula - Google Patents
Multiplexación de elementos reguladores para identificar elementos reguladores específicos al tipo de célulaInfo
- Publication number
- CL2021002433A1 CL2021002433A1 CL2021002433A CL2021002433A CL2021002433A1 CL 2021002433 A1 CL2021002433 A1 CL 2021002433A1 CL 2021002433 A CL2021002433 A CL 2021002433A CL 2021002433 A CL2021002433 A CL 2021002433A CL 2021002433 A1 CL2021002433 A1 CL 2021002433A1
- Authority
- CL
- Chile
- Prior art keywords
- regulatory elements
- cell type
- multiplexing
- identify
- specific
- Prior art date
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- C—CHEMISTRY; METALLURGY
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
- G01N33/5023—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on expression patterns
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/111—General methods applicable to biologically active non-coding nucleic acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14141—Use of virus, viral particle or viral elements as a vector
- C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
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- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Virology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Cell Biology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Toxicology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
En la presente se proporciona un método de alto rendimiento para analizar e identificar elementos reguladores que proporcionan expresión selectiva en un tipo de célula particular de interés. También se proporcionan composiciones de ácido nucleico utilizadas en el método de detección de alto rendimiento.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201962822528P | 2019-03-22 | 2019-03-22 |
Publications (1)
Publication Number | Publication Date |
---|---|
CL2021002433A1 true CL2021002433A1 (es) | 2022-09-20 |
Family
ID=72611757
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CL2021002433A CL2021002433A1 (es) | 2019-03-22 | 2021-09-20 | Multiplexación de elementos reguladores para identificar elementos reguladores específicos al tipo de célula |
Country Status (17)
Country | Link |
---|---|
US (1) | US20220170910A1 (es) |
EP (1) | EP3942055A4 (es) |
JP (1) | JP2022525477A (es) |
KR (1) | KR20210143855A (es) |
CN (1) | CN113874515A (es) |
AU (1) | AU2020245425A1 (es) |
BR (1) | BR112021018819A2 (es) |
CA (1) | CA3134501A1 (es) |
CL (1) | CL2021002433A1 (es) |
CO (1) | CO2021012576A2 (es) |
EA (1) | EA202192580A1 (es) |
IL (1) | IL286455A (es) |
MA (1) | MA55386A (es) |
MX (1) | MX2021011511A (es) |
SG (1) | SG11202110298RA (es) |
TW (1) | TW202102680A (es) |
WO (1) | WO2020198017A1 (es) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114085873A (zh) * | 2021-11-16 | 2022-02-25 | 珠海中科先进技术研究院有限公司 | 一种癌细胞状态辨识基因电路组及其制备方法 |
KR20240003760A (ko) * | 2022-06-29 | 2024-01-09 | 서울대학교산학협력단 | RNA 안정성 또는 mRNA 번역 증가용 신규 조절 엘리먼트,이와 상호작용하는 ZCCHC2, 및 이들의 용도 |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2879426A1 (en) * | 2013-01-14 | 2014-07-17 | Cellecta, Inc. | Methods and compositions for single cell expression profiling |
AU2015364636B9 (en) * | 2014-12-16 | 2021-12-02 | Board Of Regents Of The University Of Nebraska | Gene therapy for Juvenile Batten Disease |
SG11201909203WA (en) * | 2017-04-03 | 2019-11-28 | Encoded Therapeutics Inc | Tissue selective transgene expression |
-
2020
- 2020-03-20 KR KR1020217034275A patent/KR20210143855A/ko unknown
- 2020-03-20 BR BR112021018819A patent/BR112021018819A2/pt not_active Application Discontinuation
- 2020-03-20 MA MA055386A patent/MA55386A/fr unknown
- 2020-03-20 SG SG11202110298RA patent/SG11202110298RA/en unknown
- 2020-03-20 CA CA3134501A patent/CA3134501A1/en active Pending
- 2020-03-20 MX MX2021011511A patent/MX2021011511A/es unknown
- 2020-03-20 AU AU2020245425A patent/AU2020245425A1/en not_active Abandoned
- 2020-03-20 EP EP20777386.2A patent/EP3942055A4/en not_active Withdrawn
- 2020-03-20 JP JP2021556468A patent/JP2022525477A/ja active Pending
- 2020-03-20 EA EA202192580A patent/EA202192580A1/ru unknown
- 2020-03-20 US US17/442,057 patent/US20220170910A1/en active Pending
- 2020-03-20 CN CN202080037824.XA patent/CN113874515A/zh active Pending
- 2020-03-20 WO PCT/US2020/023881 patent/WO2020198017A1/en unknown
- 2020-03-20 TW TW109109448A patent/TW202102680A/zh unknown
-
2021
- 2021-09-19 IL IL286455A patent/IL286455A/en unknown
- 2021-09-20 CL CL2021002433A patent/CL2021002433A1/es unknown
- 2021-09-24 CO CONC2021/0012576A patent/CO2021012576A2/es unknown
Also Published As
Publication number | Publication date |
---|---|
EP3942055A1 (en) | 2022-01-26 |
EA202192580A1 (ru) | 2022-03-10 |
SG11202110298RA (en) | 2021-10-28 |
US20220170910A1 (en) | 2022-06-02 |
TW202102680A (zh) | 2021-01-16 |
MX2021011511A (es) | 2022-01-31 |
AU2020245425A1 (en) | 2021-10-07 |
CO2021012576A2 (es) | 2021-10-20 |
JP2022525477A (ja) | 2022-05-16 |
CA3134501A1 (en) | 2020-10-01 |
MA55386A (fr) | 2022-01-26 |
IL286455A (en) | 2021-12-01 |
KR20210143855A (ko) | 2021-11-29 |
WO2020198017A1 (en) | 2020-10-01 |
CN113874515A (zh) | 2021-12-31 |
EP3942055A4 (en) | 2022-12-28 |
BR112021018819A2 (pt) | 2021-11-23 |
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