BR112019006484A2 - método para detectar um ácido nucleico - Google Patents

método para detectar um ácido nucleico

Info

Publication number
BR112019006484A2
BR112019006484A2 BR112019006484A BR112019006484A BR112019006484A2 BR 112019006484 A2 BR112019006484 A2 BR 112019006484A2 BR 112019006484 A BR112019006484 A BR 112019006484A BR 112019006484 A BR112019006484 A BR 112019006484A BR 112019006484 A2 BR112019006484 A2 BR 112019006484A2
Authority
BR
Brazil
Prior art keywords
nucleic acid
detecting
cerium oxide
oxide support
acid
Prior art date
Application number
BR112019006484A
Other languages
English (en)
Inventor
Nakagawa Mai
Ito Masateru
Sawada Shinjiro
Sekiguchi Shota
Original Assignee
Toray Industries
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Toray Industries filed Critical Toray Industries
Publication of BR112019006484A2 publication Critical patent/BR112019006484A2/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/125—Specific component of sample, medium or buffer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/149—Particles, e.g. beads

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

método para detectar um ácido nucleico que compreende as seguintes etapas de: a) misturar uma solução contendo um ácido carboxílico e/ou um sal do mesmo, um suporte de óxido de cério e uma amostra contendo um ácido nucleico para adsorver o ácido nucleico ao suporte de óxido de cério, b) separar o suporte de óxido de cério no qual o ácido nucleico foi adsorvido a partir da mistura obtida na etapa a), c) coletar o ácido nucleico adicionando um eluente ao suporte de óxido de cério no qual o ácido nucleico foi adsorvido e que foi separado na etapa b), e d) detectar o ácido nucleico coletado na etapa c) por uma reação de hibridização ou uma reação de amplificação de ácido nucleico.
BR112019006484A 2016-11-10 2017-11-10 método para detectar um ácido nucleico BR112019006484A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2016219465 2016-11-10
PCT/JP2017/040505 WO2018088502A1 (ja) 2016-11-10 2017-11-10 核酸の検出方法

Publications (1)

Publication Number Publication Date
BR112019006484A2 true BR112019006484A2 (pt) 2019-10-15

Family

ID=62109884

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112019006484A BR112019006484A2 (pt) 2016-11-10 2017-11-10 método para detectar um ácido nucleico

Country Status (8)

Country Link
US (1) US11492655B2 (pt)
EP (1) EP3546590A4 (pt)
JP (1) JPWO2018088502A1 (pt)
KR (1) KR20190075074A (pt)
CN (1) CN110023506A (pt)
BR (1) BR112019006484A2 (pt)
CA (1) CA3039889A1 (pt)
WO (1) WO2018088502A1 (pt)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020102570A1 (en) * 2018-11-14 2020-05-22 Spectrum Solutions L.L.C. Rna preservation solution and methods of manufacture and use
US20210319852A1 (en) * 2019-01-31 2021-10-14 Precision Biomonitoring Inc. Computer-implemented method for determining survey sampling parameters for environmental nucleic acid
CN111060577B (zh) * 2019-12-31 2022-03-11 西南大学 一种消除lamp背景干扰的比率型电化学生物传感器、构建方法及应用

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5234809A (en) 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
US20020104801A1 (en) 1998-04-06 2002-08-08 Nicolas Voute Small dense microporous solid support materials, their preparation,and use for purification of large macromolecules and bioparticles
CN1223680C (zh) * 2002-12-10 2005-10-19 清华大学 扩增靶细胞或病毒的核酸的方法
WO2006015326A2 (en) * 2004-07-30 2006-02-09 Agencourt Bioscience Corporation Methods of isolating nucleic acids using multifunctional group-coated solid phase carriers
US20060223071A1 (en) * 2005-04-01 2006-10-05 Wisniewski Michele E Methods, compositions, and kits for detecting nucleic acids in a single vessel
JP2007006728A (ja) * 2005-06-28 2007-01-18 Bando Chem Ind Ltd 被覆無機粒子およびその利用
US20100129878A1 (en) * 2007-04-25 2010-05-27 Parthasarathy Ranjani V Methods for nucleic acid amplification
US20120003710A1 (en) * 2010-02-19 2012-01-05 Barbara Dawn Leinweber Isolation of Biomolecules from Biological Samples
JP6337470B2 (ja) * 2012-06-20 2018-06-06 東レ株式会社 核酸の検出方法および核酸検出キット
KR101546887B1 (ko) * 2013-06-24 2015-08-24 한국과학기술원 산화효소 활성을 가지는 산화세륨 나노입자를 이용한 핵산 및 생체물질 검출방법
CN108779455B (zh) * 2016-03-17 2022-09-27 东丽株式会社 核酸的回收方法

Also Published As

Publication number Publication date
JPWO2018088502A1 (ja) 2019-10-03
US20190276876A1 (en) 2019-09-12
KR20190075074A (ko) 2019-06-28
US11492655B2 (en) 2022-11-08
CA3039889A1 (en) 2018-05-17
EP3546590A4 (en) 2020-07-15
EP3546590A1 (en) 2019-10-02
WO2018088502A1 (ja) 2018-05-17
CN110023506A (zh) 2019-07-16

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Legal Events

Date Code Title Description
B11A Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing
B11Y Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette]
B350 Update of information on the portal [chapter 15.35 patent gazette]