BR112018070380A2 - método de germinação de esporos bacterianos, e, composição germinativa de nutriente. - Google Patents

método de germinação de esporos bacterianos, e, composição germinativa de nutriente.

Info

Publication number
BR112018070380A2
BR112018070380A2 BR112018070380A BR112018070380A BR112018070380A2 BR 112018070380 A2 BR112018070380 A2 BR 112018070380A2 BR 112018070380 A BR112018070380 A BR 112018070380A BR 112018070380 A BR112018070380 A BR 112018070380A BR 112018070380 A2 BR112018070380 A2 BR 112018070380A2
Authority
BR
Brazil
Prior art keywords
germination
composition
nutrient
germinal
spore
Prior art date
Application number
BR112018070380A
Other languages
English (en)
Inventor
Rosmarin Amanda
Greenwald Charles
Aberle Daniel
F K Everett Gabriel
Aboagye George
church Jordan
Pruitt Judy
Original Assignee
Nch Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nch Corp filed Critical Nch Corp
Publication of BR112018070380A2 publication Critical patent/BR112018070380A2/pt

Links

Classifications

    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66Microorganisms or materials therefrom
    • A61K35/74Bacteria
    • A61K35/741Probiotics
    • A61K35/742Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/065Microorganisms
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/02Inorganic compounds
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/34Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/34Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/348Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/04Preserving or maintaining viable microorganisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • C12N1/205Bacterial isolates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N3/00Spore forming or isolating processes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/01Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/01Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07Bacillus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/01Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07Bacillus
    • C12R2001/125Bacillus subtilis ; Hay bacillus; Grass bacillus

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Polymers & Plastics (AREA)
  • Mycology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Hydrology & Water Resources (AREA)
  • Environmental & Geological Engineering (AREA)
  • Water Supply & Treatment (AREA)
  • Food Science & Technology (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Molecular Biology (AREA)
  • Nutrition Science (AREA)
  • Inorganic Chemistry (AREA)
  • Physiology (AREA)
  • Animal Husbandry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

uma composição germinativa de nutriente para auxiliar na germinação de esporos e um método para aumentar a eficiência da germinação de esporos. a composição compreende l-aminoácidos, d-glicose e/ou d-frutose, um tampão de fosfato, um conservante industrial, e pode incluir esporos de bactérias ou eles podem ser combinados separadamente para germinação. o método compreende proporcionar uma composição germinativa de nutrientes e esporos de bactérias, preferivelmente de uma ou mais espécies bacillus, e aquecer a uma temperatura elevada preferida entre 41°c e 44°c por um período de incubação de cerca de 2 a 60 minutos. a composição germinativa de nutrientes está preferencialmente em uma forma líquida concentrada que é diluída imediatamente antes de iniciar o método de germinação/incubação no ponto de uso. o método também pode incluir a distribuição de uma solução de esporos germinados a um ponto de uso/consumo, tal como alimentação animal, água ou cama, ou um sistema de águas residuais ou dreno.
BR112018070380A 2016-04-05 2017-04-05 método de germinação de esporos bacterianos, e, composição germinativa de nutriente. BR112018070380A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662318587P 2016-04-05 2016-04-05
PCT/US2017/026122 WO2017176872A1 (en) 2016-04-05 2017-04-05 Nutrient rich germinant composition and spore incubation method

Publications (1)

Publication Number Publication Date
BR112018070380A2 true BR112018070380A2 (pt) 2019-02-05

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
BR112018070380A BR112018070380A2 (pt) 2016-04-05 2017-04-05 método de germinação de esporos bacterianos, e, composição germinativa de nutriente.

Country Status (12)

Country Link
US (2) US10610552B2 (pt)
EP (1) EP3440188A4 (pt)
JP (2) JP6953425B2 (pt)
KR (1) KR102498391B1 (pt)
CN (1) CN108884434B (pt)
BR (1) BR112018070380A2 (pt)
CA (1) CA3019732A1 (pt)
EC (1) ECSP18075371A (pt)
MX (1) MX2018012060A (pt)
MY (1) MY190858A (pt)
PH (1) PH12018502063A1 (pt)
WO (1) WO2017176872A1 (pt)

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Also Published As

Publication number Publication date
US10610552B2 (en) 2020-04-07
WO2017176872A1 (en) 2017-10-12
JP7248758B2 (ja) 2023-03-29
EP3440188A1 (en) 2019-02-13
US11484556B2 (en) 2022-11-01
MX2018012060A (es) 2019-03-06
US20200171104A1 (en) 2020-06-04
EP3440188A4 (en) 2019-10-16
US20170281696A1 (en) 2017-10-05
CN108884434A (zh) 2018-11-23
JP2022008620A (ja) 2022-01-13
ECSP18075371A (es) 2018-10-31
JP2019510496A (ja) 2019-04-18
MY190858A (en) 2022-05-12
CN108884434B (zh) 2023-06-09
KR20180131603A (ko) 2018-12-10
PH12018502063A1 (en) 2019-06-24
CA3019732A1 (en) 2017-10-12
KR102498391B1 (ko) 2023-02-13
JP6953425B2 (ja) 2021-10-27

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