BR112018069243A2 - proteína e gene tolerantes a herbicida, cassete de expressão, vetor recombinante, métodos para produzir uma proteína tolerante a herbicida, para aumentar a gama de herbicidas que podem ser tolerados, para selecionar células vegetais transformadas, para controlar ervas, para proteger uma planta, para conferir a uma planta tolerância a herbicida de sulfonilureia, para produzir e para cultivar uma planta que é tolerante a um herbicida de sulfonilureia, sistema de plantação, uso de uma proteína tolerante a herbicida. - Google Patents

proteína e gene tolerantes a herbicida, cassete de expressão, vetor recombinante, métodos para produzir uma proteína tolerante a herbicida, para aumentar a gama de herbicidas que podem ser tolerados, para selecionar células vegetais transformadas, para controlar ervas, para proteger uma planta, para conferir a uma planta tolerância a herbicida de sulfonilureia, para produzir e para cultivar uma planta que é tolerante a um herbicida de sulfonilureia, sistema de plantação, uso de uma proteína tolerante a herbicida.

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Publication number
BR112018069243A2
BR112018069243A2 BR112018069243A BR112018069243A BR112018069243A2 BR 112018069243 A2 BR112018069243 A2 BR 112018069243A2 BR 112018069243 A BR112018069243 A BR 112018069243A BR 112018069243 A BR112018069243 A BR 112018069243A BR 112018069243 A2 BR112018069243 A2 BR 112018069243A2
Authority
BR
Brazil
Prior art keywords
plant
herbicide
protein
tolerant protein
herbicide tolerant
Prior art date
Application number
BR112018069243A
Other languages
English (en)
Inventor
Tao Qing
Xie Xiangting
Bao Xiaoming
Original Assignee
Beijing Dabeinong Biotechnology Co Ltd
Beijing Dabeinong Tech Group
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beijing Dabeinong Biotechnology Co Ltd, Beijing Dabeinong Tech Group filed Critical Beijing Dabeinong Biotechnology Co Ltd
Publication of BR112018069243A2 publication Critical patent/BR112018069243A2/pt

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P15/00—Biocides for specific purposes not provided for in groups A01P1/00 - A01P13/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/32—Ingredients for reducing the noxious effect of the active substances to organisms other than pests, e.g. toxicity reducing compositions, self-destructing compositions
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
    • A01N47/36—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the group >N—CO—N< directly attached to at least one heterocyclic ring; Thio analogues thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
    • A01N47/46—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=C=S groups
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8278—Sulfonylurea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Environmental Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Agronomy & Crop Science (AREA)
  • Dentistry (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Mycology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

uma proteína tolerante a herbicida, sum1, um gene de codificação e um uso dos mesmos. a dita proteína tolerante a herbicida inclui: (a) uma proteína que tem uma sequência de aminoácidos conforme estabelecido na seq id no: 1; ou (b) uma proteína derivada de (a) através de substituição e/ou deleção e/ou adição de um ou mais aminoácidos na sequência de aminoácidos de (a) e que tem a atividade de hidrolase para tifensulfuron-metila. a proteína tolerante a herbicida pode exibir uma tolerância superior a múltiplos de herbicidas de sulfonilureia, pode ter tolerância de até quatro vezes a concentração de campo e, portanto, tem uma perspectiva de aplicação ampla em plantas.
BR112018069243A 2016-03-22 2016-12-09 proteína e gene tolerantes a herbicida, cassete de expressão, vetor recombinante, métodos para produzir uma proteína tolerante a herbicida, para aumentar a gama de herbicidas que podem ser tolerados, para selecionar células vegetais transformadas, para controlar ervas, para proteger uma planta, para conferir a uma planta tolerância a herbicida de sulfonilureia, para produzir e para cultivar uma planta que é tolerante a um herbicida de sulfonilureia, sistema de plantação, uso de uma proteína tolerante a herbicida. BR112018069243A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201610165121.0A CN105802933B (zh) 2016-03-22 2016-03-22 除草剂耐受性蛋白质、其编码基因及用途
PCT/CN2016/109176 WO2017161921A1 (zh) 2016-03-22 2016-12-09 除草剂耐受性蛋白质、其编码基因及用途

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Publication Number Publication Date
BR112018069243A2 true BR112018069243A2 (pt) 2019-01-22

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BR112018069243A BR112018069243A2 (pt) 2016-03-22 2016-12-09 proteína e gene tolerantes a herbicida, cassete de expressão, vetor recombinante, métodos para produzir uma proteína tolerante a herbicida, para aumentar a gama de herbicidas que podem ser tolerados, para selecionar células vegetais transformadas, para controlar ervas, para proteger uma planta, para conferir a uma planta tolerância a herbicida de sulfonilureia, para produzir e para cultivar uma planta que é tolerante a um herbicida de sulfonilureia, sistema de plantação, uso de uma proteína tolerante a herbicida.

Country Status (12)

Country Link
US (1) US10954528B2 (pt)
EP (1) EP3425046B1 (pt)
CN (1) CN105802933B (pt)
AR (1) AR107385A1 (pt)
AU (1) AU2016399292B2 (pt)
BR (1) BR112018069243A2 (pt)
CA (1) CA3018255C (pt)
DK (1) DK3425046T3 (pt)
ES (1) ES2902007T3 (pt)
MX (1) MX2018011581A (pt)
WO (1) WO2017161921A1 (pt)
ZA (1) ZA201806998B (pt)

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CN105802933B (zh) 2016-03-22 2020-05-05 北京大北农科技集团股份有限公司 除草剂耐受性蛋白质、其编码基因及用途
CN105746255B (zh) * 2016-03-22 2019-01-11 北京大北农科技集团股份有限公司 除草剂耐受性蛋白质的用途
CN105766992B (zh) * 2016-03-22 2018-06-22 北京大北农科技集团股份有限公司 除草剂耐受性蛋白质的用途
CN106755062A (zh) * 2016-11-17 2017-05-31 北京大北农科技集团股份有限公司 使用磺酰脲类除草剂水解酶基因作为选择标记物的转化方法
CN106755061A (zh) * 2016-11-17 2017-05-31 北京大北农科技集团股份有限公司 使用磺酰脲类除草剂水解酶基因作为选择标记物的转化方法
CN107099548B (zh) * 2017-05-09 2020-11-03 北京大北农生物技术有限公司 提高大豆转化效率的方法
CN108330116B (zh) * 2018-02-07 2020-05-05 北京大北农生物技术有限公司 除草剂耐受性蛋白质、其编码基因及用途
GB2578509A (en) * 2019-08-30 2020-05-13 Rotam Agrochem Int Co Ltd Method for controlling growth of glyphosate-tolerant plants
CN110628733B (zh) * 2019-09-17 2021-07-30 北京大北农生物技术有限公司 除草剂耐受性蛋白质、其编码基因及用途
CN111139248B (zh) * 2019-10-14 2021-07-13 中国农业科学院生物技术研究所 dicX5基因及其对除草剂的降解功能
CN110872591B (zh) * 2019-10-14 2021-07-13 中国农业科学院生物技术研究所 除草剂麦草畏降解基因dicX1及其应用
CN111139249B (zh) * 2019-10-14 2021-07-09 中国农业科学院生物技术研究所 运动金球菌麦草畏降解基因dicM的用途
CN111118037B (zh) * 2019-10-14 2021-07-13 中国农业科学院生物技术研究所 具有除草剂麦草畏降解功能的基因dicx4及其应用
CN115340987B (zh) * 2021-05-12 2023-12-01 北京大北农生物技术有限公司 除草剂耐受性蛋白质、其编码基因及用途

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EP3425046A1 (en) 2019-01-09
MX2018011581A (es) 2019-06-24
AU2016399292A1 (en) 2018-11-15
ES2902007T3 (es) 2022-03-24
EP3425046A4 (en) 2019-08-28
US20190106705A1 (en) 2019-04-11
AU2016399292B2 (en) 2021-09-09
CN105802933B (zh) 2020-05-05
ZA201806998B (en) 2020-01-29
AR107385A1 (es) 2018-04-25
CA3018255C (en) 2022-07-05
US10954528B2 (en) 2021-03-23
DK3425046T3 (da) 2022-01-03
WO2017161921A1 (zh) 2017-09-28
CA3018255A1 (en) 2017-09-28
EP3425046B1 (en) 2021-12-01
CN105802933A (zh) 2016-07-27

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