BR112018068808A2 - uso de proteína tolerante a herbicida - Google Patents
uso de proteína tolerante a herbicidaInfo
- Publication number
- BR112018068808A2 BR112018068808A2 BR112018068808A BR112018068808A BR112018068808A2 BR 112018068808 A2 BR112018068808 A2 BR 112018068808A2 BR 112018068808 A BR112018068808 A BR 112018068808A BR 112018068808 A BR112018068808 A BR 112018068808A BR 112018068808 A2 BR112018068808 A2 BR 112018068808A2
- Authority
- BR
- Brazil
- Prior art keywords
- hydrolase
- herbicide
- present
- tifensulfuron
- nucleotide sequence
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
- C12N15/8278—Sulfonylurea
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M7/00—Special adaptations or arrangements of liquid-spraying apparatus for purposes covered by this subclass
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/54—1,3-Diazines; Hydrogenated 1,3-diazines
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
- A01N47/36—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the group >N—CO—N< directly attached to at least one heterocyclic ring; Thio analogues thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
- A01N57/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds
- A01N57/20—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds containing acyclic or cycloaliphatic radicals
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
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- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/795—Porphyrin- or corrin-ring-containing peptides
- C07K14/80—Cytochromes
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
- C12N15/8275—Glyphosate
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
- C12N15/8289—Male sterility
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1022—Transferases (2.) transferring aldehyde or ketonic groups (2.2)
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
- C12N9/1092—3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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- C12Y—ENZYMES
- C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
- C12Y103/03—Oxidoreductases acting on the CH-CH group of donors (1.3) with oxygen as acceptor (1.3.3)
- C12Y103/03004—Protoporphyrinogen oxidase (1.3.3.4)
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- C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
- C12Y113/11—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of two atoms of oxygen (1.13.11)
- C12Y113/11027—4-Hydroxyphenylpyruvate dioxygenase (1.13.11.27)
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- C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
- C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
- C12Y113/12019—2-Oxuglutarate dioxygenase (ethylene-forming) (1.13.12.19)
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- C12Y202/00—Transferases transferring aldehyde or ketonic groups (2.2)
- C12Y202/01—Transketolases and transaldolases (2.2.1)
- C12Y202/01006—Acetolactate synthase (2.2.1.6)
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- C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
- C12Y205/01019—3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase
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- Life Sciences & Earth Sciences (AREA)
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- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
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- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
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- Botany (AREA)
- Toxicology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
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Abstract
a presente invenção refere-se ao uso de uma proteína tolerante a herbicida, em que o método para controlar ervas daninhas compreende aplicar um herbicida contendo uma dose eficaz de sulfometuron-metila a um ambiente de crescimento de plantas, em que pelo menos uma planta transgênica está presente, em que a planta transgênica compreende uma sequência de nucleotídeos que codifica uma tifensulfuron hidrolase no seu genoma e, comparada com outras plantas sem a sequência de nucleotídeos que codifica a hidrolase, a planta transgênica tem dano à planta reduzido e/ou aumento do rendimento vegetal. a presente invenção divulga, pela primeira vez, que uma tifensulfuron hidrolase pode apresentar uma alta tolerância a um herbicida de sulfometuron-metil, plantas contendo uma sequência de nucleotídeos que codifica a tifensulfuron hidrolase são fortemente tolerantes ao herbicida sulfometuron-metila e podem tolerar pelo menos uma vez a concentração de campo e, portanto, a hidrolase tem ampla perspectiva de aplicação em plantas.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
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CN201610164721.5A CN105724139B (zh) | 2016-03-22 | 2016-03-22 | 除草剂耐受性蛋白质的用途 |
PCT/CN2016/108410 WO2017161915A1 (zh) | 2016-03-22 | 2016-12-02 | 除草剂耐受性蛋白质的用途 |
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BR112018068808A2 true BR112018068808A2 (pt) | 2019-01-22 |
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BR112018068808A BR112018068808A2 (pt) | 2016-03-22 | 2016-12-02 | uso de proteína tolerante a herbicida |
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US (1) | US20190055576A1 (pt) |
CN (1) | CN105724139B (pt) |
BR (1) | BR112018068808A2 (pt) |
WO (1) | WO2017161915A1 (pt) |
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CN105724139B (zh) * | 2016-03-22 | 2018-10-30 | 北京大北农科技集团股份有限公司 | 除草剂耐受性蛋白质的用途 |
CN105746255B (zh) * | 2016-03-22 | 2019-01-11 | 北京大北农科技集团股份有限公司 | 除草剂耐受性蛋白质的用途 |
CN105766992B (zh) * | 2016-03-22 | 2018-06-22 | 北京大北农科技集团股份有限公司 | 除草剂耐受性蛋白质的用途 |
CN105802933B (zh) * | 2016-03-22 | 2020-05-05 | 北京大北农科技集团股份有限公司 | 除草剂耐受性蛋白质、其编码基因及用途 |
CN107099548B (zh) | 2017-05-09 | 2020-11-03 | 北京大北农生物技术有限公司 | 提高大豆转化效率的方法 |
CN115404197A (zh) * | 2022-09-16 | 2022-11-29 | 河南省农业科学院经济作物研究所 | 一种油莎豆原生质体的制备方法 |
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CN101451143A (zh) * | 2008-12-25 | 2009-06-10 | 浙江大学 | 一种抗除草剂基因及其应用 |
TWI667347B (zh) * | 2010-12-15 | 2019-08-01 | 瑞士商先正達合夥公司 | 大豆品種syht0h2及偵測其之組合物及方法 |
CN102286501B (zh) * | 2011-07-25 | 2012-12-12 | 南京农业大学 | 噻吩磺隆水解酶基因tsmE及其应用 |
CN103013938B (zh) * | 2012-12-25 | 2014-12-17 | 北京大北农科技集团股份有限公司 | 除草剂抗性蛋白质、其编码基因及用途 |
CN103013939B (zh) * | 2012-12-25 | 2015-01-21 | 北京大北农科技集团股份有限公司 | 除草剂抗性蛋白质、其编码基因及用途 |
CN104611307B (zh) * | 2015-02-13 | 2018-04-27 | 北京大北农科技集团股份有限公司 | 除草剂抗性蛋白质、其编码基因及用途 |
CN104611306B (zh) * | 2015-02-13 | 2019-10-18 | 北京大北农科技集团股份有限公司 | 除草剂抗性蛋白质、其编码基因及用途 |
CN104611308B (zh) * | 2015-02-13 | 2019-01-11 | 北京大北农科技集团股份有限公司 | 除草剂抗性蛋白质、其编码基因及用途 |
CN105724139B (zh) * | 2016-03-22 | 2018-10-30 | 北京大北农科技集团股份有限公司 | 除草剂耐受性蛋白质的用途 |
CN105746255B (zh) * | 2016-03-22 | 2019-01-11 | 北京大北农科技集团股份有限公司 | 除草剂耐受性蛋白质的用途 |
CN105766992B (zh) * | 2016-03-22 | 2018-06-22 | 北京大北农科技集团股份有限公司 | 除草剂耐受性蛋白质的用途 |
CN105724140B (zh) * | 2016-03-22 | 2019-04-05 | 北京大北农科技集团股份有限公司 | 除草剂耐受性蛋白质的用途 |
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2016
- 2016-03-22 CN CN201610164721.5A patent/CN105724139B/zh active Active
- 2016-12-02 US US16/079,252 patent/US20190055576A1/en not_active Abandoned
- 2016-12-02 WO PCT/CN2016/108410 patent/WO2017161915A1/zh active Application Filing
- 2016-12-02 BR BR112018068808A patent/BR112018068808A2/pt not_active Application Discontinuation
Also Published As
Publication number | Publication date |
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CN105724139B (zh) | 2018-10-30 |
WO2017161915A1 (zh) | 2017-09-28 |
US20190055576A1 (en) | 2019-02-21 |
CN105724139A (zh) | 2016-07-06 |
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