BR112022017704A2 - Método e sistema de edição de genoma multiplex - Google Patents

Método e sistema de edição de genoma multiplex

Info

Publication number
BR112022017704A2
BR112022017704A2 BR112022017704A BR112022017704A BR112022017704A2 BR 112022017704 A2 BR112022017704 A2 BR 112022017704A2 BR 112022017704 A BR112022017704 A BR 112022017704A BR 112022017704 A BR112022017704 A BR 112022017704A BR 112022017704 A2 BR112022017704 A2 BR 112022017704A2
Authority
BR
Brazil
Prior art keywords
genome editing
editing method
multiplex genome
relates
multiplex
Prior art date
Application number
BR112022017704A
Other languages
English (en)
Inventor
Gao Caixia
Li Chao
Chen Kunling
Original Assignee
Suzhou Qi Biodesign Biotechnology Company Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Suzhou Qi Biodesign Biotechnology Company Ltd filed Critical Suzhou Qi Biodesign Biotechnology Company Ltd
Publication of BR112022017704A2 publication Critical patent/BR112022017704A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]

Abstract

MÉTODO E SISTEMA DE EDIÇÃO DE GENOMA MULTIPLEX. A presente invenção refere-se ao campo de engenharia genética de plantas. Em particular, a invenção refere-se a um método e a um sistema de edição de genoma multiplex aplicável a plantas, especialmente cultura cultivada. Mais particularmente, a presente invenção refere-se a método e sistema que são baseados em nickase de CRISPR, e permite que vários tipos de edição de genoma sejam realizados simultaneamente.
BR112022017704A 2020-03-04 2021-03-04 Método e sistema de edição de genoma multiplex BR112022017704A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN202010143643 2020-03-04
PCT/CN2021/079087 WO2021175289A1 (zh) 2020-03-04 2021-03-04 多重基因组编辑方法和系统

Publications (1)

Publication Number Publication Date
BR112022017704A2 true BR112022017704A2 (pt) 2022-11-01

Family

ID=77612853

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112022017704A BR112022017704A2 (pt) 2020-03-04 2021-03-04 Método e sistema de edição de genoma multiplex

Country Status (5)

Country Link
US (1) US20240117368A1 (pt)
EP (1) EP4116426A1 (pt)
CN (1) CN115667528A (pt)
BR (1) BR112022017704A2 (pt)
WO (1) WO2021175289A1 (pt)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20230265405A1 (en) * 2022-02-22 2023-08-24 Massachusetts Institute Of Technology Engineered nucleases and methods of use thereof
CN114686456B (zh) * 2022-05-10 2023-02-17 中山大学 基于双分子脱氨酶互补的碱基编辑系统及其应用
CN116751799B (zh) * 2023-06-14 2024-01-26 江南大学 一种多位点双重碱基编辑器及其应用

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10287590B2 (en) * 2014-02-12 2019-05-14 Dna2.0, Inc. Methods for generating libraries with co-varying regions of polynuleotides for genome modification
WO2016054106A1 (en) * 2014-09-29 2016-04-07 The Regents Of The University Of California SCAFFOLD RNAs
WO2016061481A1 (en) * 2014-10-17 2016-04-21 The Penn State Research Foundation Methods and compositions for multiplex rna guided genome editing and other rna technologies
WO2017011721A1 (en) * 2015-07-15 2017-01-19 Rutgers, The State University Of New Jersey Nuclease-independent targeted gene editing platform and uses thereof
CN106191099A (zh) * 2016-07-27 2016-12-07 苏州泓迅生物科技有限公司 一种基于CRISPR‑Cas9系统的酿酒酵母基因组并行多重编辑载体及其应用
WO2018071868A1 (en) * 2016-10-14 2018-04-19 President And Fellows Of Harvard College Aav delivery of nucleobase editors
CN107043779B (zh) * 2016-12-01 2020-05-12 中国农业科学院作物科学研究所 一种CRISPR/nCas9介导的定点碱基替换在植物中的应用
JP7219972B2 (ja) * 2017-01-05 2023-02-09 ラトガース，ザ ステート ユニバーシティ オブ ニュー ジャージー Ｄｎａ二本鎖切断に非依存的な標的化遺伝子編集プラットフォームおよびその用途
CN110612353A (zh) * 2017-03-03 2019-12-24 加利福尼亚大学董事会 经由抑制性tRNAs和脱氨酶对突变进行RNA靶向
CN111836825A (zh) * 2018-01-11 2020-10-27 科沃施种子欧洲股份两合公司 优化的植物crispr/cpf1系统
CN110066824B (zh) * 2018-01-24 2021-06-08 中国农业科学院植物保护研究所 一套用于水稻的碱基编辑人工系统
US11739322B2 (en) * 2018-02-01 2023-08-29 Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences Method for genome editing using a self-inactivating CRISPR nuclease
WO2019153902A1 (zh) * 2018-02-11 2019-08-15 中国科学院上海生命科学研究院 植物基因组定点替换的方法
CN110527695B (zh) * 2019-03-07 2020-06-16 山东舜丰生物科技有限公司 一种用于基因定点突变的核酸构建物

Also Published As

Publication number Publication date
CN115667528A (zh) 2023-01-31
EP4116426A1 (en) 2023-01-11
WO2021175289A1 (zh) 2021-09-10
US20240117368A1 (en) 2024-04-11

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