BR112017017494A2 - protease imobilizada com resistência melhorada à mudança no ambiente externo - Google Patents

protease imobilizada com resistência melhorada à mudança no ambiente externo

Info

Publication number
BR112017017494A2
BR112017017494A2 BR112017017494A BR112017017494A BR112017017494A2 BR 112017017494 A2 BR112017017494 A2 BR 112017017494A2 BR 112017017494 A BR112017017494 A BR 112017017494A BR 112017017494 A BR112017017494 A BR 112017017494A BR 112017017494 A2 BR112017017494 A2 BR 112017017494A2
Authority
BR
Brazil
Prior art keywords
protease
change
external environment
immobilized
immobilized protease
Prior art date
Application number
BR112017017494A
Other languages
English (en)
Inventor
Iwamoto Noriko
Shimada Takashi
Original Assignee
Shimadzu Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shimadzu Corp filed Critical Shimadzu Corp
Publication of BR112017017494A2 publication Critical patent/BR112017017494A2/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6427—Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21004—Trypsin (3.4.21.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/2105—Lysyl endopeptidase (3.4.21.50)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Inorganic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

a presente invenção refere-se a uma protease altamente ativa que pode ser usada na preparação da amostra para espectrometria de massa de uma proteína e tem excelente estabilidade contra uma mudança em um ambiente externo. a presente invenção provê uma protease imobilizada, caracterizada pelo fato de que uma protease purificada cruamente ou uma que não tenha sido submetida a um tratamento de resistência à autodigestão é imobilizada em superfícies de nanopartículas, e provê um método para produzir a protease imobilizada. a protease imobilizada da presente invenção pode manter alta atividade sem ser submetida a uma mudança em um ambiente externo e, portanto, é eficaz, por exemplo, na preparação de uma amostra para ser fornecida por espectrometria de massa de uma proteína em um espécime clínico.
BR112017017494A 2015-03-09 2015-12-18 protease imobilizada com resistência melhorada à mudança no ambiente externo BR112017017494A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2015046380 2015-03-09
PCT/JP2015/085444 WO2016143223A1 (ja) 2015-03-09 2015-12-18 外部環境変化に対する耐性が向上した固定化プロテアーゼ

Publications (1)

Publication Number Publication Date
BR112017017494A2 true BR112017017494A2 (pt) 2018-04-17

Family

ID=56879446

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112017017494A BR112017017494A2 (pt) 2015-03-09 2015-12-18 protease imobilizada com resistência melhorada à mudança no ambiente externo

Country Status (7)

Country Link
US (1) US20180051272A1 (pt)
EP (1) EP3269807A4 (pt)
JP (1) JPWO2016143223A1 (pt)
KR (1) KR20170120691A (pt)
CN (1) CN107406841A (pt)
BR (1) BR112017017494A2 (pt)
WO (1) WO2016143223A1 (pt)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111512152B (zh) * 2017-12-28 2023-08-11 株式会社岛津制作所 改善单克隆抗体的检测结果的方法
JP6835260B2 (ja) * 2017-12-28 2021-02-24 株式会社島津製作所 モノクローナル抗体の簡素化された定量方法
EP4365586A1 (en) 2021-06-23 2024-05-08 Shimadzu Corporation Method for analyzing antibody
CN114672475B (zh) * 2022-03-29 2024-02-06 广东海洋大学 一种固定化碱性蛋白酶及其制备方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7166441B2 (en) * 2002-03-12 2007-01-23 Perseptive Biosystems Inc. Method and apparatus for the identification and quantification of biomolecules
JP2004305021A (ja) * 2003-04-02 2004-11-04 Asahi Denka Kogyo Kk 酵素処理卵黄の製造方法
CN100363491C (zh) * 2005-07-28 2008-01-23 浙江大学 纳米固定化酶体外定向生产tf2a粗提物的方法
EP1988928A4 (en) * 2006-02-24 2011-11-16 Univ Yonsei Iacf MAGNETIC NANOCOMPOSITE FOR CONTRAST, INTELLIGENT CONTRAST, DRUG DELIVERY FOR SIMULTANEOUS DIAGNOSIS AND TREATMENT AND DISPOSAL FOR TARGET SUBSTANCE
DK2417262T3 (da) * 2009-04-07 2015-06-22 Dow Agrosciences Llc Nanopartikelmedieret tilførsel af sekvensspecifikke nukleaser
US10081542B2 (en) * 2010-04-20 2018-09-25 University of Florida Research Foundation, lnc. Nanozymes, methods of making nanozymes, and methods of using nanozymes
CN103882002B (zh) * 2014-01-16 2016-10-19 中国人民解放军军事医学科学院放射与辐射医学研究所 一种固定化蛋白酶试剂的制备及其应用
CN103887030A (zh) * 2014-04-04 2014-06-25 华东理工大学 一种用于纯化并固定化组氨酸标签蛋白的磁性亲和纳米颗粒及其制备方法和应用

Also Published As

Publication number Publication date
EP3269807A4 (en) 2018-08-08
JPWO2016143223A1 (ja) 2017-12-28
CN107406841A (zh) 2017-11-28
EP3269807A1 (en) 2018-01-17
WO2016143223A1 (ja) 2016-09-15
US20180051272A1 (en) 2018-02-22
KR20170120691A (ko) 2017-10-31

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B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements