BR112017007923A2 - método para produzir manipulação genética mediada por reações multiplex com rna em uma célula receptora, construção de ácido nucleico, cassete de expressão, vetor, célula receptora, célula geneticamente modificada, tecido, planta ou animal, progênie de planta ou animal e método para modificar um sítio alvo no genoma de uma célula - Google Patents

método para produzir manipulação genética mediada por reações multiplex com rna em uma célula receptora, construção de ácido nucleico, cassete de expressão, vetor, célula receptora, célula geneticamente modificada, tecido, planta ou animal, progênie de planta ou animal e método para modificar um sítio alvo no genoma de uma célula

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BR112017007923A2
BR112017007923A2 BR112017007923A BR112017007923A BR112017007923A2 BR 112017007923 A2 BR112017007923 A2 BR 112017007923A2 BR 112017007923 A BR112017007923 A BR 112017007923A BR 112017007923 A BR112017007923 A BR 112017007923A BR 112017007923 A2 BR112017007923 A2 BR 112017007923A2
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cell
rna
plant
animal
receptor
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BR112017007923A
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BR112017007923B1 (pt
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Xie Kabin
Yang Yinong
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Penn State Res Found
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2330/50—Biochemical production, i.e. in a transformed host cell
    • C12N2330/51—Specially adapted vectors

Abstract

a invenção inclui materiais e métodos para gerar numerosos pequenos rnas a partir de uma construção polinucleotídica (gene sintético) para facilitar a reação multiplex guiada por rna na edição de genoma, modificação, inibição de expressão e outras tecnologias com base no rna. a construção do gene/polinucleotídeo sintético codifica componentes de rna policistrônicos separados pelo rnat e, preferencialmente, também inclui componentes regulatórios, tais como um promotor ou terminador para formar um cassete de expressão. uma vez transcrito em uma célula, o transcrito é processado pela célula a múltiplas moléculas de rna pelo sistema de processamento endógeno de rnat. o sistema pode ser aplicado para qualquer método de manipulação de genes baseado no rna, incluindo a edição de genoma mediada por rna, silenciamento de genes mediado por microrna artificial, manipulação genética por rna mediada por pequenos rnas, silenciamento de genes de rna dupla fita, mecanismos antisense e semelhantes.
BR112017007923-2A 2014-10-17 2015-10-16 Método para produzir manipulação genética mediada por reações multiplex com rna em uma célula receptora, construção de ácido nucleico,cassete de expressão, vetor, célula receptora e célula geneticamente modificada BR112017007923B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462065093P 2014-10-17 2014-10-17
US62/065,093 2014-10-17
PCT/US2015/055980 WO2016061481A1 (en) 2014-10-17 2015-10-16 Methods and compositions for multiplex rna guided genome editing and other rna technologies

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BR112017007923A2 true BR112017007923A2 (pt) 2018-03-20
BR112017007923B1 BR112017007923B1 (pt) 2023-12-12

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US (2) US10308947B2 (pt)
EP (1) EP3207139A1 (pt)
CN (1) CN107027313B (pt)
BR (1) BR112017007923B1 (pt)
CA (1) CA2964796C (pt)
WO (1) WO2016061481A1 (pt)

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